• KSBB Journal
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• 제15권4호
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• pp.398-401
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• 2000

Taxus chinensis 식물세포 배양에 의한 paclitaxel대량 생산의 경우 biomass내 paclitaxel이외에 다른 많은 유도체들이 확 인 되었다. 이를 목표 물질로 선정하여 Prep-LC를 이용하여 분리/정제하고 NMR, MS 등으로 화학구조분석을 하여 이들 물질들에 대한 동정을 하였다. 또한 Taxus chinensis 식물세포 배양에 의한 paclitaxel 대량 생산의 경우 paclitaxel 생산량에 따른 여러가지 유도체들의 생산 경향을 조사하였으며, paclitaxel의 생산량이 증가할 수록 유도체들의 생산량은 상대 적으로 감소함을 알 수 있었다. 이러한 연구는 결국 paclitaxel 이외의 새로운 유용성분 및 전구체 확인, 분리/정제 방법의 개발, paclitaxel 생산에서의 품질관리에 유용하게 사용되어 질 수 있다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.202-205
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• 2008

Anaerobic digestion(AD) is the most promising method of treating and recycling of different organic wastes, such as OFMSW, household wastes, animal manure, agro-industrial wastes, industrial organic wastes and sewage sludge. During AD, i.e. degradation in the absence of oxygen, organic material is decomposed by anaerobes forming degestates such as an excellent fertilizer and biogas, a mixture of carbon dioxide and methane. AD has been one of the leading technologies that can make a large contribution to producing renewable energy and to reducing $CO_2$ and other GHG emission, it is becoming a key method for both waste treatment and recovery of a renewable fuel and other valuable co-products. A classification of the basic AD technologies for the production of biogas can be made according to the dry matter of biowaste and digestion temperature, which divide the AD process in wet and dry, mesophilic and thermophilic. The biogas produced from AD plant can be utilized as an alternative energy source, for lighting and cooking in case of small-scale, for CHP and vehicle fuel or fuel in industrials in case of large-scale. This paper provides an overview of the status of biogas production and utilization technologies.

• 한국초전도ㆍ저온공학회논문지
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• 제18권3호
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• pp.1-5
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• 2016

Ion-beam assisted deposition (IBAD) technology has been successfully applied to high-temperature superconductor coated conductors (CC) as textured substrates. Since the coated conductors were proposed as a potential framework for utilizing the superior transport characteristics of $YBa_2Cu_3O_7$ and related cuprate oxides, several methods including rolling-assisted bi-axial textured substrates (RABiTS) and inclined substrate deposition (ISD), as well as IBAD, have been attempted. As of 2016, most companies that are trying to commercialize CC adapt IBAD technology except for American Superconductors who use RABiTS predominantly. For the materials in the IBAD process, initial efforts to use yttria-stabilized zirconia (YSZ) or related fluorites in Fujikura in Japan have quickly given way to MgO which technique was developed by Stanford University in the USA. In this review, we present a historical overview of IBAD technology, in particular, for the application of CC. We describe the key scientific understanding of nucleation, the texturing mechanism, and the growth of large bi-axial grains and discuss some potential new IBAD materials and systems for large-scale production.

• 한국정밀공학회지
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• 제23권8호
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• pp.178-184
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• 2006

Hot embossing is to fabricate desired pattern on the polymer substrate by pressing the patterned mold against the substrate which is heated above the glass transition temperature, and it is a high throughput fabrication method for bio chip, optical microstructure, etc. due to the simultaneous large area patterning. However, the bad pattern fidelity in large area patterning is one of the obstacles to applying the hot embossing technology for mass production. In the present study, PDMS pad was used as a cushion on the backside of the micro-patterned 4 inch Si mold to improve the pattern fidelity over the 4 inch PMMA sheet by increasing the conformal contact between the Si mold and the PMMA sheet. The pattern replicability improvement over 4 inch wafer scale was evaluated by comparing the replicated pattern height and depth for PDMS-cushioned Si mold against the rigid Si mold without PDMS cushion.

• 한국측량학회지
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• 제13권1호
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• pp.77-83
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• 1995

수치지도 제작의 일반화 기법은 대축척 실측 지도를 이용한 소축척 편집 지도의 자동 제작과 수치 지도 데이터 베이스 구축 등의 활용 분야에 필수적 요소이다. 본 연구는 수작업으로 처리되고 있는 편집도의 제작을 자동화하기 위한 방안으로 디지탈 환경하에서의 일반화 기법에 대한 연구로서 Zero-Crossing알고리즘을 국립지리원 발행 l/5,000지도 6도엽에 포함되어 있는 전남 여수 일대의 해안선에 적용, 다양한 단순화 허용 범위에 의해 수치 지도를 제작하여 편집도와 도해적인 표현 방법에 대한 문제점을 비교 분석하였다. 또한, Zero-Crossing알고리즘에 의한 단순화의 효용성을 입증하기 위해 Douglas-Peucker알고리즘을 이용한 단순화 결과와 비교 분석을 하였으며, 단순화의 허용 범위별로 일반화가 이루어진 수치 지도와 수작업으로 제작된 편집도와의 비교를 통해 일반화된 Line에 대한 평가를 도출하고, 단순화 허용 범위의 선택에 따른 효율성을 판단하기 위해 일반화 처리 결과의 데이타 용량을 분석하였다.

• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.1093-1100
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• 2015

Acetate and lactate in growth media are detrimental to the production of Thermus maltogenic amylase (ThMA), a heterologous protein, as well as to the growth of recombinant Escherichia coli. Only 50 mM of acetate or 10 mM of lactate reduced 90% of specific ThMA activity. In this study, mutant E. coli strains blocked in the ackA-pta or ackA-pta and ldh pathways were created, characterized, and assessed for their culture performace in 300 L-scale fermentation. The ackApta and ldh double-mutant strain formed significantly less lactate and acetate, and produced a concomitant increase in the excretion of pyruvate (17.8 mM) under anaerobic conditions. The ackA-pta mutant strain accumulated significant acetate but had an approximately 2-fold increase in the formation of lactate. The ackA-pta and ldh double-mutant strain had superior overall performance in large-scale culture under suboptimal conditions, giving 67% higher cell density and 66% higher ThMA activity compared with those of the control strain. The doublemutant strain also achieved a 179% improvement in volumetric ThMA production.

• 한국임상수의학회지
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• 제16권1호
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• pp.163-176
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• 1999

The nuclear transplantation technique is known as the most potential and efficient method for producing large numbers of genetically identical animals from a single embryo and somatic cells. After Dolly was introduced in 1997, many scientists were amazed. A possibility came to a reality that live offspring could be produced with differentiated somatic cells from an adult animal. On the other side, many in the press and the sensationalists focused on the socially, ethically and scientifically unacceptable sides of the technology. In this article, the history, current status and prospects of the technological development of nuclear transplantation in mammals and its application to the production of cloned animals are described. For the efficient and successful production of cloned embryos by nuclear transplantation, the right selection, preactivation and micromanipulation of oocytes as capacious recipient cytoplasm, the adequate and benefitial preparation of multiple totipotent embryonic and somatic cells as donor nuclei, fusion of them and in vitro production of cloned embryos are very critical. Recently the overall efficiency of production of cloned embryos and offspring in livestock has been much improved. Cloning will also be a more efficient, faster and useful way of creating transgenic fetuses for gene therapies, gene pharming, organs for xenotransplantation by preselection and mass production of transgenic embryos and consequently improving the production efficiency in transgenic animals. Further technical development of nuclear transplantation will enable large-scale production of cloned livestock and in near future the commercial cloning of animals will become a reality.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2008년도 춘계 종합학술대회 논문집
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• pp.198-202
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• 2008

대규모 3D 컴퓨터애니메이션의 제작공정은 제작기술의 발전과 예산의 대규모화로 인해 그 구조가 더욱 복잡화, 세분화, 되고 있으며, 프로덕션타입, 제작규모, 제작방식과 같은 유형에 따라 제작 파이프라인의 형태가 다양하게 변화되고 있다. 하지만, 소규모 제작업체에서는 제작 파이프라인의 개념과 그 구성에 대한 변화와 발전이 미비하여 이에 대한 재조명이 필요할 때이다. 이러한 연구의 배경을 주목하여 본 연구는 국내 소규모 3D애니메이션의 제작 파이프라인에 대한 이해를 돕고, 소규모 국내 프로덕션 실정에 맞는 3D애니메이션 제작 파이프라인의 구성 방안을 모색 하고자하는데 그 목적을 가진다. 본 연구에서 제시하는 소규모 3D애니메이션의 제작 파이프라인 구성 모델과 방법론은 프로젝트의 성향이나 주변 환경에 따라 충분히 변화 가능한 것이기 때문에 절대적인 것만은 아니며, 3D 컴퓨터애니메이션 제작 파이프라인의 구성을 위해서는 그 목적을 충분히 이해하고, 프로덕션내의 작업자간의 정보의 공유가 충분히 이루어질 수 있도록 소규모 프로덕션의 환경을 고려하여 상황에 맞추어 구성 할 수 있는 방법이 될 것이라고 생각한다.

• 대한산업공학회지
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• 제29권4호
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• pp.334-344
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• 2003

A mixture experiment is a special type of response surface experiment in which factors are the ingredients or components of a mixture, and the response is a function of the proportions of each ingredient. Evolutionary operation is useful to improve on-line full-scale manufacturing process by systematically changing the levels of the process variables without jeopardizing the product. This paper presents an evolutionary operation procedure with mixture variables for large-scale mixture production process which can be beneficial to practitioners who should improve on-line mixture quality while maintaining the production amount of the mixture product.

• 농촌지도와개발
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• 제24권1호
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• pp.1-8
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• 2017

The Purpose of this study is to predict farm size per farming household in Chungcheongnam-province by using the Markov chains and Quadratic Programming.. The results are as follows; First, small-scale farms with less than 1.0ha of land are predicted to be still more than half (of total farming households) in 2025 as well. Second, large-scale farms with 3.0ha-5.0ha land and extra large-scale farms with over 5.0ha of land are predicted to gradually expand their proportion in total farm scale. Third, middle-scale farms with 1.0ha-3.0ha land are forecasted to be reduced in their relative proportion. It is required to take into account regional characteristics to improve the effectiveness of a rice industry policy. Therefore, this study has some significance in attempting to research on the ownership structure of rice production areas in consideration of target regions.