• KSBB Journal
• /
• v.15 no.4
• /
• pp.398-401
• /
• 2000

Taxiods inclusive paclitaxel were produced isolated and purified from plant cell cultures of Taxus chinensis in large-scale process. their structures were elucidated by spectroscopic analyses. These compounds were exactly identical as those in previous studies from the other biomasses of Taxus chinensis and also other species. Also the concentrations of these compounds were compared with the concentration of the paclitaxel in various batches of plant cell cultures. As paclitaxel concentration increased at the end of cell cultures. the concentrations of the other paclitaxel derivatives decreased. The profile of these taxoids production can provide information for better understanding of structure-activity relationships and biosynthesis Importantly it can be utilized as an useful parameter for the quality control of paclitaxel production.

• 한국신재생에너지학회:학술대회논문집
• /
• 2008.05a
• /
• pp.202-205
• /
• 2008

Anaerobic digestion(AD) is the most promising method of treating and recycling of different organic wastes, such as OFMSW, household wastes, animal manure, agro-industrial wastes, industrial organic wastes and sewage sludge. During AD, i.e. degradation in the absence of oxygen, organic material is decomposed by anaerobes forming degestates such as an excellent fertilizer and biogas, a mixture of carbon dioxide and methane. AD has been one of the leading technologies that can make a large contribution to producing renewable energy and to reducing $CO_2$ and other GHG emission, it is becoming a key method for both waste treatment and recovery of a renewable fuel and other valuable co-products. A classification of the basic AD technologies for the production of biogas can be made according to the dry matter of biowaste and digestion temperature, which divide the AD process in wet and dry, mesophilic and thermophilic. The biogas produced from AD plant can be utilized as an alternative energy source, for lighting and cooking in case of small-scale, for CHP and vehicle fuel or fuel in industrials in case of large-scale. This paper provides an overview of the status of biogas production and utilization technologies.

• Progress in Superconductivity and Cryogenics
• /
• v.18 no.3
• /
• pp.1-5
• /
• 2016

Ion-beam assisted deposition (IBAD) technology has been successfully applied to high-temperature superconductor coated conductors (CC) as textured substrates. Since the coated conductors were proposed as a potential framework for utilizing the superior transport characteristics of $YBa_2Cu_3O_7$ and related cuprate oxides, several methods including rolling-assisted bi-axial textured substrates (RABiTS) and inclined substrate deposition (ISD), as well as IBAD, have been attempted. As of 2016, most companies that are trying to commercialize CC adapt IBAD technology except for American Superconductors who use RABiTS predominantly. For the materials in the IBAD process, initial efforts to use yttria-stabilized zirconia (YSZ) or related fluorites in Fujikura in Japan have quickly given way to MgO which technique was developed by Stanford University in the USA. In this review, we present a historical overview of IBAD technology, in particular, for the application of CC. We describe the key scientific understanding of nucleation, the texturing mechanism, and the growth of large bi-axial grains and discuss some potential new IBAD materials and systems for large-scale production.

• Journal of the Korean Society for Precision Engineering
• /
• v.23 no.8 s.185
• /
• pp.178-184
• /
• 2006

Hot embossing is to fabricate desired pattern on the polymer substrate by pressing the patterned mold against the substrate which is heated above the glass transition temperature, and it is a high throughput fabrication method for bio chip, optical microstructure, etc. due to the simultaneous large area patterning. However, the bad pattern fidelity in large area patterning is one of the obstacles to applying the hot embossing technology for mass production. In the present study, PDMS pad was used as a cushion on the backside of the micro-patterned 4 inch Si mold to improve the pattern fidelity over the 4 inch PMMA sheet by increasing the conformal contact between the Si mold and the PMMA sheet. The pattern replicability improvement over 4 inch wafer scale was evaluated by comparing the replicated pattern height and depth for PDMS-cushioned Si mold against the rigid Si mold without PDMS cushion.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
• /
• v.13 no.1
• /
• pp.77-83
• /
• 1995

This paper is concentrated on map generalization in digital environment for automated multi-scale map pro-duction using conventional hardcopy maps. Line generalization is urgently required process to prepare small scale digital map database when large scale map databases are available. This paper outlines a new approach to the line generalization when preparing small scale map on the basis of existing large scale distal map. Line generalizations are conducted based on zero-crossing algorithm using six sheets of 115,000 scale YEOSU area which produced by National Geographic Institute. The results are compared to Douglas-Peucker algorithm and manual method. The study gives full details of the data reduction rates and alternatives based on the proposed algorithm.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.7
• /
• pp.1093-1100
• /
• 2015

Acetate and lactate in growth media are detrimental to the production of Thermus maltogenic amylase (ThMA), a heterologous protein, as well as to the growth of recombinant Escherichia coli. Only 50 mM of acetate or 10 mM of lactate reduced 90% of specific ThMA activity. In this study, mutant E. coli strains blocked in the ackA-pta or ackA-pta and ldh pathways were created, characterized, and assessed for their culture performace in 300 L-scale fermentation. The ackApta and ldh double-mutant strain formed significantly less lactate and acetate, and produced a concomitant increase in the excretion of pyruvate (17.8 mM) under anaerobic conditions. The ackA-pta mutant strain accumulated significant acetate but had an approximately 2-fold increase in the formation of lactate. The ackA-pta and ldh double-mutant strain had superior overall performance in large-scale culture under suboptimal conditions, giving 67% higher cell density and 66% higher ThMA activity compared with those of the control strain. The doublemutant strain also achieved a 179% improvement in volumetric ThMA production.

• Journal of Veterinary Clinics
• /
• v.16 no.1
• /
• pp.163-176
• /
• 1999

The nuclear transplantation technique is known as the most potential and efficient method for producing large numbers of genetically identical animals from a single embryo and somatic cells. After Dolly was introduced in 1997, many scientists were amazed. A possibility came to a reality that live offspring could be produced with differentiated somatic cells from an adult animal. On the other side, many in the press and the sensationalists focused on the socially, ethically and scientifically unacceptable sides of the technology. In this article, the history, current status and prospects of the technological development of nuclear transplantation in mammals and its application to the production of cloned animals are described. For the efficient and successful production of cloned embryos by nuclear transplantation, the right selection, preactivation and micromanipulation of oocytes as capacious recipient cytoplasm, the adequate and benefitial preparation of multiple totipotent embryonic and somatic cells as donor nuclei, fusion of them and in vitro production of cloned embryos are very critical. Recently the overall efficiency of production of cloned embryos and offspring in livestock has been much improved. Cloning will also be a more efficient, faster and useful way of creating transgenic fetuses for gene therapies, gene pharming, organs for xenotransplantation by preselection and mass production of transgenic embryos and consequently improving the production efficiency in transgenic animals. Further technical development of nuclear transplantation will enable large-scale production of cloned livestock and in near future the commercial cloning of animals will become a reality.

• Proceedings of the Korea Contents Association Conference
• /
• 2008.05a
• /
• pp.198-202
• /
• 2008

Manufacturing process of large-scale 3D computer animation is becoming sophistication, ramification because of development of manufacturing technique and extravagant budget. Form of manufacturing pipeline has been variously changed to production type, manufacturing scale, manufacturing form. But it is time that renewed discussion is needed because change and development for the organization is insufficient in small manufacturing company. The project aims to try to help understanding for manufacturing pipeline of internal small-scale 3D animation and to find a plan of organization for internal small-scale production of the real situation. Organization model and methodology of manufacturing pipeline of small manufacturing company is not absolute because it is enough possible to be changed to inclination of the project and its environment. People must fully understand the purpose for organization of manufacturing pipeline of 3D computer animation and it must be organized to the situation for small-scale production so that every worker in production can share the information perfectly.

• Journal of Korean Institute of Industrial Engineers
• /
• v.29 no.4
• /
• pp.334-344
• /
• 2003

A mixture experiment is a special type of response surface experiment in which factors are the ingredients or components of a mixture, and the response is a function of the proportions of each ingredient. Evolutionary operation is useful to improve on-line full-scale manufacturing process by systematically changing the levels of the process variables without jeopardizing the product. This paper presents an evolutionary operation procedure with mixture variables for large-scale mixture production process which can be beneficial to practitioners who should improve on-line mixture quality while maintaining the production amount of the mixture product.

• Journal of Agricultural Extension & Community Development
• /
• v.24 no.1
• /
• pp.1-8
• /
• 2017

The Purpose of this study is to predict farm size per farming household in Chungcheongnam-province by using the Markov chains and Quadratic Programming.. The results are as follows; First, small-scale farms with less than 1.0ha of land are predicted to be still more than half (of total farming households) in 2025 as well. Second, large-scale farms with 3.0ha-5.0ha land and extra large-scale farms with over 5.0ha of land are predicted to gradually expand their proportion in total farm scale. Third, middle-scale farms with 1.0ha-3.0ha land are forecasted to be reduced in their relative proportion. It is required to take into account regional characteristics to improve the effectiveness of a rice industry policy. Therefore, this study has some significance in attempting to research on the ownership structure of rice production areas in consideration of target regions.