• Structural Engineering and Mechanics
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• v.54 no.2
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• pp.379-392
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• 2015

A number of acceleration-based damage detection methods have been developed but they have not been widely applied in engineering practices because the acceleration response is insensitive to minor damage of civil structures. In this article, a damage detection approach using the long-gauge strain sensing technology and the principle component analysis technology is proposed. The Long gauge FBG sensor has its special merit for damage detection by measuring the averaged strain over a long-gauge length, and it can be connected each other to make a distributed sensor network for monitoring the large-scale civil infrastructure. A new damage index is defined by performing the principle component analyses of the long-gauge strains measured from the intact and damaged structures respectively. Advantages of the long gauge sensing and the principle component analysis technologies guarantee the effectiveness for structural damage localization. Examples of a simple supported beam and a steel stringer bridge have been investigated to illustrate the successful applications of the proposed method for structural damage detection.

• Korean journal of applied entomology
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• v.34 no.3
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• pp.243-248
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• 1995

In order to isolate naturally occurring novel Bacillus thuringiensis strains, we investigated the distribution of B. thuringiensis from granary which exist in Kyong-gi province, Korea. A total of 146 strains of B. thuringiensis producing spore and crystal wre isolated. The toxicity of B. thuringiensis isolates was examined against lepidopteran larvae (Bombyx mori), dipteran larvae (Culex pipiens) and coleopteran larvae (Sitophilus oryzae.), respectively. The results showed that a large number of B. thuringiensis isolates from granary dusts were isolated and most isolates wer toxic to lepidopterous larvae. Also, non-toxic B. thuringiensis isolate was common. In order to isolate many B. thuringiensis, therefore, it suggested that granary is favorable locality.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.11 no.3
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• pp.1-10
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• 1991

The paper is concerned with the analysis of laminated composite shell structures using an improved degenerated shell element. In the formulation of the element stiffness, the combined use of three different techniques was made. They are; 1) an enhanced interpolation of transverse shear strains in the natural coordinate system to overcome the shear locking problem; 2) the reduced integration technique in in-plane strains to avoid the membrane locking behavior; and 3) selective addition of the nonconforming displacement modes to improve the element performances. This element is free of serious shear/membrane locking problems and undesirable compatible/commutable spurious kinematic deformation modes. An incremental total Lagrangian formulation is presented which allows the calculation of arbitrarily large displacements. The resulting non-linear equilibrium equations are solved by the Newton-Raphson method. The versatility and accuracy of this improved degenerated shell element are demonstrated by solving several numerical examples.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.81-85
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• 2002

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Stenotrophomonas, but were not included in these genera. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium. We attempted to screening of bioactive compounds from oligotrophs which was isolated from forest soil. The active compounds were analyzed by mass and NMR spectrum, one of them identified as crisamicin A. Another one designated as SAPH is a new compound. The results indicate that there were possibilities for finding new compounds from the rare microorganisms such as oligotrophs.

• Journal of Microbiology and Biotechnology
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• v.22 no.6
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• pp.849-855
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• 2012

Plant growth-promoting rhizobacteria (PGPR) pseudomonads have a large number of lipopolysaccharides on the cell surface, which induces immune responses. Cd-resistant PGPR prevalent at the Cd-affected sites under biophytostabilization was monitored. Transmissiom electron microscopy was used to the study the behavior of tolerance of PGPR to cadmium level and its effect on pseudomonad strains (Z9, S2, KNP2, CRPF, and NBRI). An immunosensor was developed by immobilizing antibody (anti-Z9 or anti-S2) against selected PGPR on a piezoelectric quartz crystal microbalance (QCM). Immunosensors were found to supplement the inherent specificity of antigen-antibody reactions with the high sensitivity of a physical transducer. On comparison of the efficiency of detection with ELISA, the spectrophotometric technique, the developed immunosensor was found to be more sensitive, fast, and reliable even after regeneration for several times. Thus, the immunosensor may be used for future detection of PGPR strains after automation of the screening process.

• Journal of Microbiology and Biotechnology
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• v.23 no.7
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• pp.885-896
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• 2013

2,3-Butanediol (2,3-BDO) has immense industrial applications. Recently, microbial fermentation has emerged as an alternative way to produce this industrially important chemical. Although 2,3-BDO is produced by several microorganisms, the Klebsiella genera has an excellent production compared with other 2,3-BDO-producing microorganisms. In order to produce 2,3-BDO on a large scale, the challenges of removing pathogenic factors from Klebsiella pneumoniae need to be addressed. K. pneumoniae produces a number of virulence factors that contribute to its pathogenesis, including lipopolysaccharides, capsules, fimbrial adhesins, etc. Removal of these pathogenic factors from 2,3-BDO-producing Klebsiella strains will result in avirulent strains for the safe, economic, and efficient production of 2,3-BDO. In this review, we summarize the current trends in 2,3-BDO production using K. pneumoniae and insights into the removal of its virulence factors for industrial applications.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.6
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• pp.659-665
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• 2010

The finite element method(FEM) is proven to be an effective approximate method of structural analysis if proper element types and meshes are chosen, and recently, the method is often applied to solve complex dynamic and nonlinear problems. A properly chosen element type and mesh yields reliable results for dynamic finite element structural analysis. However, dynamic behavior of a structure may include unpredictably large strains in some parts of the structure, and using the initial mesh throughout the duration of a dynamic analysis may include some elements to go through strains beyond the elements' reliable limits. Thus, the finite element mesh for a dynamic analysis must be dynamically adaptive, and considering the rapid process of analysis in real time, the dynamically adaptive finite element mesh generating schemes must be computationally efficient. In this paper, a computationally efficient dynamically adaptive finite element mesh generation scheme for dynamic analyses of structures is described. The concept of representative strain value is used for error estimates and the refinements of meshes use combinations of the h-method(node movement) and the r-method(element division). The shape coefficient for element mesh is used to correct overly distorted elements. The validity of the scheme is shown through a cantilever beam example under a concentrated load with varying values. The example shows reasonable accuracy and efficient computing time. Furthermore, the study shows the potential for the scheme's effective use in complex structural dynamic problems such as those under seismic or erratic wind loads.

• Mycobiology
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• v.48 no.2
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• pp.104-114
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• 2020

The carbohydrate-active enzyme (CAZyme) genes of Trametes contribute to polysaccharide degradation. However, the comprehensive analysis of the composition of CAZymes and the biosynthetic gene clusters (BGCs) of Trametes remain unclear. Here, we conducted comparative analysis, detected the CAZyme genes, and predicted the BGCs for nine Trametes strains. Among the 82,053 homologous clusters obtained for Trametes, we identified 8518 core genes, 60,441 accessory genes, and 13,094 specific genes. A large proportion of CAZyme genes were cataloged into glycoside hydrolases, glycosyltransferases, and carbohydrate esterases. The predicted BGCs of Trametes were divided into six strategies, and the nine Trametes strains harbored 47.78 BGCs on average. Our study revealed that Trametes exhibits an open pan-genome structure. These findings provide insights into the genetic diversity and explored the synthetic biology of secondary metabolite production for Trametes.

• Microbiology and Biotechnology Letters
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• v.48 no.2
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• pp.121-128
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• 2020

Diarrhea is a major public health concern associated with pathogenic Escherichia coli infections. Food-borne pathogenic E. coli can lead to large diarrheal outbreaks and hence, there is a need to estimate the frequency of pathogenic E. coli load in the various types of meat available in markets. In the present study, we classified and characterized diarrheagenic E. coli isolates collected from 399 raw meat samples from retail sources in Korea. Shiga toxin-producing E. coli (STEC) were detected in 11 (9.7%) samples, including nine strains (8.0%) in beef and two strains (1.8%) in chicken. The frequency of the detected virulence markers were as follows: astA, 28.3%; escV,18.6%; eaeA,17.7%; ent, 7.0%; EHEC-hly, 4.4%; stx1, 3.5%; and stx2, 3.5%. We did not observe any typical EPEC, EIEC, or ETEC virulence determinants in any of the samples. The STEC serotype O26 was detected in one sample, but no other serogroups (O91, O103, O128, O157, O145, O111, and O121) were found. Further research is needed to better understand the virulence mechanism of STEC serotypes, their ecology, and prevalence in animals, food, and the environment. These results will help improve risk assessment and predict the sources of food poisoning outbreaks.

• ALGAE
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• v.28 no.4
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• pp.307-330
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• 2013

The genus Cryptomonas is easily recognized by having two flagella, green brownish color, and a swaying behavior. They have relatively simple morphology, and limited diagnostic characters, which present a major difficulty in differentiating between species of the genus. To understand species delineation and phylogenetic relationships among Cryptomonas species, the nuclear-encoded internal transcribed spacer 2 (ITS2), partial large subunit (LSU) and small subunit ribosomal DNA (rDNA), and chloroplast-encoded psbA and LSU rDNA sequences were determined and used for phylogenetic analyses, using Bayesian and maximum likelihood methods. In addition, nuclear-encoded ITS2 sequences were predicted to secondary structures, and were used to determine nine species and four unidentified species from 47 strains. Sequences of helix I, II, and IIIb in ITS2 secondary structure were very useful for the identification of Cryptomonas species. However, the helix IV was the most variable region across species in alignment. The phylogenetic tree showed that fourteen species were monophyletic. However, some strains of C. obovata had chloroplasts with pyrenoid while others were without pyrenoid, which used as a key character in few species. Therefore, classification systems depending solely on morphological characters are inadequate, and require the use of molecular data.