• 한국자동차공학회논문집
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• 제4권2호
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• pp.80-89
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• 1996

Fatigue life of a notched shaft was evaluated in order to estimate the durability and integrity of the notched shaft in design stage. Cumulative fatigue dama- ge analysis was performed using local strain approach based on the assumption that the fatigue life of a notched component is approximately same as that of a smooth specimen is subjected to the same strain at the notched component. In this paper, shafts with different notch root radius of 1, 2㎜ resulting in different values of stress concentration factors were tested under||rotating bending fatigue loading condition. Theoretical stress concentration factor for each notch type was calculated using finite element method. Fatigue life prediction program, FALIPS, written in C language was developed using the strain-life curve, and the local strain approach integrating Neuber's rule, cyclic stress-strain, and hysteresis loop equations. The fatigue life evaluated using the fatigue notch factor obtained from the experimentally determined fatigue strength showed very large scattering with nonconservatism, but the fatigue notch factors derived from the stress concentration factors and Peterson's equation reduced the considerablely accurate fatigue life evaluation within a factor of three.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2006년도 정기 학술대회 논문집
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• pp.159-166
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• 2006

Although the strain distribution along the length of a beam in buildings or infrastructures is non-uniform, most fiber optic sensors are point sensors that can measure the strain only at a local point of a beam. Long gage fiber optic sensors that measure integrated strain over a relatively long length can consider strain variation. This type of sensor was found to be efficient and useful for monitoring large-scale structures. On the other hand, the maximum strain or stress in a beam can not be measured with long gage optic sensors. However, for the assessment of the safety of multi-span steel beams subjected to various vertical loads, the maximum strain or stress measured during monitoring is required for comparison with the allowable stress of the beam calculated by a design code. Therefore, in this paper, mathematical models are presented for determination of the maximum values of strains in more three-span steel beams based on the average strains measured by long gage optic sensors.

• Structural Engineering and Mechanics
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• 제74권3호
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• pp.351-363
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• 2020

Based on the dynamic tests of recycled aggregate concrete (RAC) short columns confined by the hoop reinforcement, the dynamic failure mechanism and the mechanical parameters related to the constitutive relation of confined recycled aggregate concrete (CRAC) were investigated thoroughly. The fracturing sections were relatively flat and smooth at higher strain rates rather than those at a quasi-static strain rate. With the increasing stirrup volume ratio, the crack mode is transited from splitting crack to slipping crack constrained with large transverse confinement. The compressive peak stress, peak strain, and ultimate strain increase with the increase of stirrup volume ratio, as well as the increasing strain rate. The dynamic confining increase factors of the compressive peak stress, peak strain, and ultimate strain increase by about 33%, 39%, and 103% when the volume ratio of hoop reinforcement is increased from 0 to 2%, but decrease by about 3.7%, 4.2%, and 9.1% when the stirrup spacing is increased from 20mm to 60mm, respectively. This sentence is rephrased as follows: When the stirrup volume ratios are up to 0.675%, and 2%, the contributions of the hoop confinement effect to the dynamic confining increase factors of the compressive peak strain and the compressive peak stress are greater than those of the strain rate effect, respectively. The dynamic confining increase factor (DCIF) models of the compressive peak stress, peak strain, and ultimate strain of CRAC are proposed in the paper. Through the confinement of the hoop reinforcement, the ductility of RAC, which is generally slightly lower than that of NAC, is significantly improved.

• 한국지반공학회논문집
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• 제34권11호
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• pp.81-92
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• 2018

최근 해상 교량 기초 시공 중 차수 및 연직 하중을 지지하는 역할을 수행하는 대형 원형 강관 가설 공법이 제안되었다. 하지만, 대형 원형 강관의 시공 및 운용 중에 발생할 수 있는 선박 충돌과 같은 위부 요인은 구조물의 안정성에 악영향을 줄 수 있다. 따라서, 본 연구에서는 외부 충격에 의한 대형 원형 강관의 동적 반응을 평가할 수 있는 기법을 개발하기 위해 실내 실험을 통한 기초 연구를 수행하였다. 실내 실험에서는 해상에 설치된 대형 원형 강관을 모사하기 위하여 소형 모형 강관을 토조에 설치하였고, 토조 속 흙의 높이와 수위는 각각 23cm와 25cm로 설정하였다. 수위는 40cm, 55cm, 70cm로 변화시켜가며 실험을 수행하였다. 선박 충돌을 모사하기 위하여 모형 강관의 상부를 해머로 타격하였으며, 모형 강관의 길이 방향으로 설치된 변형률계와 상부에 설치된 가속도계로 신호를 측정하였다. 실험결과, 변형률계로 측정된 변형률이 모형 강관의 상부에서 하부로 내려갈수록 감소하였다. 변형률계로 측정된 신호의 주파수는 충격이 가해지면 상시미진동 주파수보다 크게 증가하였지만, 수위 아래에 위치한 변형률계에서 측정된 주파수는 큰 증가를 나타내지 않았다. 가속도계로 측정된 신호의 최대 주파수는 충격이 가해지면 상시미진동 주파수보다 크게 증가하였다. 수위가 증가하면 최대 주파수는 감소하지만, 상시미진동 주파수보다는 크게 나타났다. 본 연구의 결과는 변형률계와 가속도계가 대형 원형 강관의 동적 반응 특성을 평가하는데 유용한 지표가 될 수 있음을 보여준다.

• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.1093-1100
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• 2015

Acetate and lactate in growth media are detrimental to the production of Thermus maltogenic amylase (ThMA), a heterologous protein, as well as to the growth of recombinant Escherichia coli. Only 50 mM of acetate or 10 mM of lactate reduced 90% of specific ThMA activity. In this study, mutant E. coli strains blocked in the ackA-pta or ackA-pta and ldh pathways were created, characterized, and assessed for their culture performace in 300 L-scale fermentation. The ackApta and ldh double-mutant strain formed significantly less lactate and acetate, and produced a concomitant increase in the excretion of pyruvate (17.8 mM) under anaerobic conditions. The ackA-pta mutant strain accumulated significant acetate but had an approximately 2-fold increase in the formation of lactate. The ackA-pta and ldh double-mutant strain had superior overall performance in large-scale culture under suboptimal conditions, giving 67% higher cell density and 66% higher ThMA activity compared with those of the control strain. The doublemutant strain also achieved a 179% improvement in volumetric ThMA production.

• 미생물학회지
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• 제15권3호
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• pp.103-112
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• 1977

Several strains of spore-forming lacticacid bacteria were isolated from natural sources such as soils, cereals, and foods. The general morphological and physiological characteristics of the strain 6-4 were investigated nad compared with some other industrial strains. The effects of fructose-1,6-diphoshpate (FDP), adenosine triphosphate (ATP), and pH on the lactate dehydrogenase(LDH) activity of the strain were studied, and the changes in LDH activity and spore formation under various cultural conditions were researched. The results were as follows. 1. This strain was identified to Bacillus coagulans Hammer and distributed widely in natural sources. 2. The strain strongly converted various fermentation substrates in to L(+)-lacticacid in anaerobic conditioins, and many spores that were of great advantages to the industrial application were formed easily in the aerobic condition. 3. The LDH activity of this strain was activated by FDP and inhibited by ATP. The optimal pH for the enzyme activity was 6.0-6.5. 4. In the anaerobic culture condifion, the large amount of glucose added in the medium increased the LDH activity, but the cells were not committed to sporulate. 5. When none or a very small amount of glucose (less than 0.5%) was added to culture medium in the aerobic condition, the LDH activity was decreased and many spore were produced with final pH higher than 8.5. 6. The additioin of large amount of glucose (more than 2.0%) in aerobic culture increased the LDH activity and inhibited strongly the spore formation with final pH lower than 6.0.

• 한국재료학회지
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• 제21권11호
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• pp.628-633
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• 2011

Studies on lead-free piezoelectrics have been attractive as means of meeting environmental requirements. We synthesized lead-free piezoelectric $(Bi_{1/2}Na_{1/2})TiO_3-Ba(Cu_{1/3}Nb_{2/3})O_3$ (BNT-BCN) ceramics, and their dielectric, piezoelectric, and strain behavior were characterized. As BCN with a tetragonal phase was incorporated into the rhombohedral BNT lattice, the lattice constant increased. A small amount of BCN increased the density and dielectric constant forming the complete solid solution with BNT. However, BCN above 10 mol% was precipitated into a separate phase, and which was detected with XRD. In addition, EDX measurement revealed that Cu in BCN was not distributed homogeneously but was accumulated in a certain area. A lower density with a large amount of BCN was attributed to the nonsinterable property of BCN with large tetragonaliy. The dielectric constant vs the temperature change and the strain vs the electric field indicated that the ferroelectric property of BNT was diminished and paraelectric behavior was enhanced with the BCN addition. BNT-7.5BCN showed a 0.11% unimorph strain with a 9.0 kV/mm electric field with little hysteresis.

• Mycobiology
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• 제48권6호
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• pp.450-463
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• 2020

The strains 17E-042, 17E-039, and NC13-171 belong to Ascomycota and were isolated from soil collected from Sancheong-gun and Yeongam-gun, Korea. The strain 17E-042 produced white mycelial colonies that developed a sienna color with a round margin on potato dextrose agar (PDA), and the reverse side developed a light sienna color. Morphologically, this strain was similar to the strains of Arthrinium phragmites and A. hydei, but the shorter conidial size of the newly identified strain (17E-042) was distinct. The strain 17E-039 produced macroconidia that were pale yellow to orange-brown, elongated-ellipsoid to oblong, round at both ends, primarily straight but sometimes slightly curved, 0-septate, thin-walled, and filled with numerous droplets, having diameters of 20.4-34.3 × 8.0-12.0 ㎛. And the strain NC13-171 formed hyaline to light brown chlamydospores, solitary or in a chain. Multigene phylogenetic analyses were conducted using sequence data obtained from internal transcribed spacer (ITS) regions, 28S rDNA large subunit (LSU), β-tubulin (TUB2), translation elongation factor 1-alpha (TEF1-α), and RNA polymerase II large subunit (RPB2) genes. The results of molecular phylogeny, the detailed descriptions and illustrations of each species strongly support our proposal that these strains from soil in Korea be designated as Arthrinium minutisporum sp. nov. and two new records of Pezicula neosporulosa and Acrocalymma pterocarpi.

• Mycobiology
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• 제51권3호
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• pp.115-121
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• 2023

The fungal strain KNUF-22-18B, belonging to Cucurbitariaceae, was discovered from a stink bug (Hygia lativentris) during the investigation of insect microbiota in Chungnam Province, South Korea. The colonies of the strain KNUF-22-18B were wooly floccose, white to brown in the center on oatmeal agar (OA), and the colonies were buff, margin even, and colorless, reverse white to yellowish toward the center on malt extract agar (MEA). The strain KNUF-22-18B produced pycnidia after 60 days of culturing on potato dextrose agar, but pycnidia were not observed on OA. On the contrary, N. keratinophila CBS 121759^T abundantly formed superficial pycnidia on OA and MEA after a few days. The strain KNUF-22-18B produced chlamydospores subglobose to globose, mainly in the chain, with a small diameter of 4.4-8.8 ㎛. At the same time, N. keratinophila CBS 121759^T displayed a globose terminal with a diameter of 8-10 ㎛. A multilocus phylogeny using the internal transcribed spacer regions, 28S rDNA large subunit, b-tubulin, and RNA polymerase II large subunit genes further validated the uniqueness of the strain. The detailed description and illustration of the proposed species as Neocucurbitaria chlamydospora sp. nov. from Korea was strongly supported by molecular phylogeny.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2002년도 학술발표회 발표논문집
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• pp.329-332
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• 2002

In the present study, in order to apply a cyclic viscoelastic-viscoplastic constitutive model to multi-layered ground conditions during large earthquake, the numerical simulations of the 1995 Hyogoken Nanbu Earthquake at Port Island, Kobe, Japan, were performed by the seismic respons analysis. From the seismic response analysis, it was verified that a cyclic viscoelastic-viscoplastic constitutive model can give a good description of the damping characteristics of clay accurately during large event which induces plastic deformation in large strain range.