• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.129-133
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• 1997

A total 943 accession of soybeans (G. max) and 50 wild soybeans (G. soja) were examined for leucine aminopeptidase (LAP) isozyme variation by 5％ polyacrylamide gel electrophoresis(PAGE) and isoelectric focusing(IEF) of pH 4~6.5. The Lap1*b by PAGE was the most common phenotype in both G. max and G. soja. The frequency of Lap1*b allele was observed to be higher in G. max(1.00) than in G. soja(0.96) of Korea. This result shows that G. max is fixed for Lapl*b allele at the Lap1 locus. LAP isozyme band type I and II were found using IEF of pH 4~6.5 in G. max and G. soja of Korea. Type I was observed from 92.8％ in G. max and 92.0％ in G. soja, and type II was discovered in 7.2％ G. max and 8.0％ G. soja. This result suggested the possibility to be found more various band types.

• Journal of Korean Society of Forest Science
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• v.43 no.1
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• pp.39-50
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• 1979

Twelve Pinus taeda were used as mother trees and one P. rigida plus tree was used as pollen tree for 12 cross combinations. Nine P. densiflora plus trees(4 mother and 7 pollen trees) were used as parents for 11 cross combinations. Those parents and 10 progenies were analyzed for LAP of ${\times}$P. taeda rigida hybrid and P. densiflora and for peroxidase of ${\times}$P. taeda rigida. The analysis, based on the banding patterns, indicate three alleles for LAP-A locus(A1, A2, A3) and two alleles for LAP-B locus (B1, B2) in ${\times}$P. taeda rigida hybrids. Chi-square test on the segregation for progenies did not show significant differences. The results indicated good agreement with monohybrid Mendelian inheritance. Independence test for occurrence frequency of 2 alleles(LAP-A3, LAP-B2) illustrated that there is neither linkage nor repulsion relationship between LAP-A3 and LAP-B2 alleles. Three band at LAP-A locus were always exhibited from all parents and their progenies of P. densiflora. However, the occurrence of two bands at LAP-B locus was variable, one bands assumed as homozygous alleles(B2/B2) and two bands as heterozygous alleles(B1/B2). The segregation ratio for progenies of P. densiflora suggested that LAP-B locus may be controlled by two alleles(B1 and B2). Three Peroxidase loci(Px-A, Px-B, Px-C) assumed to be controlled by allozyme in ${\times}$P. taeda rigida hybrid. The Px-B and Px-C loci could not find out the variations from banding patterns of parents and their progenies, while the Px-A locus showed the variations of occurrence frequency by two bands. The segregation ratio for A1/A2 at LAP-A locus suggest that the peroxidase allozymes of ${\times}$P. taeda rigida hybrid appeare to be monomeric products; that is, Px-A locus may be controlled by two alleles (A1 and A2).

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.1
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• pp.9-13
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• 2013

The aim of the present study was to examine the effect of micellar systems on the absorption of beta-lapachone (b-lap) through different intestinal segments using a single-pass rat intestinal perfusion technique. B-lap was solubilized in mixed micelles composed of phosphatidylcholine and sodium deoxycholate, and in sodium lauryl sulfate (SLS)-based conventional micelles. Both mixed micelles and SLS micelles improved the in situ permeability of b-lap in all intestinal segments tested although the mixed micellar formulation was more effective in increasing the intestinal absorption of b-lap. The permeability of b-lap was greatest in the large intestinal segments. Compared with SLS micelles, the effective permeability coefficient values measured with mixed micelles were 5- to 23-fold higher depending on the intestinal segment. Our data suggest that b-lap should be delivered to the large intestine using a mixed micellar system for improved absorption.

• Journal of the Korea Convergence Society
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• v.10 no.2
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• pp.301-307
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• 2019

The purpose of this study is to evaluate the lap time of K-1 sprint competition and compare the records of the best group and the other groups. Based on these data we are willing to provide them on pace control strategies for improving their performances. The analysis was conducted on 12 elite kayak players, measuring the lap time by every interval after dividing the entire 1,000-meter race into 10 sections of 100 meters. We divide the group A, B, C and D based on their final time records. Two-factor repeated measures ANOVA, One-way ANOVA, and correlation analysis were performed. There were no significant lap time differences in the section of first 200m and last 200m between A, B and C. But the C's lap time of 200m~600m were significantly later than group A and B. Non-superior athletes need to establish strategies to reduce lap time in the middle section.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.120-123
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• 2004

The lap splice lengths of deformed steel reinforcing bars and GFRP bars were experimentally compared using beam specimens. The purpose was to evaluate the length required of the GFRP bar to develop strength at least equivalent to the conventional steel reinforcing bar. The main test variable was the lap splice length: 10, 20, 30 $d_b$ for the deformed steel bars and 20, 30, 40 $d_b$ for the GFRP bars. Two different types of GFRP bars were tested: (1) one with spiral-type deformation and (2) plain round bars. Elastic modulus was about 1/5 of the steel bars while the tensile strength was about 690 MPa for the GFRP bars. Nominal diameter of the GFRP bars and steel bars was 12.7 and 13 mm, respectively. Normal strength concrete (28-day $f_{cu}$ = 30 MPa) was used. For the conventional steel bars (SD400 grade), strength over 400 MPa in tension was developed using the lap splice length of 20 and 30 $f_{cu}$. Only $87\%$ of the nominal yield strength was reached with the lap splice length of 10 $d_b$. For the spiral-type deformed GFRP bars with $40-d_b$ lap splice length, 440 MPa in tension was determined. The maximum tensile strength developed of the GFRP bars with smaller lap splice lengths decreased. The plain GFRP bar was not effective in developing the tensile strength even with $40-d_b$ lap splice length. Development of the cracks on beam surface was clearly visible for the beams reinforced with the GFRP bars. Mid-span deflections, however, were significantly smaller than the comparable beams with conventional steel bars indicating potential ductility problem.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.6
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• pp.262-269
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• 2021

This paper conducts an evaluation of the structural performance of the lap splice detail of SD700 headed bar experiment for developing an RC beam with different depths joint details. The experiment variable is lap splice length, yield strength, and end anchorage of main reinforcements. For all specimens, a headed bar was applied to the main reinforcement of the beam with low depth (B2), and the beam with high depth (B1) was applied to the main reinforcement with two splice methods: straight headed bar and 90° hooked-headed bar. The experimental results were that specimens of applying SD500 and SD600 had the results of flexural fracture at the lap splice location, which maximum load was similar. For specimens of appling SD500, the 90° hooked-headed bar of B1, suppressed horizontal cracks in the lap splice section compared to the straight headed bar. Specimens of applying an SD 700 headed bar had the results of brittle anchorage failure. In addition, maximum load was increased with the lap splice length increasing. For specimens of applying SD700 headed bar, test for test maximum load/theoretical load for test development length/design development length were estimated to be 1.30~1.48 for the ACI 318-19 equation, and 1.14~1.30 for the KDS-2021 equation. Thus, ACI 318-19 equation had conservatively greater safety factors as estimated development lengththened.

• Radiation Oncology Journal
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• v.31 no.2
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• pp.57-65
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• 2013

Beta-lapachone (${\beta}$-Lap; 3,4-dihydro-2, 2-dimethyl-2H-naphthol[1, 2-b]pyran-5,6-dione) is a novel anti-cancer drug under phase I/II clinical trials. ${\beta}$-Lap has been demonstrated to cause apoptotic and necrotic death in a variety of human cancer cells in vitro and in vivo. The mechanisms underlying the ${\beta}$-Lap toxicity against cancer cells has been controversial. The most recent view is that ${\beta}$-Lap, which is a quinone compound, undergoes two-electron reduction to hydroquinone form utilizing NAD(P)H or NADH as electron source. This two-electron reduction of ${\beta}$-Lap is mediated by NAD(P)H:quinone oxidoreductase (NQO1), which is known to mediate the reduction of many quinone compounds. The hydroquinone forms of ${\beta}$-Lap then spontaneously oxidizes back to the original oxidized ${\beta}$-Lap, creating futile cycling between the oxidized and reduced forms of ${\beta}$-Lap. It is proposed that the futile recycling between oxidized and reduced forms of ${\beta}$-Lap leads to two distinct cell death pathways. First one is that the two-electron reduced ${\beta}$-Lap is converted first to one-electron reduced ${\beta}$-Lap, i.e., semiquinone ${\beta}$-Lap $(SQ)^{{\cdot}-}$ causing production of reactive oxygen species (ROS), which then causes apoptotic cell death. The second mechanism is that severe depletion of NAD(P)H and NADH as a result of futile cycling between the quinone and hydroquinone forms of ${\beta}$-Lap causes severe disturbance in cellular metabolism leading to apoptosis and necrosis. The relative importance of the aforementioned two mechanisms, i.e., generation of ROS or depletion of NAD(P)H/NADH, may vary depending on cell type and environment. Importantly, the NQO1 level in cancer cells has been found to be higher than that in normal cells indicating that ${\beta}$-Lap may be preferentially toxic to cancer cells relative to non-cancer cells. The cellular level of NQO1 has been found to be significantly increased by divergent physical and chemical stresses including ionizing radiation. Recent reports clearly demonstrated that ${\beta}$-Lap and ionizing radiation kill cancer cells in a synergistic manner. Indications are that irradiation of cancer cells causes long-lasting elevation of NQO1, thereby sensitizing the cells to ${\beta}$-Lap. In addition, ${\beta}$-Lap has been shown to inhibit the repair of sublethal radiation damage. Treating experimental tumors growing in the legs of mice with irradiation and intraperitoneal injection of ${\beta}$-Lap suppressed the growth of the tumors in a manner more than additive. Collectively, ${\beta}$-Lap is a potentially useful anti-cancer drug, particularly in combination with radiotherapy.

• Biomolecules & Therapeutics
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• v.32 no.5
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• pp.531-539
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• 2024

Sleep is one of the most essential physiological phenomena for maintaining health. Sleep disturbances, such as insomnia, are often accompanied by psychiatric or physical conditions such as impaired attention, anxiety, and stress. Medication used to treat insomnia have concerns about potential side effects with long-term use, so interest in the use of alternative medicine is increasing. In this study, we investigated the hypnotic effects of β-lapachone (β-Lap), a natural naphthoquinone compound, using pentobarbital-induced sleep test, immunohistochemistry, real-time PCR, and western blot in mice. Our results indicated that β-Lap exerts a significant hypnotic effect by showing a decrease in sleep onset latency and an increase in total sleep time in pentobarbital-induced sleep model. The results of c-Fos immunostaining showed that β-Lap decreased neuronal activity in the basal forebrain and lateral hypothalamus, which are wakefulness-promoting brain regions, while increasing in the ventrolateral preoptic nucleus, a sleep-promoting region; all these effects were significantly abolished by 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), an adenosine A₁ receptor (A₁R) antagonist. Western blot analysis showed that β-Lap increased extracellular signal-regulated kinase phosphorylation and nuclear factor-kappa B translocation from the cytoplasm to the nucleus; these effects were inhibited by DPCPX. Additionally, β-Lap increased the mRNA levels of A₁R. Taken together, these results suggest that β-Lap exerts hypnotic effects, potentially through A₁R.

• Journal of Korean Society of Forest Science
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• v.58 no.1
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• pp.1-7
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• 1982

Megagametophyte and embryo tissue of Pinus densiflora were subjected to study the inheritance of glutamate-oxalate transaminase(GOT) and leucine aminopeptidase(LAP), and linkage relationship among isozyme loci coding both enzymes by starch gel zone-electrophoresis. Four zones of activity were observed for GOT. No variation was found in the fastest migrating zone (GOT-A). Electrophoretic phenotypes of the other two zones (GOT-B and GOT-C) showed 1:1 segregation ration, suggesting that each zone is controlled by a single locus. Foru and three alleles were identified at both loci respectively. The isozyme pattern of the fourth zone(GOT-D), migrated cathodally, coincided precisely with that of GOT-C. Whether the two zones are controlled by the same locus or by two tightly linked loci remained unknown. In all three variant GOT zones, heterozygoes embryos produced triple band patterns, indicating that GOT isozyme in Pinus densiflora is a dimer. Two zones of activity stained for LAP were found. The segregation of the two zones (LAP-A and LAP-B) suggested that tow loci control each of both isozymes. Two and three alleles were identified at both loci. GOT-B and LAP-B were found to be tightly linked, showing an average recombination frequency of 12.5 percent. Slight deviation from independent assortment was observed between GOT-B and GOT-C, with recombination frequency of 41 percent.

• Laser Solutions
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• v.15 no.1
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• pp.1-5
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• 2012

Recently ultra high strength steels(UHSS) has been widely applied to the structural or safety components in the automotive industry. Specially, hot stamping boron steel 22MnB5 has shown the crash-resistant characteristics when applying to bumpers and pillars. Lap joint Laser welding of the hot stamped and die quenched sheets of Boron steel was carried out using 3kW Nd/YAG laser. The appropriate Lap joint laser welding conditions were founded separately for four lap joint combinations. The lower sheest is a hot stamped sheet in common and the upper sheet is selected among the hot stamped steel and high strength steels such as SPCC, 370MPa, and 590MPa grade high strength steels. Cross bead sections and local hardening and softening were observed as well as tensile-shear test results.