• Laser Solutions
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• v.15 no.1
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• pp.1-5
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• 2012

Recently ultra high strength steels(UHSS) has been widely applied to the structural or safety components in the automotive industry. Specially, hot stamping boron steel 22MnB5 has shown the crash-resistant characteristics when applying to bumpers and pillars. Lap joint Laser welding of the hot stamped and die quenched sheets of Boron steel was carried out using 3kW Nd/YAG laser. The appropriate Lap joint laser welding conditions were founded separately for four lap joint combinations. The lower sheest is a hot stamped sheet in common and the upper sheet is selected among the hot stamped steel and high strength steels such as SPCC, 370MPa, and 590MPa grade high strength steels. Cross bead sections and local hardening and softening were observed as well as tensile-shear test results.

• International Journal of Advanced Culture Technology
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• v.3 no.1
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• pp.145-152
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• 2015

The article aims to apply a friction stir spot welding for producing the lap joint between AA5052 aluminum alloy and C11000 copper alloy. The dimension of the materials was 100 mm in length, 30 mm in width and 1.0 mm in thickness. The copper plate was set overlap the aluminum plate by 30 mm. The welding parameter was the rotating speed of 2500-4000 rpm, the pin inserting rate of 2-8 mm/min and the holding time of 6 sec. The mechanical properties test and the microstructure investigation were performed to evaluate the lap joint quality. The summarized results are as follows. The friction stir spot welding could produce effectively the lap joint between AA5052 and C11000 copper. Increase of the rotating speed and holding time directly affected to decrease the tensile shear strength of the lap joint. The optimized welding parameters in this study that indicated the tensile shear strength of 864 N was the rotating speed of 3500 rpm, the pin inserting rate of 6 mm/min and the holding time of 4sec. The experimental results also showed that the hardness of the weld metal was lower than that of the base materials.

• Journal of Life Science
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• v.21 no.5
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• pp.761-765
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• 2011

Salt stability of enzymes is a crucial practical factor in the food industry. Previously, leucine aminopeptidase (LAP) was purified from Bacillus sp. N2. Here, we present the salt effect of LAP using synthetic substrates. LAP had a hydrolytic activity for L-leucine-${\rho}$-nitroanilide in high concentrations of NaCl (up to 4 M), but not for other neutral salts (LiBr, LiCl, NaBr, KBr, and KCl). It hydrolyzed various synthetic di-peptide substrates with hydrophobic and hydrophilic amino acids at the C-terminal Xaa region, in the presence of 0-4 M NaCl. The result indicated that the hydrolytic action of LAP is not dependent on the hydrophobicity of the amino acid side chain at the scissile bond of the substrate. Remarkably, the hydrolytic activity of LAP was 1-3 folds higher than those of other LAPs and aminopeptidases in 4.5 M NaCl, suggesting that NaCl-tolerant LAP might be used in the food industry as cheese and anchovy sauce.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.1
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• pp.9-13
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• 2013

The aim of the present study was to examine the effect of micellar systems on the absorption of beta-lapachone (b-lap) through different intestinal segments using a single-pass rat intestinal perfusion technique. B-lap was solubilized in mixed micelles composed of phosphatidylcholine and sodium deoxycholate, and in sodium lauryl sulfate (SLS)-based conventional micelles. Both mixed micelles and SLS micelles improved the in situ permeability of b-lap in all intestinal segments tested although the mixed micellar formulation was more effective in increasing the intestinal absorption of b-lap. The permeability of b-lap was greatest in the large intestinal segments. Compared with SLS micelles, the effective permeability coefficient values measured with mixed micelles were 5- to 23-fold higher depending on the intestinal segment. Our data suggest that b-lap should be delivered to the large intestine using a mixed micellar system for improved absorption.

• Structural Engineering and Mechanics
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• v.37 no.1
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• pp.111-129
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• 2011

This paper presents a nonlinear analysis of reinforced concrete column with lap splice confined by FRP wrapping in the critical hinging zone. The steel stress-slip model derived from the tri-uniform bond stress model presented in the companion paper is included in the nonlinear frame analysis to simulate the response of reinforced concrete columns subjected to cyclic displacement reversals. The nonlinear modeling is based on a fiber discretization of an RC column section. Each fiber is modeled as either nonlinear concrete or steel spring, whose load-deformation characteristics are calculated from the section of fiber and material properties. The steel spring that models the reinforcing bars consists of three sub-springs, i.e., steel bar sub-spring, lap splice spring, and anchorage bond-slip spring connected in series from top to bottom. By combining the steel stress versus slip of the lap splice, the stress-deformation of steel bar and the steel stress-slip of bars anchored into the footing, the nonlinear steel spring model is derived. The analytical responses are found to be close to experimental ones. The analysis without lap splice springs included may result in an erroneous overestimation in the strength and ductility of columns.

• Oncology Letters
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• v.42 no.4
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• pp.1621-1630
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• 2019

One million females are diagnosed worldwide every year with breast cancer, and the mortality rate of these patients remains high. Several treatments, including surgery, are available for breast cancer. β-Lapachone (β-Lap), a natural quinone compound, has been developed for cancer treatment due to its strong cytotoxic effect through its action on NAD(P)H:quinone oxidoreductase 1 (NQO1)-dependent activity. However, the mechanism in regards to how β-Lap induces cytotoxicity in breast cancer cells is still elusive. In the present study, we showed that β-Lap induced apoptotic cell death via activation of protein kinase A (PKA) in NQO1-overexpressing MDA-MB-231 human breast cancer cells. This PKA-dependent cell death was observed solely in NQO1-overexpressing 231 cells via the high production of reactive oxygen species (ROS). Cell survival of antioxidant [N-acetylcysteine (NAC)]-treated NQO1-overexpressing 231 cells was significantly recovered, and NQO1-negative 231 cells did not respond to β-Lap. Antiapoptotic proteins such as Bcl2 and Bcl-xL were decreased, while proapoptotic proteins, including cytochrome c, activation of caspase-3, and cleavage of PARP were increased after β-Lap treatment of NQO1-overexpressing 231 cells. Furthermore, PKA activators, forskolin or dibutyryl-cAMP, an analog of cAMP, aggravated the β-Lap-induced apoptotic cell death by decreasing antiapoptotic proteins and further activating proapoptotic proteins in NQO1-positive 231 cells. Treatment with a PKA inhibiter, H89, significantly increased cell viability even in NQO1-overexpressing cells treated with β-Lap. These data showed that β-Lap activated PKA via ROS accumulation, subsequently leading to apoptotic cell death in NQO1-positive breast cancer cells.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.21 no.5
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• pp.848-854
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• 2012

SCM420 steel cutting gear to improve the durability is quenched. When quenching, increases surface hardness, a change of the physical properties and machinability or fall. This study, using a solid carbide hobs skiving hobbing gear cutting finishing. And cutting tool solid carbide TiAlN coating hove when TiAlN coating on the surface of multi-con polishing hob conducted aero lap nano polishing for each cutting. Experimental results conducted aero lap nano coating on the surface polishing tool machinability was excellent. And aero lap nano polishing tool results were reduced 2.5 times the tool wear compared to TiAlN coated tools. Excellent results were 1.42 times longer tool life.

• International Journal of Advanced Culture Technology
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• v.3 no.1
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• pp.153-160
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• 2015

This article is aimed to study the effects of resistance spot welding (RSW) on the lap joint properties between AA1100 aluminum alloy and SGACD zinc coated steel and its properties. The summarized experimental results are as follows. The summarized experimental results are as follows. The optimum welding parameters that produced maximum tensile shear strength of 2200 N was a welding current of 95 kA, a holding time of 10 cycles, and a welding pressure of 0.10 MPa. Increasing of welding current, increased the tensile shear strength of the joint and also increased the amount of aluminum dispersion at the joint interface. The lap joint of steel over the aluminum (Type I) showed the higher joint tensile shear strength than a lap joint of aluminum over the steel (Type II). The indentation depth and the ratio of the indentation depth to the plate thickness decreased when the welding current was increased in the type I lap joint and also decreased when the welding current was decreased in the type II lap joint. The interface structure showed the formation of the brittle $FeAl_3$ intermetallic compound that deteriorated the joint strength.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.5
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• pp.570-576
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• 2018

In reinforced concrete structures, the joints of ordinary rebars are usually lap joints, which are bound by binding wires with rebars, and mechanical joints by couplers. In domestic design standards (concrete design code), welded lap joints are restricted for ordinary rebars, but overseas standards allow welded lap joints of ordinary rebars through pre-heating. This study investigated the domestic and international standards/criteria and evaluated the fracture strength by performing the tensile test on the lap welded joint of SD400 grade rebars, which is used the most in the construction sites. The weld length of the specimen for weld lap joints is based on the minimum weld length (8d) given in the KS standard (KS B ISO 17660-1). According to AWS D1.4, the preheating temperature was set to $150^{\circ}C$ for D19 and below, and $260^{\circ}C$ for D22 and above. In the test results, the tensile strength of rebars with welded lap joints exceeded the required strength (125% of the yield strength) according to the concrete design code. To analyze the effect of preheating, the tensile strength of the welded rebars after preheating was not significantly different from that of the welded rebars without preheating. The carbon equivalent content (Ceq) of the rebars used in the test was 0.45% or less. Under AWS D1.4, no preheating is required if the carbon equivalent is less than 0.45%. All specimens with a welded lap length of 8d failed by a bar fracture. The effect of preheating was confirmed to be insignificant due to the low carbon equivalent of the rebar.

• Structural Monitoring and Maintenance
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• v.1 no.3
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• pp.273-292
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• 2014

The effectiveness of a retrofitting method for concrete columns with particular weaknesses is experimentally evaluated and presented in this paper. Structural deficiencies namely the inadequacy of transverse reinforcement and short length of lap splices are very common in columns found in structures built prior to the 1960s and 1970s. Recent earthquakes worldwide have caused severe damages and collapses of these structures. Nevertheless, the importance of improving the load transfer capacity between the deficiently lap-spliced bars is usually underestimated during the strengthening procedures applied in old buildings, though critical for the safety of the residents' lives. Thus, the seismic performance of the enhanced columns is frequently overestimated. The retrofitting approach presented herein involves reinforced concrete jacketing of the column sub-assemblages and welding of the lap-spliced bars to prevent the splice failure and conform to the provisions of modern design Codes. The cyclic lateral loading response of poorly confined original column specimens with insufficient lap splices and the seismic behavior of the retrofitted columns are compared. Test results clearly demonstrate that the retrofitting procedure followed is an effective way of significantly improving the seismic performance of substandard columns found in old buildings.