• 대한임상검사과학회지
• /
• 제45권2호
• /
• pp.43-47
• /
• 2013

This study was designed to evaluate the prevalence of ESBL genes and monitor antimicrobial resistance pattern in Escherichia coli, isolated from a hospital and a community. We tested 200 E. coli strains isolated in the hospitals and community in Chungcheong area from January to March 2012. Antimicrobial susceptibilities were tested by using the disk diffusion method. A search for ESBL genes was conducted by PCR amplification, and the genotypes were determined by direct nucleotide sequence analysis of the amplified products. An Epidemiologic study was performed by repetitive extragenic palindromic sequence-based PCR (REP-PCR). The percentage of ESBL-producing isolates was 17% for hospital associated E. coli and 11% for community associated E. coli. The ESBL gene sequencing results showed that the most common ESBL in E. coli was CTX-M-14 (19/28), followed by CTX-M-15 (9/28). The REP-PCR study also showed the genetic diversity, but there was no difference between the hospital and community associated E. coli. In this study, the most common types of class A ESBLs identified were CTX-M in the hospital and the community in Chungcheong area. ESBL-producing E. coli isolates showed diverse clonality.

• Molecules and Cells
• /
• 제42권1호
• /
• pp.45-55
• /
• 2019

The liver is involved in a wide range of activities in vertebrates and some other animals, including metabolism, protein synthesis, detoxification, and the immune system. Until now, various methods have been devised to study liver diseases; however, each method has its own limitations. In situ liver perfusion machinery, originally developed in rats, has been successfully adapted to mice, enabling the study of liver diseases. Here we describe the protocol, which is a simple but widely applicable method for investigating the liver diseases. The liver is perfused in situ by cannulation of the portal vein and suprahepatic inferior vena cava (IVC), with antegrade closed circuit circulation completed by clamping the infrahepatic IVC. In situ liver perfusion can be utilized to evaluate immune cell migration and function, hemodynamics and related cellular reactions in each type of hepatic cells, and the metabolism of toxic or other compounds by changing the composition of the circulating media. In situ liver perfusion method maintains liver function and cell viability for up to 2 h. This study also describes an optional protocol using density-gradient centrifugation for the separation of different types of hepatic cells, allowing the determination of changes in each cell type. In summary, this method of in situ liver perfusion will be useful for studying liver diseases as a complement to other established methods.

• Journal of Microbiology and Biotechnology
• /
• 제30권7호
• /
• pp.996-1004
• /
• 2020

Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorption-desorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.

• 교육시설
• /
• 제8권3호
• /
• pp.25-35
• /
• 2001

This study investigated technology education, laboratory management and facilities in middle schools with the goal of finding problems. Ninety-six randomly selected schools and questionnaire was done to the teachers of 84 schools which have laboratory. Five of these schools received on-site inspections. The results of study are as follows : 1) 12.5% of schools have no laboratory. The average laboratory has less than 50% of the necessary equipment for total operations. laboratories are used an average of once or twice per two time frame. 2) 80%of the laboratories are located at the end of a row of regular class rooms. The most common laboratory style is classroom plus hallway($90m^2$) style. 77% of laboratories have a single entrance. 3) 69% of teachers reported the worktable arrangement to be inconvenience. 68% of the schools have 9 worktables per laboratory. 38% say the worktables are not wide enough and 36% said they were appropriate. Table dimensions range from $95{\sim}110cm$ in width and from $210{\sim}225cm$ in length. 65% of the teachers say table height is low with a range from $76{\sim}82cm$. 86% of the teachers say that the height of chair is low and the range of its measurement is from $37{\sim}47cm$. 84% of the teachers points out that laboratory is poorly lighted.

• 한국가스학회지
• /
• 제16권5호
• /
• pp.21-27
• /
• 2012

대학 실험실이나 연구시설에서 실험실 사고에 의해 인적 및 물적 손실이 꾸준히 발생되고 있어서 실험실 사고예방의 필요성이 제기되고 있다. 실험실 사고예방을 위해 무엇보다 중요한 것은 사고의 체계적인 분석이다. 국내에서 산업재해에 관한 분석 연구는 수행되어 왔으나, 실험실 사고 분석에 관한 연구는 수행되지 않았다. 본 연구에서는 실험실 사고예방 대책 수립을 위해 설문지를 개발하였고, 설문지는 대학과 연구기관에 전자우편이나 방문조사로 사고사례를 수집하였다. 사고사례 데이터는 계절별, 사고 분류형태, 발생형태, 기인물 및 상해종류 별로 분석 고찰하였다. 이 연구결과는 실험실 종사자의 안전 확보와 사고예방을 위한 기초자료로 활용하고자 하였다.

• Parasites, Hosts and Diseases
• /
• 제58권2호
• /
• pp.161-171
• /
• 2020

The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.

• Animal Bioscience
• /
• 제36권6호
• /
• pp.829-839
• /
• 2023

Objective: The aim of this study was to clone the mRNA sequence of the Acyl-CoA dehydrogenase long chain (ACADL) gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis. Methods: We obtained the ACADL gene of goats by cloning and used quantitative real-time polymerase chain reaction (qPCR) to detect the ACADL expression patterns of different goat tissues and subcutaneous fat cells at different lipid induction stages. In addition, we transfect intramuscular and subcutaneous adipocytes separately by constructing overexpressed ACADL vectors and synthesizing Si-ACADL; finally, we observed the changes in oil red stained cell levels under the microscope, and qPCR detected changes in mRNA levels. Results: The results showed goat ACADL gene expressed in sebum fat. During adipocyte differentiation, ACADL gradually increased from 0 to 24 h of culture, and decreased. Overexpression of ACADL promoted differentiation of subcutaneous adipocytes in goat and inhibited their differentiation after interference. Conclusion: So, we infer ACADL may have an important role in positive regulating the differentiation process in goat subcutaneous adipocytes. This study will provide basic data for further study of the role of ACADL in goat subcutaneous adipocyte differentiation and lays the foundation for final elucidating of its molecular mechanisms in regulating subcutaneous fat deposition in goats.

• 한국과학교육학회지
• /
• 제26권3호
• /
• pp.342-355
• /
• 2006

이 연구는 과학교육에서 중요한 위치를 차지하고 있는 실험수업의 유형을 분류할 수 있는 틀을 개발하는데 목적이 있다. 분류틀을 개발하기 위해 실험의 유형에 관한 선행연구들을 분석하고, 실험수업의 다양한 특성들을 분명하게 나타내는 실험수업 분류틀(CSLI)을 생성하였다. 실험수업 분류틀(CSLI)은 실험수업에 따르는 절차와 접근방식의 두 개의 분류자로 구성하였다. 실험수업에 따르는 절차는 절차 제시의 주체에 따라 절차가 외부에서 공급되는 경우와 학생들에 의해 설계되는 학생생성으로 구분하였으며, 실험수업의 접근 방식은 개념 제시의 순서에 따라 연역적 접근과 귀납적 접근으로 이분법적으로 구분하였다. 두 개의 분류자에 의해 실험수업을 네 가지 유형 - 확인실험, 발견실험, 탐색실험, 연구실험 -으로 분류하고, 각 유형의 실험수업들의 특성과 장단점을 기술하였다. 개발한 실험수업분류틀에 대해서 과학교육전문가 6인의 타당도 검증을 거쳐 분류틀을 완성하였다. 개발된 실험수업 유형 분류틀이 현장검증의 부족으로 인해 실험수업의 다양한 특성을 제한적으로 나타낼지라도 보다 나은 연구를 위한 모델로서 공헌할 수 있을 것이다.

• 한국안전학회지
• /
• 제31권4호
• /
• pp.126-135
• /
• 2016

The purpose of this study is to develop risk assessment techniques and institutional analysis of domestic and international, the management techniques that can efficiently manage the harmful factor of the laboratory and to present the institutional measures that can be efficiently implemented. Due to a variety of adverse factors of laboratory, accidents of laboratory of various forms have occurred, but there is no risk assessment system in order to manage this effectively. So, we investigated this domestic existing risk assessment methods and the outside of the risk assessment system, and also analyzed accidents of domestic laboratory that occurred in 2014. In addition, we targeted the 24 laboratories in 21 universities to investigate the management of harmful factors of the laboratory and performed applying test for 12 domestic laboratories. Existing risk evaluation system, such as PMS, SMS, off-site impact assessment, since the industrial site is the subject, is a difficult problem to be directly applied to the laboratory of the research institute. So, we implemented management status and harmful factors survey and classified the research and development activities based on this data. Finally we developed "pre-hazards risk analysis method" to create each of the safety management measures. In addition, research activities personnel conducted voluntarily risk assessment, which is shared by institutions and government. It is presented the institutional system for safety management of laboratory. Its result, pre-hazards risk analysis method and institutionalization scheme will be able to achieve laboratory accident prevention system.

• 대한치과기공학회지
• /
• 제35권4호
• /
• pp.377-385
• /
• 2013

Purpose: This research conducted by using Department of Dental Laboratory Technology students investigates interpersonal and emotional intelligence impact on psychological happiness. Methods: Study was conducted in the Daegu-Gyeongbuk area Department of Dental Laboratory Technology students, based on 132 people who were enrolled college students. Data analysis, frequency analysis, factor analysis, reliability test, correlation analysis, multiple regression analysis was carried out and SPSS/WIN 18.0 was used. Results: Interpersonal factors utilize emotions, emotional self-control and emotional awareness. Others turned positive (+) was found to affect the psychological, emotional factors utilized in euphoria, emotional adjustment and were found to have positive effects. Conclusion: Looking at the above results gathered from dental engineering students, improvement in interpersonal relationships, and psychological welfare have improve more effectively through utilizing and controlling emotions, including the ability to set their own goals for the future. Motivational training programs for strengthening study habits is more important than what can be based in dental college engineering students. In conjunction with an active lifestyle, future professional dental health services for patients and clinicians care about the improvement of his own psychological happiness was inspire by the care received from home and school and the resulting map is considered to require constant attention.