• Journal of the korean society of oceanography
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• v.39 no.3
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• pp.163-171
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• 2004

Sequences of the large subunit ribosomal (LSD) rDNA D1-D2 region of Alexandrium catenella(=A. sp. cf. catenella) and A. tamarense isolates, which were collected along the Korea coasts, were analyzed to understand their phylogenetic relationships and geographical distributions. All A. catenella and A. tamarense isolates belonged to the A. tamarense/catenella/fundyense complex and were grouped with the North American and temperate Asian ribotypes, respectively, regardless of the presence or absence of a ventral pore in the first apical plate. A consistent and peculiar characteristic that differentiated the Alexandrium isolates was amplification of a second PCR product with a lower molecular weight in addition to the predicted one; ten A. catenella isolates belonging to the temperate Asian ribotype yielded this additional PCR product. Sequence alignment revealed that the shorter PCR product resulted from an unusual large deletion of 87 bp in the LSD rDNA D1 domain. The North American and temperate Asian ribotypes were prevalent along the Korean coasts without geographical separation. Given the high genetic homogeneity among widely distributed Alexandrium populations, each ribotype appeared to be pandemic rather than to constitute a distinct regional population.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.71.2-71
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• 2003

Ralstonia solancearum infects many solanaceous plants, however race 3 infects only potato and tomato weakly. To identify genes responsible for race specificity of R. solanacearum, we mobilized genomic library of LSD2029 (race 3) into LSD341 (race 1) and inoculated 1,000 transconjugants into hot pepper. One transconjugant that did not induce wilt symptom in hot pepper was isolated. We found that a cosmid clone, pRSl, conferred avirulence to LSD341. By deletion and mutational analyses of pRSl, we found the 0.9-kb PstI/Hindlll fragment carries avirulence functions. We sequenced the fragment and identified one possible open reading frame, a rsal gene, possibly encoding 110 amino acids. The rsal was preceded with a plant-inducible promoter (PIP) box, indicating that the gene might be regulated by HrpB. Interestingly, the promoter region of the rsal homolog in the strain GM11000 (race 1) did not have the PIP box. Rsal did not show any significant homologies with proteins in the database, indicating th e protein is different from the previously reported avirulence proteins. When we mutated the rsal gene by marker-exchange in LSD2029, the mutant was less virulent in potato.

• Journal of Korean Society of Steel Construction
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• v.17 no.2 s.75
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• pp.207-216
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• 2005

The maximum strength of the stainless steel square and the circular hollow section columns, which are cold-formed and TIG welded, is experimented on and analyzed. The paper presents centrally compressed experiments, including stub column tests and coupon tests, on stainless steel pipe columns. A total of 24 stainless steel pipe column experiments are conducted, using the slenderness ratios ($L_k/r$ = 20, 30, 40, 50, 60, 70) as parameters. The experimental results were compared with the design standard curves, AIK-LSD and AISC-LRFD, AIJ-LSD, SIJ-ASD curves, and multiple column curves.

• Korean Journal of Biological Psychiatry
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• v.3 no.2
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• pp.245-250
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• 1996

This research was performed to find out the changes of serotonin receptors in the platelet of alcoholics. Using $_{125}I-LSD$ as a radioligand. We obtained the following results. 1) In the comparison of patients vs controls, $B_{max}$ of the former was significantly higher than that of the latter(P=0.0017). But there was no significant difference in the Kd between the two groups. 2) In the comparison of type 1 vs type 2, alcoholics by cloninger's classification $B_{max}$ of the latter was significantly higher than that of the farmer(P=0.0396). But there was no significant difference in the Kd between the two groups. 3) In the comparison of alcohol abuse vs alcohol dependence, there were no significant differences in the value of $B_{max}$ and Kd between the two groups. These results support the hypothesis of serotonin-deficit in alcoholism. Furthermore, it can be inferred that the increase of platelet serotonin receptors in alcoholics might be used as a trait marker.

• Journal of the Institute of Convergence Signal Processing
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• v.13 no.4
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• pp.173-179
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• 2012

In this paper, we presents a new method for vehicle number plate detection. Our method is basically the method extracting a rectangles from a car image because the shape of a vehicle number plate is a rectangle. For detecting the vehicle number plate, firstly, the contrast of the input image is enhanced. Then, the lines in the image are obtained by using LSD(line segment detector), and rectangles in the image are detected from the line data. These rectangles are the candidates of the car plate, from which the car plate is selected. In this procedure, the method of detecting rectangles is our proposed method, which consists of three stages: (1) extracting corners from the line segments by LSD; (2) extracting diagonal lines from the corner data; and (3) detecting rectangles from diagonal line information. And finally the vehicle number plate is selected from these rectangles by using the feature of the vehicle number plate and the inside information of rectangles. In the experiments with the 100 images captured by our digital camera, we have achieved a detection rate of 94%.

• Korean Journal of Veterinary Service
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• v.45 no.2
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• pp.133-137
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• 2022

Lumpy skin disease (LSD) is a severe cross-border infectious disease that causes fever, skin and visceral nodules in cattle. LSD is caused by the lumpy skin disease virus (LSDV), a dsDNA virus that belongs to the genus Capripoxvirus. Although LSD has been found only in Southern Africa traditionally, in the last decade it is spreading very quickly through the Middle East and into Eastern Europe and China. It usually affects cattle and water buffalos being transmitted by blood-feeding insects. As it causes a huge economic impact, LSD is a notifiable disease by World Organisation for Animal Health, and managed as the legal infectious disease class I in Korea. Therefore, the purpose of this study was to confirm the existence of LSDV antigens or antibodies in Korean livestock. We collected 1,200 blood samples from cattle (Korean native and dairy cattle) and Korean water deer in 4 major provinces of the country, then tested the existence of LSDV antigen and antibody. None (0.0%) of the 1,200 blood samples were positive for both antigen and antibody of LSDV. To the best of our knowledge, this is the first study that examines the prevalence of LSDV in Korea. Our study aims to report the LSDV occurrence situation obtained by surveillance in Korea and provide information that may help prevention of LSD epidemics.

• BMB Reports
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• v.56 no.12
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• pp.657-662
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• 2023

Neurodegenerative diseases are characterized by distinct protein aggregates, such as those of α-synuclein and tau. Lysosomal defect is a key contributor to the accumulation and propagation of aberrant protein aggregates in these diseases. The discoveries of common proteinopathies in multiple forms of lysosomal storage diseases (LSDs) and the identification of some LSD genes as susceptible genes for those proteinopathies suggest causative links between LSDs and the proteinopathies. The present study hypothesized that defects in lysosomal genes will differentially affect the propagation of α-synuclein and tau proteins, thereby determining the progression of a specific proteinopathy. We established an imaging-based high-contents screening (HCS) system in Caenorhabditis elegans (C. elegans) model, by which the propagation of α-synuclein or tau is measured by fluorescence intensity. Using this system, we performed RNA interference (RNAi) screening to induce a wide range of lysosomal malfunction through knock down of 79 LSD genes, and to obtain the candidate genes with significant change in protein propagation. While some LSD genes commonly affected both α-synuclein and tau propagation, our study identified the distinct sets of LSD genes that differentially regulate the propagation of either α-synuclein or tau. The specificity and efficacy of these LSD genes were retained in the disease-related phenotypes, such as pharyngeal pumping behavior and life span. This study suggests that distinct lysosomal genes differentially regulate the propagation of α-synuclein and tau, and offer a steppingstone to understanding disease specificity.

• Asia pacific journal of information systems
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• v.6 no.1
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• pp.241-267
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• 1996

The problems like exceeding estimated cost, late due-date, expensive maintenance, insuffiency of experts and low productivity are becoming emphasized related with the software development. In order to overcome these problems and to develop the highly qualified software within the limited resources, a project management tool is used. The purpose of this study is to develop a conceptual model of the project management system which can plan, analyze and control the software development projects effectively. The model is constructed with plan and track management system, cost management system and operation management system for the efficient project management. It is named by LSD-PMS : Large-scale Software Development Project Management System. We reviewed 5 cases of project management. LSD-PMS is evaluated and reviewed by the project managers in the field. It is proved that LSD-PMS is a tool which can help project managers develop software successfully given budget and time schedule. In the future, this system should be further developed as an integrated model with system implementation tools such as CASE products.

• Journal of the Korea Safety Management & Science
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• v.12 no.2
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• pp.217-223
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• 2010

The research proposes the complementary methodology using integrated hypothesis testing and confidence interval models that can be identified the statistical difference and practical equivalence. The models developed in this study can be used in the quality improvement processes such as QC story 15 steps. For the expressions of CI4LSD(Confidence Interval for Least Significant Difference) and CI4TOST(Confidence Interval for Two One-Sided Tests) are simple, quality practioners can efficiently handle them. CI4TOST models as a complement can be applied when CI4LSD models are influenced by sample size and precision.

• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.884-886
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• 2001

The reactivity and stability of purified Lignostilbene- ${\alpha}$,${\beta$}-dioxygenase (LSD)-I were examined. Its optimum temperature was $50^{\circ}C$ at pH 8.5, but it was stable only up to $30^{\circ}C$. The activity of LSD-I increased 12-fold by $30\%$, with increased $V_{max}$ and lowered $K_m.$ LSD-I was stable in 10% methanol.