• 한국응용과학기술학회지
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• 제30권2호
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• pp.272-279
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• 2013

본 연구에서는 중국산 발효 보이차 잎을 고농축으로 추출하였다. 또한 이 추출물에 대한 항산화 활성 및 항노화 효과를 평가하여, 화장품 산업에 응용하고자 하였다. 발효보이차 추출물의 수득율은 17.9%이었으며, 총 폴리페놀량은 37.5%, 탄닌 7.5%, 다당체류 25%, 기타 22%, 수분 8%가 함유되어 있었다. 발효보이차 추출물의 항산화 (in-vitro)활성은 30mg/mL에서는 $33.7{\pm}1.8%$, 50mg/mL에서는 $39.4{\pm}2.2%$의 소거효과를 보였다. 비교군인 초산토코페롤과 녹차추출물 보다 높은 항산화 효과를 보였다. 발효보이차 추출물의 콜라겐 합성율(in-vitro)은 1 mg/mL에서는 $102.9{\pm}9.9%$, 5 mg/mL에서는 $111.5{\pm}9.9%$, 10 mg/mL에서는 $122.7{\pm}12.2%$, 30 mg/mL에서는 $131.5{\pm}13.7%$의 높은 합성능을 보였다 (*p-value£0.05, n=3). 8시간 후의 피부보습효과(in-vivo)는 비교군보다 38.5%의 높은 효과를 보였다. 이 보습력은 도포전보다 32.7% 상승하였다. 발효보이차 추출물은 화장품산업에서 보습효과를 가진 원료로 응용이 가능할 것으로 기대한다.

• Journal of Ginseng Research
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• 제19권1호
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• pp.51-55
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• 1995

The DNA fragment containing ginseng ribulose-1,5-bisphosphate carboxytase/oxygenase large subunit(rbcL) gene was cloned from the ginseng chloroplast EcoRl library by colony lift hybridization with tobacco rbcL gene probe. From the screened clone, the DNA fragment containing ginseng rbcL gene was digested with several restriction enzyme and analyzed by Southern blot hybridization for the construction of restriction map. The ginseng rbcL gene fragment was subcloned in pBluescript II SK + vector and sequence analysis was performed. The nucleotide sequence of ginseng rbcL gene was compared with those of petunia, tobacco, alfalfa, rice and barley, which showed a homology of 93.1%, 95.2%, 90.5%, 85.5% and 84.3%, respectively.

• Applied Biological Chemistry
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• 제3권
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• pp.25-48
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• 1962

tragacanth gum의 화학구조(化學構造)를 구명(究明)하기 위(爲)하여 미국(美國) 약전(藥典)의 tragacanth gum 분말(粉末)을 가지고 다음의 실험(實驗)을 통(通)하여 이의 성분(成分)의 하나인 polysaccharide C를 분리(分離)하여 이의 화학구조(化學構造)를 밝혔다. (1) tragacanth gum을 85% 주정(酒精)으로 처리(處理)해서 중성다당류(中性多糖類)로 polysaccharide C를 분리(分離)하였으며 구성당(構成糖)으로 L-rhamnose, D-xylose, L-arabinose 및 D-galactose를 paper chromatography와 Cellulose column chromatography로 분리(分離), 동정(同定)하였다. 이의 molar ratio는 2:1:17:9이며 비선광도(比旋光度)는 $[{\alpha}]^{30}_D-72.2이다. (2) 구성당(構成糖)의 결합위치(結合位置)를 구명(究明)하기 위(爲)해 Hawarth 법(法)과 Purdietldir(試藥)을 가지고 methyl화(化)시켜 methyl화(化) polysaccharide C를 얻었으며 비선광도(比旋光度) $[{\alpha}]^{22}_D-102를 보였다. 이것을 가수분해(加水分解)시켜 paper chromatography와 column chromatography를 통(通)해 methyl화단당(化單糖)으로 1,3,5-tri-O-methyl-L-arabofuranose, 3,4-di-O-methyl-L-rhamnose, 2,3-di-O-methyl-D-xylose, 2,3,4-tri-O-methyl-D-galactopyranose, 2,4-di-O-methyl-L-arabopyronose, 2,4-di-O-methyl-D-galactose, 2-O-methyl-L-arabinose 및 L-arabinose를 분리(分離), 동정(同定)하였다. (3) 산(酸)의 각종농도(各種濃度)에 따른 부분적(部分的) 가수분해(加水分解)를 시켜 polysaccharide C의 end group, 측지(側枝) 또는 주쇄(主鎖)를 이루는 구성당(構成糖)을 밝히기 위(爲)하여 0.05 N-HCl로 제1차(第一次) 가수분해(加水分解). 0.1N-HCl로 제2차(第二次) 가수분해(加水分解), 0.3N-HCl로 제3차(第三次) 가수분해(加水分解)를 하여 가수분해물(加水分解物)과 비가수분해물(非加水分解物)에서 각각(各各) 다음과 같은 구성단당(構成單糖)을 검출(檢出)하고 이들의 molar ratio를 측정(測定)하였다. 제1차(第一次) 가수분해물(加水分解物)(A)에서 L-arbinose, 비가수분해물(非加水分解物)(A')에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose; 제2차(第二次) 가수분해물(加水分解物)(B)에서 L-arabinose와 D-galactose, 비가수분해물(非加水分解物)(B')에서 L-rhamnose, D-xylose, L-arabinose, 및 D-galactose; 제3차(第三次) 가수분해물(加水分解物)(C)에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose, 비가수분해물(非加水分解物)(C')에서 D-xylose와 D-galactose를 검출(檢出)하였다. (4) 구성당(構成糖)의 형태(形態)와 구조(構造)를 밝히기 위(爲)해 polysaccharide C에 대한 periodate산화(酸化) 실험(實驗)을 하여 $C_5H_8O_4$당(當) periodate의 소비(消費)와 formic acid의 생성량(生成量)을 측정(測定)하였는데 periodate의 소비량(消費量)은 1.23 mole, formic acid의 생성량(生成量)은 0.78 mole이다.

• 한국조리학회지
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• 제23권1호
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• pp.28-36
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• 2017

This study made macaroon with the addition of Helianthus tuberosus L. powder so as to increase the application of Helianthus tuberosus L. powder (HTP) to food products and look into the quality characteristics depending on an addition ratio. Helianthus tuberosus L. powder 0%, 0.5%, 1.0%, 1.5%, and 2.0% were added, respectively, to make macaroon. As Helianthus tuberosus L. powder was added more, the moisture content tended to decrease more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, lightness and yellowness tended to reduce more significantly (p<0.001). As Helianthus tuberosus L. powder was added more, the content of total phenol compounds and DPPH radical scavenging activity were significantly increased (p<0.001). In terms of texture analysis, hardness, gumminess, cohesiveness and chewiness were significantly increased, as the Helianthus tuberosus L. powder was increased (p<0.05). In terms of sensory test, regarding taste, 0.5% HTP group had the highest points. And as color, flavor, and texture were improved, 1.5% HTP showed the most desirable result in terms of overall acceptability. Results, if Helianthus tuberosus L. powder is added for cooking macaroon, it seems to increase anti-oxidation function and develop functional macaroon with excellent sensory factors.

• 대한통합의학회지
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• 제1권3호
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• pp.29-35
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• 2013

Purpose : To offer coupled motion caused by sidebending in the extension postures in lumbar spine in the football, baseball players and general students. Method : Coupled motion were analysed by three dimensional motion analyser in the sitting position during lateral bending. Result : Regardless of the direction of sidebending at L1-L3, L5-S2 in the extension posture of the lumbar spine, the direction of coupled motion in the baseball and football players were opposite to the direction of sidebending but the direction of coupled motion was same at L3-L5. while, the direction of sidebending at L1-L3, L3-L5 in the extension posture of the lumbar spine, the direction of coupled motion in the general students were same to the direction of sidebending but, the direction of coupled motion was opposite direction at L5-S2. Conclusion : we found a difference of coupled motion between athletes and non-athletes.

• Plant Resources
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• 제6권3호
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• pp.233-241
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• 2003

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

• 한국식품영양과학회지
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• 제8권1호
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• pp.21-24
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• 1979

쌀단백질(蛋白質)의 아미노 강화효과와 과잉강화에 의한 쌀단백질(蛋白質)의 아미노산(酸) 불균형 현상(現像)을 살펴보기 위하여 시판(市販) 백미(白米)에 $0.25{\sim}1.0%$ 수준으로 L-lysin HCI를 강화하여 동물(動物) 사육실험(飼育實驗)을 행(行)한 결과(結果) 다음과 같은 결과(結果)를 얻었다. 1. 강화력이 0.25%일 때 체중증가량(增加量) 및 PER이 가장 높았다. 2. $0.5%{\sim}1.0%$ 수준에선 첨가하지 않은 대조군보다 PER이 높았으나 0.25% 보다는 낮았다. 3. 강화수준 1.0%까지는 아미노산(酸) 불균형으로 인한 강화 역효과는 나타나지 않았다.

• The Korean Journal of Pain
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• 제30권1호
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• pp.44-50
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• 2017

Background: Low back pain is a frequent condition that results in substantial disability and causes admission of patients to neurosurgery clinics. To evaluate and present the therapeutic outcomes in lumbar disc hernia (LDH) patients treated by means of a conservative approach, consisting of bed rest and medical therapy. Methods: This retrospective cohort was carried out in the neurosurgery departments of hospitals in KahramanmaraŞ city and 23 patients diagnosed with LDH at the levels of L3-L4, L4-L5 or L5-S1 were enrolled. Results: The average age was $38.4{\pm}8.0$ and the chief complaint was low back pain and sciatica radiating to one or both lower extremities. Conservative treatment was administered. Neurological examination findings, durations of treatment and intervals until symptomatic recovery were recorded. $Las{\grave{e}}gue$ tests and neurosensory examination revealed that mild neurological deficits existed in 16 of our patients. Previously, 5 patients had received physiotherapy and 7 patients had been on medical treatment. The number of patients with LDH at the level of L3-L4, L4-L5, and L5-S1 were 1, 13, and 9, respectively. All patients reported that they had benefit from medical treatment and bed rest, and radiologic improvement was observed simultaneously on MRI scans. The average duration until symptomatic recovery and/or regression of LDH symptoms was $13.6{\pm}5.4$ months (range: 5-22). Conclusions: It should be kept in mind that lumbar disc hernias could regress with medical treatment and rest without surgery, and there should be an awareness that these patients could recover radiologically. This condition must be taken into account during decision making for surgical intervention in LDH patients devoid of indications for emergent surgery.

• 한국약용작물학회지
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• 제8권3호
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• pp.250-258
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• 2000

약용식물의 기내 (in vitro) 배양에 의한 유용물질의 생산을 위하여 대황 (Rheum undulatum L.)의 유묘 조직 절편에 Agrobacterium rhizogenes A4을 감염시켜 형질전환을 유도하였다. 형질전환된 부정 근인 모상근은 WPM 배지에서 RCM 배지의 3.4배의 성장률 증가를 나타내었으며, 지표 물질인 tannin의 생산량 또한 동일 배지에서 가장 높았다. 배지내 식물성장조절물질의 처리에 의한 모상근의 성장 및 tannin 생산촉진 효과는 식물성장조절물질을 처리하지 않은 대조구에 비해 2 mg/L IAA 처리구와 0.5 mg/L ABA 처리구에서 각기 1.7배의 성장 (0.72 g dry weight/flask)과 1.4배의 tannin (0.67 mg/g fresh weight)의 생산량 증가를 가져왔다. 배지내 탄소원인 sucrose 는 3%와 5%에서 최대성장 (0.54 g dry weight/flask)과 tannin 생산 (0.46 mg/g fresh weight)을 보였으며, chitosan의 농도는 50 mg/L 처리구에서 tannin의 생합성에 영향을 주었다.

• 한국축산시설환경학회지
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• 제7권3호
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• pp.165-168
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• 2001

육성비육돈의 분뇨배설량 및 분뇨특성을 조사한 결과 시험기간중 사료섭취량은 3.07 kg/일.두, 음수량은 3.84kg/일.두였으며, 평균 분뇨 배설량은 각각 1.80kg/일.두, 2.76kg/일 .두로 총 4.57kg의 분뇨를 배설하는 것으로 조사되었다. 이화학적 특성에서 돈분의 수분 함량은 74.1%, pH 6.5, 질소 0.89%, $P_2O_5$ 0.46%였으며, BOD 및 SS는 각각 74,224m/l, 261,089mg/l였다. 돈뇨의 경우에는 수분 98.2%, pH 7.5, 질소 0.83%, P$_2$O$_{5}$ 0.05% 였으며, BOD 및 SS는 각각 6,054mg/l, 453mg/l였다.