Cloning of Ribulose-1,5-bisphosphate Carboxylase/oxygenase Large Subunit(rbcL) Gene from Korean Ginseng (Panax ginseng C.A. Meyer)

고려인삼(Panax ginseng C.A. Meyer) Ribulose-1,5-bisphosphate Carboxylase/oxygenase Large Subunit(rbct) Gene의 Cloning

The DNA fragment containing ginseng ribulose-1,5-bisphosphate carboxytase/oxygenase large subunit(rbcL) gene was cloned from the ginseng chloroplast EcoRl library by colony lift hybridization with tobacco rbcL gene probe. From the screened clone, the DNA fragment containing ginseng rbcL gene was digested with several restriction enzyme and analyzed by Southern blot hybridization for the construction of restriction map. The ginseng rbcL gene fragment was subcloned in pBluescript II SK + vector and sequence analysis was performed. The nucleotide sequence of ginseng rbcL gene was compared with those of petunia, tobacco, alfalfa, rice and barley, which showed a homology of 93.1%, 95.2%, 90.5%, 85.5% and 84.3%, respectively.