• International Journal of Industrial Entomology and Biomaterials
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• 제37권2호
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• pp.90-94
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• 2018

In the present study, we investigated the effects of temperature and photoperiod on oviposition of Protaetia brevitarsis. The effects of long- and short-day cycles on oviposition and egg hatching of P. brevitarsis were investigated at different temperatures. Three male-female pairs were confined to oviposition chambers maintained at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, and $35^{\circ}C$, with 16L:8D and 8L:16D photoperiod. Oviposition was observed at all temperatures. The total number of eggs laid per female was between 46.8 and 110.8, and the optimal temperature for oviposition and fertility was between $20^{\circ}C$ and $30^{\circ}C$. Furthermore, it was difficult for the eggs to hatch at $35^{\circ}C$. Fewer eggs were laid under short photoperiod than under long photoperiod at all temperatures. Hatching success was 93.5% at $20^{\circ}C$, 90.9% at $25^{\circ}C$, 71.5% at $30^{\circ}C$ and 37.3% at $35^{\circ}C$ under long-day(16L:8D) condition and Temperature had a strong effect on the time to hatching. Neither oviposition nor subsequent egg hatching was influenced by photoperiod and temperature. The information obtained will be useful for mass rearing P. brevitarsis.

• 한국식품영양학회지
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• 제35권4호
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• pp.276-281
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• 2022

Antioxidant activities and α-glucosidase inhibitory activities of Taraxacum officinale solvent fractions were measured. Extraction yields (relative to raw material) of 50% ethanol, hexane, ethyl acetate, butanol, and water were found to be 10.29, 2.61, 5.54, 2.15, and 0.96%, respectively. Polyphenol and flavonoid contents were high in ethyl acetate extract of Taraxacum officinale at 56.88 mg gallic acid/g and 33.27 mg gallic acid/g, respectively. DPPH, hydroxyl radical scavenging activity, and SOD-like activity measurement (IC₅₀%) of Taraxacum officinale 50% ethanol extract, hexane, butanol, ethyl acetate, and water fractions were 22.64, 18.65, 10.29, 20.81, 20.46 mg/mL, 24.68, 10.69, respectively. It was found to be 9.66, 15.81, 13.77 mg/mL, 32.84, 17.09, 12.73, 33.63, and 33.91 mg/mL, and was high in the ethyl acetate layer. Results showed that α-glucosidase inhibitory activities of Taraxacum officinale solvent fraction were 25.75, 15.93, 35.87, 15.96, and 2.88% for 50% ethanol extract, hexane, butanol, ethyl acetate, and water fractions, respectively.

• 수산해양기술연구
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• 제35권3호
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• pp.291-300
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• 1999

In order to investigate the oceanic condition of fishing ground in the southwestern coastal sea of Korea, the oceanographic observation were carried out by the trainingship of Yosu university on May, August and November in 1998. Main features in the observation are as follows; 1) the ranges of temperature, salinity, density and chlorophyll-a were from $14.5^{\circ}C\;to\;18.0^{\circ}C,\; from\;33.5\textperthousand\;to\;34.5\textperthousand$from 24.0 to 25.5 and from $1.0\mu$g/l in spring (May) from $15.0^{\circ}C\;to\;27.5^{\circ}C,\;from\;0.0\textperthousand\;35.0\textperthousand$9.0 to 26.0 and from 0.5$\mu\textrm{g}$/l to 4.0$\mu\textrm{g}$/l in summer(August), and from $ to 21.0^{\circ}\;31.0\textperthousand$, from 22.0 and from $4.0\mug/l\;to\;20.0\mug/l$ in autumn(November), respectively, 2) the temperature in the coastal region was higher than that in the open ocean while salinity, density and chlorophyll-a were lower, and the convection was identified between the surface and the bottom during in spring and autumn, 3) the chlorophyll-a in the this region was varied in each season, being highly distributed offshore Yosu Bay, 4) on evidence of sea water intrusion toward Kumun island was observed.

• BMB Reports
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• 제35권5호
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• pp.452-458
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• 2002

Exposure to ultraviolet light (UV) induces apoptosis in mammalian cells, The caspase group of proteases is required for the appotosis. This pathway is initiated by a release of cytochrome c from the mitochondria into the cytosol. Several Bcl-2 family proteins can regulate the release of cytochrome c by stabilizing the mitochondrial membrane. Here we show that expression of the endogenous bcl-xL was strongly downregulated in NIH3T3 cells within 2 h after UV-C irradiation, and that of bax was upregulated from 8 h after irradiation. Apoptosis was induced in more than 50% of the NIH3T3 cells 48 h after irradiation. Constitutive overexpression of bcl-xL in NIH3T3 cells protected the UV-induced apoptosis by preventing the loss of mitochondrial membrane potential and the activation of caspase 9. There results suggest that downregulation of Bcl-xL is relevant to UV-induced apoptosis of tibroblasts.

• Natural Product Sciences
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• 제16권4호
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• pp.239-244
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• 2010

Protein tyrosine phosphatase 1B (PTP1B), a negative regulator of insulin signaling, has served as a potential drug target for the treatment of type 2 diabetes. The MeOH extracts of twenty-nine medicinal plants, traditionally used in Vietnam as anti-diabetes agents, were investigated for PTP1B inhibitory activity in vitro. The results indicated that, most materials showed moderate to strong inhibitory activity with $IC_{50}$ values ranging from $3.4\;{\mu}g/mL$ to $35.1\;{\mu}g/mL$; meanwhile, eleven extracts (37.9%) could demonstrate PTP1B activity with $IC_{50}$ values less than $15.5\;{\mu}g/mL$; sixteen extracts (55.2%) could demonstrate PTP1B activity with $IC_{50}$ values ranging from $15.5\;{\mu}g/mL$ to $35.1\;{\mu}g/mL$. The study may provide a proof, at least in a part, for the ethno-medical use in diabetes disease of these plants.

• 한국환경보건학회지
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• 제29권3호
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• pp.65-71
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• 2003

The injection concentration of corrosion inhibitor increases under the pH 7, temperature of 2$0^{\circ}C$, and alkalinity of 35 mg/l (as CaCO$_3$), the corrosion rate gradually decreased. When the corrosion inhibitor of 10 mg/l is injected, the corrosion rate for carbon steel pipe, galvanized steel pipe, and copper pipe reduces for 37, 66 and 61 % respectively that it is more efficient on galvanized steel pipe and copper pipe. As a result of examination of corrosion rate at pH 6, 7, and 8 when injecting 10 mg/l of corrosion inhibitor under the conditions of 2$0^{\circ}C$ in water temperature and 35 mg/l (as CaCO$_3$) in alkalinity, the efficiency of the corrosion inhibitor increases as the pH increases. For carbon steel pipe, it does not show much a difference with the change of the pH condition, but galvanized steel pipe and copper pipe clearly show the corrosion rate depending on the change of the pH condition. The efficiency of corrosion inhibitor is low as the concentration of residual chlorine is high, but it does not show a great influence at 0.4 mg/l or less. For each pipe type, in the case of carbon steel pipe, the range of increase of corrosion speed following the residual chloride is higher than the other pipe types. In the meantime, the effect following the residual chlorine in copper pipe is low.

• Food Science and Biotechnology
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• 제14권6호
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• pp.836-840
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• 2005

Extraction characteristics of Bonus species of Brassica oleracea var. capita and functional properties of corresponding extract were monitored by response surface methodology (RSM). Maximum extraction yield of 44.07% was obtained at ratio of solvent to sample of 27.94 mL/g, ethanol concentration of 24.35%, and extraction temperature of $55.21^{\circ}C$. At ratio of solvent to sample, ethanol concentration, and extraction temperature of 21.11 mL/g, 58.53%, and $68.83^{\circ}C$, respectively, maximum electron-donating ability was 48.44%. Maximum inhibitory effect on tyrosinase was 68.94% at ratio of solvent to sample, ethanol concentration, and extraction temperature of 24.08 mL/g, 10.49%, and $78.71^{\circ}C$, respectively. Superoxide dismutase (SOD) showed maximum pseudo-activity of 24.78% at ratio of solvent to sample of 22.66 mL/g, ethanol concentration of 45.69%, and extraction temperature of $93.81^{\circ}C$. Based on superimposition of four-dimensional RSM with respect to extraction yield, electron-donating ability, and pseudo-activity of SOD, optimum ranges of extraction conditions were ratio of solvent to sample of 20-30 mL/g, ethanol concentration of 35-65%, and extraction temperature of $50-80^{\circ}C$.

• Bulletin of the Korean Chemical Society
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• 제35권2호
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• pp.605-609
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• 2014

Magnetic multi-granule nanoclusters (MGNCs) were investigated as an inexpensive means to effectively remove arsenic from aqueous environment, particularly groundwater sources consumed by humans. Various size MGNCs were examined to determine both their capacity and efficiency for arsenic adsorption for different initial arsenic concentrations. The MGNCs showed highly efficient arsenic adsorption characteristics, thereby meeting the allowable safety limit of $10{\mu}g/L$ (ppb), prescribed by the World Health Organization (WHO), and confirming that 0.4 g and 0.6 g of MGNCs were sufficient to remove 0.5 mg/L and 1.0 mg/L of arsenate ($AsO_4{^{3-}}$) from water, respectively. Adsorption isotherm models for the MGNCs were used to estimate the adsorption parameters. They showed similar parameters for both the Langmuir and Sips models, confirming that the adsorption process in this work was active at a region of low arsenic concentration. The actual efficiency of arsenate removal was then tested against 1 L of artificial arsenic-contaminated groundwater with an arsenic concentration of 0.6 mg/L in the presence of competing ions. In this case, only 1.0 g of 100 nm MGNCs was sufficient to reduce the arsenic concentrations to below the WHO permissible safety limit for drinking water, without adjusting the pH or temperature, which is highly advantageous for practical field applications.

• Bulletin of the Korean Chemical Society
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• 제35권2호
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• pp.489-493
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• 2014

A phenylbenzimidazole derivatized sensor (L) that behaves as a ratiometric fluorescent receptor for $Zn^{2+}$ in water has been described. In HEPES buffer at pH 7.4, sensor L displays a weak fluorescence emission band at 367 nm. Upon addition of $Zn^{2+}$, the emission intensity at 367 nm is decreased, concomitantly, a new emission band centered at 426 nm is developed, thus facilitates a ratiometric $Zn^{2+}$ sensing behavior. Sensor L binds $Zn^{2+}$ through a 1:1 binding stoichiometry with high selectivity over other metal cations. Sensor L displays a linear response to $Zn^{2+}$ concentration from 0 to $6.0{\times}10^{-5}M$, sensor L also exhibits high sensitivity to $Zn^{2+}$ with a detection limit of $3.31{\times}10^{-7}M$.

• 한국자원식물학회지
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• 제22권6호
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• pp.506-512
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• 2009

To study the effect of genotype of donor plants on anther culture, anthers of nine hybrid cultivars (Derby, Special, Bossanova, Minipaprika, Fiesta, Boogie, Phenlene, Kufrah, and Clarity) of sweet pepper (Capsicum annuum L.) were cultured in a petridish containing C medium (Sibi, Dumas De Vaulx medium) supplemented with 0.1 mg/L 2,4-D and 0.1 mg/L kinetin, 3% sucrose and 0.32% Phytagel. The cultures were incubated in the dark at $35^{\circ}C$ for seven days, and then cultured at $25^{\circ}C$ with a photoperiod of 16 hr. daylength for 40 days. Frequency of callus formation and plant regeneration was varied among cultivars. Callus formation was ranged from 6% in Phenlene to 69.8% in Kufrah. The highest percentage of regenerated plantlets was obtained in cv. Phenlene (2.67%) followed by Bossanova (2.41%). Result of ploidy analysis; chromosome number observation and flowcytometry analysis, showed that haploid plants could be developed from all of these hybrid cultivars except cv. Fiesta, where highest percentage of haploid plants were obtained in Minipaprika (40%) followed by cv. Bossanova (36.1%). Haploid plants derived from these hybrid cultivars contained single set of chromosome (12 in numbers), higher stomata density (numbers), and smaller sized stomata as compare to diploid plants. The mean length of stomata was 26.9 ${\mu}m$ in haploid plants and 35.7 ${\mu}m$ in diploids.