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Simultaneous Quantitative Analysis of Mixtures of Ixeridium dentatum (Thunb.) Tzvelev, Plantago asiatica L. and Rumex crispus L. Ethanol Extracts (씀바귀, 질경이, 소리쟁이 에탄올 추출 혼합물에 대한 주요 성분 동시 분석)

  • Kim, Ga-Ram;Kim, Eun-Nam;Birasuren, Bayarmaa;Min, Yeonhong;Jeong, Gil-Saeng
    • Korean Journal of Pharmacognosy
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    • v.51 no.3
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    • pp.222-229
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    • 2020
  • Recently, consume for functional cosmetics containing natural products has been greatly increased. In order to develop it as a natural cosmetic material, we selected Ixeridium dentatum (Thunb.) Tzvelev, Plantago asiatica L. and Rumex crispus L. that have antioxidant and anti-inflammatory effects. In this study, simultaneous quantitative analysis of the isolated compounds and natural product complexes (Mix.) were validated using high performance liquid chromatography (HPLC). The isolated six compounds were shown in a large linearity with a correlation coefficient (R2) of 0.99. The limit of detection (LOD) of chlorogenic acid, plantamajoside, acteoside, emodin chrysophanol and physcion were 0.36 ㎍/mL, 0.36 ㎍/mL, 0.37 ㎍/mL, 0.30 ㎍/mL, 0.22 ㎍/mL and 0.12 ㎍/mL, respectively. The limit of quantification (LOQ) of chlorogenic acid, plantamajoside, acteoside, emodin chrysophanol and physcion were 1.10 ㎍/mL, 1.08 ㎍/mL, 1.12 ㎍/mL, 0.99 ㎍/mL, 0.66 ㎍/mL and 0.35 ㎍/mL, respectively. Content analysis showed chlorogenic acid (0.19 ± 0.02%), plantamajoside (0.48 ± 0.01%), acteoside (0.65 ± 0.01%), emodin (1.15 ± 0.11%), chrysophanol (0.73 ± 0.01%) and physcion (0.69 ± 0.09%). Therefore, the results of this study may provide for basic data of standardization research natural cosmetic material development on the I. dentatum, P. asiatica and R. cripus.

The Effect of Pre-chlorination on the Coagulation of Microcystis aeruginosa (전염소처리가 Microcystis aeruginosa 응집에 미치는 영향)

  • Lee, Tae-Gwan;Jin, Jung-Sook
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.3
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    • pp.505-510
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    • 2000
  • In this study the effects of pre-chlorination on the coagulation of water which contain Microcystis aeruginosa. were investigated on the laboratory scale. We prepared the sample of $10^5cell/mL$ Microcystis aeruginosa and then applied 0.2, 1.0, 10 mg-Cl/L chlorine on the sample After reaction period(1 minute and 1 hour), each sample was coagulated. As a result, after 0.4 mg-Al/L coagulant dose, turbidities of all samples were below 2 mg-Kaolin/L. Turbidity was not affected by chlorine dose. As the dose of chlorine was increased, the residual aluminum was decreased, but result of $UV_{254}$ was adverse.

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Biocontrol of Anthracnose of Chili Pepper by Bacillus sp. NAAS-1 (Bacillus sp. NAAS-1을 이용한 고추 탄저병 생물학적 방제)

  • Yoo, Jae Hong;Park, In Cheol;Kim, Wan Gyu
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.277-281
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    • 2012
  • Bacillus sp. NAAS-1 isolated from the field of Chili pepper was tested for biocontrol activity against anthracnose pathogen of Chili pepper caused by Colletotrichum acutatum. The antifungal activity of Bacillus sp. NAAS-1 culture broth was compared with synthetic fungicide containing carbendazim (40%) and kasugamycin (3.45%). Bacillus sp. NAAS-1 showed a similar fungicidal activity against the anthracnose pathogen at the concentration of 50 ${\mu}L/mL$ in comparison to the fungicide containing carbendazim (40%) and kasugamycin (3.45%) using a cup method. Bacillus sp. NAAS-1 also exhibited its potent fungicidal activity against the anthracnose in vivo test at the concentration of 50 ${\mu}L/mL$ when compared to the fungicide containing carbendazim (40%) and kasugamycin (3.45%).

N,N'-Dimethylethylenediamine-N,N'-di-α-butyric Acid Cobalt(III) Complexes Utilizing Oxidation of Sulfur of S-Methyl-L-cysteine

  • Kim, Hyun-Jin;Youm, Kyoung-Tae;Yang, Jung-Sung;Jun, Moo-Jin
    • Bulletin of the Korean Chemical Society
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    • v.23 no.6
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    • pp.851-856
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    • 2002
  • The Reaction of S-methyl-S-cysteine(L-Smc) with racemic $s-cis-[Co(demba)Cl_2]-1$ (Hydmedba = $NN'-dimethylethylenediamine-NN'-di-\alpha-butyric$, acid) yields ${\Delta}$-s-cis-[Co(dmedba)(L-Smc)] 2 with N, O-chelation. Oxidation of sulfur of 2 with $H_2O_2$ in a 1 : 1 mole ratio gives ${\Delta}$-s-cis[Co(dmedba)(L-S(O)mc)] 3 having an uncoordinated sulfenate group. Oxidation of sulfur of L-Sm with $H_2O_2in$ a 1: 1 mole ratio produces S-methyl-L-cysteinesulfenate (L-S(O)me) 5. Direct reaction of 1 with 5 in basic medium gives an N.O-chelated ${\Delta}$s-cis[Co(dmedba)(L-S(O)mc)-N.O], which turmed out be same as obtained by oxidation of 2, while an N, S-chelated ${\Delta}$-s-cis-[Co(dmedba)(S-S(O)mc)-N,O] complex 4 is obtained in acidic medium from the reaction of 1 with 5. This is one of the rare $[$Co^{III}$(N_2O_2-type$ ligand)(amino acid)] type complex preparations, where the reaction conditions determine which mode of N, O and N, S caelation modes is favored.

A geometric criterion for the element of the class $A_{1,aleph_0 $(r)

  • Kim, Hae-Gyu;Yang, Young-Oh
    • Journal of the Korean Mathematical Society
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    • v.32 no.3
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    • pp.635-647
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    • 1995
  • Let $H$ denote a separable, infinite dimensional complex Hilbert space and let $L(H)$ denote the algebra of all bounded linear operators on $H$. A dual algebra is a subalgebra of $L(H)$ that contains the identity operator $1_H$ and is closed in the $weak^*$ operator topology on $L(H)$. For $T \in L(H)$, let $A_T$ denote the smallest subalgebra of $L(H)$ that contains T and $1_H$ and is closed in the $weak^*$ operator topology.

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Regional load deflection rate of multiloop edgewise archwire (Multiloop edgewise arch wire의 부위별 하중변형률)

  • Kim, Byoung-Ho;Yang, Won-Sik
    • The korean journal of orthodontics
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    • v.29 no.6 s.77
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    • pp.673-688
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    • 1999
  • This study was conducted in order to analyze the mechanical characteristics of multiloop edgewise archwire (MEAW). The purposes were 1) to compare load deflection rate (LDR) of MEAW with that of various other arch wires in the individual interbracket span, 2) to compare the wire stiffness in the interbracket span with that in the multi-L-loop region (the span from distal border of the bracket of the lateral incisor to the mesial border of the buccal tube of the second molar), and 3) to verify the experimental results with theoretically derived formula. The single L-loops of five different horizontal lengths and multi-L-loops for the upper and lower arches were made out of .$016\times.022$ permachrome stainless steel wire. Straight segment of plain stainless steel, TMA and NiTi wire of the same dimension were prepared. The LDR was measured using Instron model 4466 with the load cell of 50N capacity at cross head speed of 1.0mm/min, and maximum deflection of 1.0mm. Five specimens were tested under each experimental condition. The wire stiffness number for each interbracket region and multi-L-loop region was calculated from the LDR and the interbracket spans. By dividing the theoretical model of multi-L-loop into 35 linear segments, the energy stored in each segment was obtained. Then the LDR and wire stiffness of single L-loop and multi-L-loop were calculated and compared. The findings were as follows : 1) The average LDR of MEAW in the individual interbracket region was 1/1.53 of that of the NiTi,1/2.47 of TMA and 1/5.16 of the plain stainless steel wire. 2) The wire stiffness of MEAW in the multi-L-loop region was 1.53 times larger than that in the interbracket region, and the LDR was almost twice as large as that of NiTi in that region. 3) According to the theoretically derived equation, the wire stiffness of the single L-loop was lower than that of multi-L-loop. The results of this study suggest that MEAW has the unique mechanical Property which could allow individual tooth movement and transmit elastic force effectively through the entire arch wire.

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Effects of the Molting-Hormone Mimetid Insecticide Tebufenozide: on Chironomus riparius Larvae (탈피교란물질인 Tebufenozide가 Chironomus riparius (Diptera: Chironomidae)에 끼친 영향)

  • 곽인실;이원철
    • Korean Journal of Environmental Biology
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    • v.21 no.3
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    • pp.286-291
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    • 2003
  • We investigated the effects of molting-hormone insecticide tebufenozide on D7 (the day of hatching from egg) larvae of the midge Chironomus riparius in growth developments. D7 instar larvae were exposed test concentrations were chosen control, 10${\mu}g \;L^{-1}$, 30${\mu}g \;L^{-1}$, 60${\mu}g \;L^{-1}$ and 100${\mu}g \;L^{-1}$ of tebufenozide. In general, dead larvae showed 16% on the next day after insecticide treatments (D12), and observed 44% from D12 to D16 in this exposed days. Dead larvae of C. riparius was abruptly increased on D12 and also continuously increased along the days in 10${\mu}g \;L^{-1}$ treatments. The converged day was from D12 to D16 at move 30${\mu}g \;L^{-1}$ treatments in this study. Therefore, dead larvae obviously increased along these concentrations of tebufenozide. In control condition,78% of the test individuals have grown the pupae. But the larvae have developed the pupa stage from 5% to 17% of the test organism in 10${\mu}g \;L^{-1}$ and 30${\mu}g \;L^{-1}$ treatments. And 75% of the test individuals was arrived the adult through the molting process in control condition. While the other condition was rarely observed the adult. Usually, the emerged period of the test individuals was gathered the D26-D29 in control. The dead pupa showed from D19 to D20 in 30${\mu}g \;L^{-1}$ treatments, D32 in control and D33 in 10${\mu}g \;L^{-1}$ treatments. The observed periods of dead pupa were D32-D34 in control and D33-D37 in 10${\mu}g \;L^{-1}$ treatments. Consequently, due to molting hormone disruption, development of midge was postponed relatively low concentration such as 10 treatments of tebufenozide.

Changes of Pulmonary Disability Grades according to the Spirometry Reference Equations (폐기능 예측식에 따른 폐환기능 장해도 변화)

  • Lee, Joung-Oh;Choi, Byung-Soon
    • Tuberculosis and Respiratory Diseases
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    • v.69 no.2
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    • pp.108-114
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    • 2010
  • Background: The aim was to estimate the differences between pulmonary disability grades according to the spirometry reference equations (the Korean equation and the Morris equation). Methods: Spirometry was performed on 16,916 male and 1,353 female special examination for pneumoconiosis, in the period of 2007~2009. Changes in predictive values for forced expiratory volume in one second ($FEV_1$), forced vital capacity (FVC) and $FEV_1$/FVC and in disability grade were evaluated using both equations. Results: Mean FVCs for men and women were 4,218.7 mL and 2,801.5 mL in predictive values after the application of the Korean equation, and 3,763.9 mL and 2,395.6 mL after the Morris equation, respectively. Compared with the Morris equation, the Korean equation showed 10.8% and 14.5% of excesses for men and women (p<0.001). Mean $FEV_1s$ for men and women were 3,102.5 mL and 2,107.1 mL in the Korean equation, and 2,667.8 mL and 1,699.6 mL in the Morris equation, respectively. Compared with the Morris equation, the Korean equation showed 14.0% and 19.3% of excesses for men and women (p<0.001). Men and women who showed the changes of disability grades using the Korean equation in place of the Morris equation were 23.9% (4,052/16,916) and 22.9% (311/1,353) on FVC, and 23.1% (3,913/16,916) and 10.7% (145/1,353) on $FEV_1$. Conclusion: Applying different reference equations for spirometry has resulted in changes for disability grades in special examination for pneumoconiosis.

Characteristics of Nonpoint Source Pollution and Relationship between Land Use and Nutrient Concentrations in the Han River Watershed (강우시 한강유역에서의 비점오염원 유출 특성과 토지이용도와의 관계)

  • Jung, Sungmin;Eum, Jaesung;Jang, Changwon;Choi, Youngsoon;Kim, Bomchul
    • Journal of Korean Society on Water Environment
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    • v.28 no.2
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    • pp.255-268
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    • 2012
  • Nonpoint source pollution has become a concern for water quality in the Han River system, especially during the high runoff events during the monsoon season. The patterns in nonpoint source runoff the relationships with land use, rainfall intensity, and stream nutrients concentrations were surveyed in 19 streams in the Han River watershed. The results show that the magnitude of NPS inputs of nutrients and sediment in the Han River watershed are of a serious concern. In the South Han River watershed, event mean concentrations (EMC) for biochemical oxygen demand (BOD), suspended sediment (SS), dissolved organic carbon (DOC), dissolved total phosphorus (DTP), total nitrogen (TN) Nitrate ($NO_3$-N) and total phosphorus (TP) were $1.94mg{\cdot}L^{-1},\;251mg{\cdot}L^{-1},\;2.75mg{\cdot}L^{-1},\;0.076mg{\cdot}L^{-1},\;2.82mg{\cdot}L^{-1},\;2.40mg{\cdot}L^{-1}$ and $0.232mg{\cdot}L^{-1}$, respectively. In the North Han River watershed, EMCs for BOD, SS, DOC, DTP, TN, $NO_3$-N and TP were $1.34mg{\cdot}L^{-1},\;172mg{\cdot}L^{-1},\;2.63mg{\cdot}L^{-1},\;0.032mg{\cdot}L^{-1},\;1.97mg{\cdot}L^{-1},\;1.55mg{\cdot}L^{-1}$ and $0.148mg{\cdot}L^{-1}$, respectively. The specific export coefficients of nutrient and sediments were much higher than those of other reports. Our study also found that the proportion of agricultural field area was significantly correlated with the EMCs for nutrients. Therefore, efforts to reduce NPS loading must focus on agricultural practices in the watershed. The relationships between land use and nutrient and sediment export found in this study can be used to derive estimates of runoff coefficients for agricultural field and as input data for modeling works and to develop total maximum daily load and best management practices in the Han River watershed.

Spatio-temporal Fluctuation of Phytoplankton Size Fractionation in the Uljin Marine Ranching Area (UMRA), East Sea of Korea (동해 울진 바다목장해역의 크기별 식물플랑크톤 생물량의 시·공간적 분포 특성)

  • Yoon, Yang Ho
    • Korean Journal of Environmental Biology
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    • v.34 no.3
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    • pp.151-160
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    • 2016
  • To understand size fractioned chlorophyll a and material cycles of coastal ecosystem in Uljin marine ranching area (JMRA) of East Sea, 4 times of survey were conducted from April to November 2008. Picoplankton, nanoplankton and netplankton in the surface of UMRA fluctuated with an annual mean of $0.26{\mu}g\;L^{-1}$ between the lowest value of $0.03{\mu}g\;L^{-1}$ and the highest value of $0.87{\mu}g\;L^{-1}$, annual mean $1.32{\mu}g\;L^{-1}$ between $0.11{\mu}g\;L^{-1}$ and $5.60{\mu}g\;L^{-1}$, annual mean $0.45{\mu}g\;L^{-1}$ between no detected (nd) and $4.68{\mu}g\;L^{-1}$, respectively. And the relative ratio of picoplankton, nanoplankton and netplanktons on the phytoplankton biomass was on annual average 12.9%, 65.0% and 22.1%, respectively. The 10 m layer was similar to the surface. The relative ratio of pico- and nano-plankton was higher throughout the year. That is, the material cycle of UMRA consists of a microbial food web rather than traditional food chain at a lower trophic levels. Primary production is deemed to have a higher possibility of being adjusted by top-down dynamics, such as micro-zooplankton grazing pressure rather than nutrients supply.