• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.95-101
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• 2004

This study was undertaken to study the effect of interspecific pollination of L. longiflorum and L. ${\times}$formolongi as the female parent with Oriental, Martagon and Trumpet group as the male parent by cut-style pollination. In the interspecific pollination of L. longiflorum cv. Gelria and Lorina with Oriental group as the male parent, the corresponding fruits obtained immature embryo were 1, 8, and the mean number of embryo per fruit were 11.0, 3.0, respectively. In the interspecific pollination of L. ${\times}$formolongi cv. Raizan, the corresponding fruits obtained immature embryo were 17, and the mean number of embryo per fruit were 4.0. On the other hand, in the interspecific pollination between L. longiflorum and L. ${\times}$formolongi as the female parent and Martagon and Trumpet group as the male parent, the pollination of L. ${\times}$formolongi as the female parent and L. henryi of Trumpet group as the male parent were obtained only 2 fruits, however no embryo.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.353-357
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• 2003

We studies seed set in the interspecific F1 /backcross hybrids of Lilium species. In the interspecfic hybrid of L. longiflorum cv. Gelria with L.${\times}$ fomolongi cv. Raizan,93％ fruit set was obtained by stigmatic pollination in comparison to 53％ from cut-style pollination. Accordingly, the number of seed set resulting from stigmatic and cut-style pollination was 147 and 53, respectively. Pollination o( both stigmatic and cut-style pollination resulted in 47％ fruit set in the hybrid of L. longiflorum cv. Lorina with L.${\times}$ fomolongi cv. Raizan. However, stigmatic pollination formed 413 seeds, whereas only 24 seeds were obtained by cut-style pollination in this cross. The hybrid of L.${\times}$ fomolongi cv. Raizan with L. longiflorum cv. Come set 40％ fruit with a total of 43 seeds by stigmatic pollination. However, no fruit set was observed in cut-style pollination in this hybrid. Backcrossing the F1 hybrid by cut-style pollination of L. longiflorum cv. Lorina ${\times}$ Asiatic hybrid cv. Chicago with the latter parent led to 53％ fruit set, and 109 embryos were obtained. Likewise, backcrossing following cut-style pollination of L. longiflorum cv. Lorina ${\times}$ Asiatic hybrid cv. Corsia with the latter parent formed 67％ fruits and 107 embryos. However, in the remaining interspecific hybrids, cut-style pollination set no fruit.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.347-352
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• 2003

This work was undertaken to study the effect of stigmatic and cut-style pollination on self seed set in Lilium longiflorum and L. ${\times}$ formolongi, and their crosses as the female parent with other cultivars/genotypes. Stigmatic pollination of L. longiflorum cv. Gelria and Lorina resulted in cent per cent fruit set with mean number of seeds/fruit of 189 and 70, respectively. However, cut-style pollination resulted in 87％ and 40％ fruit set in Gelria and Lorina, respectively. The corresponding mean number of seeds/fruit was 53 and 20. In L. ${\times}$ formolongi, stigmatic pollination set 80％ fruits with 736 seeds/fruit. On the other hand, cut-style pollination resulted in 47％ fruit set with 81 seeds/fruit. The intraspecific stigmatic pollination of L. longiflorum cv. Gelria and Lorina with other cultivars formed 60％ fruits with a mean number of 18 seeds/fruit. However, only 20％ fruit set with mean number of seeds/fruit of 7 was recorded when cut-style pollination of L. longiflorum cultivar were pollinated with other cultivars/genotypes. In the intraspecific pollination of L. ${\times}$ formolongi cv. Raizan with Novia, fruit set resulting from stigmatic and cut-style pollination was 75％ and 50％, respectively with the corresponding mean number of seeds/fruit of 579 and 98. It was concluded that self as well as intraspecific seed set in the two species of Lilium gets considerably reduced as a result of cut-style pollination.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.378-383
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• 2001

Embryo culture, ovule culture and ovary slice culture were tested to find optimum method for overcoming post fertilization barrier in interspecific crosses between L. longiflorum 'Gelria' and L. cernuum. Although reciprocal crosses between the species were carried out by cut-style pollination method, fruits developed only in crosses of L. longiflorum${\times}$L. cernuum. On the 40 days after pollination, ovaries were sliced into 2-4mm thickness and cultured on a hormone-free Murashige-Skoog (MS) medium, supplemented with 2%, 4%, 6%, 8% and 10% sucrose. For the L. longiflorum Gelria'${\times}$L. cernuum cross, ovule development was found to be best at 6% sucrose and a lot of hybrid plant lets established directly from the ovary slice culture and subsequent ovule culture. High concentration of sucrose above 8% made ovules abort or vitrificate from 40 days after culture. In contrast, ovules from the L. cernuum${\times}$L. longiflorum 'Gelria' cross swelled well in ovary slice culture, however, they did not germinated in subsequent ovule culture. On the 60 days after pollination, ovules thicker than 0.6mm was interpreted as one containing embryo. The embryo size ranged from 1.2 mm to 1.7 mm, and in vitro development of the excised embryos was found to be best with the MS medium (pH 5.8), supplemented with $0.1-1 mg{\cdot}L^{-1}$ NAA and 6% sucrose. Thick ovules excised 60 days after pollination germinated about 60% as normal seeds in MS medium supplemented with 6% sucrose and free hormone. The ovule culture 60 days after pollination was concluded to be most recommendable to produce interspecific hybrids in large scale crosses between L. longiflorum 'Gelria' and L. cernuum by the reason of easy procedure.

• Korean Journal of Plant Resources
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• v.3 no.2
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• pp.123-128
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• 1990

In every reciprocal crosses of self pollination, interspecific pollination through ordinary stigma pollination of L. longiflorum and $L.{\;}{\times}{\;}elegans$, pollen vigorously germinated in stigma, and pollen tube was growing. But, 5 days after pollination, pollen tube stopping their growth in the same style as was observed in the cross of self pollination and interspecific pollination. Intrastylar pollination of $L.{\;}{\times}{\;}elegans$ and L. longiflorum passed stylar cannal through the basal part of styles. But, pollen tube that was growing to the ovule of the ovary was extremely small. In morphogical observation of ovary 10 days after pollination, it was observed, that every reciprocal crosses of L. longiflorum ${\times}$ L. ${\times}$ elegans was fertilized through intrastylar pollination, so that this technique was used to overcome cross incompatibilities.lities.

• Korean Journal of Plant Resources
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• v.1 no.1
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• pp.80-87
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• 1988

Hybries which was made up by chromosome of L. longiflorum and L. x elegans, using root-tip individual which was obtained through ovary slice culture, and root-tip of these parents, with hoirugen staining, gimsa staining and Q-H staining inaccordance with the location and the existence of secondary construction which waslocating near short arm centromere of No, 1,2,6,9. In metaphase of meiosis ofhybrid which was made up by univalent from 2 individuals to 10 individuals wasobserved, and nuclear plate which was having abnormal type's synthesis amounted to91% of all cells whieh were observed. This result showed the fact that someobstacle arose annormal progress of the divission after that time. 63% of the cellshad micronucleus from 1 individlial to 4 individuals in tetrad phase of meiosisdivision. The peroxidase and $\alpha$ -estelase zymogram phenotypes of parents andhybrids were determined using agarlose IEF gel. Crosses were performed betweenparents bearing dissimilar allelomorphs in orther to discern the genetic control ofthe resolved enzymes. Genetic variation of hybrids were detected at all but 2 plant progenies.

• Journal of Plant Biotechnology
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• v.31 no.3
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• pp.197-201
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• 2004

Shoot clusters were induced from bulb scales of Lilium longiflorum 'Geogia', and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL$.$min$^{-1}$ injection of air was suitable in growth of bulblets.

• FLOWER RESEARCH JOURNAL
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• v.16 no.4
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• pp.304-308
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• 2008

Lilium longiflorum 'Charm' as a $F_1$ hybrid cultivar was released by crossing inbred line '$L_2$-14' and '$L_2$-21' which were obtained from 5 self crosses originated from 'Nellie White', 'Ace' and 'Hinomoto'. The growth and flowering characteristics were evaluated in a greenhouse maintained at a minimum of $13^{\circ}C$ at night during winter in 2006 and 2007. The flower of 'Charm' is white color and horizontal-facing. The flower number of a plant and its diameter is 7.4 and 16.5 cm with 24.5 ornamental(flowering) days. The plant height is 60.3 cm with 70.3 number of leaves. The stem diameter and internode length is 1.2 cm and 1.1 cm, respectively, meaning the plant is compact and sturdy. And the number of seed per a capsule is 251.1. The results of these evaluation, therefore, suggest that seedling Lilium longiflorum 'Charm' can be used as a pot plant due to its short stems, many number of flowers per plant, long ornamental period, strong growth habit with many leaves and thick stem diameter.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.308-315
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• 2009

The activities of flower color biosynthesis-controlling enzymes, flavonoid 3'hydroxy lase (F3'H) and dihydroflavonol 4-reductase (DFR), were analyzed in Llium longijlorum and 11 lily cultivars using biochemical and enzymological methods. Dihydroquercetin (DHQ) product was synthesized by F3'H using dihydrokaempferol (DHK) as a substrate in Lilium longiflorum. F3'H activity was also detected in the eight cultivars tested. The substrate-specific activity of F3'H was observed because eridictiol (ERI), which uses naringenin (NAR) as a substrate, was not detected in the tested cultivars. Leucocyanidin (LCy) product was synthesized by DFR using DHQ as a substrate in Lilium longiflorum. DFR activity was also detected in 'Le Reve', 'Montreux', 'Monte Negro', 'Etude', 'Acapulco', and 'Star Gazer', but not in 'Siberia', 'Royal Race', 'Nove Cento', 'Elite', and 'Cannes'. Substrate-specific activity of DFR was observed because leucopelargonidin (LPg), which uses DHK as a substrate, was not detected in the tested cultivars.

• Journal of Plant Biotechnology
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• v.32 no.2
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• pp.123-128
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• 2005

A series of experiments were performed to establish regeneration system through friable embryogenic callus (FFC) of Lilium longiflorum 'Nellie White'. Only hard and regular callus was induced from bulb scales on medium containing 2.0 mg/L dicamba and $30{\sim}90$ g/L sucrose. The induced hard callus was subcultured on medium with 2.0 mg/L dicamba and 30 g/L sucrose, and used as a material for induction of FEC. In order to induce FEC, induced hard and regular callus was chopped into $1{\sim}2\;mm$ segments, and re-cultured on medium with 2.0 mg/L dicamba and 90 g/L sucrose. FEC was induced from chopped hard calli by the subcultures of two months interval. The induction rate of FEC was enhanced when hard callus was subcultured on same medium. FEC was proliferated more than 5 times on medium with $1.0{\sim}2.0\;mg/L$ dicamba and 90 g/L sucrose. Bulblet differentiation from FEC was very favorable on MS medium supplemented with 0.1 mg/L BA, 1.0 mg/L NAA and 30 g/L maltose, but many differentiated bulblets were changed to vitrificated ones. The differentiation of normal bulblets was most effective on medium containing $0.5{\sim}1.0\%$ activated charcoal and 30 g/L sucrose.