• Title/Summary/Keyword: L-Met

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Estimation of radionuclides leaching characteristics in different sized geopolymer waste forms with simulated spent ion-exchange resin

  • Younglim Shin;Byoungkwan Kim;Jaehyuk Kang;Hyun-min Ma;Wooyong Um
    • Nuclear Engineering and Technology
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    • v.55 no.10
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    • pp.3617-3627
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    • 2023
  • This study presents a method to solidify spent ion-exchange resin (IER) in a metakaolin-based geopolymer and shows results of mechanical strength, immersion, leaching, irradiation, and thermal cycling tests for waste acceptance criteria (WAC) to repository. The geopolymer waste form with 20 wt% of simulated spent IER met the WAC in South Korea (ROK), and the leaching tests of various sized-waste forms up to 15.0 × 30.0 cm and waste loadings up to 20 wt% for 1-5 d and 1-90 d achieved a leachability index, Li > 6. In a leaching test for 5 d, the cumulative fraction leached (CFL) for Cs, which leached the most, was linearly correlated with the square root of leaching time for all waste forms, and Li increased as the size of the waste form increased. The CFL was also correlated with elapsed time in the 90 d leaching test. The correlations among CFL, time, and volume-to-surface area ratio of waste forms used to estimate the Li of Cs of a 200-L sized geopolymer with 15 wt% IER showed the Li values as 14.73 (5 d) and 17.71 (90 d), respectively, indicating that the large-sized geopolymer waste form met the WAC.

Comparing Physical and Thermal Environments Using UAV Imagery and ENVI-met (UAV 영상과 ENVI-met 활용 물리적 환경과 열적 환경 비교)

  • Seounghyeon KIM;Kyunghun PARK;Bonggeun SONG
    • Journal of the Korean Association of Geographic Information Studies
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    • v.26 no.4
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    • pp.145-160
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    • 2023
  • The purpose of this study was to compare and analyze diurnal thermal environments using Unmanned Aerial Vehicles(UAV)-derived physical parameters(NDVI, SVF) and ENVI-met modeling. The research findings revealed significant correlations, with a significance level of 1%, between UAV-derived NDVI, SVF, and thermal environment elements such as S↑, S↓, L↓, L↑, Land Surface Temperature(LST), and Tmrt. In particular, NDVI showed a strong negative correlation with S↑, reaching a minimum of -0.52** at 12:00, and exhibited a positive correlation of 0.53** or higher with L↓ at all times. A significant negative correlation of -0.61** with LST was observed at 13:00, suggesting the high relevance of NDVI to long-wavelength radiation. Regarding SVF, the results showed a strong relationship with long-wave radiative flux, depending on the SVF range. These research findings offer an integrated approach to evaluating thermal comfort and microclimates in urban areas. Furthermore, they can be applied to understand the impact of urban design and landscape characteristics on pedestrian thermal comfort.

Effect of Moisture, Relative Humidity and pH on Color and Pigments of Cold Storage Beef Meat (수분함량, 상대습도 및 pH가 냉장 우육의 색도 및 색소에 미치는 영향)

  • 정인철;문귀임;김기영;김미숙;이교연;강세주
    • The Korean Journal of Food And Nutrition
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    • v.11 no.2
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    • pp.221-227
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    • 1998
  • This study was investigate correlation of moisture content, relative humidity, pH, color difference, myoglobin(Myo), oxymyoglobin(Oxy) and metmyoglobin(Met). The moisture content, relative humidity of loin and shank were not changed uniformly during storage, the pH was raised according to storage period. The L value was raised at the closing stage of the storage period, the surface of the b value was higher than interior. Between Myo and Met were positive correlation in common with loin and shank. The surface and interior of loin were negative correlation between Oxy and Met, the surface and interior shank were positive correlation between Myo and L value and were negative correlation between Myo and Oxy. The moisture content, relative humidity, pH and color was not correlation uniformly.

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CRYSTAL STRUCTURE OF tRNA ($m^1$ G37) METHYLTRANSFERASE

  • Ahn, Hyung-Jun;Lee, Byung-Ill;Yoon, Hye-Jin;Yang, Jin-Kuk;Suh, Se-Won
    • Proceedings of the Korea Crystallographic Association Conference
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    • 2003.05a
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    • pp.17-17
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    • 2003
  • tRNA (m¹ G37) methyltransferase (TrmD) catalyze s the trans for of a methyl group from S-adenosyl-L-methionine (AdoMet) to G/sup 37/ within a subset of bacterial tRNA species, which have a residue G at 36th position. The modified guanosine is adjacent to and 3' of the anticodon and is essential for the maintenance of the correct reading frame during translation. We have determined the first crystal structure of TrmD from Haemophilus influenzae, as a binary complex with either AdoMet or S-adenosyl-L-homocysteine (AdoHcy), as a ternary complex with AdoHcy/phosphate, and as an apo form. The structure indicates that TrmD functions as a dimer (Figure 1). It also suggests the binding mode of G/sup 36/G/sup 37/ in the active site of TrmD and catalytic mechanism. The N-terminal domain has a trefoil knot, in which AdoMet or AdoHcy is bound in a novel, bent conformation. The C-terminal domain shows a structural similarity to DNA binding domain of trp or tot repressor. We propose a plausible model for the TrmD₂-tRNA₂ complex, which provides insights into recognition of the general tRNA structure by TrmD (Figure 2).

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Development of AFLP and STS Markers Related to Stay Green Trait in Multi-Tillered Maize

  • Jang Cheol Seong;Lee Hee Bong;Seo Yong Weon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.4
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    • pp.358-362
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    • 2004
  • In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a $91\%$ homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a $96\%$ homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.

Meat Quality Comparison of Beef from Hanwoo Supplemented with Dietary Rhus verniciflua Stokes Meal, Silicate, and Chromium-Methionine during Refrigerated Storage (옻나무 분말, 규산염 및 크롬메티오닌을 급여한 한우육의 냉장저장 중 육질 비교)

  • Kang, Sun-Moon;Lee, Ik-Sun;Song, Young-Han;Lee, Sung-Ki
    • Food Science of Animal Resources
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    • v.28 no.2
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    • pp.138-145
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    • 2008
  • This study was carried out to compare the meat quality of beef from Hanwoo supplemented with dietary Rhus verniciflua Stokes (RVS) meal, silicate (Si), and chromium-methionine (Cr-Met) during refrigerated storage. The 26 mon-aged Hanwoo steers were fed basal diets containing 4% RVS, 4% RVS+400 ppm Cr-Met, 1.4% $SiO_2$, or 0.14% $SiO_2+400 ppm$ Cr-Met for 4 mon prior to slaughter. The M. longissimus from carcasses were then stored at $4{\pm}0.2^{\circ}C$ for 7 d. The crude fat content was higher in the Si and Si+Cr-Met groups (p<0.05). The water-holding capacity (WHC) and tenderness were highest in the Si+Cr-Met group (p<0.05). With regard to fatty acids, the polyunsaturated fatty acid (PUFA) content was lower in the Si and Si+Cr-Met groups (p<0.05), and the monounsaturated fatty acid (MUFA) content was lowest in the Si+Cr-Met group (p<0.05). The TBARS and MetMb contents were decreased in the Si, Si+Cr-Met, and RVS+Cr-Met groups. Regarding meat color during storage, the Si+Cr-Met group showed the highest L, a, b, C values and total color, and those of the Si and RVS+Cr-Met groups were higher than the RVS group (p<0.05). Consequently, beef from Si-fed Hanwoo had higher fat content, color and oxidation stability, and lower PUFA content than RVS-fed beef. And beef from Si+Cr-Met-fed Hanwoo had higher WHC, tenderness and color stability, and lower MUFA content than Si-fed beef.

Responses of Dairy Cows to Supplemental Highly Digestible Rumen Undegradable Protein and Rumen-protected Forms of Methionine

  • Sun, T.;Yu, X.;Li, S.L.;Dong, Y.X.;Zhang, H.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.5
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    • pp.659-666
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    • 2009
  • Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 193${\pm}$13 days in milk were used in a 4${\times}$4 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation ($NE_L$), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, $NH_3g-N$ and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.

Regional Integrity Analysis using modernized GPS, Galileo and SBAS

  • Han, Sang-Sul;Shin, Dae-Sik;Cho, Jong-Chul;Park, Chan-Sik;Jun, Hyang-Sik;Nam, Gi-Wook;Lee, Sang-Jeong
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • v.1
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    • pp.417-421
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    • 2006
  • ICAO defines performance requirements of navigation system such as accuracy, integrity, continuity and availability. The integrity is most significant performance requirement in aviation where safety of life is crucial. Many researches on this topic anticipate that GPS with SBAS or Galileo can meet APV requirements and GPS with GBAS or Galileo with GBAS will meet CAT II and III requirements. These performance expectations are based on global analysis. In this paper regional integrity analysis in Korea using various combinations of modernized GPS, Galileo and SBAS is given. The simulation results show that CAT I requirement can be met using modernized GPS and Galileo alone, however, CAT II and III are not met even augmenting SBAS because of VPL. A more efficient augmentation such as GBAS which can reduce VPL dramatically is required to meet CAT II and III in Korean region.

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AdoMet Derivatives Induce the Production of Actinorhodin in Streptomyces coelicolor

  • Lee Yu-Kyung;Young Jung-Mo;Kwon Hyung-Jin;Suh Joo-Won;Kim Jin-Young;Chong You-Hoon;Lim Yoong-Ho
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.965-968
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    • 2006
  • Exogenous S-adenosyl-L-methionine (AdoMet) enhances the production of actinorhodin in Streptomyces coelicolor. Thirty compounds related structurally with AdoMet were tested for their actinorhodin production. The relationships between the structures of the compounds tested and their actinorhodin production were analyzed using computational methods, and the molecules containing both bulky substituents at the C6 position of adenine and the long 5'-alkyl chain of adenosine were predicted to show high actinorhodin production.

Characterization of a novel methionine sulfoxide reductase A from tomato (Solanum lycopersicum), and its protecting role in Escherichia coli

  • Dai, Changbo;Singh, Naresh Kumar;Park, Myung-Ho
    • BMB Reports
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    • v.44 no.12
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    • pp.805-810
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    • 2011
  • Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and $K_m$ and $K_{cat}$ values for MSRA2 were observed as 8.5, $25^{\circ}C$, $352{\pm}25\;{\mu}M$, and $0.066{\pm}0.009\;S^{-1}$, respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage.