• Title/Summary/Keyword: L-77

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Induced Spawning of Striped Knife-Jaw, Oplegnathus fasciatus by Manipulating Water Temperature and Photoperiod (수온 및 광주기 조절에 의한 돌돔, Oplegnathus fasciatus의 산란 유도)

  • 정관식;김석민;방인철;김성연;이원교
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.141-149
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    • 1998
  • Spawning inducement of striped knife-jaw, Oplegnathus fasciathus, was attempted by two experiments. In experiment I (Exp. I), water temperature was gradually increased from 14.5$^{\circ}C$ in December 15, 1996 to $21.0^{\circ}C.$ in February 22, `997 and then maintained at this level. Photo-period was also gradually increased from 10.5L/13.5D in December 15, 1996 to 15.5L/8.5D in February 17, 1997, and then maintained at this level. In experiment II (Exp. II), water temperature was increased in the same way from Exp. I. Photoperiod was natural conditions from December 1996 to March 9, 1997, and then suddenly increased to 15.5L/8.5D until the end of experimental period. Spawning of the fish was occurred from February 22 through April 2, 1997 (for 40 days) in Exp. I. Number of total spawned eggs was 30.04 million and fertilization rate was 77.2%. The fish began to spawn at $21.0^{\circ}C.$ and 15.5L/8.5D. It requied 65 days to spawn since the water temperature had increased from $14.5^{\circ}C.$ to $21.0^{\circ}C.$. Spawning of the fish was not occurred until March 9, 1997 in Exp. II. After 7 days, photo-period was suddenly incresed to 15.5L/8.5D and fish were spawned from March 17 through April 4, 1997 (for 20 days). Number of total spawned eggs was 21.28 million and fertilization rate was 72.1%. The fish began to spawn at $21.0^{\circ}C$ and 15.5L/8.5D. It required 65 days to spawning since the water temperature had increased from $14.5^{\circ}C$ to $21.0^{\circ}C$.

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Physicochemical properties and anti-wrinkle effect of polysaccharides with different molecular weights from Gloiopeltis furcata (불등풀가사리 다당류의 분자량에 따른 이화학적 품질특성 및 피부 주름 개선 효과)

  • Lee, Dae-Hoon;Hong, Joo-Heon
    • Food Science and Preservation
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    • v.24 no.5
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    • pp.688-696
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    • 2017
  • In this study, the physicochemical properties and anti-wrinkle effect of polysaccharides with different molecular weights from Gloiopeltis furcata were investigated. Crude polysaccharides were isolated by viscozyme treatment followed by ethanol precipitation and lyophilization. Crude polysaccharides were hydrolyzed by acid (0.1 N HCl) and the molecular weight fractions were generated by centrifugal filter (<10 kDa, 10 to 100 kDa, and 100 kDa>). The yield of polysaccharides with different molecular weight fractions was 8.4-39.6%. The major constituents in molecular weight fractions were total sugar (81.37-85.82%), uronic acid (27.89-32.85 g/100 g), sulfate (33.38-39.04%), and protein (0.35-3.16%) The L, a, and b value of the 100 kDa group were decreased, but viscosity increased. The oxygen radical absorbance capacity of the 100 kDa group at $180.07{\mu}M$ was the highest among groups. The protective effects of 100 kDa group at 0.5 and $5{\mu}g/mL$ against $H_2O_2$-induced cytotoxicity in L132 cell were 87.34% and 103.85%, respectively. The matrix metalloproteinase-1 activity of 100 kDa group decreased in a dose-dependent manner. The pro-collagen synthesis activity of 100 kDa group at $0.05-0.5{\mu}g/mL$ was 64.91-77.80%. The polysaccharides with different molecular weights from Gloiopeltis furcata investigated herein are useful as a potential candidate for cosmedical materials.

Extracts of Rheum undulatum L. Inhibits COX-2 Activities in Lipopolysaccharide-stimulated Raw 264.7 Cells (종대황 추출물의 COX-2 활성 억제 효과)

  • 하혜경;이제현;김정숙
    • Biomolecules & Therapeutics
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    • v.8 no.1
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    • pp.73-77
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    • 2000
  • Rheum undulatum L. has been used as Rhei Radix in Korean Pharmacopea although their pharmacological effects were not studied much. In this studym, we tested anti-inflammatory effect as a representative activity of Rheum undulatum L. extracts using cyclooxygenase (COX)-2 inhibition. Murine macrophage, Raw 264.7 cells were incubated with lipopolysaccharide (1 $\mu\textrm{g}$/ml) to induce COX-2. The prostagladin $E_2$ ($PGE_2$) levels as an indicator of COX-2 activity were determined in the culture medium using ELISA. Inhibition of acetylsalicylic acid (ASA) as a standard, aloe-emodin, chrysophanol, rhein, 80% ethanol extract of Rheum undulatum L. (Ex) and ether fraction (Fr) after acid hydrolysis of Rheum undulatum L. were tested in induced COX-2 described above. $IC_{50}$ values were 0.082 $\mu\textrm{g}$/ml for ASA. 181 $\mu\textrm{g}$/ml for aloe-emodin, 3.65 $\mu\textrm{g}$/ml for emodin, 144 $\mu\textrm{g}$/ml for chrysophanol, 39.8 $\mu\textrm{g}$/ml for rhein, 141 $\mu\textrm{g}$ of herb/ml for Ex, and 95.7 $\mu\textrm{g}$ of herb/ml for Fr. We found that Ex and Fr of Rheum undulatum L. were more effective than other anthraquinones, since their $IC_{50}$ are lower than others.

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A Study of Risk Factor Analysis of Republic of Korea Air Force Training Pilot (공군 훈련 조종사 위험요인 분석에 관한 연구)

  • Hakbong Lee;Ohsung Kwon
    • Journal of the Korean Society for Aviation and Aeronautics
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    • v.31 no.3
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    • pp.1-9
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    • 2023
  • The overall Air Force's serious accident rate is decreasing, but the trainer's accident rate is on the rise from 0.24% in '00~'09 to 0.77% in '01~'19. Base on the SHELL model recommended by the ICAO, the risk factors of the introductory flight training course were analyzed, implications were examined, and safety promotion measures were proposed. A survey of 25 questions was organized based on regulations, guidelines, and related data for each component of the SHELL model in accordance with the introductory course of Air Force flight training. The survey was divided into a student group and instructor group and compared and analyzed into L-L, L-S, L-H, and L-E based on the results after conducting it, and implications for this were derived. Compared to other analysis factors, L-L showed that the average of the instructor group was relatively higher that of the student group in all questions.

Implementation of Distributed Feedback Filters using Cascaded Gratings with Different Period (주기가 다른 격자들로 구성된 DFB 필터의 구현)

  • Ho, Kwang-Chun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.13 no.1
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    • pp.77-82
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    • 2013
  • The filtering characteristics of planar distributed feedback (DFB) waveguides composed by gratings with different period are solved using equivalent transmission-line network. To analyze explicitly its band-pass and resonance properties, a longitudinal modal transmission-line theory (L-MTLT) based on Floquet's theorem and Babinet's principle is newly developed. The numerical results reveal that this approach offers a simple and analytic algorithm to analyze the filtering characteristics of cascaded DFB structure with different period, and the bandwidth and side-lobe suppression of cascaded DFB filter are sensitively dependent on the variation of aspect ratio and the number of grating at each region.

Influence of subclinical hypothyroidism on metabolic parameters in obese children and adolescents

  • Kara, Ozlem
    • Clinical and Experimental Pediatrics
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    • v.63 no.3
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    • pp.110-114
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    • 2020
  • Background: Subclinical hypothyroidism (SH) is a common condition in obese children. However, its effect on glucose and lipid metabolism in obese children remains controversial. Purpose: The present study aimed to investigate the association between SH and metabolic parameters. Methods: A total of 215 obese children and adolescents aged 6-18 years were included in this retrospective cross-sectional study. The patients' anthropometric measurements such as thyrotropin (TSH), free thyroxine (fT4), fasting plasma glucose, and insulin levels, as well as homeostasis model assessment for insulin resistance (HOMA-IR) index, and lipid profiles were evaluated. The patients were allocated to the SH group (fT4 normal, TSH 5-10 mIU/L) (n=77) or the control group (fT4 normal, TSH<5 mIU/L) (n=138). The glucose and lipid metabolisms of the 2 groups were compared. Results: SH was identified in 77 of 215 patients (36%). Mean body mass index was similar in both groups. The mean serum insulin, HOMA-IR, and triglyceride (TG) levels were higher and the mean high-density lipoprotein cholesterol level was lower in the SH group than in the control group (P=0.007, P=0.004, P=0.01, and P=0.02, respectively). A positive correlation was observed between TSH level and insulin level, HOMA-IR, and TG level. Conclusion: SH was identified in some of the obese children and adolescents. A clear association was observed between SH, insulin resistance, and dyslipidemia in obese children.

A Study on the Weld Part Fracture Toughness of Austenite Type Stainless Steel for Cryogenic Liquid Nitrogen Storage Tank (초저온 액화질소 저장탱크 오스트나이트계 스테인리스강의 용접부의 파괴인성 연구)

  • Kim, Young-Deuk;Choi, Dong-Jun;Park, Hyung-Wook;Cho, Jong-Rae;Bae, Won-Byoung
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.6
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    • pp.802-808
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    • 2011
  • One of the important mechanical properties of cryogenic temperature structure material is fracture toughness. Research on normalization of fracture toughness test method is becoming very important issue with development of cryogenic structural elements. Specially, mechanical properties estimation by each micro-structure of welding department is important because it can cause unstable fracture when use under cryogenic environment in case of welding department. In this study, fracture toughness estimation test was carried out to unloading compliance method and sensitization heat-tread minimized test specimen at liquid nitrogen (77K), liquid helium (4K), 293K temperature to STS-316L base metal and weld metal.

An efficient transformation method for a potato (Solanum tuberosum L. var. Atlantic)

  • Han, Eun-Hee;Goo, Young-Min;Lee, Min-Kyung;Lee, Shin-Woo
    • Journal of Plant Biotechnology
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    • v.42 no.2
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    • pp.77-82
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    • 2015
  • We found that a long period of in vitro culture is a critical factor on the low transformation rate for a specific potato genotype, Solanum tuberosum L. var. Atlantic when phosphinothricin (PPT) was added to select putative transformants in a solid media. The fresh explants of the newly produced plants from a micro-tuber was able to increase the transformation rate significantly while the old explants prepared from a plant maintained for longer than 6 months in vitro by sub-culturing every 3 ~ 4 weeks resulted in a very low transformation frequency. However, Jowon cultivar was not so much influenced by the period of in vitro culture with high transformation rate (higher than 10.0%). Further research need to be explored for the reason why a particular potato genotype, Atlantic is more vulnerable than the Jowon cultivar during the regeneration stage resulting in the low transformation frequency.

Evaluation of a Thermophilic Two-Phase Anaerobic Digestion Coupled with Membrane Process for Garbage Leachate Treatment (음식물 탈리액 처리를 위한 막결합형 고온 2상 혐기성 소화 공정의 평가)

  • Lee, Eun-Young;Jun, Duk-Woo;Lee, Sang-Hwa;Bae, Jae-Ho;Kim, Jeong-Hwan;Kim, Young-O
    • Journal of Korean Society of Water and Wastewater
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    • v.26 no.1
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    • pp.21-27
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    • 2012
  • This study evaluated the performance of a thermophilic two-phase anaerobic digestion (TTPAD) coupled with membrane process treating garbage leachate. The pilot-scale treatment system is consisted of thermophilic acidogenic reactor (TAR) and thermophilic methanogenic reactor (TMR) coupled with an ultrafiltration (UF) membrane unit. The hydraulic retention time of TAR and TMR were 4 and 20 days, respectively. Effluent TCOD and SCOD of the TTPAD were $25\;{\pm}\;6\;and\;12\;{\pm}\;3$ g/L, respectively, and the corresponding TCOD and SCOD removal efficiencies were 77% and 81%, respectively. Propionate was major acids as 75% in the effluent. Scum formation was not observed in TTPAD, which might be resulted from complete lipid degradation. However, TTPAD was appeared to be sensitive to free ammonia toxicity. The UF membrane was operated with constant pressure filtration at average TMP 1.3 atm. Permeate flux had a range of 15-30 $L/m^2/hr$. With UF membrane, TCOD removal increased from 77% to 93%, and this SS free effluent would be beneficial to subsequent processes such as ammonia stripping.

Analysis of Minerals, Amino Acids,and Vitamin of Lespedeza cuneata (비수리의 미네랄, 이미노산, 비타민 분석)

  • Ding, Ji-Lu;Lim, Ik-Jae;Lee, Hee-Duck;Cha, Wol-Suk
    • KSBB Journal
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    • v.21 no.6 s.101
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    • pp.414-417
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    • 2006
  • For developing functional biomaterials, chemical analysis of Lespedeza cuneata including minerals, amino acids and vitamin were investigated. Minerals of L. cuneata were found to be calcium, potassium, magnesium, phosphorus, sodium, iron, manganese, zinc and copper. Among the free amino acid, proline was 33.77 mg% that were 67.2% of free amino acids and essential amino acids were 7.49 mg%. Total amino acids were analyzed as 2,817 mg% and the content of glutamic acid (496.00 mg%) was highest. In case of vitamin, the highest components was vitamin E with 33.03 mg%.