• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.79.1-79
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• 2003

Two pathogen-induced hot pepper transcription factors (CaNACl and CapIfl) were introduced into‘MicroTom’tomato by Agrobacterium tumefaciens-mediated transformation. We used to nptII containing kanamycin resistance gene as a selection marker. Both transformed and non-transformed plants were transferred to pot after rooting test in vitro. To approximate the levels of caNACl transcript in leaves of wild-type and transgenic plants, RNA blots were hybridized with double-stranded full-length CaNACl probe at moderate stringency, Although the relative signal strength for hybridization fluctuated among the samples on different blots, transgenic plant lines N-1, N-2 and N-3 consistently displayed increased levels of CaNACl transcript relative to other transgenic lines and wild-type plants. Of all the transgenic lines examined, line N-7 had the least amount of CaNACl transcript. Role of these transcription factors in pathogen defense will be examined by overexpression in tomato.

• 한국자원식물학회지
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• 제15권1호
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• pp.8-17
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• 2002

새 번식방법 개발을 위해 둥굴레 뿌리는 눈이 없어도 부정아가 발생된다는 점에 착안하여 뿌리길이와 굵기에 대한 부정아 발생에 관한 시험 연구를 한 결과는 다음과 같다. 1년차는 뿌리길이가 5cm 경우 66.5%지만 15cm에서는 113% 부정아 발생되었고, 뿌리의 굵기 5mm이하는 98.3%지만 10mm이상은 113.3%로 46.7% 차이를 보였다. 2년차는 부정아 생성률은 15cm에서는 1년차의 3배, 시험구 설치 당시 전혀 눈이 없는 상태에서 4배의 증식을 보였다. 용토는 일반토양 2.6배 보다 모래나 펄라이트가 3.1~3.3배 증식 되었다. 대량번식을 위한 가장 좋은 방법은 10mm 둥굴레 뿌리를 5cm로 절단하여 모래에 식재하는 방법으로 판단된다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.100.2-101
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• 2003

The global regulator, GacS (global antibiotic and cyanide sensor kinase), was required for the increased resistance to hydrogen peroxide occurring as cultures of the rhizobacterium, P. chlororaphis O6, matured. Specific stationary-phase peroxidase and catalase isozymes were absent in the GacS mutant, whereas a manganese-superoxide dismutase isozyme was expressed earlier and to a great extent than wild type. In the wild type cell, transcript accumulation of rpoS was higher in late logarithmic-phase cells than cells from mid logarithmic- or stationary-phase. Transcripts from rpoS in the GacS mutant were reduced in each of these growth phases compared to the wild type expression. The down stream sequence from rpoS lacked sequences encoding a small RNA, rsmZ, found in other pseudomonads and implicated in control of genes activated by the GacS system. These findings suggest that GacS-mediated regulation of RpoS plays role in control of oxidative stress in P. chlororaphis O6 by as yet an unknown mechanism.

• Journal of Ginseng Research
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• 제44권4호
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• pp.637-643
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• 2020

Background: Ginseng (Panax ginseng Meyer) is one of the world's most valuable medicinal plants with numerous pharmacological effects. Ginseng has been cultivated from wild mountain ginseng collections for a few hundred years. However, the genetic diversity of cultivated and wild ginseng populations is not fully understood. Methods: We developed 92 polymorphic microsatellite markers based on whole-genome sequence data. We selected five markers that represent clear allele diversity for each of their corresponding loci to elucidate genetic diversity. These markers were applied to 147 individual plants, including cultivars, breeding lines, and wild populations in Korea and neighboring countries. Results: Most of the 92 markers displayed multiple-band patterns, resulting from genome duplication, which causes confusion in interpretation of their target locus. The five high-resolution markers revealed 3 to 8 alleles from each single locus. The proportion of heterozygosity (H_e) ranged from 0.027 to 0.190, with an average of 0.132, which is notably lower than that of previous studies. Polymorphism information content of the markers ranged from 0.199 to 0.701, with an average of 0.454. There was no statistically significant difference in genetic diversity between cultivated and wild ginseng groups, and they showed intermingled positioning in the phylogenetic relationship. Conclusion: Ginseng has a relatively high level of genetic diversity, and cultivated and wild groups have similar levels of genetic diversity. Collectively, our data demonstrate that current breeding populations have abundant genetic diversity for breeding of elite ginseng cultivars.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.247-247
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• 2022

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.98-98
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• 2022

• 한국자원식물학회지
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• 제2권2호
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• pp.259-278
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• 1989

입암산 지역에 분포되어 있는 한약자원식물을 채집 분류하여 이용체계 확립의 수단으로 삼고자, 이 지역의 약용식물의 종류를 조사한 결과는 다음과 같았다. 1. 입암산에 분포되어 있는 한약자원 식물은 100 과, 252 속, 337 종이었다. 2. 분석수종중 초본류는 59 과, 169 속, 220 종, 목본류는 50 과, 85 속, 117 종이었다. 3. 초본류는 주으로 전초를 사용하며 수본류는 기, 수피 및 과실을 주로 약용으로 이용하는 것이 많았다.

• 한국환경과학회지
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• 제13권12호
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• pp.1009-1013
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• 2004

The purpose of this study is to select the appropriate wild herbaceous plants on the rooftop. For the purpose of the experiments, 14 wild herbaceous plants were chosen. As the results of the experiments, the wild herbaceous plants growing on the culture soil(perlite) Among the 14 kinds of wild herbaceous plants, such as Chrysanthemum boreale, Taraxacum mongolicum, Aster Koraiensis, Aster yomena, Oenothera odorata, Oenothera lamarckiana, Patrinia scabiosaefolia showed good effects on growth of above ground parts. Therefore, these plants will effective for the rooftop revegetation. Callistephus chinensis and Lotus corniculatus var. Japonicus showed good height growth, wherase covering rate was worst. Chrysanthemum boreale, Platycodon grandiflorum, Patrinia scabiosaefolia were plant height, wherase covering rate was not good . For the expected number of seedings more than 3,000 per square meter, many seedings got withered to death while the survivors were suppressed to grow slow in the early stage due to the densityproblem.

• BMB Reports
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• 제41권7호
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• pp.542-547
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• 2008

D-type cyclins control the onset of cell division and the response to extracellular signals during the G1 phase. In this study, we transformed a D-type cyclin gene, Nicta;CycD3;4, from Nicotiana tabacum using an Agrobacterium-mediated method. A predicted 1.1 kb cyclin gene was present in all of the transgenic plants, but not in wild-type. Northern analyses showed that the expression level of the Nicta;CycD3;4 gene in all of the transgenic plants was strong when compared to the wild-type plants, suggesting that Nicta;CycD3;4 gene driven by the CaMV 35S promoter was being overexpressed. Our results revealed that transgenic plants overexpressing Nicta;CycD3;4 had an accelerated growth rate when compared to wild-type plants, and that the transgenic plants exhibited a smaller cell size and a decreased cell population in young leaves when compared to wild-type plants.

• 미생물학회지
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• 제25권4호
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• pp.290-296
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• 1987

Some streptomycin resistant strains of Rhizobium trifolii having nodulation ability were selected, and their nitrogenase activities, symbiotic effects on plant growth, and nodule electronmicroscope were compared with those of the wild type. After NTG treatment, as a mutagen, at the concentration exhibiting 99.7% lethal rate, 5 strains of streptomycin resistant mutant having nodulating ability were selected. Among these nodulating mutant strains, 3 strains produced more nodules and 2 strains showed less nodules than wild type. But their nitrogenase activities were decreased significantly, and nodule formation time was also delay compared with those of the wild type, and there was no remarkable difference in effects on plant growth. Microstructure of nodules by electronmicroscopy had mant distinctive differences between red clover nodules inoculated with wild type and mutants.