• Journal of Dairy Science and Biotechnology
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• v.23 no.2
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• pp.149-153
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• 2005

Lactic acid bacteria had been widely utilized in fermented foods such as fermented dairy products, traditional sauces, kimchi, fermented sausages, medicines or probiotic feed additives for a long time. LAB are also widely distributed in the mammalian gastrointestinal tracts, oral cavity, vagina, and various foods or soils. The most familiar examples of using LAB would be the fermented milk products, and those had become one of the favorite foods in Korea f3r more than 34 years of history. The main benefits of the fermented milk products were originally the improvement of the balance of intestinal flora to control diarrhea and congestion disorders, and gradually, they were chosen by the consumer preference of the taste. The very beginning of the fermented milk products in Korea was the Yakult type products in 1971, and it was the commencement of the solid foundation of the dairy industry and the understanding beneficial effect of probiotic yogurt. After middle of 80's, stirred type fermented milk products had been firstly produced, and it was the time that the domestic dairy industry took root in Korea. From 90's, functional fermented milk products were produced, and drink type yogurt sales a mount increased drastically, and these products began to be chosen not only with the values of nutrition but also with physiological functions. The health claims are classified into intestinal health, gastric health and hepatic health. The prospects for the Korean market are as follows; The majority of leading products would be the premium functional yogurt products as it was last year. It is because the sales of lower cost products shows slow-down, and industries tend to increase the commercial advertisements of premium functional yogurt products through mass media. These tendencies would make the market situation become more competitive.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.3
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• pp.599-607
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• 2009

Prunellae Spica is the spike or whole plant of Prunella vulgaris Linne, which has been used for clearing heat from the liver, brightening the eyes and treating headache in traditional oriental medicines. This study was conducted to evaluate the inhibitory effects of the aqueous extract of Prunellae Spica (PSE; PS extract) on the production of NO and PGE2 in LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay, and all three doses of PS extract (0.03, 0.1 and 0.3 mg/ml) had no significant cytotoxicity during the entire experimental period. The cells were treated with 1 ${\mu}g/ml$ of LPS 1 h before adding PS extract, and increased NO and PGE2 production were detected in LPS-activated cells compared to control. However, these increases were dose-dependently attenuated by treatment with PS extract. The inhibition of NO by PS extract was due to the suppression of iNOS expression via inhibition of $NF{\kappa}B$ nuclear translocation and proteolytic degradation of $I{\kappa}B{\alpha}$. The decreased level of PGE2 was derived from inhibition of COX-2 activity, but expression of COX-2 protein was not affected by PS extract. Moreover, PS extract reduced the elevated production of IL-${\beta}$ and IL-6 by LPS. These results demonstrate that PS extract has inhibitory effects on the production of NO and PGE2 as a consequence of the reduction of proinflammatory cytokines, especially IL-${\beta}$ and IL-6 in LPS-activated Raw 264.7 cells.

• The Korea Journal of Herbology
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• v.32 no.1
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• pp.55-62
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• 2017

Objectives : Crataegi Fructus has been used as traditional medicines for more digestion action, amenorrhea due to blood stasis, and hyperlipemia. The aim of this study was to compare of Crataegi Fructus in South Korea collected during three years according to the standards in monographs of the Korean Pharmacopoeia Eleventh edition (KP 11). Methods : Crataegi Fructus was carried out identification test (Qualitative reaction, Thin layer chromatography), heavy metal test, and total ash registered at KP. Add to we tested loss on dry, contents of ethanol-soluble extracts, and HPLC profiling. Results : Identification test (TLC) was on comparing with ursolic acid standard solution in $R_f$ value, all samples showed red purple spot ($R_f$ value 0.9). Ursolic acid spot in $R_f$ value 0.35 showed by changing mobile phase condition. Heavy metals showed contents for Pb, Cd, As, and Hg range of 0.0 ~ 0.5 ppm, 0.0 ~ 0.2 ppm, 0.0 ~ 0.3 ppm, and 0.0 ~ 0.1 ppm. Loss on drying was ranged from 5.5 to 11.9 %, total ash was between the range 2.7 ~ 4.0 %. Contents of ethanol-soluble extracts was ranged from 17.8 to 44.9 %. The content of chlorogenic acid was ranged from 0.0 to 0.1 % based on the chlorogenic acid standard curve. Conclusion : We have verified the current specification standard of Crataegi Fructus and standard that is not set. We hope that it will help the standardization of Crataegi Fructus.

• Korean Journal of Pharmacognosy
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• v.44 no.3
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• pp.291-297
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• 2013

Sophorae Flos (SF), a composite of flowers and flower-buds of Sophora japonica, has long been used in traditional Korean and Chinese medicines for the treatment of hemostasis and inflammation. In this study, we investigated anti-inflammatory effect of four EtOH extracts at the difference in blooming stages of flowers on LPS-induced inflammation in RAW264.7 cells. We classified the flowers of Sophora japonica with SF-1 (length of flower is shorter than calyx), SF-2 (length of calyx is shorter than flower), SF-3 (full bloom), and SF-4 (not blooming at all). We examined HPLC analysis, whether quercetin and rutin are major component of these Sophorae Flos extracts or not. As a result, SF-1 contained quercetin, but the others did not. In addition, quercetin, SF-1, and SF-4 act on the suppression of pro-inflammatory mediators including inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, nitric oxide (NO), prostaglandin E2 ($PGE_2$) against lipopolysaccharide (LPS)-induced activation in RAW264.7 cells. Of these, SF-1 showed the best anti-inflammatory effect. These results suggest that Sophorae Flos with the highest content of quercetin would be used for the treatment of various inflammation diseases.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.2
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• pp.358-362
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• 2005

Directional words like 'ascending(升, sheng)', 'descending(降, jiang)', 'floating(浮, fu)', 'sinking(沈, chen) are technical terminologies of Oriental Medicine to express the directions of material medica functioning-at the phase of Qi-superiorly, inferiorly, exteriorly, and interiorly in human body, and are constituting one of the major principles in prescribing medicines. Lacking the objective methodology to measure or, at least determine Qi, However, we can not expect to secure meaningful data to verify those directional terms. This problematic situation caused chaotic discrepancy between theory and practice of Oriental Medicine. Thus, adopting the method of dynamic meditation 'Hanalmomjit' of Lux Vitae meditation society, by which various state of Qi-sensational movements can be expressed, we compared its moves with the directional terms of traditional canons in Oriental Medicine. In this thesis, the experiment of expressing materia medica via 'Hanalmomjit' is resulted out and compared with the terms, confirmed to have a certain meaningfulness. The new method showed these results below: Herba Menthae, described to have the disposition of ascending and floating, showed 60% of ascending movements in 'Hanalmomjit' experiment. Radix Rehmanniae Praeparata, described to have the disposition of descending and sinking, showed 50% of descending movements. Radix Glycyrrhizae, described to have plain disposition, showed 60% of central movements. Rhizoma Atractylodis and Pericarpium Citri Reticulatae, described to have the disposition of ascending and floating, showed also ascending movements. Cortex Magnoliae Officinalis, described to have the disposition of descending and sinking, showed also descending movements. In conclusion, qi-sensational movement of 'Hanalmomjit' for materia medica showed sufficient relations described in classics, proving itself to be useful method for objective demonstration of material medica.

• Journal of Korean Medical classics
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• v.21 no.1
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• pp.79-92
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• 2008

Objective The analysis method based on the herbal formula's effects is a general tool, in traditional medicine. In effective applications of herbal formula, Korean herbal medicines traditionally used the classification methods based on the curative effects through the various compositions of herbal formulas. However' the effects of herbal formulas were not filed systemically in ancient literatures, and the standards to confirm their effects are not clear. Thus, it is not easy to classify herbal formulas according to the curative effects. Furthermore, there are no standards to estimate the effects of prescriptions frequently directed in clinic. In this study, we aimed to provide the methodology of classifying the curative effects of herbal formulas by calculating the combination of the effect of each compositional herb through the DB systems. Results : By comparing effects of herbal formula with those of compositional herbs, we found that about 25-50% of the herbal effects were included in herbal formula's effects. These results showed that the prospective estimation of herbal formula's effects may be possible through the DB systems filing herbal effects. To enhance the accuracy in explaining the herbal formula's effect, more studies are needed by giving prominence to major effects and by subtracting minor effects.

• Herbal Formula Science
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• v.27 no.3
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• pp.189-197
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• 2019

Objectives : The purpose of this study was to confirm whether Codonopsis Radix(CR) could be used in the same way for expected indications or diseases of adaptation instead of Ginseng Radix(GR), which acts as a principal herb in Sagunja-tang. Methods : The Traditional Chinese Medicine Systems pharmacology(TCMSP), a database for the study of systems biology related to Chinese medicine, screened potential active compounds in each quartet. By searching for all the proteins that each compound provides, the target of Sagunja-tang with GR(GRST) and the target of Sagunja-tang with CR(CRST) were compared using the network analysis method, and the top ranked target of each serving was selected. Results : Through TCMSP, a Chinese medicine database, the potential effective ingredients of GRST or CRST screened, and the target proteins related to these substances were found to be the most affected by Glycyrrhizae Radix et Rhizome, an herbal medicine mixed in Sagunja-tang, and the target diseases were the same. And the same were found for the target protein, gene and target diseases of GRST and CRST. Conclusions : The prescription with similar composition is likely to have similar network pharmacology analysis results, and the analysis result may be controlled by the herbal medicines which are assumed to be the main function. Therefore, rich and reproducible basic studies is more important because network pharmacological studies can be dominated by data that has been done a lot of previous studies.

• The Korea Journal of Herbology
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• v.32 no.2
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• pp.97-105
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• 2017

Objectives : Gardeniae Fructus is a ripe fruit of Gardenia jasminoides Ellis, which has been used as traditional medicines for anti-inflammatory, diuretic, antipyretic, and antibacterial activity. The aim of this study was to compare of Gardeniae Fructus in South Korea collected during three years according to the standards in monographs of the Korean Pharmacopoeia Eleventh edition (KP11). Methods : 30 items of Gardeniae Fructus from two cultivation regions were classified into dried(n=15) & steamed (n=15) and tested according to the standards in monographs of the KP11. Gardeniae Fructus was carried out identification(comparison of colors, thin layer chromatography), heavy metals, residual pesticides, total ash, and assay registered at KP11. Add to we tested loss on dry, contents of ethanol-soluble extracts, and HPLC profiling. Results : In TLC chromatogram of identification test, the spot of gardenoside and geniposide were observed at $R_f$ value of about 0.3 and 0.5. Heavy metals and residual pesticides met the requirements of the standards for all samples. The results of total ash of each samples are measured maximum 4.87 %. According to HPLC for assay, the samples contain 4.80~6.10 % of geniposide and 0.45~1.83 % of gardenoside. Conclusion : We have verified the current specification standard of Gardeniae Fructus and standard that is not set. By the results, it is proposed a new draft of loss on drying and confirmed the content of gardenoside revised. HPLC standard chromatogram of Gardeniae Fructus is proposed. We hope that it will help the standardization of Gardeniae Fructus.

• The Korea Journal of Herbology
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• v.34 no.4
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• pp.1-8
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• 2019

Objectives : Rosae Multiflorae Fructus is a traditional medicine derived from the fruit of Rosa multiflora Thunb. a member of the Rosaceae family. Even though it has a single origin, the possibility of adulterants has always existed. In fact, we had discovered suspicious commercial samples of Rosae Multiflorae Fructus, imported from China. Methods : To define the taxonomic origin of Rosae Multiflorae Fructus and its adulterants, DNA barcode analysis of the internal transcribed spacer, trnL-F intergenic spacer, and psbA-trnH sequences was carried out. These DNA barcode sequences from the correct origin of Rosae Multiflorae Fructus were analyzed and compared with those of other samples from genus Rosa used as medicinal herbs. Results : The analyses of the three DNA barcode sequences efficiently distinguished Rosae Multiflorae Fructus from six other species in genus Rosa and also separated each species used in this study. According to the DNA barcoding results, none of the suspicious commercial samples were Rosae Multiflorae Fructus. RMF09 was identified as Rosa acicularis, whereas RMF10 and RMF11 were identified as Rosa davurica and Rosa rugosa, respectively. These results corroborated the existence of adulterants of Rosae Multiflorae Fructus. Conclusions : Our research provides useful information that could be used as a criterion for distinguishing between Rosae Multiflorae Fructus and its adulterants. These results will help in the prevention of adulteration and also suggest effective methods for verifying the origin of commercial herbal medicines derived from genus Rosa.

• The Journal of Korean Medical History
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• v.35 no.1
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• pp.135-147
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• 2022

Sin Dae-woo was a scholar representing the Ganghwa School in the late Joseon Dynasty. He was Jeong Je-du's grandson-in-law and also in charge of organizing Jeong Je-du's collection of writings. His three sons had a particularly close relationship with their father, so even when they published a collection of writings after his father's death, the names of the three were combined and marked as Jin-jak-shin. The records they left include Seokcheon Il-seung, who described the history of the family along with the collection of writings, Seongdo Il-rok, a diary of the time of Seongcheon Busa, and many letters exchanged over time. These records draw attention as they contain records of medical life, such as eye diseases that the family suffered, infectious diseases that caused many casualties, and Yakro (distilled herbal essence) that showed records of being used for treatment at the time. In this paper: 1) We examine the medicines, prescriptions, acupuncture, and medication used to treat eye diseases. 2) We carefully examine the epidemic, the responses of the parties to it, and the attitudes of those who stood at the boundary between death and life. 3) We look for clues to the use of Western-origin drugs called Yakro. Intellectuals of the time looked deeper into Lee Kyugyung's book and Seo Yu-gu's book on how they were reflected in Joseon's medical life. In conclusion, in the 18th and 9th centuries, we see that the influential families of the Ganghwa school freely brought in famous acupuncturists, used prescriptions that were included in medical books or not, and used Yakro from western origin that were not traditional methods of Joseon. Thus, we reveal that doctors of the Joseon had the capacity to pursue their medical life more actively and had open-minded exchanges than our existing perceptions.