The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Quality Monitoring of Specification of Crataegi Fructus in the Korean Pharmacopoeia and Studies HPLC Standard Chromatogram

산사(山楂)의 규격 기준 모니터링 및 HPLC 표준크로마토그램 연구

  • Kim, Kyoung Hee (Korea Pharmaceutical Test & Research Institute) ;
  • Kim, Sun Mi (Korea Pharmaceutical Test & Research Institute) ;
  • Lee, Young Jong (Department of Herbology, College of Korean Medicine, Gachon University) ;
  • Baek, Wan Sook (Korea Pharmaceutical Test & Research Institute)
  • 김경희 (한국의약품시험연구원) ;
  • 김선미 (한국의약품시험연구원) ;
  • 이영종 (가천대학교 한의과대학 본초학교실) ;
  • 백완숙 (한국의약품시험연구원)
  • Received : 2016.12.10
  • Accepted : 2017.01.15
  • Published : 2017.01.30
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Abstract

Objectives : Crataegi Fructus has been used as traditional medicines for more digestion action, amenorrhea due to blood stasis, and hyperlipemia. The aim of this study was to compare of Crataegi Fructus in South Korea collected during three years according to the standards in monographs of the Korean Pharmacopoeia Eleventh edition (KP 11). Methods : Crataegi Fructus was carried out identification test (Qualitative reaction, Thin layer chromatography), heavy metal test, and total ash registered at KP. Add to we tested loss on dry, contents of ethanol-soluble extracts, and HPLC profiling. Results : Identification test (TLC) was on comparing with ursolic acid standard solution in $R_f$ value, all samples showed red purple spot ($R_f$ value 0.9). Ursolic acid spot in $R_f$ value 0.35 showed by changing mobile phase condition. Heavy metals showed contents for Pb, Cd, As, and Hg range of 0.0 ~ 0.5 ppm, 0.0 ~ 0.2 ppm, 0.0 ~ 0.3 ppm, and 0.0 ~ 0.1 ppm. Loss on drying was ranged from 5.5 to 11.9 %, total ash was between the range 2.7 ~ 4.0 %. Contents of ethanol-soluble extracts was ranged from 17.8 to 44.9 %. The content of chlorogenic acid was ranged from 0.0 to 0.1 % based on the chlorogenic acid standard curve. Conclusion : We have verified the current specification standard of Crataegi Fructus and standard that is not set. We hope that it will help the standardization of Crataegi Fructus.

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