• 대한의생명과학회지
• /
• 제12권4호
• /
• pp.427-433
• /
• 2006

We investigated the effect of propolis on the activity of antioxidant enzymes in rat liver exposed by X-ray irradiation. The dosage of propolis showed the effect of lowering the concentration of superoxide anion in irradiated rat liver, suggesting that propolis has a significant role to remove superoxide anion as an antioxidant and/or by activating the antioxidant enzyme. The activities of superoxide dismutase (SOD) and glutathione reductase (GR), disturbed by X-ray irradiation, were restored in 30 days to normal status in the group which dosed propolis before X-ray irradiation. Interestingly, catalase (CAT) and glutathione peroxidase (GPOX) activities were highly increased with feeding propolis to rat compared to untreated group, whereas glutathione s-transferase (GST) activity was little affected. Taken together, it suggests that the propolis has a protective role in the rat liver cells against X-ray irradiation by increasing and recovering the activities of antioxidant enzymes.

• 동아시아식생활학회지
• /
• 제15권5
• /
• pp.542-548
• /
• 2005

In recent years, propolis has attracted much attention as an useful substance in medicine and functional food, even if it is known as a natural remedy in folk medicine since ancient times. propolis was registered as natural food since 1995 on Korean Food Act by Korean Food and Drug Administration(KFDA). The present study demonstrated the optimization of isolation of crude propolis by ethanol, and tumoricidal effect of pro polis. The optimal concentration of ethanol to separate a high quantity of propolis was $60\%$. The cytotoxic effect of ethanol extracted propolis against various cancer cell lines including murine lymphoma (Sarcoma-180), murine T-lymphoma (YAC-1), human breast carcinoma (MCF-7), human gastric carcinoma (KATOIII) and human hepatocellular carcinoma (Hep3B) and human lung adenocarcinoma (A-549) was observed using SRB and MIT assay. In order to investigate the curative activity by oral administration of propolis on tumor, ICR mice was subcutaneously implanted Sarcoma 180. In 300mg/kg and 600mg/kg propolis administered group, development of implanted tumors was inhibited by $40.9\%\;and\;67.9\%$ at 16th day, respectively. In the same dose of propolis administered group, development of implanted tumors was inhibited more strongly with dose dependent manner. Therefore, these data suggested propolis may show tumoricidal effects. In conclusion, these results indicate that propolis, one of the few natural remedies, can be used as functional food with tumoricidal effects.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제39권2호
• /
• pp.82-85
• /
• 2019

We investigated the anti-microbial activity of propolis against the pathogenic bacteria and fungi on ginseng. We selected six microbials that caused postharvest root rots in ginseng. Propolis extracts were prepared by using the ethanol extraction method. We seeded the bacteria and fungi related to ginseng disease on a specific culture medium, and treated it with propolis extracts by using the paper disc method. Propolis extracts indicate the anti-microbial activity against Paenibacillus polymyxa, Fusarium solani, Rhizoctonia solani AG-1 and Pythium ultimum. However, the anti-fungal activity of propolis is weak on Pseudomonas fluorescens subsp. Cellulosa and Colletotrichum gloeosporioides. As a result, the antimicrobial effects of propolis against microbial that prevent ginseng growth were confirmed. The antimicrobial effects are shown according to the concentration of propolis against root rot. The fungi also showed antibacterial effects in a dose-dependent manner.

• Journal of Nutrition and Health
• /
• 제33권1호
• /
• pp.80-85
• /
• 2000

This study was undertaken to investigate the inhibitory effects of propolis on the in vitro proliferation of human colon(HT-29) and hepatoma(HepG2) cancer cell lines. The growth of the HT-29 and HepG2 cells was respectively inhibited by the administration of propolis in a concentration response-dependent manner. The distributions of HT-29 and HepG2 cells cultured in the medium containing propolis were shifted to the smaller sizes, and then HT-29 and HepG2 cells were shrunken under microscopic observations. The progression of cell cycle from G1 to S phase was significantly inhibited by propolis in the HT-29 and HepG2 cell lines, respectively. Those observations suggest that propolis has anticancer effect against some of cancer cell lines in vitro. (Korean J Nutrition 33(1) : 80-85, 2000)

• 한국동물위생학회지
• /
• 제42권4호
• /
• pp.305-311
• /
• 2019

The objective of this study was to determine the safety test for propolis in Beagle based on blood count, serum biochemistry, and electrolyte. Total six beagle dogs were assigned to this experiment. To investigate the safety of propolis in beagle dogs, we performed oral administration of propolis (5%) for 8 weeks. Among six beagles, three beagle dogs were randomly allocated to the control group which were fed only regular fodder, and the other three dogs were assigned as the treatment group which were fed regular fodder and propolis (5%). No clinical signs were observed in neither group throughout the experimental period. During the experimental period, there were no significant change in feed intake, water consumption, and body condition. Also, there were no statistically significant differences in hematological and biochemical analyses between the control group and the treated group. Our safety study showed that oral consumption of propolis did not cause any toxicological effects in beagle dogs.

• 한국동물위생학회지
• /
• 제33권2호
• /
• pp.199-206
• /
• 2010

To evaluate the hypoglycemic effect of nano powder propolis, streptozotocin (STZ) induced diabetic rats were divided into 2 groups : diabetic control group and nano powder propolis (0.9ml) group. Then the rats were fed with nano-powder-propolis for 4 weeks. After 4 weeks, oral glucose tolerance test (oral GTT) was performed and blood sugar levels after 16 hours fasting, body weights, and blood lipid levels were measured. Finally, pancreas were collected and examined by histopathology and immunohistochemistry. In conclusion, the nano-powder-propolis was effective in the treatment of diabetes due to the reduction of blood sugar level and the regeneration of damaged ${\beta}$-cells observed in streptozotocin-induced diabetic rats.

• Nutrition Research and Practice
• /
• 제12권6호
• /
• pp.535-540
• /
• 2018

BACKGROUND/OBJECTIVES: Propolis has a rich source of bioactive compounds and has renal and hepatic protective properties. The purpose of this study was to investigate the beneficial effect of hydro-ethanolic extract of propolis against paracetamol-induced liver damage and impairment of kidney function, as well as hematological changes in rats. MATERIALS/METHODS: Six groups of rats were used; the first group was served as a control; the second and third groups were treated by propolis extract at a dose of 50 and 100 mg/kg.B.WT. respectively; the fourth group was treated by paracetamol (200 mg/kg.B.WT.); the fifth group was treated by propolis (50 mg/kg.B.WT.) for eight days and then received similar dose of propolis for following seven days with paracetamol at a dose of 200 mg/kg.B.WT. daily for the seven days; and the sixth group was treated with propolis (100 mg/kg.B.WT.) for eight days and then received similar dose of propolis for following seven days with paracetamol at a dose of 200 mg/kg.B.WT. daily for the seven days. All the animals were treated for a period of 15 days. At the end of the experimental period, blood samples were collected for measurement of the liver enzymes, serum albumin, protein and creatinine, blood urea nitrogen, hematological parameters, and urine volume, protein and albumin. RESULTS: Paracetamol over dose significantly lowered hemoglobin, serum total protein, albumin, and uric acid, while it significantly increased blood creatinine, blood urea nitrogen, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase activities, white blood cells, and platelet count as compared to the control. However, these alterations were significantly attenuated by the use of propolis extract and the effect was dose dependent. Interestingly, propolis prevented paracetamol induced proteinuria, low hemoglobin and body weight loss. CONCLUSIONS: Propolis significantly prevented paracetamol induced renal, hepatic and hematological toxicity and might be useful in the management of liver and renal diseases particularly proteinuria.

• 대한의생명과학회지
• /
• 제12권1호
• /
• pp.43-48
• /
• 2006

Present study aimed to investigate recovering effect of propolis on blood components and tissues of mouse after low dose irradiation. It is verified that the contents of Fe, Mg, P, Zn and Cu in propolis dosed blood are increased slightly than irradiated blood, however, the contents of Ba and Pb are decreased to one tenth than irradiated blood and the contents of Fe and P are increased to 10% than control group. We consider this result as the propolis acts a role of defence factor minimizing changes of elements caused by irradiation in blood. Among the blood components, Glutamate oxaloacetate transaminase (GOT) value is increased after the radiation but after dosed with propolis and irradiated the value is decreased, suggesting that propolis as a buffering material against irradiation. After dosed with propolis, a number of spermatogenic cells are lowered in testis tissue, however, nucleus and cytoplasm are clearly observed in spermatogonia, spermatocytes and spermatid cells. And nucleus and membrane of cells in the proximal convoluted tubule of renal tissue are clearly observed. Also, cytoplasmand membrane of surface mucous cells in stomach tissue are appeared in normal which is almost like those of control group. We consider that the propolis used in this study is preventing deformations of cells increasing resistance capacity against irradiation rather than recovering damaged tissues.

• 한국환경보건학회지
• /
• 제31권1호
• /
• pp.47-54
• /
• 2005

To evaluate the hypoglycemic effect of propolis, streptozotocin (STZ) induced diabetic rats were divided into 4 groups such as, diabetic control group, low dose of propolis (0.1 ml) group, medium dose of propolis (0.3 ml) group and high dose of propolis (0.9 ml) group and feeded with propolis extracts for 30 days. After experiment, oral glucose tolerance test (Oral GTT) was carried, and 16 hours fasting blood sugar levels, body weights, blood lipid levels were measured. Finally, pancreatic histopathological study was performed. In conclusion, the propolis is effective to the treatment diabetes due to the reduction of the blood sugar level and the regeneration of the damaged $\beta$-cells shown in streptozotocininduced diabetic rats.

• 대한의생명과학회지
• /
• 제15권3호
• /
• pp.241-247
• /
• 2009

Wound healing is a complex and highly organized biological response to injury that results in the loss of tissue integrity. Our particular interest was propolis, traditional used as an antimicrobial and an anti-inflammatory agent. The purpose of this study was to see the effects of propolis on healing of the laser induced wounds and the level of collagen formation. 808 nm laser (20 J) was irradiated on the back of rats. Irradiated wounds were divided into gel control and 6 experimental groups (3 and 5% of different three kinds of propolis). Gel type of propolis (supplied by Seoul Propolis Co., Daejeon, Korea) in water based were applied on the laser wound once daily for 14 days. One fourth of rats were sacrificed on $3^{rd}$, $7^{th}$, $10^{th}$, and $14^{th}$ day and each wound was evaluated for degree of wound healing and the level of collagen formation. Healing of wound was evaluated by measuring and comparing the width and depth of the wounds. The levels of collagen were increased in 3 and 5% gel groups comparing to control on $3^{rd}$ day and the collagen level were increased more on $7^{th}$ day. Wounds of the experimental groups showed better healing in width comparing to the healing of the control. The results of this study demonstrated that propolis accelerated healing of wounds induced by laser irradiation and good collagen formation.