• Journal of Environmental Science International
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• v.12 no.6
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• pp.573-582
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• 2003

According to the survey map, the altitudes of up and down the Ansungchon were 242m and 49m accordingly. The width of the river was 4m at upperstream and became wider, lam at midstream and 114m at downstream. Water pH of the Ansungchon was measured variously in the range of 6.0-7.9 according to the surveyed times and areas. The water pH measured during June through August decreased continually and suddenly increased at the end of October. Phragmites iaponica, a major species of the fresh water emergent anchored hydrophytes, was surveyed mostly in the area of upperstream, Phragmites communis, Miscanthus sacchariflorus and Typha orientalis were surveyed in the areas of midstream and downstream, and Zizania latifolia was found in all areas of the river. The total of 101 taxa dividing into 32 families, 71 genera, 88 species, and 13 varieties have been inhabited in these areas. Among the inhabited plants, Poaceae was 21.8%, which appeared in the highest percent. Compositae was 20.8%, and Polygonaceae was 11.9%. In the surveyed areas, herbaceous plants were 95.1% and mostly occupied, and woody plants were 4.9％. Among the herbaceous plants, perennial plants were 52.5%, and annual and biannual plants were 28.7% and 13.9% accordingly. Naturalized plants dividing into 8 families and 18 genera have been found in the surveyed areas. The number of the naturalized plants increased from upperstream to downstream as the environmental disruption became worse. Only 2.5% of the total naturalized plants were appeared in up, 17.7% in midstream, and 28.6% in downstream. Shannon-Weiner's index for the degree of diversity was 2.898-3.666 in the area of upperstream, but 3.708-4.135 in downstream, which was little higher.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.3
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• pp.14-25
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• 2004

The purpose of this study was investigated to the change of vegetation coverage by elapsed years on the cut slope of forest road in Jinju-si. The results obtained could be summarized as follows; The plant coverage on the cut slope of forest road was decreased hastily in first and second year after seeding. The plant coverage on the cut slope of forest road was increased as growth of seed-sprayed from third year. But the plants were began to competition with between sprayed seeds and invaded plants from third to fifth year after seed-spray. The numbers of invading plants were gradually increased as 581 individuals from sixth year after seed-spray. The cut slopes of the forest roads turned to a good site condition for growing of invasion plants. And also the total coverage on cut slope of forest roads by invading of surrounding plants was increased more. It showed that plant invasion on cut-slopes of forest roads would be mostly influenced by surrounding plants. The number of surrounding plants on the cut-slopes of forest roads was 59 species, and the number of invading plants showed 65 species. The invading species were high in order of Boehmeria tricuspis, Oplismens undulatifolius, Miscanthus sinensis var. purpurascens, Erechtites hieracifolia and Artemisia princeps var. Orientalis In the stepwise regression analysis, main factors affecting the coverage of vegetation on the cut-slopes of forest road showed in order of elapsed years, gravel contents(30~50%), middle, sandy loam, sandy clay-loam, soil hardness, aspect(NS), concave(凹) type and gravel contents(15~30%).

• The Korea Journal of Herbology
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• v.25 no.3
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• pp.139-147
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• 2010

Objectives：For the purpose of developing Korean herbalogy of the plants belonging to Crassulaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article. Methods：The examined herbalogical books and research papers which published at home and abroad. Results： 1. There were totaled to 6 genera and 39 species in Crassulaceae in Korea and among them medicinal plants are 5 genera, 14 species, some 36% in total. 2. Sedum genus is main kind enough that it has 20 species among 39 species in the Crassulaceae, of which medicinal plants are 9 species. 3. The herb is the main medicinal parts of medicinal plants in the Crassulaceae which is used in 14 species. 4. According to nature and flavor of medicinal plants in the Crassulaceae, they were classified into neutral 12 species; sour taste 14 in the order. 5. According to meridian propism of medicinal plants in the Crassulaceae, they were classified into liver meridian 13 species. 6. According to the properties and principal curative action in the Crassulaceae, they were classified into drugs for detoxicant 19 species, drugs for styptic 18, drugs for detumescence 14 in the order. 7. The number of toxic species in the Crassulaceae was examined to be 3 species. Conclusions：There were totaled to 6 genera and 39 species in Crassulaceae in Korea and among them medicinal plants are 5 genera, 14 species, some 36% in total.

• The Plant Pathology Journal
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• v.36 no.5
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• pp.450-458
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• 2020

Pepper and tomato plants infected with two Clavibacter species, C. capsici and C. michiganensis have shown different patterns of disease development depending on their virulence. Here, we investigated how pepper and tomato plants respond to infection by the high-virulent or low-virulent Clavibacter strains. For this, we chose two strains of each Clavibacter species to show different virulence level in the host plants. Although low-virulent strains showed less disease symptoms, they grew almost the same level as the high-virulent strains in both plants. To further examine the response of host plants to Clavibacter infection, we analyzed the expression patterns of plant defense-related genes in the leaves inoculated with different strains of C. capsici and C. michiganensis. Pepper plants infected with high-virulent C. capsici strain highly induced the expression of CaPR1, CaDEF, CaPR4b, CaPR10, and CaLOX1 at 5 days after inoculation (dai), but their expression was much less in low-virulent Clavibacter infection. Expression of CaSAR8.2 was induced at 2 dai, regardless of virulence level. Expression of GluA, Pin2, and PR2 in tomato plants infected with high-virulent C. michiganensis were much higher at 5 dai, compared with mock or low-virulent strain. Expression of PR1a, Osmotin-like, Chitinase, and Chitinase class 2 was increased, regardless of virulence level. Expression of LoxA gene was not affected by Clavibacter inoculation. These results suggested that Clavibacter infection promotes induction of certain defense-related genes in host plants and that differential expression of those genes by low-virulent Clavibacter infection might be affected by their endophytic lifestyle in plants.

• Journal of Microbiology and Biotechnology
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• v.9 no.2
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• pp.213-218
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• 1999

Fructans are polyfructose molecules that function as nonstructural storage carbohydrates in several plants. In addition, it has been suggested that, due to their solubility, they can play an important role in helping plants survive periods of osmotic stress. In order to study the effect of levan synthesis on plant growth, the coding region of the levansucrase gene, which was isolated from Zymomonas mobilis, was introduced into tobacco plants using Agrobacterium tumefaciens-mediated transformation. The presence of the levansucrase gene in transgenic plants was verified by genomic DNA gel blot analysis. RNA gel blot and immunoblot analyses showed an accumulation of the corresponding transcript and protein product of the bacterial levansucrase gene in transgenic plants. Furthermore, a thin layer chromatography analysis revealed that fructans were synthesized and deposited in transgenic tobacco plants. When $T_1$ seeds were germinated and grown under polyethylene glycol-mediated drought stress or cold stress, the transgenic seedlings displayed a substantially higher level of growth than that of untransformed plants. These results suggest that fructans may playa significant role in the tolerance of plants under osmotic stress.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.71-77
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• 2011

Drought and salinity are the most important abiotic stresses that affect the normal growth and development of plants. Glycine betaine is one of the most important osmolytes present in higher plants that enable them to cope with environmental stresses through osmotic adjustment. In this study, a betaine aldehyde dehydrogenase (BADH) gene from spinach under the control of the stress-induced promoter rd29A from Arabidopsis thaliana was introduced into potato cultivar Gannongshu 2 by the Agrobacterium tumefaciens system. Putative transgenic plants were confirmed by Southern blot analysis. Northern hybridization analysis demonstrated that expression of BADH gene was induced by drought and NaCl stress in the transgenic potato plants. The BADH activity in the transgenic potato plants was between 10.8 and 11.7 U. There was a negative relationship (y = -2.2083x + 43.329, r = 0.9495) between BADH activity and the relative electrical conductivity of the transgenic potato plant leaves. Plant height increased by 0.4-0.9 cm and fresh weight per plant increased by 17-29% for the transgenic potato plants under NaCl and polyethylene glycol stresses compared with the control potato plants. These results indicated that the ability of transgenic plants to tolerate drought and salt was increased when their BADH activity was increased.

• BMB Reports
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• v.34 no.4
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• pp.316-321
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• 2001

Dehydroascorbate (DHA) reductase (DHAR, EC 1.8.5.1) catalyzes the reduction of DHA to reduced ascorbate (AsA) using glutathione (GSH) as the electron donor in order to maintain an appropriate level of ascorbate in plant cells. To analyze the physiological role of DHAR in environmental stress adaptation, we developed transgenic tobacco (Nicotiana tabacum cv. Xanthi) plants that express a human DHAR gene isolated from the human fetal liver cDNA library in the chloroplasts. We also investigated the DHAR activity, levels of ascorbate, and GSH. Two transgenic plants were successfully developed by Agrobacterium-mediated transformation and were confirmed by PCR and Southern blot analysis. DHAR activity and AsA content in mature leaves of transgenic plants were approximately 1.41 and 1.95 times higher than in the non-transgenic (NT) plants, respectively In addition, the content of oxidized glutathione (GSSG) in transgenic plants was approximately 2.95 times higher than in the NT plants. The ratios of AsA to DHA and GSSG to GSH were changed by overexpression of DHAR, as expected, even though the total content of ascorbate and glutathione was not significantly changed. When tobacco leaf discs were subjected to methyl viologen at $5\;{\mu}M$, $T_0$ transgenic plants showed about a 50% reduction in membrane damage compared to the NT plants.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.6
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• pp.63-75
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• 2013

The purpose of the study is investigating the price fluctuation of landscaping plants in the Information on Commodity Prices(ICP) and the posted price fluctuation of landscaping plants of Public Procurement Service(PPS) recent 10 years. It also provides the basic information which can be applied to production and sales of landscaping plants, comparing with general price index. The major findings of the study are as follows. First, The price of investigated plants of PPS has increased about 4.56% in average recent 10 years. Among this increase, of evergreen tree was predominant. On the other hand, landscaping trees price of ICP has increased about only 2.34% in average. Secondly, The result shows that average price of investigated plants of PPS is positively related with the price of ICP. For this reason, we found that prices of ICP and of PPS move together in most case. However, we found that there are no relation between Consumer Price Index(CPI), Producer Price Index(PPI) and Agricultural Price Index(API). Therefore, price fluctuation of landscaping trees moves regardless of normal price fluctuation in general. Third, even though result shows that price index of evergreen trees, deciduous trees and shrubs are weakly related with normal price index partly, it was not high enough to be significant. According to the result, we found that price of landscaping plants is not related with market situation. For this reason, we thought that there are some difficulties for the reasonable production and sales of landscaping plants because the price is somewhat decided by rule of thumb. Therefore, understanding the composition of cost and making prediction by price fluctuation available are needed so that it can be practically conducive to reasonable production and sales.

• The Plant Pathology Journal
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• v.19 no.2
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• pp.85-91
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• 2003

Pre-treatment with DL-3-aminobutyric acid (BABA) in the cucumber plants caused the decrease of disease severity after inoculation with anthracnose pathogen Colletotrichum orbiculare. In this study, ultrastructures of the vascular bundle and the infection structures in the leaves of BABA-treated as well as untreated cucumber plants were observed after inoculation with the anthracnose pathogen by electron microscopy. The ultrastructures of vascular bundle in the leaves of BABA-treated plants were similar to those of the untreated plants except plasmodesmata. In the BABA-treated plants, the plasmodesmata were more numerous than in the untreated plants, suggesting that the BABA treatment may cause the active transfer of metabolites through the vascular bundle. In the leaves of untreated plants, the fungal hyphae were spread widely in the plant tissues at 5 days after pathogen inoculation. Most cellular organelles in the hyphae were intact, indicating a compatible interaction between the plant and the parasite. In contrast, in the leaves of BABA pre-treated plants the growth of most hyphae was restricted to the epidermal cell layer at 5 days after inoculation. Most hyphae cytoplasm and nucleoplasm was electron dense or the intracellular organelles were degenerated. The cell walls of some plant cells became thick at the site adjacent to the intercellular hyphae, indicating a mechanical defense reaction of the plant cells against the fungal attack. Furthermore, hypersensitive reaction (HR) of the epidermal cells was often observed, in which the intracellular hyphae were degenerated. Based on these results it is suggested that BABA causes the enhancement of defense mechanisms in the cucumber plants such as cell wall apposition or HR against the invasion of C. orbiculare.

• The Plant Pathology Journal
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• v.29 no.2
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• pp.193-200
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• 2013

Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 weeks post inoculation (WPI). CMV titre in Arabidopsis leaves was determined by indirect enzyme-linked immunosorbent assay (ELISA) at 2 WPI. Our results demonstrated that among all Arabidopsis plants treated with barley grain inoculum (BGI) of SKT-1 NahG and npr1 plants showed no significant reduction in disease severity and CMV titre as compared with control plants. In contrast, disease severity and CMV titre were significantly reduced in all Arabidopsis plants treated with culture filtrate (CF) of SKT-1 as compared with control plants. RT-PCR results showed increased expression levels of SA-inducible genes, but not JA/ET-inducible genes, in leaves of BGI treated plants. Moreover, expression levels of SA- and JA/ET-inducible genes were increased in leaves of CF treated plants. In conclusion, BGI treatment induced systemic resistance against CMV through SA signaling cascade in Arabidopsis plants. While, treatment with CF of SKT-1 mediated the expression of a majority of the various pathogen related genes, which led to the increased defense mechanism against CMV infection.