• Journal of Acupuncture Research
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• v.30 no.5
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• pp.25-39
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• 2013

Objectives : This study was designed to find out the differences of the acupuncture sensation by type of pharmacopuncture. And furthermore we try to find out whether normal saline(NS) is able to be constituted as an appropriate control group for the Calculus Bovis Fel Ursi Moschus(BUM) pharmacopuncture, mountain ginseng pharmacopuncture and sciatica no. 5 pharmacopuncture. Methods : NS and three type of pharmacopunctures were inserted into $ST_{36}$, and $ST_{37}$ of the subjects. Before and after the treatment, subjects completed a questionnaire rating the intensity of 13 kinds of acupuncture sensation(acupuncture sensation scale, ASS). We compared the subjective acupuncture sensation between the NS and three type of pharmacopunctures. Results : BUM pharmacopuncture showed significantly intense acupuncture sensation comparing other two pharmacopunctures and NS. There was no statistically significant difference among mountain ginseng pharmacopuncture, sciatica no. 5 pharmacopuncture and NS. Conclusions : We found that NS may be able to be an placebo pharmacopuncture for mountain ginseng pharmacopuncture and sciatica no. 5 pharmacopuncture. Additional study is needed for placebo pharmacopuncture of BUM pharmacopuncture.

• Mycobiology
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• v.42 no.2
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• pp.147-151
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• 2014

We investigated the diversity of the foliar endophytes of Korean ginseng. Endophytic fungi were isolated from healthy leaves of mountain-cultivated ginseng (MCG) and field-cultivated ginseng (FCG) at 4 sites in Chungbuk Province. A total of 24 species of fungal endophytes were identified using molecular approaches. Additionally, the diversity of these endophytic fungi was compared between MCG and FCG. The major isolated endophytes were Edenia gomezpompae and Gibberella moniliformis in the MCG and FCG samples, respectively. The results suggest that ginseng endophytes have different community structures in different environments, and this understanding may prove useful in ginseng cultivation.

• Laboraroty Animal Research
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• v.33 no.2
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• pp.105-113
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• 2017

insenosides from Panax ginseng are well known for their diverse pharmacological effects including antithrombotic activity. Since adventitious roots of mountain ginseng (ARMG) also contain various ginsenosides, blood flow-improving effects of the dried powder and extract of ARMG were investigated. Rats were orally administered with dried powder (PARMG) or ethanol extract (EARMG) of ARMG (125, 250 or 500 mg/kg) or aspirin (30 mg/kg, a reference control) for 3 weeks. Forty min after the final administration, carotid arterial thrombosis was induced by applying a 70% $FeCl_3$-soaked filter paper outside the arterial wall for 5 min, and the blood flow was monitored with a laser Doppler probe. Both PARMG and EARMG delayed the $FeCl_3$-induced arterial occlusion in a dose-dependent manner, doubling the occlusion time at high doses. In mechanism studies, a high concentration of EARMG inhibited platelet aggregation induced by collagen in vitro. In addition, EARMG improved the blood lipid profiles, decreasing triglyceride and cholesterol levels. Although additional action mechanisms remain to be clarified, it is suggested that ARMG containing high amount of ginsenosides such as $Rg_3$ improves blood flow not only by inhibiting oxidative thrombosis, but also by modifying blood lipid profiles.

• Journal of Ginseng Research
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• v.48 no.1
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• pp.98-102
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• 2024

Background: Ginseng (Panax ginseng Meyer) is a perennial plant belonging to the Araliaceae family that is known to have various beneficial effects including improving memory loss and spatial cognitive ability, and anti-cancer and anti-diabetes activity. Its functional benefits also include improving liver function, regulating blood pressure, stress, and providing antioxidant activity. Usually, various agrochemicals are used in cultivating ginseng preventing from many diseases. Methods: FCGP (field cultivated ginseng in pot) was implemented by imitating MCWG (mountain cultivated wild ginseng). Pesticide analysis of pot cultivation was carried out and the contents of bioactive components such as ginsenoside were also analyzed. Results: FCGP ginsenoside content was higher than that of FCG (field cultivated ginseng) and MCWG. FCGP has been shown to have a relatively high antioxidant effect compared with cultivated ginseng. Conclusion: It was confirmed that ginseng can be grown for 6 years without resorting to use of pesticides. In addition, it was confirmed that effective accumulation of physiologically active ingredients such as ginsenoside is possible. Our result represents FCGP is a novel method of pesticide-free ginseng cultivation

• Journal of Ginseng Research
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• v.43 no.4
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• pp.600-605
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• 2019

Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.

• Journal of Ginseng Culture
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• v.4
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• pp.59-102
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• 2022

Since there were only few countries that could find wild ginseng grown in nature, the culture of digging wild ginseng was only developed in a small number of countries. In a time when the orthodox head-Simmemani has disappeared, the tangible and intangible culture of Simmemani (wild ginseng digger) is disappearing more and more with the passage of time. So far, the conducted research on wild ginseng diggers was very partial and simplistic as follows: ① Research on the argot of Simmemani, ② Research on the customs of Simmemani, ③ Research on the change of customs of Simmemani, ④ Additional records through interview with Simmemani. Accordingly, no comprehensive study on the Simmemani culture has been done yet. This study supplements the historical materials that were not reflected in previous studies, and discuss on diverse subjects including the definition and classification of wild ginseng, the distribution of wild ginseng and Simmemani, interpretation of wild ginseng digging from a legal and social point of view, the organization responsible for the digging, determination of the date of entering the mountain, preparations, taboos, departure and entry into a mountain, religious events, psalmbook, dream interpretation, search and discovery of wild ginseng, digging, profit sharing, the amount of harvested wild ginseng, and the price of wild ginseng. In addition, Korean wild ginseng digging culture was comprehensively studied by attaching the photos and illustrations of historical documents with the psalmbook of the head-Simmemani.

• Journal of Ginseng Research
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• v.30 no.3
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• pp.172-180
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• 2006

The purpose of this study is classifying types of American ginseng and estimating their production cost and revenue by the types. Usually, the American ginseng can be classified as 4 different types; wild ginseng(WG), wild simulated ginseng(WSG), woods grown ginseng(WGG), and field cultivated ginseng(FCG). This paper estimates costs and benefits for FCG, WGG, and WSG per acre. The WGG & WSG are produced under the tree at mountain while the FCG is produced at large scale farm with machinery. Annual profit for the FCG is $2,222 while that of the WGG and the WSG are $2,759 and $3,799 per acre. Although quantity produced per acre for the WGG and WSG(600lbs and 160lbs) are much smaller than that of the FCG(3,000lbs), prices per pound for the WGG and WSG($125, 375$) are higher than that of the FCG($24). In addition, production costs for the WGG and WSG are lower than that of the FCG because of the costs for seeds, shadow facility, and chemicals are different by the types of production.

• Journal of Pharmacopuncture
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• v.17 no.3
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• pp.74-77
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• 2014

Objectives: The purpose of this study is to report a case of a plexiform neurofibroma (PNF) in the pelvic region treated with sweet bee venom (SBV) and mountain ginseng pharmacopuncture (MGP). Methods: A 16-year-old girl was diagnosed as having PNFs, neurofibromatosis type 1, 10 years ago and she had surgery three times to remove the benign tumors, but the growth of the PNFs continued. She has been treated in our clinic with SBV and MGP two times per month from March 2010 to April 2014. SBV was injected intra-subcutaneously at the borders of the PNFs in the pelvic region, and MGP was administrated intravenously each treatment time. Results: The growths of the PNFs occurred rapidly and continued steadily before treatment. Since March 2010, she has been treated in our clinic, and the growths of the PNFs have almost stopped; further-more, the discomfort of hip joint pain has been reduced, and her general condition has improved. Conclusion: We cautiously conclude that SBV and MGP treatment has some effects that suppress the growth and the spread of the PNFs in this patient.

• Journal of Oriental Neuropsychiatry
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• v.21 no.1
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• pp.125-144
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• 2010

Objectives: The purpose of this study was to characterize the effect of the fraction of Korean mountain ginseng folium (FKG) on the learning and memory impairments induced by scopolamine. Methods: The memory ameliorating effect of FKG was investigated using a passive avoidance test, the Y-maze test, and the Morris water maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine(1mg/kg, i.p.). Results: FKG (2 or 4mg/kg, p.o.) administration significantly reversed scopolamine-induced cognitive impairments in mice by the passive avoidance test and the Y-maze test(P<0.05), and also improved escape latency in the Morris water maze test at 2 or 4mg/kg(P<0.05). Although FKG has little inhibitory activity for AChE (IC50 value; 1847 ${\mu}g/ml$) in an invitro study, phosphorylated extracellular signal-regulated kinase(pERK) was increased by the administration of FKG inhippocampus on immunohistochemistry. Conclusions: These results suggest that FKG may be a useful cognitive impairment treatment, and its beneficial effects are mediated, in part, via activation of ERK pathway.

• Journal of Korean Society of Forest Science
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• v.104 no.3
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• pp.383-389
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• 2015

The purpose of this study was to investigate the anti-apoptosis-related protein expression in skeletal muscle of rats with different amount of mountain ginseng (MG) added high-fat diet fed. Twenty-four Sprague Dawley male rats were divided into the high-fat diet control group (CON), 0.5% of MG added diet group (MG1), and 1.0% of MG added diet group (MG2) with eight rats each. The P53, anti-apoptotic protein, was significantly lower in MG2 than CON and MG1. The bcl-2 and bcl-xl, however, were not significantly different from MG1 but from CON. The caspase-9 and -3, were significantly lower in MG groups than CON. In addition, it was dramatically lower in MG2. These results suggested that MG addition to the high-fat diet suppressed p53 protein expression and enhanced anti-apoptototic protein expression. MG may be a positive effects on health as a medicinal plant.