Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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The Study of Mountain Ginseng-added High Fat Diet on Anti-Apoptosis of Skeletal Muscle

산양삼 첨가 고지방식이가 골격근의 항세포사멸에 대한 작용 연구

  • Seo, Hyobin (Department of Leisure Sports, Kyungpook National University) ;
  • Kim, Pan-Gi (School of Ecological & Environmental Systems, Kyungpook National University) ;
  • Ryu, Sungpil (Department of Leisure Sports, Kyungpook National University)
  • 서효빈 (경북대학교 레저스포츠학과) ;
  • 김판기 (경북대학교 생태환경시스템학부) ;
  • 류승필 (경북대학교 레저스포츠학과)
  • Received : 2015.01.20
  • Accepted : 2015.09.10
  • Published : 2015.09.30
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Abstract

The purpose of this study was to investigate the anti-apoptosis-related protein expression in skeletal muscle of rats with different amount of mountain ginseng (MG) added high-fat diet fed. Twenty-four Sprague Dawley male rats were divided into the high-fat diet control group (CON), 0.5% of MG added diet group (MG1), and 1.0% of MG added diet group (MG2) with eight rats each. The P53, anti-apoptotic protein, was significantly lower in MG2 than CON and MG1. The bcl-2 and bcl-xl, however, were not significantly different from MG1 but from CON. The caspase-9 and -3, were significantly lower in MG groups than CON. In addition, it was dramatically lower in MG2. These results suggested that MG addition to the high-fat diet suppressed p53 protein expression and enhanced anti-apoptototic protein expression. MG may be a positive effects on health as a medicinal plant.

본 연구에서는 고지방식이에 산양삼 첨가량을 달리하였을 때, 흰쥐의 골격근에서 항세포사멸 단백질 발현을 보고자 하였다. 24마리의 SD계 수컷 흰쥐를 대상으로 CON(고지방식이군), MG1(0.5% 산양삼 첨가 식이군), MG2(1.0% 산양삼 첨가 식이군)으로 각 그룹별 8마리씩 분류하였다. 4주간의 각 식이섭취의 결과 세포사멸 활성유전자인 p53은 MG2군이 CON군과 MG1군에 비하여 통계적으로 유의하게 낮게 나타났으며, 항세포사멸 인자인 bcl-2와 bcl-xl은 MG1군과 유의한 차이는 나지 않았으나 CON군보다 높은 발현량을 나타내었다. 최종 세포사멸의 인자인 caspase-9 및 3는 CON군과 비교하여 산양삼을 섭취한 그룹에서 낮은 발현량을 나타내었다. 특히, MG2군에서는 유의하게 낮은 발현량을 나타내어 고지방식이에 산양삼 첨가는 골격근에서 세포사멸 활성 유전자인 p53을 억제하고, 항세포사멸 유전자의 활성을 증가시켜 최종 세포사멸인자의 활성을 저해하여 약용식물로서의 인체에 긍정적인 역할을 할 것이라 사료된다.

Keywords

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