• BMB Reports
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• 제35권6호
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• pp.629-636
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• 2002

Protein kinase CKII (CKII) is required for progression through the cell division cycle. We recently reported that the $\beta$ subunit of protein kinase CKII ($CKII{\beta}$) associates with CKBBP1 that contains the Ring-H2 finger motif in the yeast two-hybrid system. We demonstrate here that the Ring-H2 finger-disrupted mutant of CKBBP1 does not interact with purified $CKII{\beta}$ in vitro, which shows that the Ring-H2 finger motif is critical for direct interaction with $CKII{\beta}$. The CKII holoenzyme is efficiently co-precipitated with the wild-type CKBBP1, but not with the Ring-H2 finger-disrupted CKBBP1, from whole cell extracts when epitope-tagged CKBBP1 is transiently expressed in HeLa cells. Disruption of the Ring-H2 finger motif does not affect the cellular localization of CKBBP1 in HeLa cells. The increased expression of either the wild-type CKBBP1 or Ring-H2 finger-disrupted CKBBP1 does not modulate the protein or the activity levels of CKII in HeLa cells. However, the stable expression of Ring-H2 finger-disrupted CKBBP1 in HeLa cells suppresses cell proliferation and causes the accumulation of the G1/G0 peak of the cell cycle. The Ring-H2 finger motif is required for maximal CKBBP1 phosphorylation by CKII, suggesting that the stable binding of CKBBP1 to CKII is necessary for its efficient phosphorylation. Taken together, these results suggest that the complex formation of $CKII{\beta}$ with CKBBP1 and/or CKII-mediated CKBBP1 phosphorylation is important for the G1/S phase transition of the cell cycle.

• Bulletin of the Korean Chemical Society
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• 제34권11호
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• pp.3471-3473
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• 2013

• 복식문화연구
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• 제23권3호
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• pp.368-383
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• 2015

The purpose of this study is to find an expression method for Korean fashion design that can have competitiveness in the global fashion market. For this, fashion specialists at home and abroad were interviewed, focusing on their opinions on the Korean motifs outlined in existing literature studies. First, aesthetic sense and expression characteristics that have appeared in existing studies on Korean art, Korean traditional costume, and Korean fashion were explored. As a result, they were categorized into: Modesty/plainness, freewheelingness, religion/custom, and humor/joke. During this process, Korean design motifs were extracted based on the aesthetic characteristic and expression method of aesthetic sense. According to the opinions on Korean design motifs and Korean fashion design works drawn from the interviews with fashion specialists at home and abroad, Changhomun was the favorite design motif to be utilized in Korean fashion design. The Korean specialists thought that visual design motifs were important, while the overseas specialists had more interest in abstract design motifs, which embody the background and story behind the motif. Regarding the fashion design associated with the Korean design motif, all specialists at home and abroad favored the design using ceramics and wrapping cloth. Regarding the choice of fashion image, Korean specialists chose the design utilizing Hangeul as the most Korean, while the foreign specialists chose designs reflecting the design motifs of Hangeul and Hanbok for women. The Korean specialists chose relatively more Korean images, while the foreign specialists chose more eastern images or other images than Korean images.

• 한국의상디자인학회지
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• 제10권2호
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• pp.69-78
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• 2008

The purpose of this study is to develop a plan that adopts a fairly tale motif, which is familiar to children, to traditional Korean costume design factors for the development of practical Korean costume enterprises, which lead the Korean costume industry in its existence. Literary research and previous research methods had been used in this study. After comparing traditional Korean costume factors with practical Korean costume factors through literary research, we examined children's Korean costumes and ordinary children costumes. The results of this study are the following: First, the result of literary research, which used traditional Korean costume as motif of practical Korean costume and adopted model factors that the developed motif had used, shows that the design factors of practical Korean Costumes have changed from traditional to modern, seeking practicability and convenience over appearance. Second, practical Korean costumes are suitable when fairy tale illustration is applied to children with curiosity and warm emotions, and towards children, who spend much time on activity during growth. Third, making use of CAD system, which is different from practical Korean costume based on traditional patterns and single color, and using various pint pattern with many stories for the design of practical Korean costumes, and applying diversity to the market of practical Korean costumes, this study provides new design motif from, which people can feel more familiar to practical Korean costumes.

• 정보관리학회지
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• 제23권4호
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• pp.69-87
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• 2006

소설을 비롯한 문학작품에 대한 접근은 기술요소 중심이었고, 주제접근 역시 작품 속에 등장하는 소재, 인물명, 지명 등 형식 요소에 국한되어 왔다. 이러한 관행은 소설 주제의 본질을 놓친 것이며 미학적 경험을 추구하는 이용자의 주제요구를 반영하지 못한다 이 연구에서는 소설 주제접근체계의 확장을 위해 상징 및 모티프의 개념과 주제접근 점으로서의 가능성을 검토하였다. 이와 함께 해당 용어사전을 정보원으로 활용하여 상징과 모티프 체계를 구성하고, 20세기 한국소설에 적용해 이용성과 한계점을 논하였다.

• 한국자기공명학회논문지
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• 제11권1호
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• pp.24-29
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• 2007

[ ${\beta}ig-h3$ ] is an extracellular matrix protein that mediates cell adhesion through interaction with integrins. The 18 residue YH motifs within each fas-1 domain are known to be responsible for the interaction with the ${\alpha}_v{\beta}_5$ integrin, and the synthetic YH motif peptides are known to inhibit endothelial tube formation and reduces the number of blood vessels, and so expected to be an effective inhibitor of angiogenesis. In this study, we solved the 3D structure of the 18 residue YH motif peptide (EALRDLLNNHILKSAMCA; D2 peptide) within the second fas-1 domain of ${\beta}ig-h3$ using NMR. The Peptide has ${\alpha}-helix$ structure at the C terminal region but the N terminal region is flexible. The present structural information may be helpful for developing more effective peptide drug candidate for the treatment of diseases dependent on angiogenesis.

• Journal of Life Science
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• 제10권1호
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• pp.37-39
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• 2000

Testis-specific protein Y(TSPY) is thought to play an important role during spermatogenesis in primates. The Arg and Lys-rich region of TSPY was implicated as a potential DNA binding site in the human and Japanese monkey. In the study, we investigated this further through looking at the putative amino acid sequences of Arg and Lys-rich region of TSPY gene from seven species of gibbons, five species of Old World monkeys and five species of New World monkeys. A comparison with those of the human and Japanese monkey revealed that the Arg and Lys-rich motif was hightly conserved in various primates. This finding suggests that a possible role for the Arg and Lys-rich motif in primate TSPY is DNA recognition. The functional implications for TSPY are discussed in the light of this and previous findings.

• BMB Reports
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• 제43권12호
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• pp.807-812
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• 2010

$NF{\kappa}B$ and ZAS3 are transcription factors that control important cellular processes including immunity, cell survival and apoptosis. Although both proteins bind the ${\kappa}B$-motif, they produce opposite physiological consequences; $NF{\kappa}B$ activates transcription, promotes cell growth and is often found to be constitutively expressed in cancer cells, while ZAS3 generally represses transcription, inhibits cell proliferation and is downregulated in some cancers. Here, we show that ZAS3 inhibits $NF{\kappa}B$-dependent transcription by competing with $NF{\kappa}B$ for the ${\kappa}B$-motif. Transient transfection studies show that N-terminal 645 amino acids is sufficient to repress transcription activated by $NF{\kappa}B$, and that the identical region also possesses intrinsic repression activity to inhibit basal transcription from a promoter. Finally, in vitro DNA-protein interaction analysis shows that ZAS3 is able to displace $NF{\kappa}B$ by competing with $NF{\kappa}B$ for the ${\kappa}B$-motif. It is conceivable that ZAS3 has therapeutic potential for controlling aberrant activation of $NF{\kappa}B$ in various diseases.

• 복식
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• 제30권
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• pp.307-320
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• 1996

The purpose of this study is to classify of the ancient belt 'the Gwathai' especially in the timesof three countries. For this purpose the Gwathai was classified as to the motif which was decorated on the plate of the Gwathai and also another parts of the Gwathai were studied. The Gwathai could be classified of three parts. The first rectangular plate and the hanging part with heart motif and the second reverse-heart plate. The character of the third part was the specificity of the plate motif. Also there was a hanging part at the Gwathai and it was organized with the diverse form At first it was used for the practical pur-pose and then later for the decorational pur-pose. But this study could'nt reval the chang channel of the Gwathai and the relation be-tween region. This is the limit of this study. So more study is necessary to clarify this point.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2002년도 가을 학술발표논문집 Vol.29 No.2 (2)
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• pp.235-237
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• 2002

motif란 기능적으로 유사한 단백질 군의 아마노산 서열들에 공통적으로 나타나는 일정한 패턴이나 부분서열을 말한다. 본 논문에서는 motif들로 각 단백질의 특성을 표현한 다음, 이것을 기초로 유사성을 비교하여 단백질들을 기능적으로 유사한 여러개의 계층적 군으로 나누는 군집화 방법을 소개하였다. 영역 특성상 확장성과 계층성을 가지는 신경망 GHSOM을 군집화 알고리즘으로 사용하였고, 실제 307 개의 단백질들에 대한 군집화 실험을 통해 그 효과를 확인해보았다.