• 한국환경복원기술학회지
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• 제13권5호
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• pp.51-58
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• 2010

This study was done to recommend a method for conserving the habitat of Amblyotropis verna grown naturally in campus of the Kyungpook National University at Daegu city. Vegetation characteristics and morphological characteristics of each individual were analyzed for the condition of the population at the growing season from May to June in 2010. Totally 11 vegetation data were collected and analyzed, and one vegetation community including Amblyotropis verna (Amblyotropis verna-Zoysia japonica community) was found. We found that the growing condition was not good with severe stamping and disturbance. The location-determining method by PCoA analysis showed the physical texture in soil was a critical factor determining the spatial distribution of the Amblyotropis verna-Zoysia japonica community. And the analysis of morphological characteristics found that light condition and soil hardness were the main ecological factor determining the size and form of each individual. Finally, this study recommend that minimizing impacts by human stamping and artificial disturbance and reducing the water stress were the best ways for the conservation of Amblyotropis verna habitats.

• ALGAE
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• 제21권2호
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• pp.169-174
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• 2006

We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.

• Restorative Dentistry and Endodontics
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• 제47권3호
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• pp.28.1-28.12
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• 2022

Objectives: Metalloproteinase-inhibiting agents, such as chitosan, can prevent collagen degradation in demineralized dental substrates, thereby improving the adhesive interface. This study evaluated the bond strength (BS) and chemical and morphological characterization of the adhesive interface after applying chitosan solution to demineralized dentin. Materials and Methods: The 80 third molars were selected. Forty teeth underwent caries induction using the pH cycling method. The teeth were divided according to the treatment: distilled water (control) and 2.5% chitosan solution. The surfaces were restored using adhesive and composite resins. Half of the specimens in each group were aged, and the other half underwent immediate analyses. The teeth were sectioned and underwent the microtensile bond strength test (µTBS), and chemical and morphological analyses using energy-dispersive spectroscopy and scanning electron microscopy, respectively. Data analysis was performed using 3-way analysis of variance. Results: For µTBS, sound dentin was superior to demineralized dentin (p < 0.001), chitosan-treated specimens had higher bond strength than the untreated ones (p < 0.001), and those that underwent immediate analysis had higher values than the aged specimens (p = 0.019). No significant differences were observed in the chemical or morphological compositions. Conclusions: Chitosan treatment improved bond strength both immediately and after aging, even in demineralized dentin.

• 한국약용작물학회지
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• 제24권1호
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• pp.55-61
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• 2016

Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers. Methods and Results :We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups. Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.

• 한국균학회지
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• 제47권2호
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• pp.95-104
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• 2019

The genus Ochrolechia is a widespread, lichen genus in Korea. Despite being common, little is known about the species diversity and geographical distribution of Ochrolechia. In this study, we detailed the identification procedure of the genus Ochrolechia in a Korean collection and provided the description of each species. Using 104 specimens collected from 2003 to 2017, we identified four species of the genus Ochrolechia via morphological and/or molecular phylogenetic analysis: O. parellula, O. trochophora, O. yasudae and O. akagiensis. Among them, O. akagiensis had not been previously reported in Korea. Moreover, the species identified as O. frigida and O. tartarea in past studies were corrected as O. yasudae and O. parellula, respectively, based on morphological and/or molecular evidence. Phylogenetic analysis using the internal transcribed spacer regions including 5.8S rRNA gene showed that the four species separated clearly, indicating that the morphological identification corresponds to the phylogenetic identification. We provide a taxonomic key for the four species of the genus Ochrolechia.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 2000년도 동계 학술대회 논문집
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• pp.101-106
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• 2000

• 대한의생명과학회지
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• 제16권3호
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• pp.187-192
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• 2010

The aim of this study was to compare the results of morphological phenotypic examination and molecular biological method, randomly amplified polymorphic DNA (RAPD) using clinical isolates from Korean patients infected with dermatophytes. A total of 44 clinical isolates have been collected by Korean Collection Medical Fungi (KCMF) and those were subjected for the RAPD analysis. The results showed that the fungal strains that have RG1 band patterns were identified as Trichophyton rubrum by the neighbour-joining analysis. Additionally, other fungal strains that have RG3 band patterns were identified as Epidermophyton floccosum and RG5 band patterns were identified as Micorsporum gypseum. Even though molecular biological method such as RAPD are not necessary, it might be useful when some strains have similar morphological characteristics or when same species has phenotypic variations. In this study, therefore, we targeted internal transcribed spacer (ITS) regions of fungal rDNA, performed RAPD and then compared with morphological phenotypic examination.

• Animal cells and systems
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• 제2권4호
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• pp.451-454
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• 1998

Gulgastrura reticulosa, first described by Yosii (1966) as a monotypic new genus from a Korean limestone cave, was reviewed for its systematic position by c1adistic analysis of morphological characteristics, investigation of its intermaulting period and reproductive cycle as well as allozyme and 18S rDNA analysis. The great extent of divergence was strongly suggested by its combined lack of sensory organs (third antennal organ, postantennal organ, eyes, pseudocelli) with simultaneous development of an 'apical organ'at the tip of the antennae. The obvious divergence from any existing Collembola families was additionally supported by the extremely prolonged intermoulting period as well as by the low strap value it showed with Onychiuridae as obtained by 18S DNA sequence analysis. All these were considered Justifying the creation of a new family, Gulgastruridae, but still revealing more allied to Onychiuvidae rather than to Hypogastruridae.

• 한국정보과학회논문지:소프트웨어및응용
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• 제27권3호
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• pp.290-301
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• 2000

일반적으로 형태소 분석 방법은 실행시에 매 어절마다 코드 변환, 형태소 분리, 원형 복원 규칙 적용을 통한 분석 후보 생성, 사전 탐색을 통한 분석 후보의 여과 등의 절차를 거쳐 형태소 분석을 수행하기 때문에 실행 효율의 관점에서 효율적이지 못하다. 이러한 문제점을 완화시키기 위해 도입된 어절단위 기분석 사전에 의한 분석은 실행시 처리에 소요되는 계산 부하를 크게 줄일 수는 있지만 어절의 수가 사실상 무한하므로 사전의 크기 문제 때문에 완전한 처리 방법이 될 수 없다는 단점이 있다. 본 논문에서는 이상과 같은 문제점을 해결하기 위해 두가지 대비되는 방법론을 결합하여 부분 어절별로 기분석 결과를 구축하여 형태소를 분석하는 방법에 대해 기술하고 있다. 이 방법에 의하면, 형태소 분리, 원형 복원 등 형태소 분석에 필요한 계산의 대부분을 실행시에 행하지 않고 기분석 결과의 구축 시에 행함으로써 실행시 계산 부하를 크게 줄일 수 있고 불필요한 중간 결과가 생성되지 않아 사전 탐색 횟수가 크게 절감되는 효과가 있으므로, 실행 효율을 크게 개선할 수 있다. 아울러 음소별 연산을 하지 않으므로 코드 변환 등에 소요되는 계산량도 전혀 필요치 않다는 특징도 있다.

• 말소리와 음성과학
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• 제4권3호
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• pp.129-136
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• 2012

Korean alveolar fricatives are late-developing speech sounds. Most previous research on phonemes used individual words or pseudo words to produce sounds, but word-level phonological analysis does not always reflect a child's practical articulation ability. Also, there has been limited research on articulation development looking at speech production by grammatical morphemes despite its importance in Korean language. Therefore, this research examines the articulation development and phonological patterns of the /s/ phoneme in terms of morphological types produced in children's spontaneous conversational speech. The subjects were twenty-two typically developing 3- and 4-year-old Koreans. All children showed normal levels in three screening tests: hearing, vocabulary, and articulation. Spontaneous conversational samples were recorded at the children's homes. The results are as follows. The error rates decreased with increasing age in all morphological contexts. Also, error percentages within an age group were significantly lower in lexical morphemes than in grammatical morphemes. The stopping of fricative sounds was the main error pattern in all morphological contexts and reduced as age increased. This research shows that articulation performance can differ significantly by morphological contexts. The present study provides data that can be used to identify the difficult context for articulatory evaluation and therapy of alveolar fricative sounds.