ALGAE

The Korean Society of Phycology (한국조류학회(藻類))
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Morphology and Molecular Data for Antarctic Cryophilic Microalga, Porosira pseudodenticulata

남극 호냉성 미세조 Porosira pseudodenticulata의 형태와 분자적 자료

  • 정웅식 (한국해양연구원 부설 극지연구소) ;
  • 주형민 (한국해양연구원 부설 극지연구소) ;
  • 홍성수 (한국해양연구원 부설 극지연구소) ;
  • 강재신 (한국해양연구원 부설 극지연구소) ;
  • 최한구 (한국해양연구원 부설 극지연구소) ;
  • 강성호 (한국해양연구원 부설 극지연구소)
  • Published : 2006.06.30
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Abstract

We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.

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References

  1. 강성호, 강재신, 이상훈, 김동선, 김동엽. 2000. 전 지구 환경변화에 대한 극지 식물 플랑크톤의 중요성. Korean J. Environ. Biol. 18: 1-20
  2. 남미라, 이옥민. 2001. 물먼지말 Staurastrum속 식물의 rbcL 유전자의 염기서열과 형태적 형질의 비교 연구. Algae 16: 363-367
  3. 해양수산부. 2005. '04 극지 생물 활용연구 보고서. pp. 135-156
  4. Bouquet J., Strauss S.H., Doerksen A.H., and Price R.A. 1992. Extensive variation in evolutionary rate of rbcL gene sequences among seed plants. Proc. Natl. Acad. Sci. USA 89: 7844-7848
  5. Draisma S.G.A., Reine W.F.P.V., Stam W.T. and Olsen J.L. 2001. A reassessment of phylogenetic relationships within the Phaeophyceae based on rubisco large subunit and ribosomal DNA sequences. J. Phycol. 37: 586-603 https://doi.org/10.1046/j.1529-8817.2001.037004586.x
  6. Kang J.S., Kang S.H. and Lee J.H. 1999. Cryophilic diatoms Navicula glaciei and N. perminuta in Antarctic coastal environment I. Morphology and Ecology. Algae 14: 169-179
  7. Kuhn S., Lange M. and Medlin L.K. 2000. Phylogenetic position of Cryothecomonas inferred from nuclear-encoded small subunit ribosomal RNA. Protist 151: 337-345 https://doi.org/10.1078/S1434-4610(04)70032-2
  8. Kuhn S., Medlin L. and Eller G. 2004. Phylogenetic postion of the parasitoid nanoflagellate Pirsonia inferred from nuclear-encoded small subunit ribosomal DNA and a description of Pseudopirsonia n. gen. and Pseudopirsonia mucosa (Drebes) comb. nov. Protist 155: 143-156 https://doi.org/10.1078/143446104774199556
  9. Lindstrom S.C. and Fredericq S. 2003. rbcL gene sequences reveal relationships among north-east Pacific species of Porphyra (Bangiales, Rhodophyta) and a new species, P. aestivalis. Phycological Research 51: 211-224 https://doi.org/10.1111/j.1440-1835.2003.tb00189.x
  10. Maddison D. R. and Maddison W. P. 2003. MacClade, version 4.06, Sinauer Associated, Inc., Sunderland, Massachusetts
  11. McCourt R.M., Karol K.G., Bell J., Helm-Bychowski K.M., Grajewska A., Wojciechowski M.F. and R.W. Hoshaw. 2000. Phylogeny of the conjugating green algae (Zygnemophyceae) based on rbcL sequences. J. Phycol. 36: 747-758 https://doi.org/10.1046/j.1529-8817.2000.99106.x
  12. Medlin L.K., Kooistra W.H.C.F., Gersonde R. and Wellbrock U. 1996. Evolution of the diatoms (Bacillariophyta). II. Nuclear-encoded small-subunit rRNA sequence comparisons confirm a paraphyletic origin of the centric diatoms. Mol. Biol. Evol. 13: 67-75 https://doi.org/10.1093/oxfordjournals.molbev.a025571
  13. Muller K.M., Sheath R.G., Vis M.L., Crease T.J. and Cole K.M. 1998. Biogeography and systematics of Bangia (Bangiales, Rhodophyta) based on the Rubisco spacer, rbcL gene and 18S rRNA gene sequences and morphometric analyses. I.North America. Phycologia 37: 195-207 https://doi.org/10.2216/i0031-8884-37-3-195.1
  14. Posada D. and Crandall K.A. 1998. Modeltest: testing the model of DNA substitution. Bioinformatics 14: 817-818 https://doi.org/10.1093/bioinformatics/14.9.817
  15. Rozen S. and Skaletsky H.J. 2000. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S. and Misener S. (eds), Bioinformatics Methods and Protocols: Methods in Molecular Biology. Humana Press. Totowa, NJ.,pp. 365-386
  16. Saunders G.W. and Kraft G.T. 1994. Small-subunit rRNA gene sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). I. Evidence for the Plocamiales ord.nov. Can. J. Bot., 72: 1250-1263 https://doi.org/10.1139/b94-153
  17. Savolainen V. and Chase M.W. 2003. A decade of progress in plant molecular phylogenetics. TRENDS in Genetics 19: 717-724 https://doi.org/10.1016/j.tig.2003.10.003
  18. Scott F.J. and Marchant H.J. 2005. Antarctic marine protists. ABRS, Canberra and AAD, Hobart. pp. 87-89
  19. Swofford D. L. 2002. $PAUP^{*}$. Phylogenetic Analysis Using Parsimony ($^{*}and$ Other Methods). version 4. Sinauer Associates, Sunderland, Massachusetts
  20. Thomas L., Torsten M., Hau U.L. and G nter F. 2000. Taxonomy and biophysical properties of cryophilic microalgae and their environmental factors in northwest Spitsbergen, Svalbard. 57th Eastern Snow Conference, Syracuse, New York, USA
  21. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F. and Higgins D.G. 1997. The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nuc. Acids Res. 24: 4876-4882
  22. Tomas C.R. 1997. Identifying Marine Phytoplankton. Part I.Academic Press, San Diego. pp. 41-43

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