• Title/Summary/Keyword: Korean cucumber-hot pepper

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Purification of a New Elicitin from Phytopthora cambivora KACC40160 (Phytophthora cambivora KACC 40160으로부터 새로운 elicitin의 분리)

  • Yoon, Sang-Hong;Bae, Shin-Chul;Park, In-Cheol;Koo, Bon-Sung;Kim, Young-Hwan;Yeo, Yun-Soo
    • Applied Biological Chemistry
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    • v.46 no.2
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    • pp.79-83
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    • 2003
  • Elicitins, proteinaceous elicitors secreted from Oomycetes fungi (Phytophthora spp. and Pythium spp.), have been known as inducer of hypersensitive response (HR) in incompatible interactions between plant and pathogens. Five elicitins among many Korean Phytophthora species caused the reactions of distal HR in radish, chinese cabbage and some hot pepper cultivars, but not in cucumber and tomato. Because the isolation of elicitin from Phytophthora cambivora hasn't been reported yet, we have purified a cambivorein, a new member of the elicitin family, from the culture filtrate of Phytophtilora cambivora (KACC 40160) by using FPLC (Fast Protein Liquid Chromatography, AKTA) with sepharose S and Sephacryl HR columns. We confirmed that it induces necrosis activities in some hot pepper cultivars and its molecular weight is about 10 KDa by Tricine-SDS-PAGE. Comparison of amino acid sequences of its N-terminal ends also informed the identification of Iysine at the 13th position, which is characteristic of a kind of basic elicitin isoform $({\beta}-isoform)$. It Also showed that our elicitin is not identical with N-terminal sequences of many elicitins reported from Phytophthora spp..

The Usage Status of Environment-friendly Materials for Vegetable Cultivated under Protected Houses in the Southern Part of Korea (남부지역 시설채소 재배 농가의 친환경농자재 사용실태)

  • Lee, Mi-Gyung;Hwang, Jae-Moon;Lee, Su-Rae
    • Korean Journal of Environmental Agriculture
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    • v.25 no.1
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    • pp.93-103
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    • 2006
  • This study was undertaken to survey the usage of environment-friendly materials for 8 vegetable crops including cucumber, tomato, strawberry, eggplant hot pepper, lettuce, perilla leaves, and Welsh onion, cultivated under protected houses. Total of 78 environment-friendly growers in the Southern part of Korea were surveyed from 2003 to 2004. The number of materials used for a crop based on the effectiveness claimed by farmers was $21{\sim}87$ for pest control, $0{\sim}9$ for fertilizing, $2{\sim}41$ for growth promotion and $10{\sim}37$ for soil improvement. It is needed to clarify the ingredients and effectiveness of those environment-friendly materials reaching 323 items, on a science-basis in the future.

Plant Growth Promoting Fungi Isolated from Rhizosphere of Zoysiagrass in Korea (잔디 근권에서 분리한 식물생장촉진 균류)

  • Park, Myung-Soo;Yu, Seung-Hun
    • The Korean Journal of Mycology
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    • v.33 no.1
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    • pp.30-34
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    • 2005
  • Fifteen PGPF (plant growth promoting fungi) isolates were selected from 728 fungal isolates collected from rhizosphere of zoysiagrass in Korea. Identification of the 15 isolates was based on their morphological characteristics. They were classified as Gliocladium sp. (n=1), Penicillium sp. (n=5), Trichoderma sp. (n=3), Fusarium sp. (n=3), and unidentifed species (n=3). Of the 15 isolates, six (PF-31, PF-136, PF-238, PF-255, PF-395, PF-420) significantly promoted the growth of tomato seedlings, and three (PF-31, PF-101, PF-255) also promoted the growth of hot pepper and two (PF-31, PF-225) also promoted the growth of cucumber, The 15 PGPF isolates were divided into 4 groups based on root colonizing ability. Isolates PF-17, PF-101 and PF-225 were included in the group 1, which had high root colonizing ability.

Distribution of Micronutrients in Plastic Film House Soils of Yeongnam Provincen (영남지역 시설재배지 토양의 미량원소 함량 분포)

  • Chung, Jong-Bae;Kim, Bok-Jin;Ryu, Kwan-Sig;Lee, Seung-Ho;Shin, Hyun-Jin;Hwang, Tae-Kyung;Choi, Hee-Youl;Lee, Yong-Woo;Lee, Yoon-Jeong;Kim, Jong-Jib
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.214-223
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    • 2006
  • For better management of micronutrients in crop cultivation, the availability of micronutrients in the soils must be updated and evaluated as a first step. This study was conducted to investigate the distribution of micronutrients in soils of plastic film houses. Total 396 soil samples were collected from the plastic film houses of various crops in Yeongnam province (strawberry, 96; red pepper, 66; tomato, 74; oriental melon, 97; cucumber, 63). Total and available contents of B, Cu, Zn, Fe, and Mn in the soils were determined. Available B was extracted with hot water and available Cu, Zn, Fe, and Mn were extracted with 0.1 N HCl. Mean values of total contents of B, Cu, Zn, Fe, and Mn in the plastic film house soils were 25, 32, 74, 21,316, and $420mg\;kg^{-1}$, respectively. Total contents of micronutrients in the plastic film house soils were similar to those found in the open fields nearby, while they were different among the locations investigated. Mean contents extractable B, Cu, and Zn in the plastic film house soils were 2.1, 7.5, and $35mg\;kg^{-1}$, respectively. The contents of extractable B, Cu, and Zn in the plastic film house soils were higher than those found in the open fields nearby with exceptions of B in soils of strawberry and Cu in soils of red pepper and oriental melon. However, mean contents of extractable Fe and Mn in the plastic film house soils were 156 and $146mg\;kg^{-1}$, respectively, and the mean content of extractable Fe was much lower than that found in open fields nearby. The contents of extractable Zn, Fe, and Mn were higher than the sufficient levels for the crop requirements in most of the plastic film house soils investigated. Contents of extractable Cu in most soils of strawberry, tomato and cucumber cultivations were higher than the sufficient level. However, extractable Cu contents were below the sufficient level in about 30% of investigated soils of red pepper and oriental melon cultivation. Soils containing higher contents of extractable B than the sufficient level were relatively fewer in comparison to the other micronutrients.

Inhibitory Effects of PLM-WE1 Formulated from Extract of Phellinus linteus Mycelium against Plant Viruses Infection and Identification of Active Compound (목질진흙버섯(Phellinus linteus) 균사체 추출물 제제 PLM-WE1의 식물 바이러스에 대한 감염억제 효과 및 활성성분의 동정)

  • Kwon, Soon-Bae;Bae, Seon-Hwa;Choi, Jang-Kyung;Lee, Sang-Yong;Kim, Byung-Sup;Kwon, Yong-Soo
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.259-265
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    • 2010
  • Pepper mild mosaic virus(PMMoV) and Cucumber mosaic virus (CMV) are important pathogens in various vegetable crops worldwide. We have found that hot water extract of Phellinus linteus mycelium strongly inhibit PMMoV and CMV infection. Based on these results, the inhibitor named as 'PLM-WE1' formulated from extract of Phellinus linteus mycelium was tested for its inhibitory effects on PMMoV and CMV infection to each local lesion host plant (Nicotiana glutinosa: PMMoV, Chenopodium amaranticolor: CMV). Pretreatment effect of PLM-WE1 against infections of each virus (PMMoV and CMV) to local host plant was measured to be 99.2% to PMMoV and 80.3% to CMV, and its permeability effect was measured to be 45.0% to PMMoV and 41.9% to CMV. Duration of inhibitory activity of PLM-WE1 against PMMoV infection on N. glutinosa was maintained for 3 days at 75% inhibition level and CMV infection on C. amaranticolor maintained for 3 days at 62% inhibition level. Inhibitory effects on systemic host plants of PLM-WE1 were measured to be 75~85% to PMMoV and 75% to CMV. Under electron microscope, PMMoV particles were not denatured or aggregated by mixing PLM-WE1. It is suggested that the mode of action of PLM-WE1 differ from that of inactivation due to the aggregation of viruses. The methanol extract of P. linteus mycelium was sequentially partitioned with haxane, ethyl acetate, BuOH and $H_2O$. The $H_2O$ fraction was showed high activity than the other fractions. The active compound was isolated with a partial acid hydrolysis, fractional precipitation with ethanol. The inhibitory effect of the precipitate isolated from 70% ethanol fraction was 99.1% to PMMoV and 88.0% to CMV. The structure of isolated compound was determined by $^1H$-NMR and $^{13}C$-NMR. This compound was identified as a polysaccharide consisting alpha or beta-glucan.

Traditional Jeupjang - A Study on Traditional Jeupjang (Succulent Jang) - (전통즙장 - 전통 즙장에 대한 연구 -)

  • Ann, Yong-Geun;Moon, Young-Ja
    • The Korean Journal of Food And Nutrition
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    • v.28 no.5
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    • pp.835-848
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    • 2015
  • In the past, Korea had many kinds of jeupjang (succulent jang), a rapidly maturing original Korean jang (fermented soybean paste) of which there is no record in Chinese cookbooks. However, this local delicacy has almost been forgotten. Therefore, we looked for information about jeupjang in cookbooks written prior to the Joseon Dynasty in Korea (1392~1910) and in the 1950s. Among the recipes, there were 34 jeupjangs prepared with vegetables, such as eggplant and cucumber, and 9 without. The main ingredients of jeupjang are soybean, bran (wheat crust), and barley, and wild wheat is also used. Jeupjang is made in small portions to expedite its rapid maturation, but the most common form is egg-shaped, and there is also a flat or round, hilt-shaped version. In most cases, jeupjang consists of a mixture of meju powder (moldy soybean), water, and salt. Other ingredients can include nuruk (moldy bran), bran, wheat flour, an alcoholic beverage, maljang (dried fermented soybeans), ganjang (liquid soy sauce), malt, and takju (Korean murky wine). Jeupjang meju can be fermented in a vessel, most widely in baskets made of straw (sum and dungumi) or willow or interwoven twigs (chirung), but jars can also be used. The leaves of the paper mulberry are generally used for the mat and cover, but straw or leaves of the sumac, mulberry, or pine tree, soy, and fallen leaves are also used. Unlike other jangs, jeupjang is matured at $60^{\circ}C$ to $65^{\circ}C$, using heat emitted from the decomposition of horse dung, haystacks, or manure. Jeupjang became defunct or was transformed into jeomjang, jiraejang, mujang, paggeumjang, makjang, jipjang, and tojang. These jangs differ from jeupjang in that they use rice, malt, or hot pepper powder.

The Usage Status of Pesticides for Vegetables under Greenhouse Cultivation in the Southern Area of Korea (남부지역 시설채소 재배 농가의 농약 사용실태)

  • Lee, Mi-Gyung;Hwang, Jae-Moon;Lee, Su-Rae
    • The Korean Journal of Pesticide Science
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    • v.9 no.4
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    • pp.391-400
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    • 2005
  • This study was undertaken to survey the pesticides used under the greenhouse cultivation of 9 vegetable crops including cucumber, tomato, strawberry, eggplant, hot pepper, paprika, lettuce, perilla leaves and green onion in the Southern area during the period of 2003-2004. The total number of farmers investigated was 202 which consisted of 124 conventional growers and 78 environment-friendly growers. The farmers were requested to record with respect to the crop's name, cultivation acreage, growing practices (conventional/environment-friendly), use purpose, pesticide kinds, usage quantity and application time of pesticides. The average usage quantity of pesticides for the 9 crops was 3.30 kg ai/ha in conventional growing and 0.47 kg ai/ha in environment-friendly growing. The average application time of pesticides was 11 times in conventional and 2 times in environment-friendly growing, with more than 90% cases as a foliar application. The number of active ingredients of pesticides was 34 in conventional and 10 in environment-friendly growing on the average per crop. The purpose of pesticide application was as a fungicide in 56% cases and as an insecticide in 43% cases.

Density of Arbuscular mycorrhizal spore of plastic film house soil in Yeongnam area and characterestics of AMF in vitro (영남지역 시설재배지에 분포하는 Arbuscular 균근균의 포자 밀도 및 기내조건에서의 포자발아와 균사생장 특성)

  • Park, Hyang-Mee;Nam, Min-Hee;Kang, Hang-Won;Lee, Jae-Saeng;Ko, Jee-Yeon;Kang, Ui-Gum;Park, Kyeong-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.2
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    • pp.203-209
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    • 1999
  • This study was conducted to obtain the basic data on agricultural use of arbuscular mycorrhizal fungi(AMF) in salt accumulated plastic film house soil by evaluating the density of AMF spores in plastic film house in Yeong Nam area and surface sterility condition, germination rate of AMF spores, and hyphal growth in vitro. The density of AMF spores in plastic film house soils was highest in the site of water melon, and those of cucumber, melon, hot pepper sites were followed in order. The number of AMF was in the range of 101-207 per 100 g dry soil. With decreasing the ratio of bacteria to fungi(B/F), the population density of AMF was increased, and available $P_2O_5$ content of soil was significantly correlated to the population densities of AMF($r=0.416^*$). The surface sterility rate and spore germination of AMF isolated in plastic film house soil were more than 50% in 2% chloramin T and 2% chloramin T + antibiotic and 0.5% NaOCl treatments. The germination rate of Gigaspora margarita in the range of initial pH 5~9 of the medium was more than 56%. Hyphal growth was increased as pH of the medium increased. However the germination rate of Acaulospora spinosa was highest in the medium of pH 9, and hyphal growth in vitro was poor and not related to pH of the medium.

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Estimation of the Chitinolytic and Antifungal Activity of Streptomyces sp. CA-23 and AA-65 isolates Isolated from Waste Mushroom Media (버섯 폐배지로부터 분리한 방선균 균주 CA-23과 AA-65균주의 키틴 분해능력과 항균력 검정)

  • Shim, Chang-Ki;Kim, Min-Jeong;Kim, Yong-Ki;Jee, Hyeong-Jin;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Kim, Seuk-Chul
    • The Korean Journal of Pesticide Science
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    • v.19 no.4
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    • pp.402-410
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    • 2015
  • The purpose of this study was to estimate the chitinolytic and antifungal activity of Actinomycetes sp.isolated from waste mushroom media. In five kinds of waste mushroom media, Sinyeong mushroom and Yangsongi were the order of the population density of actinomycetes. Totally 91 chitinolytic isolates of Actinomycetes sp. were obtained from waste mushroom media. The isolates were categorized into 3 groups based on chitinolytic activity and antagonisms against Phytophthora capsici, Rhizoctonia solani, Sclerotinia sclerotiorum, Collectotrichum gloeosporioides, and Cladosporium cucumerinum in vitro. CA-23 was selected as a representative isolate of a group showing strong chitinolytic and antagonistic activities to all of the plant pathogens, while AA-65 was selected as a representative isolate showing no chitinolytic activities but strong antagonistic activities to the pathogens. CA-23 and AA-65 were highly effective on control of Phytophthora blight of hot-pepper, powdery mildew and scab of cucumber in a greenhouse tests. Among the isolates tested, CA-23 showed highest control efficacy, while AA-65 not only effectively controlled the diseases but also consistently increased plant growth and yield. Although the isolates are similarly affected on suppression of plant pathogens, the isolates could be differ from each other in modes of action. Further studies on mechanisms and practical applications are being progressed.

Effects of UV-B and Growth Inhibitor on Physiological Changes and Antioxidat Enzyme Activities in Fruit Vegetable Plug Seedlings (UV-B와 생장억제 처리가 과채류 플러그묘의 생리 변화 및 항산화 효소의 활성에 미치는 영향)

  • Kwon, Joon-Kook;Kang, Nam-Jun;Lee, Jae-Han;Choi, Young-Ha;Yu, In-Ho;Cho, Mi-Ae;Kim, Hoe-Tae
    • Journal of Bio-Environment Control
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    • v.12 no.4
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    • pp.259-265
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    • 2003
  • The physiological changes and antioxidant enzyme activities of plug seedlings irradiated with 4 kJ${\cdot}m^{-2}{\cdot}d^{-1}$ UV-B or sprayed growth inhibitors of 50 ${\cdot}L^{-1}$ diniconazole and 500 mg${\cdot}L^{-1}$ hexaconazole for retarding the overgrowth of cucumber, tomato and hot pepper seedlings in summer season were investigated. Photosynthetic rate just after UV-B irradiation was significantly low but recovered to the level of non-treated seedlings 10 days after IV-B irradiation. And photosynthetic rate of chemical-sprayed seedlings was slightly lower in tomatoes but not different in cucumbers and peppers, compared with non-treated seedlings. Cholrophyll contens in the leaves showed no difference among treatments. Anthocyanin contents in the leaves of UV-B-irradiated seedlings were higher and no difference inchmical-sprayed seedlings, compared with non-treated seedlings. the activites of SOD(superoside dismutase), CAT )catalase), and POD(perosidase) of UV-B-irradated seedlings were higher thatn those of the non-irradiated seedlings just after UV-B irradiation. However. 10 days after UV-B irradiation, the activities were decreased to half of the value just after UV-B irradiation Whereasd the activies of chemical-sprayed seedlings were slightly higher than those of non-treated ones but lower than those of UV-B-irradiated one.