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Transcriptome analysis of a medicinal plant, Pistacia chinensis

  • Choi, Ki-Young;Park, Duck Hwan;Seong, Eun-Soo;Lee, Sang Woo;Hang, Jin;Yi, Li Wan;Kim, Jong-Hwa;Na, Jong-Kuk
    • Journal of Plant Biotechnology
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    • v.46 no.4
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    • pp.274-281
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    • 2019
  • Pistacia chinensis Bunge has not only been used as a medicinal plant to treat various illnesses but its young shoots and leaves have also been used as vegetables. In addition, P. chinensis is used as a rootstock for Pistacia vera (pistachio). Here, the transcriptome of P. chinensis was sequenced to enrich genetic resources and identify secondary metabolite biosynthetic pathways using Illumina RNA-seq methods. De novo assembly resulted in 18,524 unigenes with an average length of 873 bp from 19 million RNA-seq reads. A Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation tool assigned KO (KEGG orthology) numbers to 6,553 (36.2%) unigenes, among which 4,061 unigenes were mapped into 391 different metabolic pathways. For terpenoid backbone and carotenoid biosynthesis pathways, 44 and 22 unigenes encode enzymes corresponding to 30 and 16 entries, respectively. Twenty-two unigenes encode proteins for 16 entries of the carotenoid biosynthesis pathway. As for the phenylpropanoid and flavonoid biosynthesis pathways, 63 and 24 unigenes were homologous to 17 and 14 entry proteins, respectively. Mining of simple sequence repeat identified 2,599 simple sequence repeats from P. chinensis unigenes. The results of the present study provide a valuable resource for in-depth studies on comparative and functional genomics to unravel the underlying mechanisms of the medicinal properties of Pistacia L.

Meta-analysis on the effects of the prevention and intervention programs for internet addiction (인터넷 중독 예방 및 개입 프로그램의 효과에 대한 메타분석)

  • Oh, Ik-Soo;Kim, Chul
    • Journal of The Korean Association of Information Education
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    • v.13 no.4
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    • pp.529-537
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    • 2009
  • This study was to meta-analyze the effects of the internet addiction programs for elementary, junior, and high school students. The 54 programs to meet the conditions for meta-analysis were selected using internet search on the homepage of National Digital Library, KERIS, and National Assembly Library and the key words such as 'internet', 'addiction', 'counseling', and 'program' were used for searching the programs. The results of this study were followings: First, the average score of the effect sizes of 54 programs was 1.89 and thus the internet addiction programs had great positive effects on the improvement of adolescent's internet addiction. Second, all the programs' effect size scores showed more than 1.25 except psychological education program. Third, there were no significant differences at the effect size scores among elementary school students' goup, junior school student's group, and high school students' group. Fourth, when programs had more than 6 sessions, the programs showed desirable effect size. Fifth, when programs had were less than 15 subjects, the program's effect sizes were sufficiently great.

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Synthesis Technology of Functional Colloid Particles and Its Applications (기능성 콜로이드 입자의 제조기술 및 이의 응용)

  • Kang, Sung-Min;Choi, Chang-Hyung;Kim, Jongmin;Lee, Chang-Soo
    • Clean Technology
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    • v.18 no.4
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    • pp.331-340
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    • 2012
  • Synthetic methods of colloids have been significantly developed in industry due to their significant demand for preparation of functional particles. Recently, dynamic/static microfluidic system has emerged as a promising route to the synthesis of the particles, providing precise control of physical and chemical properties such as size, shape, porosity, surface roughness, and surface functionality. These formed particles can be potentially used in various applications including medical diagnostics, photonic device, and biological industry. In addition, these particles provide a novel route to create new materials via their directed self-assembly, and it enable to study and predict the natural phenomenon by mimicking of the nature. Therefore, we describe recent progress for functional colloid particles and its applications.

Design of electromagnetic type transducer to drive round window with high efficiency (고효율 전자기형 정원창 구동 트랜스듀서의 설계)

  • Lee, Jang-Woo;Kim, Dong-Wook;Kim, Myoung-Nam;Cho, Jin-Ho
    • Journal of Sensor Science and Technology
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    • v.19 no.6
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    • pp.449-455
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    • 2010
  • Implantable middle ear hearing devices(IMEHDs) have being actively studied to overcome the problems of conventional hearing aids. Vibration transducer, an output devices of IMEHDs, is attached on the ossicular chain and transmits mechanical vibration to cochlea. This approach allows us to hear more clear sound because mechanical vibration is effective to transfer high frequency acoustics, but occurs some problems such as fatigue accumulation to ossicular chian and reduction of vibration displacement caused by mass loading effect. Recently, many studies for the round window stimulation are announced, because it does not cause such problems. It have been studied by older transducers designed for attaching on ossicular chain. In this paper, we proposed a new electromagnetic transducer which consists of two magnets, three coils and a vibration membrane. The magnet assembly, magnet coupled in opposite direction, were placed in the center of three coils, and the optimum length of each coil generating maximum vibrational force was calculated by finite element analysis(FEA). The transducer was implemented as the calculated length of each coil, and measured vibration displacement. From the results, it is verified the vibration displacement can be improved by optimizing the length of coils.

Auto-dump Design of Postharvest Bulk Handling Machinery System for Onions

  • Park, Jongmin;Choi, Wonsik;Kim, Ghiseok;Kim, Jongsoon
    • Journal of Biosystems Engineering
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    • v.43 no.4
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    • pp.379-385
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    • 2018
  • Purpose: Postharvest handling of onions (harvesting, cleaning, grading, cooling, storing, and transport) should be performed continually to reduce costs and improve quality. The purpose of this study is to a) determine the design parameters and operating conditions of anion auto-dumping that constitutes a key component of the postharvest bulk handling machinery system, and b) to perform a performance test with the auto-dump prototype system. Methods: Kinematic analyses and computer simulations of the auto-dump mechanism were applied to analyze the operating conditions and design parameters. Results: The optimum working condition for the auto-dump was determined from kinetic analyses. In addition, the interaction between the velocity of the hydraulic cylinder and the angular velocity of the auto-dump were analyzed in order to control the bulk handling machinery system. The acting forces and optimum operating conditions of the hydraulic cylinder were determined by analyzing the forces related to the mass of inertia of the auto-dump assembly during rotation. The method of controlling the feeding rate of onions in terms of the uniformity of the stacking pattern and the control of the entire system was better than the two-stage method of controlling the rotational speed of the auto-dump. Based on the performance test with the prototype for the auto-dump, the stacking pattern and rigidity of the system were analyzed. Conclusions: These results would be of great importance in the postharvest bulk handling machinery system for onions.

Analytical Study on Improvement in Load Sharing for Planetary Gear Set using Floating Ring Gear

  • Chung, Woo-Jin;Choi, Kyujeong;Oh, Jooseon;Park, Young-Jun;Lee, Ki-Hun
    • Journal of Biosystems Engineering
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    • v.43 no.4
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    • pp.263-272
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    • 2018
  • Purpose: The load on the planet gear of a planetary gear set is uniformly distributed. However, manufacturing and assembly errors cause uneven load sharing in the planetary gear set. To solve this problem, most studies have suggested applying a floating sun gear to the planetary gear set. However, the effect of the floating ring gear and floating carrier has not been extensively studied. This study aimed to investigate the effect of the floating ring gear. Methods: Two models were developed; one was the fixed ring gear model, and the other was the floating ring gear model. In the fixed ring gear model, the clearance between the ring gear and the housing was $0{\mu}m$, and in the floating ring gear model, the clearance was from $10{\mu}m$ to $100{\mu}m$. The load sharing of the planetary gear set was evaluated by the load sharing factor. Results: Our study showed that with increase in clearance, the load sharing factor of the planetary gear set approached unity. In addition, when the clearance increased above a certain level by which a fully floating ring gear was achieved, the load sharing factor was not affected by the clearance. Conclusions: This indicates that the fully floating ring gear increased the power density of the planetary gearbox by uniformly dividing the load of the planetary gear set. For this reason, the size of the gearbox could be decreased by using a fully floating ring gear.

Formation of Reverse Cylindrical Micelles Using Mixtures of Lecithin and Salts (레시틴과 염들의 혼합물을 이용한 역실린더형 마이셀 형성)

  • Min-Seok Kang;Na-Hyeon Kim;Chang-Min Lee;Hee-Young Lee
    • Applied Chemistry for Engineering
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    • v.35 no.5
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    • pp.399-403
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    • 2024
  • Lecithin is one type of surfactant with amphiphilic properties, which forms spherical reverse micelles in various organic solvents. Salts with markedly low solubility in organic solvents can significantly increase their solubility through ionic interactions through the hydrophilic head of lecithin, leading to changes in the molecular morphology of lecithin. As a result, spherical reverse micelles can transform into cylindrical reverse micelles, which can cause abrupt rheological changes when these cylindrical reverse micelles form a transient network. This study precisely analyzes the formation of self-assembled structures and rheological changes by simultaneously adding two types of salts to a lecithin solution, using small-angle X-ray scattering analysis and rheometry.

Research Trends on Child Tea Ceremony Education (유아다례교육 연구의 동향분석)

  • Park, Young-Ja;Choi, Bae-Young
    • Journal of Family Resource Management and Policy Review
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    • v.14 no.3
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    • pp.143-163
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    • 2010
  • The purpose of this study is to analyze the trend of research system and contents of thesis which was published with a theme of child tea ceremony education. Under this purpose we have searched the thesis related to the child tea ceremony education which was in the data base of the National Assembly Library, the National Library of Korea, Korea Education & Research Information Service and we have collected total of 22 analyzing subject data. The major results of this study are as followings: First, after analyzing the research system, the release year of child tea ceremony education study which was published from year 2000 to 2009 are shown as followings, 1 unit in year 2000, 2 units in year 2001, 2002 & 2003, 4 units in year 2004, 5 units in year 2005, 2 units in year 2006, 1 unit in year 2007, 2 units in year 2008, 1 unit in year 2009. The publishing type were 14 units of master's degree thesis, 6 units of academic journal, 2 units of doctor's degree thesis. The academic area of researchers are 8 units of propriety and tea culture, 5 units of early childhood education, 5 units of child welfare, 2 units of family culture and consumer, 1 each unit of counseling psychology and korean culture. As per the research method there were 15 units of experimental study, 6 units of reference research, and 1 unit of thesis which adopts both interview and case study. Second, as a result of analyzing the research contents, the goal of child tea ceremony education was shown to be a holistic child growth and development, a formation of basic living habit, a development of pride on traditional culture and an establishment of national identity. The contents of child tea ceremony education has been classified into 57 items. The activity of child tea ceremony education has been classified into 34 items. The vitalizations of child tea ceremony education is shown to be a education for parents and teachers, a development of systematic educational program, durability of child tea ceremony education, and a verification of effectiveness of child tea ceremony education.

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Review of Meta-analysis Research on Exercise in South Korea (국내 운동 관련 메타분석 논문의 질 평가)

  • Song, Youngshin;Gang, Moonhee;Kim, Sun-Ae;Shin, In-Soo
    • Journal of Korean Academy of Nursing
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    • v.44 no.5
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    • pp.459-470
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    • 2014
  • Purpose: The purpose of this study was to evaluate the quality of meta-analysis regarding exercise using Assessment of Multiple Systematic Reviews (AMSTAR) as well as to compare effect size according to outcomes. Methods: Electronic databases including the Korean Studies Information Service System (KISS), the National Assembly Library and the DBpia, HAKJISAand RISS4U for the dates 1990 to January 2014 were searched for 'meta-analysis' and 'exercise' in the fields of medical, nursing, physical therapy and physical exercise in Korea. AMSTAR was scored for quality assessment of the 33 articles included in the study. Data were analyzed using descriptive statistics, t-test, ANOVA and ${\chi}^2$-test. Results: The mean score for AMSTAR evaluations was 4.18 (SD=1.78) and about 67% were classified at the low-quality level and 30% at the moderate-quality level. The scores of quality were statistically different by field of research, number of participants, number of databases, financial support and approval by IRB. The effect size that presented in individual studies were different by type of exercise in the applied intervention. Conclusion: This critical appraisal of meta-analysis published in various field that focused on exercise indicates that a guideline such as the PRISMA checklist should be strongly recommended for optimum reporting of meta-analysis across research fields.

An Empirical Study on Difference of Approval Rate for the Political Parties among Generations (정당 지지에 대한 세대별 차이 고찰)

  • Woo, Kyoungbong
    • Analyses & Alternatives
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    • v.4 no.2
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    • pp.103-132
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    • 2020
  • The purpose of this study is to observe whether intergenerational differences exist in support among major Korean political parties and, if so, how they exist, based on the results of the survey conducted nationwide. To achieve the purpose of the study, a questionnaire was prepared based on conjoint analysis, and the collected data was analyzed by applying a random parameter logit model. The main results of model analysis are summarized as follows. First, among the policy variables, statistically significant results were observed in the generation of 20s and 30s for the education variable. It was found that both 20s and 30s aimed for equal education at a higher level than other generations. Especially, the highest intensity aim for equal education culture was observed in the 20s. Second, the coefficients of major political parties were observed with a high level of statistical significance. This appears to be a result suggesting that voters decide on their voting behavior through thorough policy comparisons in addition to comprehensive consideration on various current issues. Third, a clear support for conservative parties was observed in the generation of 20s. A clear and intense distribution of preference for political parties classified as conservatives was observed in the 20s generation, which can be said to be mainly college students. This seems to be a profound founding related to the issue of "conservatization of the 20s," which has recently become a hot topic in Korean society. Fourth, a high level of support for progressive parties was observed in the 30s and 40s. The Justice Party can be classified as a minority party in the National Assembly House as of January 2019. Nevertheless, it was maintained at a relatively high level in national recognition, and it is presumed that the background was high level of support from the 30s and 40s. Fifth, a large level of standard deviation was observed in the preference for conservative parties in the 50s. This means that some respondents who are in their 50s or older strongly support the Liberty Korea Party, and some respondents in the same generation strongly disapprove it. Due to this countervailing power, it seems that the average support level for the Liberal Korean Party is low in the generations of 50s and older.?

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