• Journal of Animal Science and Technology
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• v.62 no.3
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• pp.423-426
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• 2020

Staphylococcus aureus is a significant pathogen that can source a variety of illness worldwide. In this announcement, we report here the complete genome sequence of S. aureus strain JDFM SA01, isolated from a milk filter collected from Korean dairy farm. The final complete genome assembly consists of one circular chromosome (2,748,925 bp) with an overall GC content of 32.9% and one circular plasmid sequence (24,655bp) with a GC content of 28.7%.

• Interdisciplinary Bio Central
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• v.1 no.1
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• pp.3.1-3.6
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• 2009

Introduction: GTPases known as translation factor play a vital role as ribosomal subunit assembly chaperone. The bacterial Obg proteins ($Spo{\underline{0B}}$-associated ${\underline{G}}TP$-binding protein) belong to the subfamily of P-loop GTPase proteins and now it is considered as one of the new target for antibacterial drug. The majority of bacterial Obgs have been commonly found to be associated with ribosome, implying that these proteins may play a fundamental role in ribosome assembly or maturation. In addition, one of the experimental evidences suggested that Bacillus subtilis Obg (BsObg) protein binds to the L13 ribosomal protein (BsL13) which is known to be one of the early assembly proteins of the 50S ribosomal subunit in Escherichia coli. In order to investigate binding mode between the BsObg and the BsL13, protein-protein docking simulation was carried out after generating 3D structure of the BsL13 structure using homology modeling method. Materials and Methods: Homology model structure of BsL13 was generated using the EcL13 crystal structure as a template. Protein-protein docking of BsObg protein with ribosomal protein BsL13 was performed by DOT, a macro-molecular docking software, in order to predict a reasonable binding mode. The solvated energy minimization calculation of the docked conformation was carried out to refine the structure. Results and Discussion: The possible binding conformation of BsL13 along with activated Obg fold in BsObg was predicted by computational docking study. The final structure is obtained from the solvated energy minimization. From the analysis, three important H-bond interactions between the Obg fold and the L13 were detected: Obg:Tyr27-L13:Glu32, Obg:Asn76-L13:Glu139, and Obg:Ala136-L13:Glu142. The interaction between the BsObg and BsL13 structures were also analyzed by electrostatic potential calculations to examine the interface surfaces. From the results, the key residues for hydrogen bonding and hydrophobic interaction between the two proteins were predicted. Conclusion and Prospects: In this study, we have focused on the binding mode of the BsObg protein with the ribosomal BsL13 protein. The interaction between the activated Obg and target protein was investigated with protein-protein docking calculations. The binding pattern can be further used as a base for structure-based drug design to find a novel antibacterial drug.

• Nuclear Engineering and Technology
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• v.54 no.7
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• pp.2670-2689
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• 2022

STREAM - a lattice transport calculation code with method of characteristics for the purpose of light water reactor analysis - has been developed by the Computational Reactor Physics and Experiment laboratory (CORE) of the Ulsan National Institute of Science and Technology (UNIST). Recently, efforts have been taken to develop a photon module in STREAM to assess photon heating and the influence of gamma photon transport on power distributions, as only neutron transport was considered in previous STREAM versions. A multi-group photon library is produced for STREAM based on the ENDF/B-VII.1 library with the use of the library-processing code NJOY. The developed photon solver for the computation of 2D and 3D distributions of photon flux and energy deposition is based on the method of characteristics like the neutron solver. The photon library and photon module produced and implemented for STREAM are verified on VERA pin and assembly problems by comparison with the Monte Carlo code MCS - also developed at UNIST. A short analysis of the impact of photon transport during depletion and thermal hydraulics feedback is presented for a 2D core also from the VERA benchmark.

• Korean Journal of Poultry Science
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• v.50 no.3
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• pp.187-191
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• 2023

Korean native ducks (KNDs) continue to have a high preference from consumers due to their excellent meat quality and taste characteristics. However, due to low productivity and fixed plumage color phenotype, it could not secure a large share in the domestic market compared to imported species. In order to improve the market share of KNDs, the genetic characteristics of the breed should be identified and used for improvement and selection. Therefore, this study was conducted to identify the genetic information of colored and white KNDs using next-generation resequencing data and screening for differences between the two groups. As a result of the analysis, the genetic variants that showed significant differences between the colored and white KND groups were mainly identified as mutations related to tyrosine activity. The variants were located in the genes that affect melanin synthesis and regulation, such as EGFR, PDGFRA, and DDR2, and these were reported as the candidate genes related to plumage pigmentation in poultry. Therefore, the results of this study are expected to be useful as a basis for understanding and utilizing the genetic characteristics of KNDs for genetic improvement and selection of white broiler KNDs.

• Nuclear Engineering and Technology
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• v.49 no.7
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• pp.1457-1462
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• 2017

Unsteady thermal processes in storage containers with spent nuclear fuel were modeled. The daily fluctuations of outer ambient temperatures were taken into account. The modeling approach, which is based on the solving of conjugate and inverse heat transfer problems, was verified by comparison of measured and calculated temperatures in outer channels. The time delays in the reaching of maximal temperatures for each spent fuel assembly were calculated. Results of numerical investigations show that daily fluctuation of outer temperatures does not have a large influence on the maximal temperatures of stored spent fuel, so that fluctuation can be neglected and only daily average temperature should be considered for safety estimation using the "best estimation" approach.

• Journal of Sensor Science and Technology
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• v.9 no.3
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• pp.208-212
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• 2000

A protein based vision biomembrane was fabricated by adsorbing bacteriorhodopsin into electrochemically polymerized polypyrrole film substrate mainly through strong electrostatic interaction. The immobilized bacteriorhodopsin on the polypyrrole film was demonstrated by SEM and SRET. The light signal transducing function from the bacteriorhodopsin which was adsorbed into Polypyrrole film was evaluated by electroretinogram(ERG). A wave form analysis of the electroretinogram indicated that the adsorbed bacteriorhodopsin retained its activity and light signal was obtained from the protein for at least one month.

• Applied Chemistry for Engineering
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• v.29 no.3
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• pp.253-257
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• 2018

DNA metallization has been emerged as a candidate for fabricating nanocircuits because of its simple process over a large area on a surface. With unique properties, DNA can be an excellent template to achieve molecular electronics. Thus, we introduced the preparation and properties of DNA metallization, and also suggested future directions in this review.

• Journal of Electrical Engineering and Technology
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• v.9 no.6
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• pp.2209-2217
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• 2014

A permanent-magnet-assisted switched reluctance motor (SRM) having small excitation poles, where phase coils are concentrically wound on the poles and thin permanent magnets are inserted inside the poles, is proposed in this paper. The insertion of permanent magnets into the stator excitation poles has a significant influence on positive torque improvement leading to a boost in efficiency. Three key design parameters such as the thickness of permanent magnets, space between two adjacent permanent magnets, and the width of stator excitation poles are determined during a design procedure in terms of the enhancement of positive torque. Step-by-step design modification and a comparison between the proposed permanent-magnet-assisted SRM and no-permanent-magnet SRM have been conducted by means of static torque comparison along with dynamic performance. The first prototype from steel laminations up to its physical assembly has been constructed.

• Journal of the Semiconductor & Display Technology
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• v.5 no.1 s.14
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• pp.5-11
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• 2006

One of the most important is to insure that a new circuit design is qualified far release before it is scheduled for manufacturing, test, assembly and delivery. Due to various causes, there happens to be a low yield in the wafer process. Wafer test is a critical process in analyzing the chip characteristics in the EDS(electric die sorting) using analytic tools -wafer map, wafer summary and datalog. In this paper, we propose new analytic map algorithms for DDI chip test data. Using the proposed analytic map algorithms, we expect to improve the yield, quality and analysis time.

• Design & Manufacturing
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• v.2 no.1
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• pp.11-14
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• 2008

we can save a development cost and time as computer was used in tool and die design of car fields in die manufacture process. Dimension accuracy errors such as springback, springgo, overcrown and twist were reduced product accuracy and caused trouble to assembly each parts of car. In this paper, CADCEUS was used to modify and optimize results of deflection for a tail gate panel of car parts in order to reduce dimension accuracy errors by springback in sheet metal forming. As CADCEUS was used to apply for a tail gate panel, the time for quality to improve was reduced to 30%.