• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.5
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• pp.51-58
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• 2010

This study was done to recommend a method for conserving the habitat of Amblyotropis verna grown naturally in campus of the Kyungpook National University at Daegu city. Vegetation characteristics and morphological characteristics of each individual were analyzed for the condition of the population at the growing season from May to June in 2010. Totally 11 vegetation data were collected and analyzed, and one vegetation community including Amblyotropis verna (Amblyotropis verna-Zoysia japonica community) was found. We found that the growing condition was not good with severe stamping and disturbance. The location-determining method by PCoA analysis showed the physical texture in soil was a critical factor determining the spatial distribution of the Amblyotropis verna-Zoysia japonica community. And the analysis of morphological characteristics found that light condition and soil hardness were the main ecological factor determining the size and form of each individual. Finally, this study recommend that minimizing impacts by human stamping and artificial disturbance and reducing the water stress were the best ways for the conservation of Amblyotropis verna habitats.

• ALGAE
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• v.21 no.2
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• pp.169-174
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• 2006

We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.

• Restorative Dentistry and Endodontics
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• v.47 no.3
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• pp.28.1-28.12
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• 2022

Objectives: Metalloproteinase-inhibiting agents, such as chitosan, can prevent collagen degradation in demineralized dental substrates, thereby improving the adhesive interface. This study evaluated the bond strength (BS) and chemical and morphological characterization of the adhesive interface after applying chitosan solution to demineralized dentin. Materials and Methods: The 80 third molars were selected. Forty teeth underwent caries induction using the pH cycling method. The teeth were divided according to the treatment: distilled water (control) and 2.5% chitosan solution. The surfaces were restored using adhesive and composite resins. Half of the specimens in each group were aged, and the other half underwent immediate analyses. The teeth were sectioned and underwent the microtensile bond strength test (µTBS), and chemical and morphological analyses using energy-dispersive spectroscopy and scanning electron microscopy, respectively. Data analysis was performed using 3-way analysis of variance. Results: For µTBS, sound dentin was superior to demineralized dentin (p < 0.001), chitosan-treated specimens had higher bond strength than the untreated ones (p < 0.001), and those that underwent immediate analysis had higher values than the aged specimens (p = 0.019). No significant differences were observed in the chemical or morphological compositions. Conclusions: Chitosan treatment improved bond strength both immediately and after aging, even in demineralized dentin.

• Korean Journal of Medicinal Crop Science
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• v.24 no.1
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• pp.55-61
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• 2016

Background : Plant breeding requires the collection of genetically diverse genetic resources. Studies on the characteristics of Platycodon grandiflorum resources have not been carried out so far. The present study was carried out to discriminate P. grandiflorum based on morphological characteristics and genetic diversity using simple sequence repeat (SSR) markers. Methods and Results :We collected 11 P. grandiflorum cultivars: Maries II, Hakone double white, Hakone double blue, Fuji white, Fuji pink, Fuji blue, Astra white, Astra pink, Astra blue, Astra semi-double blue and Jangbaek. Analyses of the morphological characteristics of the collection were conducted for aerial parts (flower, stem and leaf) and underground parts (root). Next, the genetic diversity of all P. grandiflorum resources was analyzed using SSR markers employing the DNA fragment analysis method. We determined that the 11 P. grandiflorum cultivars analyzed could be classified by plant length, leaf number and root characteristic. Based on the genetic diversity analysis, these cultivars were classified into four distinct groups. Conclusions : These findings could be used for further research on cultivar development using molecular breeding techniques and for conservation of the genetic diversity of P. grandiflorum. Moreover, the markers could be used for genetic mapping of the plant and marker-assisted selection for crop breeding.

• The Korean Journal of Mycology
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• v.47 no.2
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• pp.95-104
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• 2019

The genus Ochrolechia is a widespread, lichen genus in Korea. Despite being common, little is known about the species diversity and geographical distribution of Ochrolechia. In this study, we detailed the identification procedure of the genus Ochrolechia in a Korean collection and provided the description of each species. Using 104 specimens collected from 2003 to 2017, we identified four species of the genus Ochrolechia via morphological and/or molecular phylogenetic analysis: O. parellula, O. trochophora, O. yasudae and O. akagiensis. Among them, O. akagiensis had not been previously reported in Korea. Moreover, the species identified as O. frigida and O. tartarea in past studies were corrected as O. yasudae and O. parellula, respectively, based on morphological and/or molecular evidence. Phylogenetic analysis using the internal transcribed spacer regions including 5.8S rRNA gene showed that the four species separated clearly, indicating that the morphological identification corresponds to the phylogenetic identification. We provide a taxonomic key for the four species of the genus Ochrolechia.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.02a
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• pp.101-106
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• 2000

• Biomedical Science Letters
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• v.16 no.3
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• pp.187-192
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• 2010

The aim of this study was to compare the results of morphological phenotypic examination and molecular biological method, randomly amplified polymorphic DNA (RAPD) using clinical isolates from Korean patients infected with dermatophytes. A total of 44 clinical isolates have been collected by Korean Collection Medical Fungi (KCMF) and those were subjected for the RAPD analysis. The results showed that the fungal strains that have RG1 band patterns were identified as Trichophyton rubrum by the neighbour-joining analysis. Additionally, other fungal strains that have RG3 band patterns were identified as Epidermophyton floccosum and RG5 band patterns were identified as Micorsporum gypseum. Even though molecular biological method such as RAPD are not necessary, it might be useful when some strains have similar morphological characteristics or when same species has phenotypic variations. In this study, therefore, we targeted internal transcribed spacer (ITS) regions of fungal rDNA, performed RAPD and then compared with morphological phenotypic examination.

• Animal cells and systems
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• v.2 no.4
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• pp.451-454
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• 1998

Gulgastrura reticulosa, first described by Yosii (1966) as a monotypic new genus from a Korean limestone cave, was reviewed for its systematic position by c1adistic analysis of morphological characteristics, investigation of its intermaulting period and reproductive cycle as well as allozyme and 18S rDNA analysis. The great extent of divergence was strongly suggested by its combined lack of sensory organs (third antennal organ, postantennal organ, eyes, pseudocelli) with simultaneous development of an 'apical organ'at the tip of the antennae. The obvious divergence from any existing Collembola families was additionally supported by the extremely prolonged intermoulting period as well as by the low strap value it showed with Onychiuridae as obtained by 18S DNA sequence analysis. All these were considered Justifying the creation of a new family, Gulgastruridae, but still revealing more allied to Onychiuvidae rather than to Hypogastruridae.

• Journal of KIISE:Software and Applications
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• v.27 no.3
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• pp.290-301
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• 2000

Most morphological analysis methods require repetitive procedures of input character code conversion, segmentation and lemmatization of constituent morphemes, filtering of candidate results through looking up lexicons, which causes run-time inefficiency. To alleviate such problem of run-time inefficiency, many systems have introduced the notion of 'pre-analysis' of words. However, this method based on pre-analysis dictionary of surface also has a critical drawback in its practical application because the size of the dictionaries increases indefinite to cover all words. This paper hybridizes both extreme approaches methodologically to overcome the problems of the two, and presents a method of morphological analysis based on pre-analysis of partial words. Under such hybridized scheme, most computational overheads, such as segmentation and lemmatization of morphemes, are shifted to building-up processes of the pre-analysis dictionaries and the run-time dictionary look-ups are greatly reduced, so as to enhance the run-time performance of the system. Moreover, additional computing overheads such as input character code conversion can also be avoided because this method relies upon no graphemic processing.

• Phonetics and Speech Sciences
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• v.4 no.3
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• pp.129-136
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• 2012

Korean alveolar fricatives are late-developing speech sounds. Most previous research on phonemes used individual words or pseudo words to produce sounds, but word-level phonological analysis does not always reflect a child's practical articulation ability. Also, there has been limited research on articulation development looking at speech production by grammatical morphemes despite its importance in Korean language. Therefore, this research examines the articulation development and phonological patterns of the /s/ phoneme in terms of morphological types produced in children's spontaneous conversational speech. The subjects were twenty-two typically developing 3- and 4-year-old Koreans. All children showed normal levels in three screening tests: hearing, vocabulary, and articulation. Spontaneous conversational samples were recorded at the children's homes. The results are as follows. The error rates decreased with increasing age in all morphological contexts. Also, error percentages within an age group were significantly lower in lexical morphemes than in grammatical morphemes. The stopping of fricative sounds was the main error pattern in all morphological contexts and reduced as age increased. This research shows that articulation performance can differ significantly by morphological contexts. The present study provides data that can be used to identify the difficult context for articulatory evaluation and therapy of alveolar fricative sounds.