• The Korean Journal of Mycology
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• v.31 no.1
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• pp.44-49
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• 2003

Several microbial biocontrol products (Greenbiotech Co., Paju, Korea), Green-all T (Trichoderma harzianum), Green-all S(Bacillus sp.) and Green-all G (Streptomyces sp.) were supplemented with organic amendments such as sawdusts and rice hulls to study on efficacy of biological control of seeding damping-off of cucumber caused by Pythium ultimum. Sawdusts amended into potato dextrose agar alone could inhibit in vitro mycelial growth of P. ultimum. All there microbial products of Green-all T, Green-all G and Green-all S significantly reduced seeding damping-off (LSD, P=0.05). However, several amendments such as sawdusts and rice hulls into Green-all T and Green-all S products did not increase efficacy of biological control compared to non-amended treatment. In contrast, supplements of aminodoctor containing several amino acids (Greenbiotech Co., Korea) into Green-all G product significantly increased efficacy of biological control of seeding damping-off, resulting in from 42% to 2% disease incidence in relation to seedling emergence (LSD, P=0.05). Also, amendment of sawdusts into Tricoderma product significantly increased efficacy of biological control as disease index of 5.0 compared to non-amended control of 56.0 in Green-all T product alone. This indicates that organic amendments could increase efficacy of biological control of cucumber seedling damping-off.

• Journal of the Korea Organic Resources Recycling Association
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• v.17 no.3
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• pp.55-70
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• 2009

The long-term effects and the soil environmental changes were examined to ensure the safety of food waste compost in agricultural use. Based on conventional nitrogen application rate of chemical fertilizer, Pig manure compost with $24g\;N\;kg^{-1}$, $8g\;P_2O_5\;kg^{-1}$, and $10.4g\;K_2O\;kg^{-1}$ and food waste compost with $20g\;N\;kg^{-1}$, $20.1g\;P_2O_5\;kg^{-1}$, and $6.5g\;K_2O\;kg^{-1}$ were applied to the paddy soil in $2{\times}2{\times}2m$ lysimeter in which paddy rice (Oryza sativa L. var Chucheong) were grown. The rice grown where food waste compost applied showed better growth responses than control, whereas less yield rate than chemical fertilizer applied. The contents of organic matter, nitrogen, and phosphorus after experiment were increased with compost applied. In addition, it improved soil aeration by the application of food waste compost, while little difference was observed in the quality of surface, infiltrated, and ground water compared to chemical fertilizer applied or control.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.178-182
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• 2005

In the forthcoming 21C, the development of cultural lives depends on that the water demand will increase or not. On the opposite site of that circumstance, many factors of the small watersheds will influence directly on how to cover the surface of watersheds with land use, no planning developing watersheds, and the rearrangement of small rivers. Especially as the extraordinary climatic Phenomena, exhaust of $CO_2$ and destruction of 03 layer, water resource and water foresting content of the small watersheds will be decreased by confusing on the malting a plan of water resources. For example, those are Typhoon Rusa in 2002, Typhoon Maemi in 2003 and heavy storms in 2004. This study area has three group and one of them having three small watersheds, total five small watersheds. That is, Sabukmyeon small watersheds in Chuncheon, Three small watersheds in Wonju(Jeoncheon, Jupocheon and Hasunamcheon), and Suipcheon in Yanggu-Gun which are located far away each other three group and different precipitation data. According to the land use such as dry field(or farm), rice field, forest land. building site and others in small watersheds, the amount of runoff will be impacted by monthly precipitation. The comparison between the runoff was getting from Kajiyama Formula and calculated runoff from multi-linear regressed equations by land use Percentage was performed with different precipitation data and different small watersheds. Its correlations which are estimated by coefficient of correlation will be accepted or not, as approached 1.0000 values. As the monthly water resources amount is estimated by multi-linear regressed equations with different precipitation data and different small watersheds having no gauging station, we make a plan in order to demand and supply the water quantity from small river watersheds during return periods.

• Korean Journal of Agricultural Science
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• v.32 no.2
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• pp.107-126
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• 2005

The most limited production resource in Korea is land. During the period from 1995 to 2002, annual farm land area of 17,600ha have been converted to urban and industrial land. The self-sufficiency rate of rice, Korean staple food, is expected to be decreased from 97.5% in 2003 to 60-70% in 2020. Under such conditions, this study is aimed at first identifying multi-functional benefits of the reclamation projects such as agricultural production, industrial water supply, urban land supply, transportation effects, sightseeing effects and environmental values with and without the projects. To carry out the objectives, three existing tideland reclamation projects such as Daeho, Kumgang and Yongsangang irrigation project stage II were evaluated and Saemangeum tideland reclamation project which was jointly revaluated by environmental NGO and Govn't appointed specialists in 2000 was reviewed. According to this study results, tide land reclamation projects were showed financially and economically feasible and environmentally sustainable. The joint cost like estuary dam should be allocated based on the multi-functional benefits of the projects. To allocate the joint cost, legal and institutional improvement should adapt the joint cost allocation method as the specific cost-remaining benefit method. Korea has more than 402,000 ha of tidal flat of which 76,396ha have been reclaimed in 2003. To meet food security and to cope with shortage of land, phil-environmental reclamation projects should be continuously implemented and necessary tidal flats for protecting environmental ecosystem should be remained according to the detail survey results of reclaimable resources.

• Journal of Plant Biotechnology
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• v.33 no.3
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• pp.161-169
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• 2006

Plant metabolomics is a plant biology field for identifying all of the metabolites found in a certain plant cell, tissue, organ, or whole plant in a given time and conditions and for studying changes in metabolic profiling as time goes or conditions change. Metabolomics is one of the most recently developed omics for holistic approach to biology and is a kind of systems biology. For holistic approach, metabolomics frequently uses chemometrics or multivariate statistical analysis of metabolic profillings. In plant biology, metabolomics is useful to determine functions of genes often in combination with DHA microarrays by analyzing tagged mutants of the model plants Arabidopsis and rice. This review paper attempted to introduce basic concepts of metabolomics and practical uses of multivariate statistical analysis of metabolic profiling obtained by $^1$H HMR and Fourier transform infrared spectrometry.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.3
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• pp.199-213
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• 2020

Grain size and shape are the two important components contributing to rice yield and quality. To analyze traits related to grain-shape, a total of 272 varieties derived from japonica, japonica black and Tongil-type rice accession in Korea were evaluated in this study. The traits, grain length (GL), grain width (GW), grain thickness (GT), length to width ratio (RLW), and 1000-grain weight (TGW) were measured and replicated 10 times. Genes (GW2, GS3, qGL3, qSW5, GS5, TGW6, GW7, and GW8) related to grain-shape were validated in the accessions using specific DNA marker sets. K-mean clustering of the accession based on phenotypic data revealed three groups: group 1 was classified by GW and GT and included most of japonica type, group 2 was classified by RLW and GL reached a medium size and possessed a half spindle-shaped type, and group 3 was classified by TGW, reached a long size and possessed a semi-round shape. In validation tests using the marker sets, both gw2 and tgw6 were validated in less than 1% of the tested accessions and two allelic types, qgl3 and gw8, were only verified in Tongil-type accessions. For GW8 and GW2, any different amplicons were not amplified in any japonica or Tongil-type accessions, respectively. In order to suggest the representative grain-shape gene combinations for each ecotype, the allelic combinations were evaluated by PCR analysis. Cj1 and 2 in japonica (Cj1-7), Cj_b1 and 2 in japonica-black (Cj_b1-3), and CT3 in Tongil-type (CT1-13) turned out to be the dominant combination in each ecotype, respectively. In addition, the results revealed that introgression of four genes (gw2, gs3, qSW5, and GS5) would expand the diversity of grain shape in Korean japonica varieties. The gene combinations information could be utilized practically to understand or enhance grain shape in japonica rice breeding program.

• Journal of Agricultural Extension & Community Development
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• v.20 no.4
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• pp.1105-1147
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• 2013

To investigate farmers' satisfaction level and factors influencing on various agricultural and rural policies, an interview survey has been carried out in Jeonnam and Jeonbuk agricultural area, and collected 750 survey questionnaires from farmers. Satisfaction level was low in average ranging from 2.71 to 3.09 in five point Likert-scale on 22 agricultural-rural related policies. Ordered logit model results showed that satisfaction level decreased when farmers are older, had higher income, and had higher number of attendance in agricultural education programme. In addition, satisfaction level decreased when farms had main source of income from rice farming, dry-field farming, livestock farming, or facility horticulture. Lower satisfaction level was also related to location of farm. On the contrary, satisfaction level increased when the farmer had greater owned land. Among 22 agricultural and rural policies, practices that farmers prefer include Direct Payment for Rice Farming Income Compensation, Environment-Friendly Farming Service, Farmland Banking Services in order. Since direct payment policies that farmers prefer may not contribute in development of agriculture, policies can induce both farm income and agricultural development may increase farmer satisfaction level and finally resolve the income gap between the urban workers and farmers.

• Journal of the Korean Society of International Agriculture
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• v.30 no.4
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• pp.339-346
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• 2018

Cyanazine is a member of the triazine family of herbicides. Cyanazine is used as a pre- and post-emergence herbicide for the control of annual grasses and broadleaf weeds. This experiment was conducted to establish a determination method for cyanazine, as domestic unregistered pesticide, residue in major agricultural commodities using HPLC-DAD/MS. Cyanazine was extracted with acetone from representative samples of five raw products which comprised apple, green pepper, Kimchi cabbage, hulled rice and soybean. The extract was diluted with saline water and partitioned to dichloromethane for remove polar extractive in the aqueous phase. For the hulled rice and soybean samples, n-hexane/acetonitrile partition was additionally employed to remove non-polar lipids. The extract was finally purified by optimized florisil column chromatography. On a $C_{18}$ column in HPLC, cyanazine was successfully separated from co-extractives of sample, and sensitively quantitated by diode array detection at 220 nm. Accuracy and precision of the proposed method was validated by the recovery experiment on every major agricultural commodity samples fortified with cyanazine at 3 concentration levels per agricultural commodity in each triplication. Mean recoveries were ranged from 83.6 to 93.3% in five major representative agricultural commodities. The coefficients of variation were all less than 10%, irrespective of sample types and fortification levels. Limit of quantitation(LOQ) of cyanazine was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method using LC/MS with selected-ion monitoring(SIM) technique was also provided to clearly identify the suspected residue.

• Journal of Sericultural and Entomological Science
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• v.49 no.1
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• pp.18-23
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• 2007

The importance of genetic resources conservation and utilization for economy and the health and well-being of societies in general is recognized all over the world. Mulberry genetic resources contain many natural components and are considerable resource for functional sericulture. We carried out antioxidative capacity analysis of mulberry leaves that were collected from Korea and some of foreign countries and preserved in the greenhouse. The mean antioxidant capacity of 34 accessions was 892.30nmol(ascorbic acid equivalent). Also we evaluated fruity characteristics and selected 4 accessions as breeding resources for the production of mulberry fruit. To investigate the effect of tea on antioxidative capacity, five kinds of tea(coffee mix, green tea added brown rice, mulberry leaf tea, Polygonatum odoratum tea and black tea added lemon) were selected and analyzed. Their's antioxidative capacity were 2,531.01nmol, 1,867.42nmol, 1,053.72nmol, 292.71nmol and 188.91nmol, respectively. The antioxidative capacity of drinking water soaked with mulberry leaf showed 891.96nmol.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.71-77
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• 2015

Much attention has been focused on the activity of the natural antioxidants present in fruits and vegetables, because potentially these components may reduce the level of oxidative stress. Especially, mulberry leaves containing many natural components are considerable resource for natural antioxidants. The antioxidant capacity of mulberry leaves was investigated with minilum L-100 device and ARAW-KIT (anti-radical ability of water-soluble substance), in comparison to the ascorbic acid. The antioxidant capacity of 16 varieties was 3303.4 nmol at opening stage of five leaves in spring. The highest stage of antioxidant capacity (3708.0 nmol) and yield rate was just before the coloration stage with anthocyanin in fruits, whereas the lowest stage was middle of June (2231.6 nmol) and about two months growing stage after summer pruning (2064.6 nmol). But after summer pruning, the antioxidant capacity of mulberry leaves increased gradually until just before fallen leaves stage. Even if samples were same variety, antioxidant effect of those showed different results according to collected regions. Also, antioxidant effect of mulberry leaves were higher than that of branches. The antioxidant capacity of yield-type mulberry leaves and fruits (Morus alba L., M. bombycis Koidz, and M. Lhou (Ser.) Koidz) collected from In-je, Won-ju and Yang-yang regions, Kang-won province, Korea, was investigated. The results indicated that total antioxidant capacity of yield-type mulberry leaves was 2711.2 nmol. In the antioxidant capacity analysis of Jeollabuk-Do genetic resources, autumn's mulberry leaves showed higher antioxidant capacity than that of spring's it. To investigate the effect of tea on antioxidative capacity, five kinds of tea(coffee mix, green tea added brown rice, mulberry leaf tea, Polygonatum odoratum tea and black tea added lemon) were selected and analyzed. Their's anti-oxidative capacity were 2,531.01 nmol, 1,867.42 nmol, 1,053.72 nmol, 292.71 nmol and 188.91 nmol, respectively. The antioxidative capacity of drinking water soaked with mulberry leaf showed 891.96 nmol.