• Title/Summary/Keyword: Korea national arboretum

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The Induction of Somatic Embryogenic Callus from Petals-Derived Callus in Rosa hybrida (국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도)

  • Lee, Su Young;Shin, Ju Young;Lee, Young Ah;Ahn, Chang Ho;Kim, Yae Jin;Park, Pil Man;An, Hye Ryun;Lee, Ka Youn;Jung, Hyun Hwan
    • Korean Journal of Plant Resources
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    • v.35 no.5
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    • pp.652-658
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    • 2022
  • This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

Molecular phylogeny of Astilbe: Implications for phylogeography and morphological evolution (노루오줌속(Astilbe)의 분자 계통: 계통지리 및 형질 진화에 대한 고찰)

  • Kim, Sang-Yong;Kim, Sung-Hee;Shin, Hyunchur;Kim, Young-Dong
    • Korean Journal of Plant Taxonomy
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    • v.39 no.1
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    • pp.35-41
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    • 2009
  • Astilbe (Saxifragaceae) is a genus well known for its disjunctive distribution in Asia and eastern North America. In this study, we reconstructed a molecular phylogeny of the genus using the sequences of ITS regions of nuclear ribosomal DNA. A total of 17 species representing major lineage of Astilbe and closely related taxa were included in the phylogenetic analyses. We obtained a Bayesian phylogenetic tree in which Saxifragopsis was positioned as a sister group to Astilbe. The Japanese endemic species, A.platyphylla was the most basal lineage within the genus. This species is well known for its distinct morphological features such as unisexual flowers, apetaly, and calyx with 7-11 lobes. Two species, A. biternata, a New World representative of the genus, and A. rivularis widely distributed in S. Asia, branched off early in the evolution of Astilbe. The remaining species formed a strongly supported core clade, which diverged into two robust geographical lineages: the first ("Japonica" clade) of species distributed in Japan, Taiwan, and Philippines and the other ("Rubra" clade), of taxa in China and Korea. The ITS phylogeny indicates that the Bering land bridges were the major route for the origin and dispersal of A. biternata. The two Taiwanese taxa and A. philippinensis were found to derive from the Japanese member, as the genus advanced southwards. The ITS phylogeny suggests that apetaly originated independently at least two times within the genus. Our results do not support Engler's classification system of the genus based on the leaf type (simple vs. compound), but reaffirm Hara's taxonomic idea which primarily considered the features of calyx.

Growth Characteristics of One-year-old Container Seedlings of Pinus densiflora by Irrigation Level (관수수준에 따른 소나무 용기묘 1년생의 생장 특성)

  • Cha, Young-Geun;Choi, Kyu-Seong;Song, Ki-Seon;Sung, Hwan-In;Kim, Jong-Jin
    • Journal of Bio-Environment Control
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    • v.26 no.3
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    • pp.167-174
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    • 2017
  • To identify the appropriate irrigation level for Pinus densiflora, a common reforestation species in Korea, we investigated their growth response characteristics according to different irrigation treatment levels for producing container seedlings with relatively high growth rate for higher survival rate when planted at the reforestation site. The container seedlings including control seedlings (no irrigation was applied) were grown in 104-cell trays were irrigated for 8 weeks from 15 weeks after seeding, at intervals of 1, 2, 3, 5, 7, 10, and 15 days. Analysis of the height growth, root collar diameter growth, and dry matter production of the container seedlings according to irrigation showed that the highest growth reaction was observed for the irrigation interval of 1 day. A shorter irrigation cycle resulted in better growth of the container seedlings, but overall, longer total root length were observed with an irrigation cycle of 3 days compared with cycles of 1 or 2 days. Quality index (QI) was the highest for the irrigation interval of 1 day, and tended to decrease with increase in the irrigation interval. Thus, it was determined that irrigation at intervals of 1-2 days was appropriate for growing Pinus densiflora container seedlings.

Analysis of MODIS LAI and NDVI Patterns of Broad-leaved Trees by the Timesat Program on the Korean Peninsula (Timesat 프로그램에 의한 한반도 활엽수의 지역별 MODIS LAI 및 NDVI 패턴 분석)

  • Seo, Dae Kyo;Lee, Jeong Min;Lim, Ye Seul;Han, Sang Won;Pyeon, Mu Wook
    • Journal of Korean Society for Geospatial Information Science
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    • v.25 no.2
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    • pp.13-19
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    • 2017
  • This paper analyzed MODIS data from 2006 to 2013 to determine relationship between meteorological changes and vegetation index. The experimental area was divided into the northern, central and southern regions according to the regional characteristics, and the smoothed MODIS LAI and NDVI were obtained using Timesat. In the case of precipitation, MODIS NDVI had correlation coefficients of 0.66, 0.44 and 0.35 in the northern, central and southern regions and the correlation was the highest in the northern region. In the case of temperature, MODIS LAI had correlation coefficients of 0.66, 0.64 and 0.68, and MODIS NDVI had 0.89, 0.89 and 0.80. The correlation of MODIS NDVI was higher and showed similar positive correlation regardless of region. In addition, The accuracy between Timesat plant seasonal start and actual plant seasonal start in MODIS NDVI was higher than MODIS LAI. The average error in MODIS LAI was 19 days in the central region and 20 days in the southern region. And the average error in MODIS NDVI was 6 days in the central region and 8 days in the southern region.

Anti Inflammatory Activity of Viburnum dilatatum Thunb. Extract as Cosmetic Ingredient (화장품 소재로서 가막살나무 추출물의 항염증 효능)

  • Kwon, Yoo-Bin;Yoo, Byoung-Sam;Kim, Dae-Shin;Moon, Seong-Joon;Yoon, Moung-Seok;Park, Soo-Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.36 no.3
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    • pp.183-191
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    • 2010
  • The crude ethanol extracts and their solvent-partitioned fractions derived from the leaf and twig of Viburnum dilatatum Thunb. were investigated for their 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging efficacy. The results showed that the butanol-soluble fraction ($SC_{50}\;=\;110.30\;{\mu}g/mL$) exhibited higher anti-oxidant activity than the crude ethanol extract ($SC_{50}\;=\;117.03\;{\mu}g/mL$) in the DPPH assay model. Then, the effects of the same extract samples on the production of nitric oxide were examined in LPS-stimulated RAW264.7 cells. Although the hexane and methylene chloride-soluble fraction showed a weak anti-oxidant activity, they exhibited potent inhibitory activity of NO production above 50 % at a concentration of $10\;{\mu}g/mL$. The hexane-soluble fraction also showed the inhibitory effect on mRNA expression of pro-inflammatory mediators such an TNF-$\alpha$, IL-$1{\beta}$, IL-6, iNOS and COX-2 in LPS-stimulated RAW264.7. These results suggest that the solvent extracts of Viburnum dilatatum Thunb. could be used as an anti-irritation ingredient.

Studies on the Growth Characteristics of Convallaria keiskei Population (은방울꽃 개체군의 생장특성에 관한 연구)

  • Lee, Se-Ra
    • Korean Journal of Plant Resources
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    • v.20 no.4
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    • pp.292-297
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    • 2007
  • To explain the relationship between plant size and seed production, change of sexual organs, relationship between each organs of Convallaria keiskei population, which is located in Gwiyeo-2-ri, Namjong-myeon, Gwangju-si, Gyeonggi-do these were studied from April to August 2002. This study was carried out two ways. One way was to set up the permanent quadrat to explain the change of sexual organs. The other was to carry the collected sample to the laboratory and analysis it to explain the relationship between each organs and energy division of seed production. In the Convallaria keiskei population, the average number of leaf which blooming shoot in the permanent quadrat was 2 and I could find the late leaf size was larger. It was found that, per shoot, the average number of flower was 7.9, fruit was 5.4 and seed was 16.1. Each properties in the sexual organ, underground part size and leaf size, had correlation of 1% or within 5%. The flowering time of the Convallaria keiskei was from April 26 to May 11. Therefore, it can be estimated that the blooming period was $8{\sim}9$ days in just one flower. Among that flowers bloom at the beginning May, 80% fruit at the end of May, and 68% fruit at the end of August. The dry weight of seed had more correlation dry weight of underground part than leaf area.

Phylogenetic study of the section Adonanthe of genus Adonis L. (Ranunculaceae) based on ITS sequences (ITS 염기서열에 의한 복수초속 복수초절(미나리아재비과)의 계통분류학적 연구)

  • Son, Dong Chan;Park, Beom Kyun;Ko, Sung Chul
    • Korean Journal of Plant Taxonomy
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    • v.46 no.1
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    • pp.1-12
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    • 2016
  • DNA sequences of nrITS regions obtained from 49 accessions representing 12 species and one variety within the section Adonanthe of the genus Adonis were analyzed to test the previous intra-sectional classification system and to determine their phylogenetic relationships. The results showed that the seven accessions of A. amurensis Regel et Radde included in the present study did not form a monophyletic group, as some of the accessions showed a close relationship with the Japanese endemic species A. ramosa Franch., which implies that the current species delimitation and identification of A. amurensis is problematic. Adonis pseudoamurensis W. T. Wang, which is frequently misidentified as A. ramosa in Korea, formed a segregated group, which suggests that they should not be considered as conspecific taxa. Accessions from A. shikokuensis Nishikawa et Koji Ito, A. multiflora Nishikawa et Koji Ito, and A. pseudoamurensis formed a clade, but monophyly of each species was not evident. The nrITS data did not support the classification system proposed by Wang, who classified sect. Adonanthe into four series, as most of these were found to be either polyphyletic or paraphyletic.

The Flora of Vascular Plants in Mt. Chijae(Gamagol) and Its Adjacent Areas (in Damyang-gun, Jeonnam-do, Korea) (담양군 치재산(가마골) 일원 관속식물상)

  • Soon-Ho Shin;Kyoung-Pae Yun;Sang-Mi Kim;HyunSuk Kim
    • Korean Journal of Environment and Ecology
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    • v.38 no.1
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    • pp.22-47
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    • 2024
  • This study aims to reveal the distribution of native plants, rare plants, and specialty plants and provides basic data for efficient ecosystem management through a survey of the flora in Mt. Chijae (Gamagol) and its adjacent areas in Damyang-gun, Jeonnam-do, while also identifying useful plants in the eco-city Damyang-gun for distinctive garden plants. A total of 21 field surveys were conducted from March 2022 to June 2023 to collect and identify 492 taxa consisting of 101 families, 304 genera, 444 species, 10 subspecies, 35 varieties, and 3 formas. The analysis of the prepared sample list showed 14 taxa designated as specialty plants, 9 taxa designated as rare plants by the Korea Forest Service, 7 taxa designated as the national red (i.e., endangered) plants by the Ministry of Environment, and 68 taxa with phylogenetic specific species, of which 45 taxa for Grade I, 5 taxa for Grade II, 16 taxa for Grade III, and 2 taxa for Grade IV were confirmed. The list included 155 taxa of biological resources subject to export approval by the Ministry of Environment and 38 taxa of alien plants of which 7.7 percent were naturalized, and the urbanization index was 6.1 percent. Four taxa were ecosystem-disturbing invasive alien plants designated by the Ministry of Environment introduced artificially or naturally from abroad. This study is expected to contribute to creating unique and attractive landscapes in Damyang if the specific plants found in Mt. Chijae are grown and planted in city gardens.

Floristic study and conservation management strategies of algific talus slopes on the Korean peninsula (한반도 풍혈지의 관속식물상과 보전관리 방안)

  • Kim, Jin-Seok;Chung, Jae-Min;Kim, Jung-Hyun;Lee, Woong;Lee, Byoung-Yoon;Pak, Jae-Hong
    • Korean Journal of Plant Taxonomy
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    • v.46 no.2
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    • pp.213-246
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    • 2016
  • Algific talus slopes tend to occur on steep north-facing slopes with bedrock that retains ice and emits cold air throughout the growing season. Algific talus slopes provide a suitable microclimate for disjunct or relict populations of northern plant species at low altitude habitats in temperate zones. The purpose of this study is to suggest a strategy for the comprehensive conservation of the vegetation of algific talus slopes through studies of the floristics and plant species compositions and threat factors at present and in the future of 15 major algific talus slopes in Korea. As a result, the vascular plants surveyed on 15 major algific talus slopes were recorded, with a total of 587 taxa, 109 families, 323 genera, 531 species, 7 subspecies, 47 varieties 1 form and 1 hybrid. Of them, endemic plants numbered 26 taxa, and threatened species according to the IUCN valuation basis numbered 8 taxa. Fourth (IV) and fifth (V) degree indicator species as specified by floristic subregions numbered 31 taxa. Peculiarly, several subalpine-native plant species, in this case Cystopteris fragilis, Gymnocarpium dryopteris, Huperzia selago, Rosa koreana, Vaccinium vitis-idaea and Woodsia hancockii, were distributed on algific talus slopes at 100-600 m above sea level. Numerous and diverse biological resources native to algific talus slopes in Korea have been consistently disturbed or damaged by human activities without some form of protection. An all-taxa biodiversity inventory should be surveyed to provide more information about all biological species living on algific talus slopes. In addition, conservation strategies to ensure biodiversity and effective management of algific talus slopes are discussed in detail.

Growth Responses of Potted Gerbera 'Sunny Lemon' under Non-Nutrient Solution Recycling System by Media and Nutrient Contents (비순환식 분화 양액재배시 배지와 양액함량에 따른 거베라 'Sunny Lemon'의 생육반응)

  • Kil, Mi-Jung;Shim, Myung-Sun;Park, Sang-Kun;Shin, Hak-Gi;Jung, Jae-A;Kwon, Young-Soon
    • Journal of agriculture & life science
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    • v.45 no.6
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    • pp.73-80
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    • 2011
  • To investigate the characteristics of plant growth and flower quality of gerbera 'Sunny Lemon' by amount of nutrient solution, young seedling plants, 'Sunny Lemon' were transplanted to rock-wool and medium of peat moss and perlite mixed with 1 to 2 and they were acclimatized in greenhouse during about 1 month. Nutrient solution supplied to the plants is sonneveld solution of 1/2 concentration and treatments launched June 24, 2010 when average plant height was $20{\pm}1cm$. Nutrient contents as a standard for starting point of irrigation by time domain reflectometry (TDR) were determined with 60-65%, 70-75%, and 80-85%. Results of growth during vegetative growth, plant height, leaf width and leaf number increased by 10% in rockwool, but they were not significantly different. As for plant growth depending on nutrient content, 80-85% treatment showed the highest values. Leaf number increased by 60%, and leaf width and plant height had a about 40% increase than initial growth. Effectiveness for flower quality, yield and days to flowering were superior when nutrient content of media was higher than in the others. Especially, average days to flowering in 80-85% content was advanced by 7-10 days compared to the day in 60-65% treatment. The total amount of nutrient supply per plant was higher in mixed medium than in rockwool, but change patterns of EC and pH were enhanced in rockwool. Based on our results, we recommended that growth, cut flower, and yield of gerbera 'Sunny Lemon' were more effective when nutrient content of mixed medium was maintained at 80-85%.