Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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The Induction of Somatic Embryogenic Callus from Petals-Derived Callus in Rosa hybrida

국내 육성 장미 품종 꽃잎 유래 체세포배 발생 캘러스 유도

  • Lee, Su Young (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Shin, Ju Young (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Lee, Young Ah (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Ahn, Chang Ho (Garden Plant Nursery Team, Korea Institute of Arboretum Management) ;
  • Kim, Yae Jin (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Park, Pil Man (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • An, Hye Ryun (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Lee, Ka Youn (Floriculture Research Division, National Institute of Horticultural & Herbal Science) ;
  • Jung, Hyun Hwan (Floriculture Research Division, National Institute of Horticultural & Herbal Science)
  • 이수영 (국립원예특작과학원 화훼과) ;
  • 신주영 (국립원예특작과학원 화훼과) ;
  • 이영아 (국립원예특작과학원 화훼과) ;
  • 안창호 (국립세종수목원 정원소재연구실) ;
  • 김예진 (국립원예특작과학원 화훼과) ;
  • 박필만 (국립원예특작과학원 화훼과) ;
  • 안혜련 (국립원예특작과학원 화훼과) ;
  • 이가연 (국립원예특작과학원 화훼과) ;
  • 정현환 (국립원예특작과학원 화훼과)
  • Received : 2022.07.15
  • Accepted : 2022.09.06
  • Published : 2022.10.01
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Abstract

This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

화색이 다른 장미 3품종('Ice Wing', 'Orange Eye', 'Pink beauty') 꽃잎 절편체를 3종류(MS, SH, WPM)의 기본배지에 2, 4-D 11 mg/L를 첨가한 배지에 47일간 암배양하고 절편체로부터 캘러스 형성율을 조사한 결과, 모든 품종에서 꽃잎 절편체로부터 캘러스 형성이 관찰되었다. 또한 화색이 흰색인 'Ice Wing' 품종의 꽃잎을 기본 배지를 SH배지에 치상하였을 때 절편체의 약 90% 이상에서 캘러스가 형성되어 가장 높은 캘러스 형성율을 나타내었다. 유도된 캘러스의 색은 꽃색이나 배지에 따른 차이는 없었고, 아이보리나 브라운 또는 회색 계열이었다. 체세포 배발생은 WPM 기본배지에서 획득된 'Ice Wing' 꽃잎 유래 캘러스만을 분리하여3 mg/L 2,4-D와 300 mg/LL-proline을 첨가한 SH배지에 암배양 1개월 후 관찰되었다. 이후 4주 간격으로 동일한 조성의 배지로 2회 교체한 결과 체세포배가 대량 발생되는 것을 확인하였다. RT-qPCR분석에 의해'Ice Wing' 꽃잎 유래의 캘러스와 증식중인 체세포배 발생 캘러스간 캘러스로부터 체세포배 발생을 인지하는 표지로서 사용되고 있는 somatic embryogenesis receptor kinase (SERK) 유전자의 발현량을 비교한 결과, RhSERK3 유전자의 발현량은 일반 캘러스에 비해 체세포배 발생 캘러스에서 10배 높았고, 일반 캘러스에서는 거의 발현되지 않은 RhSERK4 유전자의 발현은 체세포배 발생 캘러스에서는 700배 높았다.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업(과제번호: PJ01584201)의 지원에 의해 이루어진 것임

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