• Title/Summary/Keyword: Knowledge-Based Model

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Bacterial Logic Devices Reveal Unexpected Behavior of Frameshift Suppressor tRNAs

  • Sawyer, Eric M.;Barta, Cody;Clemente, Romina;Conn, Michel;Davis, Clif;Doyle, Catherine;Gearing, Mary;Ho-Shing, Olivia;Mooney, Alyndria;Morton, Jerrad;Punjabi, Shamita;Schnoor, Ashley;Sun, Siya;Suresh, Shashank;Szczepanik, Bryce;Taylor, D. Leland;Temmink, Annie;Vernon, William;Campbell, A. Malcolm;Heyer, Laurie J.;Poet, Jeffrey L.;Eckdahl, Todd T.
    • Interdisciplinary Bio Central
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    • v.4 no.3
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    • pp.10.1-10.12
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    • 2012
  • Introduction: We investigated frameshift suppressor tRNAs previously reported to use five-base anticodon-codon interactions in order to provide a collection of frameshift suppressor tRNAs to the synthetic biology community and to develop modular frameshift suppressor logic devices for use in synthetic biology applications. Results and Discussion: We adapted eleven previously described frameshift suppressor tRNAs to the BioBrick cloning format, and built three genetic logic circuits to detect frameshift suppression. The three circuits employed three different mechanisms: direct frameshift suppression of reporter gene mutations, frameshift suppression leading to positive feedback via quorum sensing, and enzymatic amplification of frameshift suppression signals. In the course of testing frameshift suppressor logic, we uncovered unexpected behavior in the frameshift suppressor tRNAs. The results led us to posit a four-base binding hypothesis for the frameshift suppressor tRNA interactions with mRNA as an alternative to the published five-base binding model. Conclusion and Prospects: The published five-base anticodon/codon rule explained only 17 of the 58 frameshift suppression experiments we conducted. Our deduced four-base binding rule successfully explained 56 out of our 58 frameshift suppression results. In the process of applying biological knowledge about frameshift suppressor tRNAs to the engineering application of frameshift suppressor logic, we discovered new biological knowledge. This knowledge leads to a redesign of the original engineering application and encourages new ones. Our study reinforces the concept that synthetic biology is often a winding path from science to engineering and back again; scientific investigations spark engineering applications, the implementation of which suggests new scientific investigations.

Revitalization Plan and Value of Social Network Service in the Business Organization (기업조직 내 소셜 미디어 서비스 활용의 가치 제고 및 활성화 방안)

  • Kim, Dong-Hyun;Seo, Hyun-Shik;Kim, Hyung-Joon;Lee, Bong-Gyou
    • The KIPS Transactions:PartD
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    • v.18D no.4
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    • pp.275-286
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    • 2011
  • The purpose of this study is to find the beneficial plan for business success by the advantage of social media service in the business organization. There is few research for applying social media on a variety of fields in business organization although many companies are trying to find the way for its application. Hence, this study identifies the possibility for the utilization of social media services, and it also finds the plan for their effective application. The social relationship is created by increasing communications between users of social media service. The research model is established on the basis of the hypothesis that the social relationship affects knowledge share, pursuit of ego, social participation, amusement. To maximize the impact of results, the research was conducted on the basis of the target on two groups including the business men and non-business men who use social media services. As results of the research, the business men tend to appeal their impression based on sharing knowledge with anonymous people. Also, it is necessary to make a social participation to a management participation, and to utilize the social media in the organization by including amusement to its function. This research is expected to have significant implication to companies which wants to apply social media services in the future.

The Conceptual Formation of 'Gyeokchi' in the Early Joseon Period (조선 전기 '격치' 개념의 의미화)

  • Lee, Haeng-hoon
    • The Journal of Korean Philosophical History
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    • no.58
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    • pp.139-160
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    • 2018
  • 'Gyeokmulchiji' (格物致知), coming to knowledge based on the investigation of things) is a starting point for any study and politics of Confucianism. Much emphasis was placed on the conception of 'Gyeokchi' as a root of every learning and adminstration in the early Joseon period. As Confucianism established itself as a salient value system of the government, a mighty change and paradigm shift happened in its governmental system which had depended upon Buddhism up to that time. Thus, Confucian statecraft also stood out. Daehakyeonui (大學衍義) was preached as a model of regal learning and politics in the governmental agon, and its conceptual starting point was 'Gyeokchi.' The various interpretations and arguments about this concept shows the process in which Zhu Xi NeoConfucianism was deepened into Neo-Confucianism of Joseon's own. This conception reached the essence of 'Li' beyond the problem of cognitive subject and object, and provided a watershed which divided Giho (畿湖) and Yeongnam (嶺南) schools. Confucian method of study, which incorporates knowledge and practice, has great implications for our times when there are many voices of concern over humanities. The enhancement of universities and humanities is much needed to adjust the direction and pace of scientific technology, which is now entirely left with the logic of market. Accordingly, it is quite urgent for us to examine our object of learning again, which should integrate 'Sugi' (修己, cultivating oneself) with 'Chi-in' (治人, governing others), and knowledge with practice.

Case Study on the Pre-Service Earth Science Teachers' Faults Discrimination on Geological Map using Eye Tracker (시선 추적기를 활용한 지질도에서 예비 지구과학교사들의 단층 판별에 대한 사례 연구)

  • Woong Hyeon Jeon;Duk Ho Chung;Chul Min Lee
    • Journal of the Korean earth science society
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    • v.44 no.3
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    • pp.210-221
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    • 2023
  • The purpose of this study is to evaluate the content knowledge and problem solving process used by pre-service earth science teachers while discriminating faults on geological maps. For this, we collected and evaluated data on fixation duration and gaze plot, while pre-service earth science teachers (N=12) solved the problem on faults interpretation using an eye tracker (Tobii Pro Glass 2 model). The results were as follows. First, most of the pre-service earth science teachers know the concepts of the normal and reverse fault but they do not know the procedural knowledge essential for fault interpretation on geological maps. Second, the pre-service earth science teachers did not draw a geological cross-sectional map to interpret the fault on the geological map and interpreted the fault based on two-dimensional information collected from the geological map rather than three-dimensional information. Therefore, it is essential to improve the teaching and learning environment so that pre-service earth science teachers who will become earth science teachers in the future can learn procedural knowledge essential to comprehend natural phenomena including understanding natural phenomena. The results of this study can substantially help organize a new earth science curriculum or develop materials on teachers' education in the future.

A Case Study of Middle School Students' Abductive Inference during a Geological Field Excursion (야외 지질 학습에서 나타난 중학생들의 귀추적 추론 사례 연구)

  • Maeng, Seung-Ho;Park, Myeong-Sook;Lee, Jeong-A;Kim, Chan-Jong
    • Journal of The Korean Association For Science Education
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    • v.27 no.9
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    • pp.818-831
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    • 2007
  • Recognizing the importance of abductive inquiry in Earth science, some theoretical approaches that deploy abduction have been researched. And, it is necessary that the abductive inquiry in a geological field excursion as a vivid locale of Earth science inquiry should be researched. We developed a geological field trip based on the abductive learning model, and investigated students' abductive inference, thinking strategies used in those inferences, and the impact of a teacher's pedagogical intervention on students' abductive inference. Results showed that students, during the field excursion, could accomplish abductive inference about rock identification, process of different rock generation, joints generation in metamorpa?ic rocks, and terrains at the field trip area. They also used various thinking strategies in finding appropriate rules to construe the facts observed at outcrops. This means that it is significant for the enhancement of abductive reasoning skills that students experience such inquiries as scientists do. In addition, a teacher's pedagogical interventions didn't ensure the content of students' inference while they helped students perform abductive reasoning and guided their use of specific thinking strategies. Students had found reasoning rules to explain the 01: served facts from their wrong prior knowledge. Therefore, during a geological field excursion, teachers need to provide students with proper background knowledge and information in order that students can reason rues for persuasive abductive inference, and construe the geological features of the field trip area by the establishment of appropriate hypotheses.

Design and Research for Intelligent Typhoon Evasion System for Ships

  • Wang, Jing-Quan;He, Yi;Shi, Ping-An;Peng, Xiao-Hong;Xu, Zu-Yuan;Qin, Shan-Ci;Li, Qing-Lie;Ding, Bing-Lin
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2001.10a
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    • pp.177-186
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    • 2001
  • Based upon the previous experiences and typical oases of typhoon evasion fur ships as well as tile achievement in scientific research in this detrain, we developed the Intelligent Typhoon Evasion System for Ships. It consists of five subsystems, including electronic charts, ship movement management, typhoon information query and automatic plotting, real-time calculation of ship-typhoon situation, intelligent typhoon evasion decision making. With the synthetical application of analogy theory, synoptic chart, satellite cloud picture analysis, typhoon digital forecast and other relevant technologies, we leave established the typhoon evasion data bases. model bases and knowledge bases, which make it possible to automatically track the ships and typhoon paths. The system can realize ship-typhoon situation analysis, risk levee assessment, typhoon paths correction and course synoptic forecast, and intelligent typhoon evasion decision making.

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A Study on Publishing Distribution System Application for RFID System (출판유통 RFID 적용에 관한 연구)

  • 안종윤;강경식
    • Journal of the Korea Safety Management & Science
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    • v.6 no.1
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    • pp.125-133
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    • 2004
  • Greeting the globalization and information period of 21C, the improvement of the distributional system structure and modernization of publishing distribution are the seriously required periodic assignments for the publishing industry that is a national knowledge and culture industry to be equipped with the core competitiveness. First, it is to found a large distributional organization that is able to provide total logistics services in time that is essential to the publishing company and bookstore. Second, it is to have a logistics service to act up to the variable requirements of customers such as publishing companies and bookstores under the rapidly changing distribution environment. Third, it is to provide the standardized information of publishing distribution. It is needed to establish a total distributional center in collaboration to satisfy the requirements of publishing industry as above. By doing this work, structure of the publishing distribution system can be improved and its constitution can be strengthen, and it is possible not only to construct a stable supply system but also to realize an information-based system in the publishing industry with the construction of information system and its utilization. Furthermore, the application of RFID in the publishing distribution is essentially required to provide the symbolic model and logistics infrastructure into the 'Paju publishing culture information industrial complex'. It could be possible by growing the international recognition regarding distribution environment and strengthening the international competitiveness in the period of the ""Open door".

Status of ICT Convergence Smart Quality Distribution Technology for Food Quality and Safety Management

  • Kim, Jong Hoon;Kim, Ji Young;Kim, Byeong Sam
    • Agribusiness and Information Management
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    • v.6 no.2
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    • pp.13-23
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    • 2014
  • The world is in the process of a structural change related to ICT convergence knowledge industries. ICT is leading to the creation of new products and services, and is making our lives more convenient, safer, and more efficient. In advanced countries, many studies have been conducted with the goal of developing new business models converged with ICT, and this is also the case in the food industry. Korea possesses world-leading ICT, and if this ICT is applied to the food industry, a world-class new business model can be developed. The u-Food System, which is in the process of development in Korea, is a next-generation food system that can allow food providers, consumers, and distributors to access various types of information about food products, including traceability, distribution, safety, quality, and freshness, and manage this information. It is a future food system that converges ICT, biotechnology and sensing technology with food. Based on the u-Food System, this paper will introduce the status of current smart quality distribution technologies that converge ICT (such as sensor tag, sensor network, LBS, GIS, and CDMA) with food technologies (such as traceability, quality, distribution management) to manage the safety and quality of fresh food in the distribution process.

A comparison of three design tree based search algorithms for the detection of engineering parts constructed with CATIA V5 in large databases

  • Roj, Robin
    • Journal of Computational Design and Engineering
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    • v.1 no.3
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    • pp.161-172
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    • 2014
  • This paper presents three different search engines for the detection of CAD-parts in large databases. The analysis of the contained information is performed by the export of the data that is stored in the structure trees of the CAD-models. A preparation program generates one XML-file for every model, which in addition to including the data of the structure tree, also owns certain physical properties of each part. The first search engine is specializes in the discovery of standard parts, like screws or washers. The second program uses certain user input as search parameters, and therefore has the ability to perform personalized queries. The third one compares one given reference part with all parts in the database, and locates files that are identical, or similar to, the reference part. All approaches run automatically, and have the analysis of the structure tree in common. Files constructed with CATIA V5, and search engines written with Python have been used for the implementation. The paper also includes a short comparison of the advantages and disadvantages of each program, as well as a performance test.

The Methodologies of Digital Engineering Applications to Manufacturing Collaborations in Automotive Industries (자동차 산업분야의 효과적인 제조협업 구현을 위한 디지털 엔지니어링 적용 방법론에 대한 연구)

  • Lee, Yoo-Chul;Bae, Hye-Rim
    • IE interfaces
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    • v.25 no.1
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    • pp.87-95
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    • 2012
  • Very special and tentative considerations including emotional aspects are required to apply any new mechanism and methodologies for manufacturing fields due to several reasons. This study reviews the characteristics of manufacturing collaborations through specific cases applied digital engineering to enhance the collaboration performance in manufacturing domains. Two cases of collaboration related with automotive manufacturing process are analyzed to extract meaningful insights for better collaboration model suggestions. The first case deals the robot simulation to find out advance errors in jig and fixture design during the various welding process of body-in-whites. The effective communication protocol to share their idea and agreed schedules are essential for this collaboration. More severe requirement of collaboration between R&D and manufacturing departments are studied in the second case for e-coating process. The invisible barriers among different departments are lowered by the process application of Computer Aided Engineering which can make sure their own interesting effectively. Those technical and managerial suggestions can be used when the information system and standard process are sought to implement and update not only when innovation projects are executed.