• 제목/요약/키워드: Kim Jong Il

검색결과 5,056건 처리시간 0.269초

Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

  • Cho, Hee Jun;Oh, Byung Moo;Kim, Jong-Tae;Lim, Jeewon;Park, Sang Yoon;Hwang, Yo Sep;Baek, Kyoung Eun;Kim, Bo-Yeon;Choi, Inpyo;Lee, Hee Gu
    • Journal of Microbiology and Biotechnology
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    • 제29권2호
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    • pp.304-310
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    • 2019

  • Interleukin-21 is a common ${\gamma}$-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted $IFN-{\gamma}$ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

  • https://doi.org/10.4014/jmb.1811.11042 인용 PDF KSCI

1-Furan-2-yl-3-pyridin-2-yl-propenone의 TNF-${\apha}$ 유도성 MCP-1과 IL-8의 발현 억제를 통한 장 상피세포 염증 억제효과 (1-Furan-2-yl-3-Pyridine-2-yl-Propenone Inhibits TNF-${\apha}$-induced Intestinal Inflammation via Suppression of MCP-1 and IL-8 Expressions in HT-29 Human Colon Epithelial Cells)

  • 김경진;김종태;이응석;이종숙;김정애
    • 약학회지
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    • 제52권5호
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    • pp.402-406
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    • 2008

  • Previously, we have shown that 1-furan-2-yl-3-pyridin-2-yl-propenone (FPP-3) has an anti-inflammatory activity in a rat paw-edema model. In the present study, we investigated an inhibitory effect of FPP-3 on the tumor necrosis factor (TNF)-${\apha}$-induced inflammatory cytokine response in HT-29 human colon epithelial cells. Treatment with FPP-3 significantly prevented the TNF-${\apha}$-induced attachment of leukocytes to HT-29 colon epithelial cells, which is one of the pathologic hallmarks in colon inflammation. The effect of FPP-3 was markedly superior than that of 5-aminosalicylic acid (5-ASA), a commonly used drug for the treatment of inflammatory bowel disease (IBD). The pretreatment with FPP-3 inhibited TNF-${\apha}$- induced monocyte chemoattractant protein (MCP)-1, interleukin (IL)-8 mRNA expressions. In addition, FPP-3 significantly suppressed TNF-${\apha}$-induced nuclear factor (NF)-${\kappa}B$ transcription activity. These results demonstrate that FPP-3 modulates intestinal inflammation via suppressing the NF-${\kappa}B$ dependent expressions of MCP-1 and IL-8, and suggest that FPP-3 may be a valuable agent for the treatment of IBD.

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