• Journal of Dairy Science and Biotechnology
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• v.32 no.2
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• pp.131-139
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• 2014

The aim of this study was to manufacture Cutting-Gouda cheese and to investigate the change in physicochemical properties of Cutting-Gouda cheese made with Lactobacillus rhamnosus_p1. Lactic acid bacteria were isolated from Gouda cheese ripened for more than 1 year. They were identified as 2 strains of L. rhamnosus, Lactobacillus casei, Lactobacillus curvatus, and Staphylococcus saprophyticus by 16S rDNA sequencing and named L. rhamnosus_p1, L. casei_p2, L. curvatus_p3, L. rhamnosus_p4 and S. saprophyticus_p5. The proteolytic activities of isolated strains against casein were measured using prepared skim milk agar plates. L. rhamnosus_p1 showed the highest proteolytic activity. Cutting-Gouda cheese was made with L. rhamnosus_p1, and its physicochemical properties (moisture, protein, fat, ash and free amino acid content) were measured during ripening periods. Because of the modified atmosphere packaging ($N_2{^-}$), there was no change in moisture, protein, fat, and ash in the experimental group. The total amount of free amino acids in the control and experimental group gradually increased during ripening periods. The sensory evaluation showed that the experimental group was preferable to the control group. This result suggests that L. rhamnosus_p1 has potential to be developed as a new starter for Gouda cheese.

• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.64-72
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• 2021

This study aimed to identify regions with a suitable growth environment for Ligusticum chuanxing Hort. and use basic data to identify appropriate cultivation and stable production strategies. Four main areas of cultivation were selected and the relationship between growth characteristics (aboveground and underground parts) and weather and soil environment was analyzed. Overall growth was found to be significantly higher in Pyeongchang. Atmospheric and soil temperatures showed a significant negative correlation with overall height from the ground, stem diameter, and growth characteristics of the underground part; leaf length and width were positively correlated. As insolation increased, the growth characteristics, excluding leaf size, showed a positive correlation. Soil characteristics such as organic matter (OM), N, P, and K showed negative correlations with the overall height of the upper part and growth characteristics of the underground part, including stem diameter. Analysis of roots indicated that OM, N, P, and K were essential and were absorbed through the soil. The OM, N, P, and K values in the Pyeongchang area, which showed the optimal growth, were lower than those in other areas. It is believed that these results can be used to select cultivation sites for L. chuanxing and establish cultivation technology in future.

• Journal of Life Science
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• v.21 no.3
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• pp.393-399
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• 2011

The mast cell is one of the major effector cells in inflammatory reactions and can be found in most tissues throughout the body. Activated mast cells can produce histamine, as well as a wide variety of other inflammatory mediators such as eicosanoids, proteoglycans, proteases, and several pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-${\alpha}$, and interleukins (IL-6), IL-8, IL-4, IL-13. In the present study, we isolated two bacterial strains (J80 and G147) from fermented soybean and Jeotgal, and investigated the inhibitory effects of their extracts which were prepared by several pretreatment methods (sonication for 20 min, heating at $100^{\circ}C$ for 30 min, autoclaving at $121^{\circ}C$ for 15 min) on the mast cell-mediated inflammatory response. The pretreated bacterial extracts had no cytotoxicity against Human Mast Cell (HMC-1). Among various pretreatments, the extracts treated at $100^{\circ}C$ showed highest inhibition of histamine release (J80, 28.46%; G147, 41.14%). The J80 and G147 extracts treated at $100^{\circ}C$ resulted in the inhibition of IL-6 secretion by 38.46% and 56.45%, respectively. The J80 extract treated at $100^{\circ}C$ resulted in the inhibition of TNF-${\alpha}$ secretion by 66.67%, but G147 extract showed the highest inhibition effect by 41.1% when treated with sonication. These results suggest that bacterial extracts treated at $100^{\circ}C$ have a higher level of anti-inflammatory effects than other treatments such as sonication or autoclaving.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.3
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• pp.242-249
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• 1983

The experiments were carried out in farmers' field to determine the yield response of corn to fertilizers for the three years (1976-1979) The results are summarized as follow: 1. The optimum application rates of N-P-K fertilizers on corn cultivars was 21.1-11.6-10.6 kg/10a for Suweon 19, 23.0-11.9-15.0 kg/10a for Boggyo 2 and 19.8-10.6-9.1 kg/10a for Hwangok 3, and 23.3-19.1-10.7 kg/10a in drained paddy soil. 2. The optimum application rates of N-P-K fertilizers was 23.4-15.5-13.2 kg/10a in fertile soil comparing with 27.0-15.6-18.8 kg/10a in the soil of low fertility. However yield was lower in soil of low fertility than in fertile soil even under the condition applied optimum amount. 3. Production efficiencies of each kg of N, P, K application for Suweon 19, Boggyo 2, and Hwangok 3 were 26.1-24.5-9.7, 17.8-13.3-2.0, and 14.6-21.5-4.2kg. respectively. 4. The optimum nutrient content in soil and plant to yield 1,000 kg/10a of corn was available phosphorus 200 ppm, exchangeable potassium 0.63 me/100g, potassium saturation rate 5.0% in soil, and nitrogen 2.86%, phosphorus 0.73%, potassium 2.80% in plant at tasseling stage.

• Korean Journal of Environmental Agriculture
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• v.27 no.2
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• pp.105-110
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• 2008

The main purpose of this work is the development of water quality level model using the data such as DO, EC, BOD, $COD_{Cr},\;NH_3-N,\;NO_3-N,\;PO_4-P$, T-N, T-P, and SS in 88 agricultural streams of the Han river watersheds. To grant water quality level for each parameters, it divided into 20% respectively in the order of water quality level. On the basis of the lowest water quality level, water quality of streams was assigned. As the result, number of stream corresponding to Level Ⅰ was 0, Level II was 1 stream, Level III was 3 streams, Level IV was 22 streams, and Level V was 62 streams. By standardized canonical discriminant function coefficient, $NO_3-N$ was the highest in 0.427 at the discriminant power. According to discriminant function for water quality level, it was equal to $-4.648+3.246{\times}[NO_3-N],\;-5.084+3.456{\times}[NO_3-N],\;-4.298+3.067{\times}[NO_3-N],\;and\;-7.369+4.396{\times}[NO_3-N]$ from Level II to Level V, respectively. As a result of test at real data of the Han river watersheds in 2007, the suitability of water quality level model was high to 88.4%.

• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.4
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• pp.334-344
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• 2013

In order to understand the characteristics of oceanic environment in the coastal aquaculture waters of the East Sea, the observation of the CTD (temperature and salinity), dissolved oxygen, chlorophyll a and N/P (DIN ($NO_2$-N, $NO_3$-N, $NH_4$-N) : DIP($PO_4$-P)) ratio was carried out at Sokcho, Jukbyon and Gampo in February, April, June, August, October, December 2013. Based on T(temperature)-S(salinity) diagram analysis, the water masses in the study area were divided into 3 groups; Tsushima Surface Water (TSW: $20-28.3^{\circ}C$ temperatures and 31.04-33.75 salinities), Tsushima Middle Water (TMW: $8.1-16.3^{\circ}C$ and 33.00-34.49), and North Korean Cold Water (NKCW: $1.8-9.4^{\circ}C$ and 33.78-34.42). In winter, DO concentrations in the northern part were higher than those in southern part. In spring and fall, they were low in the surface layer, and increased in summer. Chl-a concentrations < $0.4{\mu}g/L$ dominated in February, April, October and December. Chl-a concentrations were higher in June and August. In particular, the highest Chl-a concentration > $2{\mu}g/L$ was observed in the middle layer of Gampo in August. In February, April, June and December, the N/P ratio in the most of the water masses was less than the Redfield ratio (16), indicating that nitrate did act as a limiting factor in phytoplankton growth. On the contrary, in August and October, the N/P ratio in surface and sub-surface layer was greater than the Redfield ratio, suggesting that phosphate was a limiting factor.

• Korean Journal of Ecology and Environment
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• v.45 no.4
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• pp.482-489
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• 2012

Visual monitoring is hard to apply on fish because they are living in a water system. To overcome this problem, acoustic telemetry, which is effective for underwater monitoring, is often used for studying fish behaviors, such as movement distance, route and patterns. In this study, in order to monitor the movement pattern of Squaliobarbus curriculus (family Cyprinidae), we used acoustic telemetry and identified the home range and movement distances. A total of nine individuals were released at two different locations: one is at the estuary barrage (Sc1~3) and the other is at the lower part of Baekjae Weir (Sc4~9), located in Geum River. Approximately, a 70 km section from the estuary barrage was investigated. Fish, which were released at the estuary barrage, utilized up to 12.7 km upstream as home range from the release site. At the lower part of Baekjae Weir, most of the fish moved and stayed within a 7.2 km downstream area, except for Sc6, which moved 53.4 km (linear maximum distance from release site) downstream from the release site. Relatively small sized fish (Sc7~9) did not show any movement. Accumulated movement distance significantly correlated with the standard length of S. curriculus ($r_s$=0.715, p=0.03). Moreover, the standard length of moving fish was significantly larger than that of not moving fish (Mann-Whitney U test, p=0.024). Therefore, the movement distance of S. curriculus has been correlated with fish size; movement distance was increased with the standard fish length. Although the sample size of monitored fish was small, various meaningful data were collected by acoustic telemetry. Consequently, this technique could be a method available for effectively monitoring the behavior and ecology of native Korean and endemic species.

• Korean Journal of Ecology and Environment
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• v.44 no.3
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• pp.264-271
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• 2011

At the Jangheung multipurpose dam, which is on the Tamjin River, a trapping and trucking operation was established to maintain continuous upstream migration of fish,. To facilitate fish gathering, installation of an effective fishing trap was required. In this study, we evaluated the fish trap, established at the Jangheung dam, using PIT (Passive Integrated Transponder) telemetry. A total of 254 individuals from 15 species were monitored. Among these tagged species, 36 individuals from 6 species (Carassius auratus, C. cuvieri, Zacco temminckii, Z. platypus, Pungtungia herzi, and Pseudobagrus koreanus) were detected; a 14.2% detection rate. C. auratus recorded the highest detection rate of 44.2% while P. herzi was 14.3%. Z. temminckii and Z. platypus showed relatively low detection, 5% and 7.7% respectively. Some of individuals from C. auratus and Z. platypus did not pass through the antenna at the first attempt but were continuously detected on multiple days. There were no statistical differences in body size (total length, standard length and body weight) of individuals that did or did not swim into the trap (Mann-Whitney U test, p>0.05). Fish mainly swam into the trap during outflow of water from the dam (Mann-Whitney U test, p<0.001) and showed a higher detection frequency in daytime than nighttime (Mann-Whitney U test, p<0.001). Thus, for fish movement into the trap, external factors such as outflow from dam and time of day have important roles. Based on detection rate, not all fishes showed upstream migration but represented selective migration. Consequently, the establishment of flexible outflow strategies that take into consideration ecological characteristics of fishes should required for improving the efficiency of fishway.

• Communications of Mathematical Education
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• v.25 no.2
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• pp.403-430
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• 2011

The purpose of the study was to investigate how the use of graphing calculators influence on forming students' mathematical concept of algebra, students' mathematical connection, and attitude toward mathematics. First, graphing calculators give instant feedback to students as they make students compare their written answers with the results, which helps students learn equations and linear inequalities for themselves. In respect of quadratic inequalities they help students to correct wrong concepts and understand fundamental concepts, and with regard to functions students can draw graphs more easily using graphing calculators, which means that the difficulty of drawing graphs can not be hindrance to student's learning functions. Moreover students could understand functions intuitively by using graphing calculators and explored math problems volunteerly. As a result, students were able to perceive faster the concepts of functions that they considered difficult and remain the concepts in their mind for a long time. Second, most of students could not think of connection among equations, equalities and functions. However, they could understand the connection among equations, equalities and functions more easily. Additionally students could focus on changing the real life into the algebraic expression by modeling without the fear of calculating, which made students relieve the burden of calculating and realize the usefulness of mathematics through the experience of solving the real-life problems. Third, we identified the change of six students' attitude through preliminary and an ex post facto attitude test. Five of six students came to have positive attitude toward mathematics, but only one student came to have negative attitude. However, all of the students showed positive attitude toward using graphing calculators in math class. That's because they could have more interest in mathematics by the strengthened and visualization of graphing calculators which helped them understand difficult algebraic concepts, which gave them a sense of achievement. Also, students could relieve the burden of calculating and have confidence. In a conclusion, using graphing calculators in algebra and function class has many advantages : formulating mathematics concepts, mathematical connection, and enhancing positive attitude toward mathematics. Therefore we need more research of the effect of using calculators, practical classroom materials, instruction models and assessment tools for graphing calculators. Lastly We need to make the classroom environment more adequate for using graphing calculators in math classes.

• Journal of Chest Surgery
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• v.30 no.2
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• pp.152-157
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• 1997

From August 1986 until June 1995, single aortic valve replacement was performed in 65 patients at the Chonnam National University Hospital. worthy-eight were male and 17 were female patients, ranging from 19 to 68 years of age(median : 43 years). The causes of the valve lesions were rheumatic in 29 patients (44.6%), bicuspid aortic valve in 6 patients (6.2%), endocarditis in 6 patients(6.2%), unknown in others. Concomitant surgical procedures were performed in 10 patients : repair of congenital defect in 5, pericardiectomy in 1, coronary artery bypass grafting in 1, noncoronary sinus plication in 1, Valsalva sinus aneurysmectomy in 1, subaortic membrane resection in 1 Used valves were 51. Jude-Medical valve in 42, Duromedics valve in 22, Bjork-Shiley valve in 2, Carpentier-Edward valve in 1. There were 3 hospital deaths (4.6%), and 2 late deaths (3.2%). Follow-up was 95.2% complete. The 10-year acturlal survival rate was 85.3%. Postoperative complications were low cardiac utput in 8, arrythmia in 5, valve related hemolysis in 1, cerebral infarction in 1, and gastrointestinal bleeding in 2. Reoperation was performed in 4 for surgical bleeding, in 3 for paravalvular leak. The mean improvement in New York Heart Association functional class is from 2.79 $\pm$ 0.66 preoperatively to 1.25 $\pm$ 0.49 postoperatively(p < 0.001) The change of cardiothoracic ratio from preoperative to postoperative is 0.57 $\pm$ 0.06 to 0.54 $\pm$ 0.05 (p < 0.05). The left ventricular ejection fraction change is not significant perioperatively. There are no mechanical failures. This early and intermediate-term follow-up suggests that in adults in whom valve repair is not possible, the mechanical valve is a reliable and durable prosthesis with good hemodynamic function and a low rate of thromboembolic event.