• International Journal of Stem Cells
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• v.16 no.1
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• pp.108-116
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• 2023

γδ T cells are a rare and unique prototype of T cells that share properties with natural killer cells in secondary lymphoid organs. Although many studies have revealed the function and importance of adult-derived γδ T cells in cancer biology and regenerative medicine, the low numbers of these cells hamper their application as therapeutic cell sources in the clinic. To solve this problem, pluripotent stem cell-derived γδ T cells are considered alternative cell sources; however, few studies have reported the generation of human pluripotent stem cell-derived γδ T cells. In the present study, we investigated whether lymphoid lineage γδ T cells were successfully generated from human pluripotent stem cells via hemogenic endothelium under defined culture conditions. Our results revealed that pluripotent stem cells successfully generated γδ T cells with an overall increase in transcriptional activity of lymphoid lineage genes and cytolytic factors, indicating the importance of the optimization of culture conditions in generating lymphoid lineage γδ T cells. We uncovered an initial step in differentiating γδ T cells that could be applied to basic and translational investigations in the field of cancer biology. Based on our result, we will develop an appropriate method to purify γδ T cells with functionality and it helpful for the study of basic mechanism of γδ T cells in pathophysiologic condition as well as clinic application.

• The Journal of the Convergence on Culture Technology
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• v.6 no.4
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• pp.813-818
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• 2020

Recently, with the rapid increase in technology and users of smart devices, the smart watch market has grown, and its utility and usability are continuously expanding. The strengths of smartwatches are wearable portability, application immediacy, data diversity and real-time capability. Despite these strengths, smartwatches have limitations such as battery limitations, display and user interface size limitations, and memory limitations. In addition, there is a need to supplement developers and standard devices, operating system standard models, and killer application modules. In particular, monitoring and application of user's biometric information is becoming a major service for smart watches. The biometric information of such a smart watch generates a large amount of data in real time. In order to advance the biometric information service, stable peer-to-peer transmission of sensing data to a remote smartphone or local server storage must be performed. We propose a synchronization method to ensure wireless remote peer-to-peer transmission stability in a smart watch system. We design a wireless peer-to-peer transmission process based on this synchronization method, analyze asynchronous transmission process and proposed synchronous transmission process, and propose a transmission efficiency method according to an increase in transmission amount.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.49 no.3
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• pp.46-55
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• 2012

Recently, the concept of PES(Personal Environment Service) is being widely discussed on various standardization organizations such as ITU-R, ETSI, 3GPP, TTA and etc. The purpose of PES is to introduce the services which can dynamically, automatically and intelligently reconfigures the electronic, electrical, and mechanical equipment surrounding the user according to the user preferences included in a user's profile by using a smartphone embedding WPAN radio technologies such as bluetooth and WiFi. This research introduces an Android Platform-based PES system which consists of a PES app, PES devices and a PES server. A smartphone platform is Android 2.2(Froyo) version and 4 simulated PES devices were implemented by using Galaxy Tab. It has shown that the PES would be a killer application of M2M(Machine-to-Machine) or D2D(Device-to-Device) in the future and it would need to study how to update a user's profile based on analyzing user's behaviour for enhancing the PES user's satisfaction.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.39 no.4
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• pp.49-59
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• 2016

Naval combat system developed in-country is progressing at an alarming rate since 2000. ROK navy will be achieved all vessels that have combat system in the near future. The importance of System Engineering and Integrated Logistics Support based on reliability analysis is increasing. However, reliability analysis that everyone trusted and recognized is not enough and applied practically for development of Defense Acquisition Program. In particular, Existing Reliability Analysis is focusing on reliability index (Mean Time Between Failure (MTBF) etc.) for policy decision of defense improvement project. Most of the weapon system acquisition process applying in the exponential distribution simply persist unreality due to memoryless property. Critical failures are more important than simple faults to ship's operator. There are no confirmed cases of reliability analysis involved with critical failure that naval ship scheduler and operator concerned sensitively. Therefore, this study is focusing on Mean Time To Critical Failure (MTTCF), reliability on specific time and Operational Readiness Float (ORF) requirements related to critical failure of Patrol Killer Guided missile (PKG) combat system that is beginning of naval combat system developed in-country. Methods of analysis is applied parametric and non-parametric statistical techniques. It is compared to the estimates and proposed applications. The result of study shows that parametric and non-parametric estimators should be applied differently depending on purpose of utilization based on test of normality. For the first time, this study is offering Reliability of ROK Naval combat system to stakeholders involved with defense improvement project. Decision makers of defense improvement project have to active support and effort in this area for improvement of System Engineering.

• Journal of Korea Spatial Information System Society
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• v.5 no.1 s.9
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• pp.13-25
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• 2003

The advent of LBS is caused by mobility advantage of wireless internet. The mobility has the information such as location which is based on LBS(Location based Services). It comes out as a killer application for wireless internet when market of wireless internet is diffused. In this paper, we build up the technical model and survey the requirement of technologies related LBS expanded as wireless internet services rapidly. First, we construct the conceptual model for LBS based on functions of LBS and characteristics of foreign models. This model is consisted of generalized components of LBS and can be used as a basic model to survey the requirement of each technologies. Second, we survey the demand of each technology for LBS. Then we analyze the importance of technologies for LBS to prepare developing various LBS. From this research, we can introduce proper plans and policy for LBS to develop technology stepwise, to promote industry activities and how to make up the foundation for LBS by government.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.12
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• pp.2221-2229
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• 2006

Optimal path search algorithm which is a killer application of mobile device to utilize location information should consider traffic flows of the roads as well as the distance between a departure and destination. The existing path search algorithms, however, are net able to cope efficiently with the change of the traffic flows. In this paper, we propose a new optimal path search algorithm. The algorithm takes the current flows into consideration in order to reduce the cost to get destination. It decomposes the road network into Fixed Grid to get variable heuristics. We also carry out the experiments with Dijkstra and Ar algorithm in terms of the execution time, the number of node accesses and the accuracy of path. The results obtained from the experimental tests show the proposed algorithm outperforms the others. The algorithm is highly expected to be useful in a advanced telematics systems.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.42 no.4
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• pp.194-202
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• 2019

Reliability analysis of the components frequently starts with the data that manufacturer provides. If enough failure data are collected from the field operations, the reliability should be recomputed and updated on the basis of the field failure data. However, when the failure time record for a component contains only a few observations, all statistical methodologies are limited. In this case, where the failure records for multiple number of identical components are available, a valid alternative is combining all the data from each component into one data set with enough sample size and utilizing the useful information in the censored data. The ROK Navy has been operating multiple Patrol Killer Guided missiles (PKGs) for several years. The Korea Multi-Function Control Console (KMFCC) is one of key components in PKG combat system. The maintenance record for the KMFCC contains less than ten failure observations and a censored datum. This paper proposes a Bayesian approach with a Dirichlet mixture model to estimate failure time density for KMFCC. Trends test for each component record indicated that null hypothesis, that failure occurrence is renewal process, is not rejected. Since the KMFCCs have been functioning under different operating environment, the failure time distribution may be a composition of a number of unknown distributions, i.e. a mixture distribution, rather than a single distribution. The Dirichlet mixture model was coded as probabilistic programming in Python using PyMC3. Then Markov Chain Monte Carlo (MCMC) sampling technique employed in PyMC3 probabilistically estimated the parameters' posterior distribution through the Dirichlet mixture model. The simulation results revealed that the mixture models provide superior fits to the combined data set over single models.

• The Journal of Society for e-Business Studies
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• v.19 no.2
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• pp.1-29
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• 2014

Advancement of mobile technology opened new business field in existing marketing area and in spirit of depression of advertisement market due to worldwide shrunken business, investment mobile advertisement market only jas a tendency to increase. In this paper I tried to know some factors to influence on the acceptance of customer to receive the referring advertisement continually through the study of 'KaKaotalk Plus Friends', a kind of marketing platform of KaKaotalk, the model killer application of Smart Phone. For this purpose I developed the research model based on Customer Value Theory and Social Exchange Theory etc. Futhermore based on these theories I studied the facts to influence on the acceptance of customer using KaKaotalk Plus Friends Service.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2007.10a
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• pp.565-569
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• 2007

To find optimal path is killer application in the telematics system. The shortest path of conventional system, however, isn't always optimal path. That is, the path with minimum travelling time could be defined as optimal path in the road networks. There are techniques and algorithms for finding optimal path. Hierarchical path algorithm categorizes road networks into major layer and minor layer so that the performance of operational time increases. The path searched is accurate as much as optimal path. At above 2 system, a method to allocate minor roads to major road region influences the performance extremely. This paper proposes methods to determine search space for selecting major roads in the hierarchical path algorithm. In addition, methods which apply the proposed methods to hierarchical route algorithm is presented.

• Journal of Broadcast Engineering
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• v.12 no.3
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• pp.203-213
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• 2007

DMB video/audio service was successfully launched. DMB data service is a matter of primary concern now. The TTI(Traffic and Travel Information) service is considered as a killer application of the data service. Tn this paper, we propose a method for design and implementation of the broadcasting system for TTI service. The proposed TTI service system consists of an authoring tool, the DB module, the Transmission module and Monitoring module. This system satisfies the national TTA standards of the Traffic and Travel Information data services for terrestrial DMB; TPEG(Transport Protocol Experts Group) platform. The system was designed to support real-time automatic transmission for CTT and CTT SUM, and non real-time authoring & automatic transmission for POI and SDI, and was focused on the capability to make high-quality contents efficiently and to send them to the data inserter reliably. The performance of the implemented system was proven through the conformance tests with the various commercial receivers. After the continuous upgrade, the system is being used in commercial service.