• Title/Summary/Keyword: Ki-66

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Study on DNA Content and Ki-67 Antibody Expression by Means of Image Analyzer for the Benign and Malignant Lesions of the Larynx (후두 편평상피의 전암성 및 악성병변에서 화상분석기를 이용한 DNA 배수성검사와 Ki-67 항체 양성세포의 분석에 관한 연구)

  • 주형로;이선희;최종욱;김인선
    • Proceedings of the KOR-BRONCHOESO Conference
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    • 1993.05a
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    • pp.89-89
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    • 1993
  • The laryngeal epithelial cell kinetics of 26 laryngeal lesions(invasive squamous cell carcinoma 14, epithelial hyperplasia 5, laryngeal nodule 7) were studied by immunehistochemical analysis with the monoclonal antibody Ki-67, which reacts with nuclear antigen in proliferating cells using paraffin embedded tissue. For DNA analysis, touch implint with fresh biopsy specimens were stained with feulgen and analyzed by image analyzer in 22 cases. 1) The proportion of Ki-67-positive cells were 32.65$\pm$ 11.59% in invasive squamous cell ca, 20.14$\pm$3.38% in epithelial hyperplasia lesion and 11.66$\pm$3.02% in laryngeal nodule. 2) DNA aneuploidy was found in 7 cases of 10(70%) invasive squamous cell carcinomas, 2 cases of 5(40%) epithelial hyperplasia lesions and all cases of laryngeal 3) Proliferation index(S phase+G2/M phase) show 23.42$\pm$11.33% in squamous cell carcinoma, 13.09$\pm$ 10.90% in epithelial hyperplasia lesion and 4.50$\pm$1.19% in laryngeal nodule. As the results, measuring the DNA content from touch imprint method together positivity of Ki-67 antibody from the microtissue during the laryngeal microscopic surgery, cell kinetics can be assessed as an effort of deciding the prognosis and provide a key to the management of precancerous lesions.

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Characteristic Comparison between PI and Hysteresis Voltage Control of High Voltage Unidirectional Inverter for Piezoelectric Load using FPGA (FPGA를 이용한 피에조 부하 구동용 고전압 단방향 인버터의 PI 및 히스테리시스 전압 제어 특성 비교)

  • Kim, Ki-Seok;Cho, Yong-Ho;Kim, Hyeong-Seop;Kang, Tae-Sam;Hong, Sun-Ki
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.66 no.1
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    • pp.48-54
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    • 2017
  • In this paper, hysteresis voltage control method is proposed to an unidirectional inverter control for piezoelectric load. Piezoelectric load has electrically RC characteristic, and is driven by the inverter to control the output voltage waveform. When controling the output waveform by PI control, appropriate gains need to be selected. However, hysteresis control may minimize the output distortion because it has maximum proportional gain. In addition, Hysteresis control algorithm has simple structure to realize and the response is fast. Although the switching frequency of the inverter by hysteresis control varies, the switching frequency for the piezoelectric load is lower than that by PI control for equivalent performance. In particular, on implementing the algorithm using FPGA, the algorithm can be implemented in fewer pabrics and the processing time can be reduced. The superiority of the proposed hysteresis voltage control was proved for piezoelectric load through simulation and experiment.

The Significance of c-Met and Ki-67 Expression in the Head and Neck Squamous Cell Carcinoma (두경부 편평세포암에서 c-Met 단백과 Ki-67 발현의 의의)

  • Kim, Jun;Do, Nam-Yong;Park, Jun-Hee;Choi, Ji-Yun;Lim, Sung-Chul
    • Korean Journal of Bronchoesophagology
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    • v.16 no.1
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    • pp.39-46
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    • 2010
  • Background and Objectives Various tumor markers have been studied in an attempt to evaluate and decide the optimal treatment of the patients with head and neek squamous cell carcinoma (HNSCC). A nuclear antigen Ki-67 is a proliferative marker of tumor cells in all phases of cell cycle except G0. c-met gene, the tyrosine kinase receptor for hepatocyte growth tactor, may play various roles in malignant transformation. The authors evaluated the prognostic significance of Ki-67 and c-Met in surgical specimens of HNSCC to determine the relationship with the various clinicopathological characteristics. Materials and Methods Formatin-fixed paraffin-embedded surgical specimens were obtained from 54 patients with HNSCC. Ki-67 and c-Met expressions were analyzed by immunohistochemical staning and were compared with the clinicopathological characteristics such as, pathologic differentiation, tumor stage, clinical stage and lymph node metastasis. Results Ki-67 and c-Met over-expression was detected in 66.7% and 90.7% in HNSCC. There was positive correlation of increased expression of Ki-67 with tumor stage. and clinical stage, increased expression of e-Met with tumor stage, clinical stage, and nodal status. The expression of c-Met had a significant positive relationship with Ki-67 index (p<0.05). Conclusion Therefore, Ki-67 and c-Met are useful markers of tumor progression, aggressiveness and prognosis in HNSCC.

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