• Molecules and Cells
• /
• v.27 no.4
• /
• pp.423-428
• /
• 2009

Superoxide dismutases (SODs) constitute the first line of cellular defense against oxidative stress in plants. SODs generally occur in three different forms with Cu/Zn, Fe, or Mn as prosthetic metals. We cloned the full-length cDNA of the Thellungiella halophila Cu/Zn-SOD gene ThCSD using degenerate RT-PCR and rapid amplification of cDNA ends (RACE). Sequence analysis indicated that the ThCSD gene (GenBank accession number EF405867) had an open reading frame of 456 bp. The deduced 152-amino acid polypeptide had a predicted molecular weight of 15.1 kDa, an estimated pI of 5.4, and a putative Cu/Zn-binding site. Recombinant ThCSD protein was expressed in Escherichia coli and assayed for SOD enzymatic activity in a native polyacrylamide gel. The SOD activity of ThCSD was inactivated by potassium cyanide and hydrogen peroxide but not by sodium azide, confirming that ThCSD is a Cu/Zn-SOD. Northern blotting demonstrated that ThCSD is expressed in roots, stems, and leaves. ThCSD mRNA levels increased by about 30-fold when plants were treated with sodium chloride (NaCl), abscisic acid (ABA), and indole-acetic acid (IAA) and by about 50-fold when treated with UVB light. These results indicate that ThCSD is involved in physiological pathways activated by a variety of environmental conditions.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.22 no.3
• /
• pp.57-68
• /
• 2017

Continuous monitoring of spatial and temporal changes in key marine environmental parameters such as SST (sea surface temperature) near IORS (Ieodo Ocean Research Station) is demanded to investigate the ocean ecosystem, climate change, and sea-air interaction processes. In this study, we aimed to develop the system for continuously measuring SST using a TIR (thermal infrared) sensor mounted at the IORS. New SST algorithm is developed to provide SST of better quality that includes automatic atmospheric correction and emissivity calculation for different oceanic conditions. Then, the TIR-based SST products were validated against in-situ water temperature measurements during May 17-26, 2015 and July 15-18, 2015 at the IORS, yielding the accuracy of 0.72-0.85 R-square, and $0.37-0.90^{\circ}C$ RMSE. This TIR-based SST observing system can be installed easily at similar Ocean Research Stations such as Sinan Gageocho and Ongjin Socheongcho, which provide a vision to be utilized as calibration site for SST remotely sensed from satellites to be launched in future.

• Korean Journal of Construction Engineering and Management
• /
• v.21 no.4
• /
• pp.62-71
• /
• 2020

Various studies on program management have been attempted due to recent changes in the environment of the construction market, and the importance of closure phase in each phase of the construction program is increasing. However, the reality is that the related research is insufficient and the field has been managed in a rule of thumb according to individual capabilities. Therefore, in order to successfully close the construction program and enhance satisfaction of stakeholders in the closure phase of the construction program, the key stakeholders were identified using the Power and Interest Matrix. The relative importance of the closure phase process was analyzed using AHP analysis method. It was also verified through on-site surveys and interviews about the proposed importance of the closure stakeholder and the relative importance of the process. It is expected that this study will increase the satisfaction of stakeholders and contribute to a successful closure if the user puts a lot of resources into the process that has a high priority in the closure phase of the construction program proposed in this study.

• Korean Journal of Remote Sensing
• /
• v.37 no.5_1
• /
• pp.1111-1124
• /
• 2021

Mudflats are crucial for understanding the ecological structure and biological function of coastal ecosystem because of its high primary production by microalgae. There have been many studies on measuring primary productivity of tidal flats for the estimation of organic carbon abundance, but it is relatively recent that optical remote sensing technique, particularly hyperspectral sensing, was used for it. This study investigates hyperspectral sensing of chlorophyll concentration on a tidal flat surface, which is a key variable in deriving primary productivity. The study site is a mudflat in Geunso bay, South Korea and field campaigns were conducted at ebb tide in April and June 2021. Hyperspectral reflectance of the mudflat surfaces was measured with two types of hyperspectral sensors; TriOS RAMSES (directionalsensor) and the Specim-IQ (camera sensor), and Normal Differenced Vegetation Index (NDVI) and Contiuum Removal Depth (CRD) were used to estimate Chl-a from the optical measurements. The validation performed against independent field measurements of Chl-a showed that both CRD and NDVI can retrieve surface Chl-a with R² around 0.7 for the Chl-a range of 0~150 mg/m² tested in this study.

• Journal of Korea Water Resources Association
• /
• v.51 no.12
• /
• pp.1207-1216
• /
• 2018

Discharge or water level predictions at tidally affected river reaches are currently still a great challenge in hydrological practices. This research aims to predict water level of the tide dominated site, Jamsu bridge in the Han River downstream. Physics-based hydrodynamic approaches are sometimes not applicable for water level prediction in such a tidal river due to uncertainty sources like rainfall forecasting data. In this study, TensorFlow deep learning framework was used to build a deep neural network based LSTM model and its applications. The LSTM model was trained based on 3 data sets having 10-min temporal resolution: Paldang dam release, Jamsu bridge water level, predicted tidal level for 6 years (2011~2016) and then predict the water level time series given the six lead times: 1, 3, 6, 9, 12, 24 hours. The optimal hyper-parameters of LSTM model were set up as follows: 6 hidden layers number, 0.01 learning rate, 3000 iterations. In addition, we changed the key parameter of LSTM model, sequence length, ranging from 1 to 6 hours to test its affect to prediction results. The LSTM model with the 1 hr sequence length led to the best performing prediction results for the all cases. In particular, it resulted in very accurate prediction: RMSE (0.065 cm) and NSE (0.99) for the 1 hr lead time prediction case. However, as the lead time became longer, the RMSE increased from 0.08 m (1 hr lead time) to 0.28 m (24 hrs lead time) and the NSE decreased from 0.99 (1 hr lead time) to 0.74 (24 hrs lead time), respectively.

• Korean Journal of Microbiology
• /
• v.55 no.2
• /
• pp.123-130
• /
• 2019

Chelatase catalyzes the insertion of divalent metal into tetrapyrrole and plays a key role in the biosynthesis of metallated tetrapyrroles, such as cobalamin, siroheme, heme, and chlorophyll. SirB is a sirohydrochlorin (SHC) chelatase that generates cobalt-SHC or iron-SHC by inserting cobalt or iron into the center of sirohydrochlorin tetrapyrrole. To provide structural insights into the metal-binding and SHC-recognition mechanisms of SirB, we determined the crystal structure of SirB from Bacillus subtilis subsp. spizizenii (bssSirB) in complex with cobalt ions. bssSirB forms a monomeric ${\alpha}/{\beta}$ structure that consists of two domains, an N-terminal domain (NTD) and a C-terminal domain (CTD). The NTD and CTD of bssSirB adopt similar structures with a four-stranded ${\beta}-sheet$ that is decorated by ${\alpha}-helices$. bssSirB presents a highly conserved cavity that is generated between the NTD and CTD and interacts with a cobalt ion on top of the cavity using two histidine residues of the NTD. Moreover, our comparative structural analysis suggests that bssSirB would accommodate an SHC molecule into the interdomain cavity. Based on these structural findings, we propose that the cavity of bssSirB functions as the active site where cobalt insertion into SHC occurs.

• Fire Science and Engineering
• /
• v.32 no.6
• /
• pp.46-54
• /
• 2018

A total of 19 elevators for evacuation were installed in the Lotte World Tower and it is planned to operate the shuttle using the manual key from five refuge floors to the 1st floor in an emergency. In the event of a fire or other disaster, it is necessary to conduct intensive analysis to determine how much RSET reduction could be achieved using the evacuation elevator compared to the existing evacuation plans. When the optimal transportation sharing ratio by the evacuation elevators was 40% at the Lotte World Tower, the RSET of the evacuation scenario in parallel with the elevators in the entire building was calculated to be 1 hour and 2 minutes. The RSET of a conventional evacuation scenario (Walking along the stairs without using the elevators) was calculated to be 1 hour 29 minutes, therefore, the former evacuation scenario were found to have a shortening effect of approximately 27 minutes compared to the latter. On the other hand, to maintain this effect, each part of the evacuation route using the elevator must have the capability to protect the evacuee from any hazards caused by fires, such as smoke, flame, and radiant heat during the evacuation. Moreover, the evacuation route should be continuous from the residence position of the elevator user to the final evacuation site, and be recognized easily.

• Korean Journal of Heritage: History & Science
• /
• v.48 no.1
• /
• pp.148-165
• /
• 2015

Iron was one of the most influential factors for formation and development of ancient countries. The diffusion of ironware had increased agricultural productivity and brought about military technical revolution. Needless to say, the rise and fall of the countries depended on the possession of stable iron production. Raw materials and fuels are the key factors for mass production of iron and a transportation route is essential to supply the goods. Jungwon area satisfies the three factors. There are many iron manufacture sites such as Jincheon Seokjang-ri Gusan-ri, and Chunju Chilgeum-dong Tangeumdae earthen ramparts in the Jungwon area. In order to study the ancient iron manufacture technique, reconstitution experiment was carried out using restored furnace which was made based on the Jincheon Seokjang-ri B-23 furnace. Some notable results were identified with the experiment as in the followings. Firstly, a roasting process has a connection with the decrease of hardness of the iron ore. Secondly, melting of the blast pipe as well as the formation of product within the furnace had a crucial effect on the cessation of the experiment. Thirdly, reduced iron in various locations within the furnace prove that there was enough reducing environment during the working. Not only melting point but also properties of iron can vary depending on the carbon contents. For the reason, formation of approximate environment in which iron can react to the chalcoal is the most important factor in terms of iron manufacture.

• Journal of the Korean Society of Mineral and Energy Resources Engineers
• /
• v.55 no.6
• /
• pp.553-563
• /
• 2018

In this study, the location conditions and optimal technologies required for creating urban municipalities that can utilize the space in an abandoned mine area, where there is no infrastructure related to recycling wastes and valuable metals, are investigated. The urban mining industry deals with mineral resources through the processing of high value-added industrial by-products and wastes, and it is a useful linkage industry for the development of mineral resources and prevention of mining hazards. Urban mining technologies targeted at the abandoned mine area constitute screening, extraction, and smelting for recycling waste products. By analyzing the technologies available, an industrial network can be developed for recycling waste batteries and catalysts, which are promising raw materials. It is also important to establish an appropriate location for related industries that can generate value-added resources, rather than the resource supply and demand conditions seen in general urban mines. In order to overcome the accessibility and infrastructure limitations, the economic foundation of the abandoned mine area should consider the linkage of raw material supply, key technologies for recycling useful mineral resources that are derived from urban mines, spatial and site conditions, and industrial characteristics.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.22 no.2
• /
• pp.558-564
• /
• 2021

The water purifier market has increased rapidly in recent years. The welding technology of the evaporator is a key component that determines the level of ice production and the cold water performance of an ice purifier. The finger type evaporator of an ice purifier can remove ice and is divided largely into an instant heat method and a hot gas method. In the hot gas type evaporator, particularly during the production process, the pinhole phenomenon inside the copper pipe and clogging problems occur intermittently when welding high-pressure pipes due to the high-temperature oxygen welding. Its use in a water purifier can cause a problem in that ice and cold water do not form, and repairs cannot be made on site. To solve this problem, in this study, a cap jig was applied to improve the welding defect of the hot gas evaporator. In addition, the oxygen welding flame size was adjusted so that the heat source could be well supplied to the cap jig, and the effectiveness was confirmed through a wave pressure test, a test, and a thermal shock test.