• Journal of Microbiology and Biotechnology
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• 제21권11호
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• pp.1166-1173
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• 2011

Meju is a traditional Korean fermented soy product used as a key element for soy sauce and doenjang. Bacilli with antimicrobial activity were isolated from meju prepared by traditional methods at Sunchang county, Jeollabukdo, Korea. Six isolates were identified as Bacillus amyloliquefaciens by recA gene sequencing and RAPD-PCR. One isolate, B. amyloliquefaciens MJ5-41, showed the strongest fibrinolytic activity. A 27 kDa active fibrinolytic enzyme, AprE5-41, was purified from the culture supernatant of MJ5-41 grown on LB by chromatographic methods. The optimum pH and temperature for purified AprE5-41 were 7.0 and $45^{\circ}C$, respectively. AprE5-41 quickly degraded $A{\alpha}$ and $B{\beta}$ chains but not the ${\gamma}$-chain of fibrinogen. AprE5-41 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide, a known substrate for ${\alpha}$-chymotrypsin, cathepsin G, and subtilisin BPN'. The structural gene, aprE5-41, was cloned by PCR and successfully expressed in B. subtilis.

• 기술혁신학회지
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• 제12권4호
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• pp.840-864
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• 2009

이른바 지방화시대에 즈음하여 지역내 기술기반 중소기업의 활발한 창업과 성공적인 경영은 지역경제발전의 주요 요소가 될 것이다. 이에 본고는 서남권지역내 기술기반기업의 창업성과요인을 분석하였다. 이를 위하여 최근의 연구문헌에 나타난 신기술기반 벤처기업 성과요인관련 메타분석결과와 서남권 지역의 특유한 정서를 토대로 연구모형을 설계하고 실증분석하였다. 분석결과에 따르면 이 지역의 신기술기반기업들의 성과요인으로서 대표자의 경영관리능력, 외부와의 기술협력 등 인적요소와 공급망관리, 기업의 정보활동 및 시장지향성 등이 높은 유의성을 갖는 상관관계 요인이었다.

• 한국산학기술학회논문지
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• 제10권7호
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• pp.1766-1772
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• 2009

본 연구에서는 한국인 아동의 우식치아로부터 S. mutans를 분리하고, acid stress하에서 분리한 S. mutans의 내산성 능력과 관련된 단백질을 규명하고자 하였다. 2D gel electrophoresis를 수행한 결과, acid stress동안 elongation factor Ts, hypothetical protein, putative amino acid ABC transporter, adenylate kinase, fructokinase, Putative 40K cell well protein precursor, peptide deformylase, shikimate 5-dehydrogenase, mannose-6-phosphate isomerase, threonine synthase, putative dTDP-glucose-4,6-dehydratase의 발현량이 뚜렷이 증가하였으며 이들 단백질은 acid stress에 관여하는 단백질들로 추정된다.

• 정보학연구
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• 제8권3호
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• pp.143-158
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• 2005

The purpose of the paper is to analyze situations and its prospect of Single Window System for custom's one-stop service in Japan and to suggest effective application was to improve of in Korea's Customs systems. In response to the growing demand for lower trading costs and shorter and more predictable lead times with the advancement of the international trade supply chain, the Single Window System for import/export and port-related procedures was launched on 23 July 2003 in Japan. The concept of the Single Window System is to establish a comprehensive computer interface system that enables users to complete all import/export and port-related procedures required under different laws and regulations in a single input and single transmission. To realize the concept, the data elements and submission times of the various systems were harmonized, and manual procedures, such as quarantine and immigration, were computerized. Then, all the necessary systems, such as NACCS, Port EDI System, and Crew Landing Permit Support System, were interconnected as a Single Window System. Close cooperation among other governmental organizations and the private sector was a key factor in the successful development and smooth utilization of the System so that it fully met all parties' needs. It is anticipated that operating costs will be greatly reduced and trade facilitation will be enhanced as a result of the simplification of procedures. Korea Customs advocates the Single Window System as a best practice at ASEAN and WCO to contribute to the development of regional and international capacity building.

• 한국빅데이터학회지
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• 제6권1호
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• pp.153-160
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• 2021

본 연구는 기업이나 기관에서 보유한 데이터의 가치 평가에 가장 많이 활용되는 소득(이익) 접근 모델의 선행 연구를 조사하고 모델의 핵심요인과 데이터 자산 가치 평가 과정의 고려사항에 대해 논의하였다. 이를 통해 데이터 자산의 공유가능성, 활용기간이 다른 기업의 자산들과 다른 특성이 있음을 규명하였다. 또한 데이터의 가치는 품질의 영향을 크게 받기에 데이터의 적시성, 정확성과 같은 다양한 관점을 검토해야하며, 사용자의 용도와 사용능력 및 가치 사슬을 총체적으로 검토하여 데이터 자산의 가치를 평가해야 함을 도출하였다. 향후 연구방향으로는 실제 현업에 적용되어야 할 모델에 대한 지속적인 연구개발 및 회계법 개정을 제언하였다.

• Journal of Korea Trade
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• 제24권5호
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• pp.35-54
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• 2020

Purpose - It seems common sense that corporate social responsibility (CSR) is a key driver of business sustainability. Nevertheless, there has been little research on the performance of socially responsible activities, including economic and environmentally responsibility activities, in internationally diversified firms. Design/methodology - The purpose of this study was to evaluate the effects of CSR activities on management performance. For this evaluation, an empirical analysis was conducted with total of 2,520 cases, selected from companies listed on the Korea Composite Stock Price Index market for six years from 2013 to 2018. As proxies for management performance, financial data such as a total asset net profit ratio and a total asset operating ratio were used. A multivariate regression analysis was conducted to test hypotheses. Findings - The results of this analysis indicated that firms in the CSR outstanding group were ranked significantly higher than other groups in management performance. In addition, CSR activities of internationally diversified firms positively influenced the total asset net profit ratio and total asset operating ratio. Originality/value - The results suggest that the CSR activities of these firms can play a significant role in enhancing management performance in the economic status of Korea, where the degree of export dependency is high.

• 한국산업정보학회논문지
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• 제23권6호
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• pp.125-138
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• 2018

최근 기업은 급속한 기술의 변화, 고객 니즈의 다변화, 경쟁 환경의 심화 등으로 인한 시장요인 뿐만 아니라 천재지변, 테러, 사회 정치적 이해관계 등의 통제 불가능한 불안요인으로 인해 예측이 어렵고 불확실성이 높은 경영 환경에 놓여 있어, 리스크 관리의 중요성이 높아지고 있다. 본 연구의 목적은 최근 들어 관심이 높아지고 있는 리스크 관리의 연구 분야에 대해 공급사슬관리(SCM) 분야를 중심으로 연구 동향을 분석하고, 해당 분야의 핵심 연구를 파악하여 해당 분야의 유용한 학문적 정보를 파악하는 것이다. Scopus 데이터베이스를 활용하여 해외 저명한 저널에 게재된 논문 정보를 수집하여 키워드 네트워크 분석과 인용 및 공저자 정보로 생성되는 네트워크를 분석하였다.

• Korean Circulation Journal
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• 제48권12호
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• pp.1135-1144
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• 2018

Background and Objectives: Mitochondria play a key role in the pathophysiology of heart failure and mitochondrial permeability transition pore (MPTP) play a critical role in cell death and a critical target for cardioprotection. The aim of this study was to evaluate the protective effects of cyclosporine A (CsA), one of MPTP blockers, and morphological changes of mitochondria and MPTP related proteins in monocrotaline (MCT) induced pulmonary arterial hypertension (PAH). Methods: Eight weeks old Sprague-Dawley rats were randomized to control, MCT (60 mg/kg) and MCT plus CsA (10 mg/kg/day) treatment groups. Four weeks later, right ventricular hypertrophy (RVH) and morphological changes of right ventricle (RV) were done. Western blot and reverse transcription polymerase chain reaction (RT-PCR) for MPTP related protein were performed. Results: In electron microscopy, CsA treatment prevented MCT-induced mitochondrial disruption of RV. RVH was significantly increased in MCT group compared to that of the controls but RVH was more increased with CsA treatment. Thickened medial wall thickness of pulmonary arteriole in PAH was not changed after CsA treatment. In western blot, caspase-3 was significantly increased in MCT group, and was attenuated in CsA treatment. There were no significant differences in voltage-dependent anion channel, adenine nucleotide translocator 1 and cyclophilin D expression in western blot and RT-PCR between the 3 groups. Conclusions: CsA reduces MCT induced RV mitochondrial damage. Although, MPTP blocking does not reverse pulmonary pathology, it may reduce RV dysfunction in PAH. The results suggest that it could serve as an adjunctive therapy to PAH treatment.

• Biomolecules & Therapeutics
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• 제27권1호
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• pp.34-40
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• 2019

Transglutaminase 2 (TGase 2) plays a key role in p53 regulation, depleting p53 tumor suppressor through autophagy in renal cell carcinoma. We found that microtubule-associated protein 1A/1B-light chain 3 (LC3), a hallmark of autophagy, were tightly associated with the level of TGase 2 in cancer cells. TGase 2 overexpression increased LC3 levels, and TGase 2 knockdown decreased LC3 levels in cancer cells. Transcript abundance of LC3 was inversely correlated with level of wild type p53. TGase 2 knockdown using siRNA, or TGase 2 inhibition using GK921 significantly reduced autophagy through reduction of LC3 transcription, which was followed by restoration of p53 levels in cancer cells. TGase 2 overexpression promoted the autophagy process by LC3 induction, which was correlated with p53 depletion in cancer cells. Rapamycin-resistant cancer cells also showed higher expression of LC3 compared to the rapamycin-sensitive cancer cells, which was tightly correlated with TGase 2 levels. TGase 2 knockdown or TGase 2 inhibition sensitized rapamycin-resistant cancer cells to drug treatment. In summary, TGase 2 induces drug resistance by potentiating autophagy through LC3 induction via p53 regulation in cancer.

• Clinical and Experimental Reproductive Medicine
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• 제46권3호
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• pp.107-111
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• 2019

Objective: Spermatogenesis is a complex process that is regulated by a number of genes, some of which are involved in folate-dependent 1-carbon metabolism. Methionine synthase (encoded by MTR) is a key enzyme participating in this pathway. This study aimed to investigate the relationship of the MTR 2756A > G polymorphism with idiopathic male fertility in the Iranian population. Methods: The participants of this study included 100 men with idiopathic infertility and 100 healthy men as the control group. Genotyping of MTR 2756A > G was performed using the polymerase chain reaction and restriction fragment length polymorphism technique. The obtained data were analyzed using SPSS ver. 20.0 with a level of confidence of p< 0.05. Results: The frequencies of the A and G alleles at this locus were 77% and 23% in infertile patients and 84% and 16% in the control group, respectively. The frequencies of the GG, GA, and AA genotypes were 5%, 36%, and 59% in the infertile patients versus 3%, 27%, and 70% in the control group, respectively. No significant difference was observed in any genetic models. Conclusion: In general, the findings of this study suggest that the MTR 2756A > G single-nucleotide polymorphism is not a predisposing factor for idiopathic infertility in men.