• Journal of Ginseng Research
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• v.47 no.5
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• pp.662-671
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• 2023

Background: 20(S)-protopanaxadiol (PPD), a ginsenoside metabolite, has prominent benefits for the central nervous system, especially in improving learning and memory. However, its transcriptional targets in brain tissue remain unknown. Methods: In this study, we first used mass spectrometry-based drug affinity responsive target stability (DARTS) to identify the potential proteins of ginsenosides and intersected them with the transcription factor library. Second, the transcription factor PURA was confirmed as a target of PPD by biolayer interferometry (BLI) and molecular docking. Next, the effect of PPD on the transcriptional levels of target genes of PURA in brain tissues was determined by qRT-PCR. Finally, bioinformatics analysis was used to analyze the potential biological features of these target proteins. Results: The results showed three overlapping transcription factors between the proteomics of DARTS and transcription factor library. BLI analysis further showed that PPD had a higher direct interaction with PURA than parent ginsenosides. Subsequently, BLI kinetic analysis, molecular docking, and mutations in key amino acids of PURA indicated that PPD specifically bound to PURA. The results of qRT-PCR showed that PPD could increase the transcription levels of PURA target genes in brain. Finally, bioinformatics analysis showed that these target proteins were involved in learning and memory function. Conclusion: The above-mentioned findings indicate that PURA is a transcription target of PPD in brain, and PPD upregulate the transcription levels of target genes related to cognitive dysfunction by binding PURA, which could provide a chemical and biological basis for the study of treating cognitive impairment by targeting PURA.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.11-22
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• 2018

Type VI secretion system (T6SS) has been discovered in a variety of gram-negative bacteria as a versatile weapon to stimulate the killing of eukaryotic cells or prokaryotic competitors. Type VI secretion effectors (T6SEs) are well known as key virulence factors for important pathogenic bacteria. In many Burkholderia species, T6SS has evolved as the most complicated secretion pathway with distinguished types to translocate diverse T6SEs, suggesting their essential roles in this genus. Here we attempted to detect and characterize T6SSs and potential T6SEs in target genomes of plant-associated and environmental Burkholderia species based on computational analyses. In total, 66 potential functional T6SS clusters were found in 30 target Burkholderia bacterial genomes, of which 33% possess three or four clusters. The core proteins in each cluster were specified and phylogenetic trees of three components (i.e., TssC, TssD, TssL) were constructed to elucidate the relationship among the identified T6SS clusters. Next, we identified 322 potential T6SEs in the target genomes based on homology searches and explored the important domains conserved in effector candidates. In addition, using the screening approach based on the profile hidden Markov model (pHMM) of T6SEs that possess markers for type VI effectors (MIX motif) (MIX T6SEs), 57 revealed proteins that were not included in training datasets were recognized as novel MIX T6SE candidates from the Burkholderia species. This approach could be useful to identify potential T6SEs from other bacterial genomes.

• Journal of the Korean Institute of Intelligent Systems
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• v.19 no.3
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• pp.355-362
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• 2009

In this paper, we present a framework for managing group behaviors in multi-agent swarm systems. The framework explores the benefits by dynamic associations with the proposed artificial potential functions to realize complex swarming behaviors. A key development is the introduction of a set of flocking by dynamic association (DA) algorithms that effectively deal with a host of swarming issues such as cooperation for fast migration to a target, flexible and agile formation, and inter-agent collision avoidance. In particular, the DA algorithms employ a so-called systematic singular association (SSA) rule for fast migration to a target and compact formation through inter-agent interaction. The resulting algorithms enjoy two important interrelated benefits. First, the SSA rule greatly reduces time-consuming for migration and satisfies low possibility that agents may be lost. Secondly, the SSA is advantageous for practical implementations, since it considers for agents even the case that a target is blocked by obstacles. Extensive simulation presents to illustrate the viability and effectiveness of the proposed framework.

• Journal of Internet Computing and Services
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• v.24 no.6
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• pp.155-160
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• 2023

In principle, data from all databases and systems managed by the Ministry of Defense or public institutions must be guaranteed to have a certain level of quality or higher, but since most information systems are built and operated, data quality management for all systems is realistically limited. Most defense data is not disclosed due to the nature of the work, and many systems are strategically developed or integrated and managed by the military depending on the need and importance of the work. In addition, many types of data that require data quality management are being accumulated and generated, such as sensor data generated from weapon systems, unstructured data, and artificial intelligence learning data. However, there is no data quality management guide for defense data and a guide for selecting quality control targets, and the selection criteria are ambiguous to select databases and systems for quality control of defense data according to the standards of the public data quality management manual. Depends on the person in charge. Therefore, this paper proposes criteria for selecting a target system for quality control of defense data, and describes the relationship between the proposed selection criteria and the selection criteria in the existing manual.

• Lingua Humanitatis
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• v.8
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• pp.331-367
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• 2006

In this paper I attempted to investigate the matters related with the clarification of the close relationship between writing system and pronunciation. On the way of pursuing the research on the subject I found the fact that the same topic has been the main academic target in Korea. There have been some remarks about English alphabets and pronunciation. Nevertheless, the relation between alphabet codes and pronunciation tokens wasn't considered as the main key to master the English pronunciation correctly and completely. As the main target of this paper I argue that it is necessary to comprehend the connection. Then, we can recognize the significant role of alphabetic structure for understanding the gist of pronunciation exercise. This paper is classified into four parts. Each part consists of the material to affirm the fact that writing system should be the inevitable equivalent of sound system, and vice versa. In the first section I show that the development of the way of pronouncing English words is closely related with the endeavors of the scholars. While performing the survey of the studies about the alphabetic structure of the age many scholars found that the spelling construction was recorded without any common denominator. Thus, they not only sought to stage the bedrock for the standard written form of words but also to associate the alphabet letters with phonetic features. Secondly I mention the negative aspect of the 'only spelling based English pronunciation education' for the educational goal of 'Phonics methodology.' In this part I suggest the essentiality of phonemic properties with the phonetic prospect: phonemic awareness. Thirdly I refer to the standardization of the spelling system of English. As the realm of application of the language is extended toward the various professional areas such as commercial, scientific, and cultural spheres, it is quite natural to assume that the usage of the language will be transformed according to the areas in the world. Fourthly I introduce the first English-Korean grammar book with the section of 'the introduction to English pronunciation.' At the chapter the author explained the sound features of English based on the regulation of 'Scientific Alphabet' of U.S.A. In the transcribing system all the symbols were postulated on the basis of the English alphabet form instead of the separate phonetic signs of IPA.

• Geophysics and Geophysical Exploration
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• v.22 no.2
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• pp.72-79
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• 2019

Underwater object detection method adopting electrical resistivity technique was proposed recently, and the need of advanced data processing algorithm development counteracting various marine environmental conditions was required. In this paper, we present an improved water tank experiment system and its operation results, which can provide efficient test and verification. The main features of the system are as follows: 1) All the processes enabling real time process for not only simultaneous gathering of object images but also the electrical field measurement and visualization are carried out at 5 Hz refresh rates. 2) Data acquisition and processing for two detection lines are performed in real time to distinguish the moving direction of a target object. 3) Playback and retest functions for the saved data are equipped. 4) Through the monitoring screen, the movement of the target object and the measurement status of two detection lines can be intuitively identified. We confirmed that the enhanced physical modeling system works properly and facilitates efficient experiments.

• 한국태양에너지학회:학술대회논문집
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• 2011.11a
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• pp.264-269
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• 2011

A precision of temperature control in the manufacturing process would be an important factor and become the main key to control production quality. Mostly manufacture machinery used oil as a coolant in their system so an accurate oil temperature control system become an absolute need in industrial field. This paper presents a experiment research to control the oil temperature constant at target point, in this experiment is $35^{\circ}C$ by using an inverter attached in compressor to varying the compressor speed. This control has been completed and tested through an experiment with different heat load of 4kW, 6kW, 7kW, 8kW and 10kW given under temperature constant room conditioned as $25^{\circ}C$. The results had shown the temperature deviation in the refrigerator has around $0.2^{\circ}C$ and the COP is 2.5 gained at 8kW and 10kW.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.21 no.3
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• pp.315-327
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• 2023

In South Korea, the exporters of items related to nuclear power generation are diversified. Consequently, there is a risk of illegitimate export by companies failing to recognize the export control system because the awareness about this system for the strategic items among the subcontractors of nuclear power facilities is limited. To prevent illegitimate export of the strategic items, it is necessary to conduct outreach activities regarding the export control system for the related companies. Additionally, the exporters and export license examiners should consider whether an export target is on the Denial List, who may divert the strategic items to weapons of mass destruction. Therefore, the Korea Institute of Nuclear Nonproliferation and Control developed two systems for controlling illegitimate export of the Trigger List items. The first system, Nuclear Industry Information Collection and Analysis System, can gather information about the key nuclear industries in Korea and analyze the dealing of strategic items. The second system, Denied Persons Information Gathering System, can regularly gather information about the denied persons and provide the updated data to the exporters and regulatory examiners. These two systems can be used for outreach activities and export license examination to prevent illegitimate export of the strategic items.

• Journal of Digital Convergence
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• v.4 no.2
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• pp.127-142
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• 2006

This paper uses a Mobilans case based on software system to derive the results of our study. Recently, the mobile phone payment entered its matured stage and became most frequently used payment method in all internet sites. And, in order to prepare for the future ubiquitous market, every mobile phone payment methods has different speed of development, market status and target segments in the market. This paper also discusses about the background and current status of Korean mobile phone payment market using SMS(Short Message Service) to purchase items on internet which is growing sharply every year. Also, we try find the key factors to succeed in this growing market. In addition to safety, delayed payment and the immediateness for use of mobile phone, we also suggest that a highly developed hardware system is an additional factor under the increased demands for the convenience with various applications.

• Journal of the Korean Society for Nondestructive Testing
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• v.32 no.6
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• pp.678-685
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• 2012

The objective of this study is to monitor the health status of harbor caissons which have potential foundation damage. To obtain the objective, the following approaches are performed. Firstly, a structural damage monitoring(SDM) method is designed for interlocked multiple-caisson structures. The SDM method utilizes the change in modal strain energy to monitor the foundation damage in a target caisson unit. Secondly, a finite element model of a caisson system which consists of three caisson units is established to verify the feasibility of the proposed method. In the finite element simulation, the caisson units are constrained each other by shear-key connections. The health status of the caisson system against various levels of foundation damage is monitored by measuring relative modal displacements between the adjacent caissons.