• BMB Reports
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• 제48권2호
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• pp.68-80
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• 2015

G protein-coupled receptors (GPCRs) are a large class of transmembrane receptors categorized into five distinct families: rhodopsin, secretin, adhesion, glutamate, and frizzled. They bind and regulate 80% of all hormones and account for 20-50% of the pharmaceuticals currently on the market. Hundreds of GPCRs integrate and coordinate the functions of individual cells, mediating signaling between various organs. GPCRs are crucial players in tumor progression, adipogenesis, and inflammation. Several studies have also confirmed their central roles in embryonic development and stem cell maintenance. Recently, GPCRs have emerged as key players in the regulation of cell survival, proliferation, migration, and self-renewal in pluripotent (PSCs) and cancer stem cells (CSCs). Our study and other reports have revealed that the expression of many GPCRs is modulated during the generation of induced PSCs (iPSCs) or CSCs as well as during CSC sphere formation. These GPCRs may have crucial roles in the regulation of self-renewal and other biological properties of iPSCs and CSCs. This review addresses the current understanding of the role of GPCRs in stem cell maintenance and somatic reprogramming to PSCs or CSCs.

• 벤처창업연구
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• 제15권5호
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• pp.261-276
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• 2020

본 연구는 성공 소상공인 창업자의 창업 이전 단계부터 성장단계까지 단계별 성공요인을 파악하여 창업 초기 인지한 성공요인과 성공 후 인지한 성공요인을 통해 효과적인 지원 방안을 모색하고자 한다. 이를 위해 성공한 편의점 가맹점주를 대상으로 창업 준비단계부터 초기, 성장단계에 따라 성공요인을 도출하기 위해 전기적 인터뷰를 통한 질적연구를 진행하였다. 소상공인 창업의 성공요인은 창업기업 성과(performance)에 대한 연구에서 적용하는 ERI모델과 ERIS모델을 활용하여 프랜차이즈 편의점 창업의 특성을 고려하여 연구했으며, 성장단계에 따른 성공요인을 도출하기 위해 프랜차이즈 성장단계를 반영한 Sasser et al.(1978)의 다점포 외식기업 수명주기(The multisite service firm life cycle)를 활용하여 성공요인을 연구하였다. 연구 결과, 성공한 가맹점의 핵심 요인은 첫 점포 입지선정, 전략적 추가 점포 입지선정, 성공적인 재계약으로 나타났다. 창업자의 특성에 따라서는 진취적 성향의 가맹점주는 전략적 추가 점포 입지선정, 상권방어 등에 적극적인 자세를 취하였고, 상대적으로 보수적인 가맹점주는 관리적 측면에서 점포운영, 재계약 등에 적극적인 자세로 경영하는 모습을 보여주었다. 특히 창업 준비단계에서는 창업가 마인드가 중요하며, 창업 전주기에서는 창업자의 경영전략의 중요성이 발견되었다. 본 연구는 이러한 연구 결과를 바탕으로 향후 성장단계에 효과적으로 적용하기 위한 창업 방안을 구체적으로 모색하였다.

• 한국산학기술학회논문지
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• 제14권4호
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• pp.1926-1934
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• 2013

M2M(Machine-to-Machine Communication)은 한 기기가 비슷한 다른 기기와 유선 혹은 무선으로 통신하는 기술로서 환경의 특성 상 저전력, 소규모, 저렴한 가격, WAN, WLAN 등 네트워크를 통하여 사물간의 통신으로 정의하고 있다. 또한 사람의 개입이 없이도 운영이 가능할 수 있는 특징이 요구된다. 그러나 사물통신 기기들의 요구사항으로 인하여 무선 통신 등에 취약점은 가지게 되고, 각 기기를 관리 혹은 제어하는 것에 대한 어려움으로 인한 취약점이 있다. 본 논문은 M2M 환경에서 Device 및 Gateway와 Network Domain간의 안전한 인증 프로토콜 기법을 제안하였다. 제안 프로토콜은 ID-Based Encryption 기반으로 인증 하며, Network Domain안에 Access Server 및 Core Server 간의 세션키를 생성한다. 그리고 생성한 세션키를 활용하여 상호간에 데이터를 송 수신하고, 키 갱신 프로토콜을 추가하여 자동으로 식별값을 갱신한다.

• 자원ㆍ환경경제연구
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• 제30권1호
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• pp.129-167
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• 2021

배출권거래제는 온실가스 감축과 저탄소 사회로의 전환을 위한 핵심 정책이다. EU 배출권거래제(EU ETS)는 운영기간, 핵심 설계 노하우 등에서 국내 배출권거래제보다 앞서 있는 좋은 벤치마크 대상이다. 이에 본 연구는 EU ETS 4기의 주요 제도 설계를 배출허용총량(Cap), 배출권 무상할당방식, 유·무상할당업종 선정 방식, 시장안정화 조치, 감축지원제도 등을 중심으로 살펴보았다. 그리고 EU ETS 1-4기의 주요 설계와 주요 설계 변경이 향후 국내 배출권거래제의 설계 및 운영에 미칠 영향을 각각 분석해보았다. 우선, 국내 배출권거래제 설계에 대한 영향으로는 벤치마크 갱신 방안 마련, 국내 산업구조 및 특성을 반영한 무상할당업종 선정 기준 마련과 2단계 평가 도입, 경매 수익의 구체적 활용방안 마련의 세 가지 요구가 증대될 것으로 예상된다. 다음으로 향후 배출권거래제 운영에 미칠 영향으로는 제도 개정의 객관적이고 심도있는 영향평가, 계획 및 제도 개정의 이른 확정을 통한 제도 안정성 및 대응 기회 제공, 배출권 거래제 거버넌스 조정 및 이해관계자 참여 확대 유도 세 가지를 들 수 있다.

• 패션비즈니스
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• 제6권1호
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• pp.137-154
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• 2002

It's not easy to renew old brand of over 50 years history to the tastes of new consumer of our time. Most of national brands that has a history of some 20 years in Korea have strove for continuation and growth of brand to no avails, which can be taken as a good example of current situation. For instance, C company, one of the National brand of US which has a history of 51 years, has made its position secure as a fashion group and based itself on a sound foundation by establishing new marketing strategy and completing successful brand renewal in the process of strategic M&A with Italian company. Those successful marketing strategies are as follows. 1) they regarded both market and consumer oriented marketing activity as company's highest priority strategy and put great emphasis upon concentration on target market and reestablishment of brand image of business casual wear. 2) Setting up and operating planning team composed of merchandizer alone in Milano, they set the direction of plan on the basis of concentrated research on potential item in market according to thorough market research done by buying office in Korea, branch office in Hong Kong and buyer in US prior to blueprint planning for season. 3) Great emphasis was placed on business which focused on intensive presentation of basic key item for apparel career women who are main consumer group in the midium-low prices market in US and on supplementation of size and color. they named this line 'collectibles' and helped their customer develop their own clothes plan without worrying about the change of color and fabric by supporting same fabric and color throughout the year and enabled them to add variation easily by supplementing new trend item. 4) Company set black as a main color that lots of apparel career women find easy to care and to express their own image and presented them with pebble which belongs to navy and beige and added fashion color such as wine and brown etc as season goes by. They constructed basic line in order for their customers to coordinate purchased item with new one or to add them to present collection, and to achieve efficient sale by setting up strategy which allows this cross coordination and changing pattern occasionally. 5) Though basic jacket for 99$, short slim skirt for 49$ are products within midium-low prices range, in the material planning stage aiming at production of item that has both resonable function appealing to consumer and is fashionable, synthetic material had to be used as a main source due to price competitiveness. Despite this situation, considering comfortable sense of fit and refined drape of silhouette that has no sign of cheap material, whole collectible line was divided into two items, which contributed to reduction of cost. In case of material that is composed of triacetate and polyester in 70 to 30 ratio, was used up to 4 million yard, which allowed drastic curtailment of cost accompanied by concentration. In case of 'collectibles' line, using Korean material mainly, C company chose to have their product sewed in Southeast Asian countries where transportation is well developed and both productivity and quality verified by operating global production system which aiming at cutdown of cost through outsourcing production from the country where labor cost is low and getting finished product. Polarization between present consumers telling us that consumers with the mind of middle classes in the past no longer exists between consumers who seek after only fine article of highest quality and wise consumers who are sensible enough to judge bubble on correlation between price and quality. To cope with this change in new consumer mind, apparel makes changing their policy so as to produce item that has reasonable quality and falls within affordable price range anywhere in the world. and they're striving to get out of difficult situation by operating global marketing strategy which stresses separation of planning, production and sale and sensibility of fashion shared worldwide. The marketing strategy of C company can be exemplified as a successful one.

• 한국경제지리학회지
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• 제20권1호
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• pp.70-83
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• 2017

경제성장에는 항상 지역간 격차가 있어왔다. 1960년대 이후 발생하기 시작한 선진 공업국의 탈 공업화로 인한 사회 경제적 위기로부터 회복하는 데에도 지역간, 도시간 상당한 격차가 있어 왔다. 이러한 격차에 대한 전통적 이론은 "자원의 불균등한 배분"으로 설명하곤 하였다. 그러나 유사한 수준의 자원을 가진 지역간에도 상당한 성장격차가 있다는 사실을 감안할 때, 그러한 전통적 이론에 문제가 있다는 것이 확인되고 있다. 이러한 상황에서 최근 성장과 회복에 있어서의 격차를 설명하는 새로운 이론이 대두되고 있다. 진화론적 경제이론에 근거한 경로이론과 회복력 개념이 그 대표적인 예이다. 이 논문은 그러한 이론의 출현 배경과 특징, 유용성 등을 비교 분석한다. 이 두 이론은 아직 객관화된 판단의 근거를 개발하지는 못했지만, 종전의 이론이 가진 한계점을 보완하고 다양한 사회현상을 설명하는 하나의 대안으로써 설득력이 있어서, 비(非)계량적 연구방법이 충분히 발달하지 못한 우리 학계의 발전에 기여할 수 있을 것으로 판단된다.

• The Korean Journal of Physiology and Pharmacology
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• 제16권4호
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• pp.265-271
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• 2012

HoxB4, a homeodomain-containing transcription factor, is involved in the expansion of hematopoietic stem cells and progenitor cells in vivo and in vitro, and plays a key role in regulating the balance between hematopoietic stem cell renewal and cell differentiation. However, the biological activity of HoxB4 in other cells has not been reported. In this study, we investigated the effect of overexpressed HoxB4 on cell survival under various conditions that induce death, using the Ba/F3 cell line. Analysis of phenotypical characteristics showed that HoxB4 overexpression in Ba/F3 cells reduced cell size, death, and proliferation rate. Moreover, the progression from early to late apoptotic stages was inhibited in Ba/F3 cells subjected to HoxB4 overexpression under removal of interleukin-3-mediated signal, leading to the induction of cell cycle arrest at the G2/M phase and attenuated cell death by Fas protein stimulation in vitro. Furthermore, apoptotic cell death induced by doxorubicin-treated G2/M phase cell-cycle arrest also decreased with HoxB4 overexpression in Ba/F3 cells. From these data, we suggest that HoxB4 may play an important role in the regulation of pro-B cell survival under various apoptotic death environments.

• 산업경영시스템학회지
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• 제42권4호
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• pp.194-202
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• 2019

Reliability analysis of the components frequently starts with the data that manufacturer provides. If enough failure data are collected from the field operations, the reliability should be recomputed and updated on the basis of the field failure data. However, when the failure time record for a component contains only a few observations, all statistical methodologies are limited. In this case, where the failure records for multiple number of identical components are available, a valid alternative is combining all the data from each component into one data set with enough sample size and utilizing the useful information in the censored data. The ROK Navy has been operating multiple Patrol Killer Guided missiles (PKGs) for several years. The Korea Multi-Function Control Console (KMFCC) is one of key components in PKG combat system. The maintenance record for the KMFCC contains less than ten failure observations and a censored datum. This paper proposes a Bayesian approach with a Dirichlet mixture model to estimate failure time density for KMFCC. Trends test for each component record indicated that null hypothesis, that failure occurrence is renewal process, is not rejected. Since the KMFCCs have been functioning under different operating environment, the failure time distribution may be a composition of a number of unknown distributions, i.e. a mixture distribution, rather than a single distribution. The Dirichlet mixture model was coded as probabilistic programming in Python using PyMC3. Then Markov Chain Monte Carlo (MCMC) sampling technique employed in PyMC3 probabilistically estimated the parameters' posterior distribution through the Dirichlet mixture model. The simulation results revealed that the mixture models provide superior fits to the combined data set over single models.

• Archives of Plastic Surgery
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• 제40권6호
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• pp.666-675
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• 2013

Background Stem cells are a unique cell population characterized by self-renewal and cellular differentiation capabilities. These characteristics, among other traits, make them an attractive option for regenerative treatments of tissues defects and for aesthetic procedures in plastic surgery. As research regarding the isolation, culture and behavior of stem cells has progressed, stem cells, particularly adult stem cells, have shown promising results in both translational and clinical applications. Methods The purpose of this review is to evaluate the applications of stem cells in the plastic surgery literature, with particular focus on the advances and limitations of current stem cell therapies. Different key areas amenable to stem cell therapy are addressed in the literature review; these include regeneration of soft tissue, bone, cartilage, and peripheral nerves, as well as wound healing and skin aging. Results The reviewed studies demonstrate promising results, with favorable outcomes and minimal complications in the cited cases. In particular, adipose tissue derived stem cell (ADSC) transplants appear to provide effective treatment options for bony and soft tissue defects, and non-healing wounds. ADSCs have also been shown to be useful in aesthetic surgery. Conclusions Further studies involving both the basic and clinical science aspects of stem cell therapies are warranted. In particular, the mechanism of action of stem cells, their interactions with the surrounding microenvironment and their long-term fate require further elucidation. Larger randomized trials are also necessary to demonstrate the continued safety of transplanted stem cells as well as the efficacy of cellular therapies in comparison to the current standards of care.

• 동의생리병리학회지
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• 제34권4호
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• pp.177-183
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• 2020

The Hippo-YAP signaling pathway is critical for cell proliferation, survival, and self-renewal in both Drosophila and mammals. Disorder of Hippo-YAP pathway leads to tumor development, progression and poor prognosis in various cancers. YAP/TAZ are the key downstream effectors of the Hippo pathway and they can be inhibited through LATS1/2, core kinases in the Hippo pathway, mediated phosphorylation. In this study, we investigated the effect of Angelica gigas Nakai extract (AGNE) on Hippo-YAP/TAZ pathway. First, ANGE induced YAP/TAZ phosphorylation and dissociation of the YAP/TAZ-TEAD transcription complex. By qRT-PCR, we found that ANGE inhibits the expression of YAP/TAZ-TEAD target gene, CTGF and CYR61. In addition, the transcriptional activity of YAP/TAZ was not suppressed significantly in LATS1/2 double-knockout (DKO) cells by ANGE compared to LATS1/2 wild-type (WT) cells, which means AGNE inhibits YAP/TAZ signaling through direct action on LATS1/2. Further, it was confirmed that AGNE-induced activation of LATS1/2 inhibited the migration potential of the vector-expressing cells by suppressing YAP/TAZ activity. The reduced migration potential was restored in active YAP-TEAD expressing cells. Taken together, the results of this study indicate that ANGE downregulates YAP/TAZ signaling in cells through the activation of LATS1/2.