• 한국멀티미디어학회논문지
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• 제11권8호
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• pp.1101-1110
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• 2008

네스팟(Nespot)은 편리한 무선 인터넷 접속 서비스를 제공한다. 초기 접속 과정에서 사용자 아이디 및 패스워드 정보를 입력받아 IEEE 802.1X EAP-MD5 기반 인증 단계를 수행한다. 하지만 네스팟에서 사용되는 EAP-MD5 기반 사용자 인증 단계에서 송수신되는 패킷을 수집하여 분석한 결과 사용자 ID 값이 노출되어 전송되고 있으며, 네스팟 서버와 접속 장치간 상호 인증의 취약성이 발견되었다. 이에 본 연구에서는 네스팟에서 사용하는 IEEE 802.1X EAP-MD5 기반 사용자 인증 시스템의 취약점을 분석하고, 추가적으로 간략화된 일회용 세션키(one-time session key) 공유 방식을 적용하여 상호 인증 및 무선 구간의 암호화 기능을 제공하는 등 기존 네스팟 공중 무선랜 기반 사용자 인증 서비스에서의 취약점을 보완하고 이를 개선할 수 있는 기법을 제시하였다.

• Journal of Microbiology
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• 제44권4호
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• pp.369-374
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• 2006

To investigate the mechanism of salt tolerance of gram-positive moderately halophilic bacteria, two-dimensional gel electrophoresis (2-D PAGE) was employed to achieve high resolution maps of proteins of Halobacillus dabanensis $D-8^T$. Approximately 700 spots of proteins were identified from these 2-D PAGE maps. The majority of these proteins had molecular weights between 17.5 and 66 kDa, and most of them were distributed between the isoelectric points (pI) 4.0 and 5.9. Some protein spots were distributed in the more acidic region of the 2-D gel (pI <4.0). This pattern indicated that a number of proteins in the strain $D-8^T$ are acidic. To understand the adaptation mechanisms of moderately halophilic bacteria in response to sudden environmental changes, differential protein profiles of this strain were investigated by 2-D PAGE and $Imagemaster^{TM}$ 2D Platinum software after the cells were subjected to salt shock of 1 to 25% salinity for 5 and 50 min. Analysis showed 59 proteins with an altered level of expression as the result of the exposure to salt shock. Eighteen proteins had increased expression, S proteins were induced, and the expression of 33 proteins was down-regulated. Eight of the up-regulated proteins were identified using MALDI-TOF/MS and MASCOT, and were similar to proteins involved in signal transduction, proteins participating in energy metabolism pathways and proteins involved in stress.

• 스마트미디어저널
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• 제7권4호
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• pp.52-60
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• 2018

마이크로그리드(MG:Microgrid)의 에너지 관리 시스템(EMS:Energy Management System)은 마이크로그리드 내 기기로부터 데이터를 수집, 분석하여 운영자를 비롯하여 사용자 및 타 시스템에 정보를 제공한다. 이 과정에서 유무선 통신망을 통하여 안전하게 정보를 제공하기 위한 방법이 필요하다. 본 논문에서는 마이크로그리드 내의 장치로부터 데이터를 수집하는 RTU(Remote Terminal Unit)와 EMS간의 데이터 전송 과정에서 발생 가능한 데이터의 노출 및 변조로부터 안전한 시스템을 개발하기 위하여 암/복호화, 키 관리 및 키 분배, 메시지 인증 기능을 제공하는 모듈을 설계, 구현하였다. RTU에 대해 하나의 VD(Virtual Device)를 연결하여 통신함으로써 RTU에 대한 연결 관리 및 키 관리의 효율성을 증대할 수 있다.

• 융합정보논문지
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• 제9권10호
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• pp.9-15
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• 2019

최근 차세대 전자상거래 및 금융 분야에서는 비트코인, 이더리움 등의 블록체인 기반 기술에 관심이 크다. 블록체인 기술의 보안성은 안전하다고 알려졌지만, 가상화폐 관련 해킹 사건/사고들이 이슈화되고 있다. 가상화폐 지갑에 대한 로그인 세션 탈취, 악성코드 감염으로 인한 개인키 노출, 단순한 암호 사용 등 외부환경의 취약성이 주요 원인이었다. 그러나 개인키 관리는 전용 애플리케이션 활용 또는 로컬 백업, 문서 프린트를 통한 물리적 보관 등 일반적인 방법을 권장하고 있다. 본 연구에서는 화이트박스 암호 기반 개인키 보호 기법을 제안한다. 안전성 및 성능분석 결과 개인키 노출 취약점에 대한 안전성을 강화하고, 암호화키를 알고리즘에 내장하여 기존 프로토콜의 처리 효율성을 증명하였다.

• Journal of Periodontal and Implant Science
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• 제30권3호
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• pp.675-687
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• 2000

Multiple periodontal pathogens sequentially colonize the subgingival niche during the conversion from gingivitis to destructive periodontal disease. An animal model of sequential immunization with key periodontal pathogens has been developed to determine whether T and B lymppocyte effector functions are skewed and fail to protect the host from pathogenic challenge. The present study was performed to evaluate immunomodulatory effect of exposure to Fusobacterium nucleatum(F. nucleatum) prior to Porphyromonas gingivalis(P. gingi - valis). Group 1(control) mice were immunized with phosphate-buffered saline, Group 2 were immunized with F. nucleatum prior to P. gingivalis, while Group 3 were immunized P. gingivalis alone. All the T cell clones derived from Group 2 demonstrated type 2 helper T cell clone(Th2 subsets), while those from Group 3 mice demonstrated Th1 subsets. Exposure of mice to F . nucleatum prior to P. gingivalis interfered with opsonophagocytosis function of sera against P. gingivalis. In adoptive T cell transfer experiments, in vivo protective capacity type 2 helper T cell clones(Th2) from Group 2 was significantly lower than type 1 helper T cell clones(Th1) from Group 3 against the lethal dose infection of P. gingivalis. Western blot analysis indicated the different pattern of recognition of P .gingivalis fimbrial proteins between sera from Group 2 and Group 3. In conclusion, these study suggest that colonization of the subgingival niche by F .nucleatum prior to the periodontal pathogen, P. gingivalis, modulates the host immune responses to P. gingivalis at humoral, cellular and molecular levels.

• 한국식품위생안전성학회지
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• 제23권1호
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• pp.80-84
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• 2008

유해물질의 식품기준은 위해성이 인정될 때 정하는 것을 원칙으로 한다. 위해평가의 절차에 거쳐 정량적 수치로 인정되는 위해성은 정부의 관리방향을 설정하는데 중요한 정보를 제공한다. 대표성 있는 모니터링자료가 확보되어야 정확한 위해평가를 수행할 수 있으므로 만성적 건강영향을 나타내는 유해물질의 경우 장기적 모니터링결과를 확보하는 것이 필요하다. 과거에는 대다수 인구집단의 안전에 초점을 두었으나, 최근에는 고섭취에 의한 유해물질 고노출그룹, 동일노출 수준에서도 유해영향을 크게 나타내는 민감그룹, 노약자, 임산부 등의 안전까지도 검토 후 소수그룹까지 안전할 수 있는 기준을 설정하는 추세이다. 유해물질 기준검토를 위하여 최신의 독성정보, 통계정보, 분석기술정보, 낮출 수 있는 한 낮출 수 있는 ALARA(as low as reasonably achievable)정보들이 필요하다.

• 한국환경보건학회지
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• 제41권4호
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• pp.223-230
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• 2015

Objectives: There is considerable evidence that polluted ambient air contributes to the risk of cardiovascular morbidity and mortality. Heart rate variability (HRV) is defined as the variation in heartbeat intervals and has been reported as a biological marker of cardiovascular disease. This article reviews the existing literature in order to examine the association between air pollution and HRV. Methods: Literature was searched using Web of Science with the key words of "air pollution", "heart rate variability" and other related terms. A total of 156 articles were listed. For review, 21 of those listed publications were chosen after excluding studies regarding chamber studies, occupational environment, secondhand smoke and automobile exhaust. Results: Research methods employed in the publications were classified by type of participants (elderly/adult), air pollution monitoring (ambient/personal) and HRV monitoring (continuous/spot). Among HRV parameters, power in the low frequency range (LF), power in the high frequency range (HF) and standard deviation of all NN intervals (SDNN) were all associated with air pollutants. The chosen studies were mostly based on elderly populations. In studies based on continuous HRV monitoring, LF and SDNN significantly decreased when $PM_{2.5}$ exposure increased. Conclusion: Continuous HRV monitoring combined with personal exposure monitoring has been one of the most common study methods in recent publications. We expect that this review will be useful for the study of the association between air pollution and cardiovascular effects using HRV.

• 약학회지
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• 제41권6호
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• pp.789-796
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• 1997

The purpose of this study was to determine the effect of sulfinpyrazone on fumonisin $B_1$-induced elevation of free sphingoid bases in LLC-$PK_1$ cells. Fumonis ins are a family of mycotoxins produced by the fungi Fusarium moniliforme which is common contaminant in corn. Fumonisins are also potent inhibiors of sphingosine and sphinganine N-acyltransferases (ceramide synthases), key enzymes in sphingolipid metabolism resulting in the elevation of free sphinganine. The cytosolic concentration of fumonisin B1 was known to be closely proportional to the elevation of free sphinganine in LLC-PK1 cells [Yoo, H.-S., Norred, W.P., Wang, E., Merrill, A.H., Jr., and Riley, R.T. (1992) Toxicol. Appl.Pharmacol. 114. 9-15]. Sulfinpyrazone, an anion transport inhibitor, reduced the elevated level of free sphinganine resulting from fumonisin B1 inhibition of de novo sphingolipid biosynthesis. Fumonisin B1 at a concentration of 20${\mu}$M showed approximately 120pmol/$10^6$ cells relative to 3-10pmol/$10^6$ cells in control cultures, and sulfinpyrazone at a concentration of 200${\mu}$M partially reversed the increased level of free sphinganine induced by fumonisin $B_1$ down to normal level after exposure to fumonisin $B_1$ for 8 to 24hr. However, the reduced effect of sulfinpyrazone on the fumonisin $B_1$-induced elevation of intracellular sphinganine was not shown after 24hr. Fumonisin $B_1$ exposure to LLC-PK1 cells for 36 and 48hr showed approximately 74 and 80pmol per $10^6$ cells relative to 82 and 76pmol,respectively, in fumonisin $B_1$ plus sulfinpyrazone-treated cultures. Sulfinpyrazone-induced less elevation of free sphinganine in confluent cells after exposure to fumonisin $B_1$ suggested that either sulfinpyrazone may block the availability of fumonisin $B_1$ to cells or act on the fumonisin $B_1$ interaction with ceramide synthase.

• 대한소아치과학회지
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• 제32권3호
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• pp.543-549
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• 2005

상악 견치는 가장 긴 치근을 가지며 저작기능과 함께 하악의 측방운동을 유도하고 심미적으로도 치열궁의 구각부위에 위치하여 교합관계의 조화와 대칭을 유지하는 등의 중요한 기능을 수행한다. 상악 견치는 발육기간이 가장 길며 형성되어 맹출하기까지 복잡한 경로를 갖기 때문에 다른 치아보다 매복의 빈도가 높다. 이러한 매복치를 치료하기 위해 임상의는 관찰을 하거나 외과적 노출과 교정적 견인, 자가치아이식, 발치 등 다양한 치료방법을 고려할 수 있다. 외과적 노출과 교정치료는 견치를 교합선상에 배치할 수 있기 때문에 가장 이상적인 접근으로 생각할 수 있다. 본 증례 보고는 구개측으로 매복된 상악 견치를 외과적 노출과 교정적 치아이동을 통해 기능적, 심미적으로 양호한 결과를 얻었다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권11호
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• pp.1789-1800
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• 2019

Objective: Although alveolar macrophages play a key role in the respiratory immunity of livestock, studies on the mechanism of differentiation and survival of alveolar macrophages are lacking. Therefore, we undertook to investigate changes in the lipid metabolism and survival rate, using 3D4/31 macrophages and Dudleya brittonii which has been used as a traditional asthma treatment. Methods: 3D4/31 macrophages were used as the in vitro porcine alveolar macrophages model. The cells were activated by exposure to phorbol 12-myristate 13-acetate (PMA). Dudleya brittonii extraction was performed with distilled water. For evaluating the cell survival rate, we performed the water-soluble tetrazolium salt cell viability assay and growth curve analysis. To confirm cell death, cell cycle and intracellular reactive oxygen species (ROS) levels were measured using flow cytometric analysis by applying fluorescence dye dichlorofluorescein diacetate and propidium iodide. Furthermore, we also evaluated cellular lipid accumulation with oil red O staining, and fatty acid synthesis related genes expression levels using quantitative polymerase chain reaction (qPCR) with SYBR green dye. Glycolysis, fatty acid oxidation, and tricarboxylic acid (TCA) cycle related gene expression levels were measured using qPCR after exposure to Dudleya brittonii extract (DB) for 12 h. Results: The ROS production and cell death were induced by PMA treatment, and exposure to DB reduced the PMA induced downregulation of cell survival. The PMA and DB treatments upregulated the lipid accumulation, with corresponding increase in the acetyl-CoA carboxylase alpha, fatty acid synthase mRNA expressions. DB-PMA co-treatment reduced the glycolysis genes expression, but increased the expressions of fatty acid oxidation and TCA cycle genes. Conclusion: This study provides new insights and directions for further research relating to the immunity of porcine respiratory system, by employing a model based on alveolar macrophages and natural materials.