• Journal of Microbiology and Biotechnology
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• v.23 no.4
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• pp.451-458
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• 2013

The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.

• Physical Therapy Korea
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• v.8 no.2
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• pp.73-85
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• 2001

The purpose of this study was to investigate whether the standing balance could be influenced by the different foot positions. Seventeen patients with hemiplegia were tested for the static and dynamic balance under the different foot positions. In the balance test, subject stood by bearing weight on one foot, and the other foot was positioned in three different positions (symmetric, $45^{\circ}$ anterolateral, and anterior position). This study used the Kinesthetic ability trainer (KAT2000) to measure the standing balance. The results were as follows: 1) There were significant differences in the static standing balance in different foot positions with both weight-bearing on the paretic limb and on the nonparetic limb (p<.05). 2) There were also significant differences in the dynamic standing balance in different foot positions with both weight-bearing on the paretic limb and on the nonparetic limb (p<.05). 3) There was a significant difference when the paretic weight-bearing and the nonparetic weight-bearing were compared (p<.01). 4) when the paretic weight-bearing and the nonparetic weight-bearing were compared, anterior foot position showed a significant difference in the dynamic standing balance (p<.05), but $45^{\circ}$ anterolateral foot position did not show a significant difference (p>.05). In this study, the standing balance showed a significant difference according to different foot positions in hemiparetic patients, and standing balance was better when they stood by bearing weight on the nonparetic limb. These results indicate that it is a necessary to consider both weight-bearing limb and foot position not only in the rehabilitation program but also in achieving the stability in the independent life.

• The Korean Journal of Zoology
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• v.39 no.1
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• pp.36-46
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• 1996

An endogenous phenoloxidase (EPO) from earthworm, Lumbricus rubellus, has been purified and characterized. The purified EPO using ammonium sulfate fractionation, Blue-2, Phenyl-, and Q-sepharose chromatography steps was revealed in SDS-PAGE as a single protein banri with Mr. of 59 kl)a. A native strudure of the enzyme was examined with an in situ staining of a nondenatudng-PAGE using DL-dopa as a substrate. The result showed that a single band due to the EPO activity was located siighdy above a standard polypeptide with Mr. of 210 kl)a. These fads indicate that the EPO is an oligomeric enzyme. The presence of a monophenolase activity of the purified EPO, which hydroxylates tyrosine to dopa, was confirmed by observing dopachrome accumulation at 475 nm at PH 8.0 with a typical lag phase during 60 mm. of meausrement. A series of inhibition study has been performed for the enzyme with several divalent cation chelators such as phenyithiourea (Flu), 1, lO-phenanthroline, EDTA, and EGTA. Among them, only V'flj inhibited the enzyme with 1C0.5 of 65 MM, which indicated that copper was critical for the catalysis of EPO. The enzyme was maximally active at 35'C and pH 8.0 when L-dopa to dopachrome conversion was spectrophotometricaily monitored at 475 nm. The apparent Km values of P0 for L-opa were obtained as 1.86 mM and 13.8 mM at pH 6.5 and 8.0, respectively. The catalytic efficiencies at both pH were almost identical [(kat/Km)pH8.0/(kcat/Km)pH6.5 = O.92] while the Vmax at p11 8.0 was 6.6-fold higher than that at pH 6.5. This fact may indicate that pH affeds the catalysis at substrate and/or enzyme-substrate complex level rather than the enzyme itself. Taken together, the EPO was an oligomeric enzyme which did not require proteolysis for its activation. These results also indicated that the enzyme can exist, at least, in part as a latent form In vivo, which might be distinct from the prophenoloxidase activating system. Therefore, it is pertinent to consider that there must be certain regulatory molecules or phenomena in L. rubellus which make the 1,0 in a latent form in vivo before the foreign invasions.

• Korean Institute of Interior Design Journal
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• v.22 no.5
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• pp.225-232
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• 2013

The spatial separation of male's and female's area due to the law of 'Naeoei' is one of the trademark features of residence by the building principle of 'Confucianism' in the upper class house in the late of the Chosun Dynasty. Thanks to modernized agriculture and commerce, the residence of 'Sinheung-Yangban' developed. And they were influenced by the economic and living characteristics than traditional custom. So the residence of 'Sinheung-Yangban' is very important in terms of the course of modernizing of the korean traditional residence. Researcher figures out the influences of the law of 'Naeoei' in residence and the characteristics of male's and female's field in terms of daily life with investigating the residence of 'Sinheung-Yangban' as the upper class house in the late of the Chosun Dynasty. Through this, the goal of this study is to find the properties of male's and female's field of upper class houses in the late of the Chosun Dynasty. With this study, the followings are the characteristics of male's and female's spatial composition of upper class houses in Gyong-gi Province in the late of the Chosun Dynasty. First, the traditional law of 'Naeoei' plays a primary role as the principle of building of upper class houses in Gyong-gi Province in the late of the Chosun Dynasty. With a natural result, there is a clear spatial separation as the male's and female's social role. Second, 'An-Chae' and 'Sarang-Chae' as male's and female's living space are separated physically, but real living life tends to be syntagmatically made with 'An-Madang' as the center. Third, spatial integration of 'An-Madang' as the center results in the development of architectural connection factors of 'An-Chae' and 'BaKat-Chae' adjoining 'An-Madang', facing 'Toei-Gan', 'Maru', and 'door'. Forth, the boundary of male's and female's areas is relatively clear between dwellers and visitors, but on the other hand, the separation of male's and female's spaces between family members has little significance.

• Journal of Fashion Business
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• v.12 no.3
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• pp.87-98
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• 2008

The purposes of this study are to measure physiological reactions of human body according to the scalp treatment, a popularized service in beauty care industry and propose efficient ways of scalp treatment. To meet the goals, total 30 applicants without any medical history(5 males and 5 females in 20's, 30's and 40's respectively) were informed on the purpose of experiment hereof and were investigated and received a 30-minute scalp treatment, which combines standardized scalp treatment massage technique proposed by KAT and ITF with another massage technique operated in the beauty salon run by the author of this paper. 5ml of blood samples were taken from each subject before and after the scalp treatment respectively and the blood sample was divided into 3 different tubes for analysis: 1) 2 ml for blood cell analysis, 2) 2ml for enzyme activity measurement, 3) 1ml for hormone level reading. In order to determine effects of scalp treatment on ALP, GOT, GPT, ${\gamma}-GTP$, WBC, RBC, Hb, Hct, Platelet, MCV, MCH and MCHC, all collected data were used for measuring respective levels of these blood substances by means of enzyme reaction measurement, enzyme activity measurement and automated hematology analyzer. Then, all measured data were analyzed through paired t-test using SPSS WIN 11.5. As a result, the scalp treatment is associated with improving hepatic function, facilitating blood circulation and helping blood coagulation and hemostasis in a effective way. Therefore, it would be necessary to conduct further studies on this subject related to anemia in the future.

• Journal of Korean Physical Therapy Science
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• v.10 no.2
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• pp.123-130
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• 2003

The purpose of this study is to examine whether whole-body balance taping therapy could contribute to take balance of all of the body and to decrease the pain. Data were collected from 20 people who received the treatments of physical therapy in one welfare center of Daegu city for four weeks from 6. Jan. 2003 to 7. Feb. 2003. A simple questionnaire and VAS for evaluation of a pain degree was used. And the Biomyth 7 was used to measure the whole-body balance and the sample exercise protocol for KAT 2000, which is a balance training device tool, was also used as an evaluation tool. The result of the study showed that balance level of human body was improved after receiving the whole-body balance taping therapy. Total, Right Left, Front, Back score of the balance level before the therapy was 1033.6, 656.1, 377.6, 639.9, 394.5 respectively. However, the scores of them after the therapy was decreased to 432.0(p<0.01), 293.2(p<0.01), 142.3, 287.7(p<0.05), 143.9(p<0.05) respectively. And the degree of pain before the therapy in the Back and Knee was 6.1, 6.7 respectively. However, the degree of the pain after the therapy was decreased to 3.1(p<0.01), 3.1(p<0.01) respectively. Further studies using case - control groups are required because this study only deal with the before-after test for the same group.

• Physical Therapy Korea
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• v.4 no.3
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• pp.34-44
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• 1997

The purpose of this study was to compare the balance ability at different foot angle with KAT 2000 (Breg, Inc., Vista, CA. 1994). Forty-nine (male 24, female 25) normal subjects participated in this study. All subjects were assessed under two conditions. One was eye-opened condition and the other one was eye-closed one in 3 psi surface condition. All subjects were tested at different foot angle that were toe-in $25^{\circ}$, $45^{\circ}$, toe-out $25^{\circ}$ and $45^{\circ}$. The subject attempted to keep the platform as stable as possible with eyes closed and with eyes opened for every 20 seconds. The starting position was that subject crossed their arms across chest and flexed knees slightly. The results of each test were showed by a score on screen, which meant balance index. The se collected data were analyzed by using oneway ANOVA, Scheffe test, and t-test. The results of this study were as follows: 1. When the foot angle were changed, balance index was the lowest in toes-out $25^{\circ}$ condition and greatest with toes-in $45^{\circ}$ with eyes opened and with eyes closed. There were statistically significant difference with eyes opened and with eyes closed (p<0.05). 2. There was statistically significant difference in balance index according to visual condition and the balance index tested with eyes closed was higher than with eyes closed(p<0.05). 3. There was statistically significant difference in balance index by gender(p<0.05).

• The Research Journal of the Costume Culture
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• v.9 no.1
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• pp.61-72
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• 2001

Chunghahk-dong is located in a retired spot, Jiri Mountain and has been built up its won religion and culture without any exchange outside world. People in Chunghahk-dong believe in a religion called Genjungyutaosim. Forming a trinity on which mind is Taoism, body is Buddhism, and behavior is confucianism. These are the principle elements to form a village. They made their own unique cultural factor such as longhaired boys and girls, wearing traditional cloths and hat, a monastic life, folk mores and family rituals, and private school system. This shows a great deal of affection on their life style and organization and is closely connected with community and culture. Grasping a culture and behavior on clothing, it has propose to understand Chunghahk-dong properly according to this study. A method of study has a purpose to understand culture and clothing behavior precisely in Chunghahk-dong. I inquire ito clothing behavior with investigation and study of picture script. The results are as followed; People in Chunghahk-dong show unique clothing and hair style behavior based on Genjungyutao. First, men and women wear a white cloth called HanBok(Korean costume) and footwear made of rubber. Only men wear a blue vest. A grown up men wear a long coat when they go out. They are dressed in tractional cloth for a ceremonial occasion. The reason why they put on the HanBok(Korean costume) is a belief on our traditional cloth becomes a standard dress in the future. They believe Korea becomes a standard dress in the future. They believe Korea becomes a standard dress in the future. They believe Korea become a leading country in the world. This clothing behavior is symbol of Genjungyutao and has a role of delivery system for expressing their split. Second, In sign of being a Genjungyutao men, they let their grow hair. Cutting hair is not to be a Genjungyutao men any more. There is few reason that people in Chunghahk-dong let grow their hair : First, because of importance in TanGun's ideology, they believe Korea is a first nation in the world and can't cut their hair which has grown from TanGun (The founding father of the Koran nation) Chosun traditionally. Second, Cutting their hair runs counter to the principle of nature. Third, They value their body for confucianism. They don't want to damage their body because of body from their parents. Boys and girls braid and tie up their hair and adult tie a topknot and a chignon. Wearing a YouGun(A horsehair skullcap) at home and Kat(A Korean top hat) when going out, they express as a Genjungyutao. Hemp cloth and synthetic fibers are main clothes. Also, they wear an improved HanBok(Korean costume) these days on influence outside.

• Physical Therapy Korea
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• v.3 no.3
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• pp.1-11
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• 1996

The purpose of this study were to evaluate and compare the balance ability at different conditions in normal 20 to 29 years old on unstable platform, KAT 2000(Breg, Inc., Vista, CA. 1994). Static and dynamic BI(balance index) were measured 3psi and 5psi surface conditions. Static tests were done on right and left leg separately, then both legs together with the feet apart 20cm with the eyes opened and closed. Dynamic tests were done on both legs together with apart 20cm with the eyes opened. A dynamic test was performed in which the subject moved platform in a circular manner to chase a moving object on a computer screen. Seventy healthy students(average 21.6 years, male, female) were tested. In this study applied the paired t-test and correlation to determine the statistical significance of result. The results were as follow: 1) The mean static balance index of the Rt leg was $119.9{\pm}75.72$ on 3psi surface condition with the eyes opened, and that of the Lt leg was $224.3{\pm}121.16$. 2) The mean static balance index of the Rt leg was $93.1{\pm}24.16$ on 5psi surface condition with the eyes opened, and that of the Lt leg was $180.5{\pm}61.76$. 3) The mean static and dynamic balance index of both legs were $76.4{\pm}31.86$, $2187.6{\pm}696.99$ on 3psi with the eyes opened, and $68.3{\pm}14.82$, $1938.7{\pm}525.41$ on 5psi respectively. 4) The mean static balance index of the Rt leg was $517.8{\pm}220.87$ on 3psi surface condition with the eyes closed, and that of the Lt leg was $588.6{\pm}204.81$. 5) The mean static balance index of the Rt leg was $271.9{\pm}192.151$ on 5psi surface condition with the eyes closed, and that of the Lt leg was $363.4{\pm}98.97$. 6) The mean static balance index of both legs was $332.6{\pm}137.31$ on 3psi surface condition with the eyes closed, and that of the 5psi was $288.5{\pm}133.07$. 7) The balance index on 3psi surface condition was significantly higher than that of 5psi (p<0.05, p<0.01). 8) The balance index with the eyes closed was significantly higher than that of the eyes opened (p<0.05). 9) The balance index on the left leg was significantly higher than that of the right leg (p<0.05, p<0.01). 10) There was no correlation between static balance index and dynamic balance index. 11) Therewas no correlation between weight or height and balance index.

• The Journal of Korean Physical Therapy
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• v.16 no.4
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• pp.90-114
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• 2004

This study was conducted to examine the influence and effect of muscle enforcement program on Activity of daily living(ADL) improvement and posture balance of the old, and to provide more effective muscle enforcement program and educational data. The muscle enforcement exercise program was performed on the old(institution, 16 men, 10 women) for 8 weeks from April 22, 2002 through June 17,2002. Programed Exercise 1 - Exercise 10 were practised 8 times per program for 3 days a week. The load of exercise was increased per two weeks. The methods of measurement were questionnaire, Indiana 47903(action-response analysis machine) and Sample exercise protocol for KAT 2000(balance training device). SAS/PC statistic analysis was used for data analysis. T-test was used for analysis of change before and after exercise in this study. The summary and conclusions are as follows. 1. On subjectively recognized health states, the healthy were $42.3\%$. On the satisfaction with health states, the satisfied were $50.0\%$. On the factors of effects on daily-life behavior performance, the group who had troubles was $50\%$ and the group who was so and so was $34.6\%$ compared with the old of the same age. On prospect about health states in the future, the group who would be better was $38.\%$. On effective methods for problem solving, exercise was $42.3\%.\;88.5\%$ of respondents answered the need of health care. The participation intention in health program was $92.3\%$. 2. On the change of psychological emotion and behavior aspects, the group who had repeated complaints or anxieties and reduced activities or interests was effective(P<0.01). 3. On the improvement effects of IADL difficulties, the group who had difficulties in doing daily-life indoors was improved effectively compared with before and after exercise(P<0.01). On medication management, the effects of improvement after exercise were high compared with before exercise(P<0.01), the effects of improvement was high on the whole. 4. On the effects of ADL function improvement, putting on upper clothing and lower clothing was improved effectively(P<0.05), toilet use and individual sanitation was improved effectively(P<0.05). 5. On the effects of action-response, the results of 8weeks regular exercise program were not different significantly compared with before and after exercise. The behavior quickness of the old by muscle enforcement program was not increased. This means that the old needs much time for exercise sense training because of the regression of cognition sense. 6. In the effect of posture balance, the whole grades were effective from 1272.69 before excercise to 476.92 after exercise(P<0.01). Especially right balance 657.65 was lowered to 208.57 after exercise most effectively(P<0.01). Rear balance 776.34 before exercise was lowered to 136.65 after exercise. The results of measurement were significant(P<0.05).