• Title/Summary/Keyword: Karp

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A RADIOGRAPHIC STUDY OF MENTAL FORAMEN IN INTRAORAL RADIOGRAPHS (구내방사선사진상의 이공에 관한 연구)

  • Sohn Jeong-Ick;Choi Karp-Shik
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.25 no.2
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    • pp.501-511
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    • 1995
  • The purpose of this study was to evaluate the position and shape of mental foramen in periapical radiographs. For this study, periapical radiographs of premolar areas were obtained from the 200 adults. Accordingly, the positional and shape changes of mental foramen were evaluated. The authors obtained radiographs according to changes in radiation beam direction in periapical radiographs of premolar areas, and then evaluated the positional and shape changes of mental foramen. The following results were obtained: 1. Shapes of mental foramen were observed elliptical(34.3%), round or oval(28.0%), unidentified(25.5%) and diffuse(12.2%) type in descending order of frequency. 2. Horizontal positions of mental foramen were most frequently observed at the 2nd premolar area(55.3%), the area between the 1st premolar and 2nd premolar(39.6%), the area between the 2nd premolar and 1st molar(3.4%), the 1st premolar area(1.0%), the area between the canine and 1st premolar(0.7%) in descending order of frequency. 3. Vertical positions of mental foramen were most frequently observed at the inferior to apex(67.1%), and at apex(24.8%), overlap with apex(6.4%), superior to apex(1.7%) in descending order of frequency. 4. Shapes of mental foramen were more obviously observed at the upward 10° positioned periapical radiographs. And according to the changes of horizontal and vertical position, they were observed similar to normally positioned periapical radiographs.

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Myelination and Demyelination of Schwann cells and Neuron cells (슈반세포와 뉴런세포의 수초화와 탈수초화)

  • Kim, Hyun Joo;Kim, Ji-Young;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.830-833
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    • 2015
  • Schwann cells and neuron cells from dorsal root ganglion (DRG) of rat embryos (E16) were isolated and purified in vitro system. The purified DRG cells with anti-mitotic agents and purified Schwann cells, respectively, were cocultured and then consummated myelination processing. This myelination system was treated by M. leprae-specific phenolic glycolipid-1 (PGL-1) and then accomplished demyelination system. We compared with myelination and demyelination using neurofilament of monoclonal antibody.

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A Novel Possibility of Recombinant Baculovirus Vector (재조합 베큘로바이러스 벡터의 새로운 가능성)

  • Kim, Ji-Young;Kim, Hyun Joo;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.10a
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    • pp.838-841
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    • 2015
  • Recombinant baculovirus vector is composed of genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). This recombinant baculovirus vector was transfected into cell lines and tissues and then found out a novel possibility in view of gene transfer and gene expression in comparison to other vector systems. Efficacy of gene transfer and gene expression of this recombinant baculovirus vector was higher than any other vector system.

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Building an e-TRM System to Increase the Efficiency of National R&D Planning (효율적 국가 R&D 기획을 위한 e-TRM 시스템 구축)

  • Kim, Karp-Soo;Park, Eui-Suk;Lee, Sung-Joo;Lim, Byung-Hyuck
    • IE interfaces
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    • v.23 no.4
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    • pp.264-274
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    • 2010
  • The potential of roadmapping systems in updating, maintaining and sharing technology roadmaps has been argued in many articles but the systems have not been dealt with as a research topic. Therefore, this research purposes to develop an online roadmapping system, which is called an e-TRM system, to improve the efficiency of roadmapping at the national level in Korea. For the purpose, the roadmapping process for national R&D planning and its main activities were analyzed, based on which system requirements and main functions were identified. After the functions were successfully implemented in the TRM system, the system was applied to the 2009 national roadmapping process to verify its feasibility and utility. The system will enable to digitalize R&D planning in Korea and share the planning results nationwide. Also, the research results are expected to provide useful guidelines for those who are in charge of developing TRM systems.

Construction and Transfection of Recombinant Baculovirus Vectors (재조합 베큘로바이러스 벡터의 제조와 감염)

  • Sa, Young Hee;Lee, Ki Hwan;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.05a
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    • pp.700-703
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    • 2017
  • Baculovirus vectors were recombined using uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These novel recombinant vectors were infected into various cell lines. We performed and analyzed gene transfer and gene expression of these recombinant vectors comparison with other control vectors. From this result, we identified that these recombinant vectors have higher efficient gene transfer and expression of than control vector.

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Baculovirus Vector Reconstructed with Useful Genes (유용한 유전자들로 재구성된 베큘로바이러스 벡터)

  • Kim, Ji-Young;Kim, Hyun Joo;Sa, Young-Hee;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.05a
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    • pp.711-714
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    • 2016
  • Recombinant baculovirus was reconstructed with useful genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). This reconstructed vector was infected into various cell lines and tissues. We investigated gene transfer and gene expression of this reconstructed vector in comparison to other vectors and recognized that this reconstructed vector was higher effective than any other control vector.

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Infection of Semliki Forest Virus Induces Demyelination of Neuron (Semliki Forest Virus 감염은 뉴런의 탈수초를 유발한다)

  • Kim, Hyun Joo;Sa, Young-Hee;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.05a
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    • pp.714-717
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    • 2017
  • Schwann cells and Neuronal cells were isolated from dorsal root ganglion (DRG) in embryos of rat in vitro respectively. The cultured Schwann cells and cultured neuronal cells, respectively were co-cultured in a same plate. These cells were performed accomplishment of myelination. This myelinated co-culture system was infected by Semliki forest virus and then induced demyelination processing in this myelinated co-culture. We identified myelination and demyelination processing using antibody of peripheral myelin protein 22 (PMP 22) meaning presence of myelinated neuron.

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Demyelination of Neuron by Infection of Semliki Forest Virus (Semliki forest virus 감염에 의한 뉴우런의 탈수초)

  • Kim, Hyun Joo;Kim, Ji-Young;Sa, Young-Hee;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.05a
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    • pp.718-721
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    • 2016
  • Schwann cells and neuron cells from dorsal root ganglion (DRG) in embryos of rat were cultured in vitro respectively. The purified neronal cells added with anti-mitotic agents and purified Schwann cells were cocultured and then accomplished myelination processing. Infection of Semliki forest virus into this myelinated co-culture system was performed and then accomplished demyelination. We identified myelination and demyelination processing using antibody of neuropeptide Y meaning presence of myelinated neuron.

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Isolation of Schwann Cell and Separation of Schwann Cell-Neuron Network from Mouse Embryo (마우스 배아에서 슈반세포-뉴런 네트워크의 분리와 슈반세포의 분리)

  • Kweon, Tae-Dong;Sa, Young-Hee;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.10a
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    • pp.943-945
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    • 2013
  • The study of Schwann cell myelination has been facilitated by the availability to isolate and establish pure population of primary Schwann cells. Dorsal root ganglia (DRG) of mouse embryo as source of Schwann cells were used in this study. This method includes three steps: first step of dissociation of the embryonic DRG, second step of expansion of Schwann cell precursors, followed by mechanical separation of the Schwann cell-neuronal network from the underlying fibroblasts, and third step of purification of Schwann cells from the associated neurons and subsequent expansion of the purified Schwann cells. We made a highly purified population of Schwann cells and Schwann cell-neuron networks in a short period using this procedure.

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Binding Geometry of Inclusion Complex as a Determinant Factor for Aqueous Solubility of the Flavonoid/β-Cyclodextrin Complexes Based on Molecular Dynamics Simulations

  • Choi, Young-Jin;Lee, Jong-Hyun;Cho, Kum-Won;Hwang, Sun-Tae;Jeong, Karp-Joo;Jung, Seun-Ho
    • Bulletin of the Korean Chemical Society
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    • v.26 no.8
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    • pp.1203-1208
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    • 2005
  • A computational study based on molecular dynamics (MD) simulations was performed in order to explain the difference in aqueous solubilities of two flavonoid/$\beta$-cyclodextrin ($\beta$-CD) complexes, hesperetin/$\beta$-CD and naringenin/$\beta$-CD. The aqueous solubility of each flavonoid/$\beta$-CD complex could be characterized by complexwater interaction not by flavonoid-CD interaction. The radial distribution of water around each inclusion complex elucidated the difference of an experimentally observed solubility of each flavonoid/$\beta$-CD complex. The analyzed results suggested that a bulky hydrophobic moiety (-$OCH_3$) of B-ring of hesperetin nearby primary rim of $\beta$-CD was responsible for lower aqueous solubility of the hesperetin/$\beta$-CD complex.