Proceedings of the Korean Institute of Information and Commucation Sciences Conference (한국정보통신학회:학술대회논문집)

The Korea Institute of Information and Commucation Engineering (한국정보통신학회)
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Myelination and Demyelination of Schwann cells and Neuron cells

슈반세포와 뉴런세포의 수초화와 탈수초화

  • Kim, Hyun Joo (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Kim, Ji-Young (Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine) ;
  • Hong, Seong-Karp (Division of Bio and Health Sciences, Mokwon University)
  • Published : 2015.10.26
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Abstract

Schwann cells and neuron cells from dorsal root ganglion (DRG) of rat embryos (E16) were isolated and purified in vitro system. The purified DRG cells with anti-mitotic agents and purified Schwann cells, respectively, were cocultured and then consummated myelination processing. This myelination system was treated by M. leprae-specific phenolic glycolipid-1 (PGL-1) and then accomplished demyelination system. We compared with myelination and demyelination using neurofilament of monoclonal antibody.

쥐의 배아(배아 16일)의 척수신경절에서 슈반세포와 뉴런 세포가 실험실 상에서 분리되었고 정제되었다. 정제된 척수신경절 세포는 유사분열 억제제를 첨가하여 배양되었고 분리 정제된 슈반세포와 공동배양 배양시켰다. 이 결과로 마침내 수초화 과정이 구축되었다. 이 수초화는 M. leprae-specific phenolic glycolipid-1 (PGL-1) 성분으로 처리하였고 그 결과 neurofilament의 단클론 항체를 이용하여 수초화와 탈수초화를 비교 및 분석하였다.

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