• Korean Journal of Medicinal Crop Science
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• v.26 no.6
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• pp.455-463
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• 2018

Background: Sancho (Zanthoxylum schinifolium Siebold and Zucc) oil is used as a traditional medicinal material to treat severs stomach inflammation and as a diuretic. This study was carried out to investigate the effect of addition of antioxidants and blended oil the storage stability and safety of the biomaterial. Methods and Results: The effects of temperature and light on sancho oil were investigated, and the ability of antioxidants in preventing rancidity of the oil was discovered. Under fluorescent light and in darkness, the acidity of the oil was much lower than that under direct sunlight. The addition of antioxidants decreased the acid value of sancho oil; the antioxidant that showed the best results in this regard was 0.5% propolis. The acid value of canola oil, which had the lowest acid value compared with that of other oils, and blended oil, containing 5% canola oil in sancho oil, decreased by 5.5% and 15%, respectively. About one acid value decrease was observed for every 1% increase in blending with canola oil. As the concentration of canola oil increased, the viscosity and the elightness (L valu) of sancho oil increased slightly, while the blueness (b value) decreased. Conclusions: The results of this study may contribute to ensuring food safety during preservation and the industrialization of the presevation of sancho oil.

• Journal of Life Science
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• v.25 no.10
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• pp.1169-1175
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• 2015

Although many studies have described the physiological effects of bee products, such as honey, propolis, pollen, and royal jelly, the health benefits of honeycomb remain incompletely characterized. We performed a comparative study of the antioxidant properties of honey and honeycomb extracts using two different solvents (water and 95% ethanol). The results showed that the total phenolic and flavonoid content of the honeycomb extract was higher than that of the honey extract. They also demonstrated that water was more effective than ethanol in extracting total phenols. The in vitro antioxidant properties of the water honeycomb extract were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO^•) radical scavenging assays and ferrous ion chelating and reducing power assays. The antioxidant activity of the honeycomb extract exhibited was higher than that of the honey extract. The 50% effective concentrations (EC₅₀) of the honeycomb extract were 7.3±0.26 mg/ml for scavenging DPPH radicals, 6.1±0.22 mg/ml for scavenging NO^• radicals, 6.9±0.44 mg/ml for chelating ferrous ions, and 8.2±0.11 mg/ml for reducing power. A correlation analysis revealed that the total phenolics and flavonoids of the honeycomb extract were the major contributors to the radical scavenging activity, ferrous ion chelating, and reducing power. The honeycomb extract was effective in protecting biological systems against various oxidative stresses in vitro. This is the first report on the antioxidant properties of honeycomb.

• Current Research on Agriculture and Life Sciences
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• v.7
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• pp.99-107
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• 1989

The study was conducted to obtain some basic information to establish the system of performance-tests and selection of honeybee queens(Apis mellifera) under Korean circumstances, Colony performances were tested with thirty colonies of Apis mellifera at two apiaries in Taegu, Korea from September, 1988 to August 1989. The results of performance-testing on the colonies are summarized as follows : The colony weight measured before wintering was averaged $23.6{\pm}1.90kg$ and the colony weight was decreased by $2.9{\pm}0.82kg$ in average during winter season. Thirteen colonies were entered in two story hive from thirty single box colonies from April 17 to May 5, 1989 with increase of bee population and, consequently, the ability of enter-supers of the colonies apperared to be low. The ability of collecting pollen was measured to be $14.8{\pm}2.15gr$ per colony during 24 hours in April, and the number of swarm cells was counted $12.5{\pm}3.43$ cells per colony in aveage. Tendency to use propolis appeared to be moderate, and the number of returning foragers for a minute per colony was counted $108.7{\pm}18.31$ bees in average. Brood area was measured $2,464{\pm}628,67cm^2$ per colony in the post nectar flow season of acasia, and 30.8 percent of the colonies appeared to be infected with chalkbrood disease, The amount of honey production was $14.9{\pm}8.49kg$ per colony, which was harvested two times during the main nectar flow season of acasia.

• Journal of Life Science
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• v.28 no.1
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• pp.37-42
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• 2018

Non-steroidal anti-inflammatory drug-activated gene-1 (NAG-1) is a transforming growth factor beta (TGF-${\beta}$) superfamily gene associated with pro-apoptotic and anti-tumorigenic activities. In the present study, we investigated if caffeic acid phenethyl ester (CAPE) derived from propolis could induce the expression of anti-tumorigenic gene NAG-1. Our results indicate that CAPE significantly induced NAG-1 expression in a time- and concentration-dependent manner in HCT116 cells. We also found that CAPE induced NAG-1 expression in a concentration-dependent manner in another human colorectal cancer cell line, LOVO. In addition, CAPE triggered apoptosis, which was detected with Western blot analysis using poly-(ADP-ribose) polymerase antibody. NAG-1 induction by CAPE was not dependent on transcription factor p53, which was confirmed with Western blot analysis using p53 null HCT116 cells. The luciferase assay results indicated that the new cis-elements candidates were located between -474 and -1,086 of the NAG-1 gene promoter. CAPE dramatically induced activating transcription factor 3 (ATF3) expression, but not cAMP response element-binding protein (CREB), which shares the same binding sites with ATF3. The co-transfection experiment with pCG-ATF3 and pCREB showed that only ATF3 was associated with NAG-1 up-regulation by CAPE, whereas CREB had no effect. In conclusion, the results suggest that CAPE could induce the expression of anti-tumorigenic gene NAG-1 mainly through ATF3.

• The Plant Pathology Journal
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• v.23 no.3
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• pp.174-179
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• 2007

Seedlot disinfection techniques to control mung bean sprout rot caused by Colletoricum acutatum and C. gloeosporioides were evaluated for commercial production scheme. Soaking seedlots in propolis (100 X) and ethanol (20% for 30 min) appeared promising with control values of 85.5 and 80.8 respectively, but still resulted in up to 20% rot incidence. None of the C. acutatum conidia survived through hot water immersion treatment (HWT) for 10 min at temperatures of 55, 60 and $65^{\circ}C$, whereas the effective range of the dry heat treatment (DHT) was $60-65^{\circ}C$. Tolerance of mung bean seedlot, as estimated by hypocotyl elongation and root growth, was lower for HWT than for DHT. Germination and growth of sprouts were excellent over the range of $55-65^{\circ}C\;at\;5^{\circ}C$ intervals, except for HWT at $65^{\circ}C$ for 5 min. At this marginal condition, heat damage appeared so that approximately 2% of seeds failed to sprout to normal germling and retarded sprouts were less than 5% with coarse wrinkled hypocotyls. These results suggested that DHT would be more feasible to disinfect mung bean seedlots for commercial sprout production. Heat treatment at above ranges was highly effective in eliminating the epiphytic bacterial strains associated with marketed sprout rot samples. HWT of seedlot at 55 and $60^{\circ}C$ for 5 min resulted in successful control of mung bean sprout rot incidence with marketable sprout quality. DHT at 60 and $65^{\circ}C$ for 30 min also gave good results through the small-scale sprouting system. Therefore, we optimized DHT scheme at 60 and $65^{\circ}C$ for 30 min, considering the practical value of seedlot disinfection with high precision and accuracy. This was further proved to be a feasible and reliable method against anthracnose incidence and those bacterial strains associated with marketed sprout rot samples as well, through factory scale mung bean sprout production system.

• Korean Journal of Microbiology
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• v.40 no.4
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• pp.334-341
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• 2004

Polysaccharides were prepared from Orostachys japonicus by extration with hot steam water (OJPl). The OIPl fraction was further purified by Sephadex G-50 gel filtration chromatography to produce FI (polysaccharides) and FII (oligosaccharides) fraction. The average molecular masses o fFI and FII fraction were determined to be 3050 kDa and 13 kDa, respectively. The antimicrobial activity of OIPl was tested against 8 strains of bacteria and one strain of yeast by the disc diffusion method, fluorescein diacetate (FDA) method and broth dilution method. The OIPl exhibited a very strong growth inhibition to Candida albicans. The OIPl remarkably sup­pressed the growth of Salmonella typhimurium and Staphylococcus aureus. The OIPl showed higher growth inhibition to Escherichia coli and Pseudomonas aeruginosa than propolis, positive control. When the anticancer activity of the OIPl, FI or FII was examined against human cancer cell lines and the Sarcoma 180 cells, these widely suppressed the proliferation of cell lines in the MTT assay and morphology study. Especially, they remarkably inhibited the growth of A549, HeLa and AGS cells. Also treatment of cancer cells with OJPl, FI or FII induced apoptotic cell death characterized by DNA fragmentation. The OJPl, FI or FII exhibiting various biological activities such as antimicrobial activity and anticancer activity is expected to be developed as new biohealth products.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.539-545
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• 2022

The aim of this study was to evaluate the effect of beekeeping by-products added to drinking water on the growth performance and intestinal and fecal microflora of Institute of Cancer Research (ICR) mice. A total of 72 five-week-old ICR mice with an initial body weight (BW) of 24.57 ± 0.60 g were used in a two-week experiment. The four treatment groups were as follows; 1) CON, normal distilled water; 2) T1, CON with 0.7% beehive extract; 3) T2, CON with 0.7% propolis (PRO); and 4) T3, CON with 0.7% royal jelly (RJ). Each treatment consisted of 6 replicate cages with 3 mice per cage. At 0 - 1 week, T3 showed a significantly higher (p < 0.05) body weight gain (BWG) and feed efficiency (G : F) than that of CON. Compared with CON, T2 showed a significantly higher (p < 0.05) BWG and feed intake at 1 - 2 weeks. During the entire period, T2 and T3 showed a significantly higher (p < 0.05) BWG and G : F compared to CON. The amount of Salmonella and Lactobacillus in the large intestine was significantly decreased and increased (p < 0.05) in T2 and T3, respectively, compared to CON. The amount of Escherichia coli in the fecal matter was significantly reduced (p < 0.05) compared to CON in all treatment groups to which beekeeping by-products were added. In conclusion, the addition of PRO or RJ to the drinking water of ICR mice had a positive effect on the growth performance and the intestinal and fecal microflora.

• Journal of Nutrition and Health
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• v.57 no.5
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• pp.469-480
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• 2024

Purpose: Sarcopenia is defined as the age-related loss of muscle mass and strength. Although the etiology, pathogenesis, and diagnosis of sarcopenia are not completely understood, diabetes has been implicated in the development of frailty and functional impairment associated with sarcopenia. This study examined the relationship between diabetes and sarcopenia and explored the efficacy of chrysin, a natural flavonoid found in honey and propolis, as a preventive agent for diabetic sarcopenia. Methods: A mouse myoblast cell line (C2C12) was incubated in media containing 5.5 mM glucose or 33 mM glucose (high glucose) with or without 20 µM chrysin for up to seven days. In addition, a type 2 diabetic animal model, db/db mice, was administered chrysin (10 mg/kg/day) orally for 10 weeks. The protein levels were quantified using western blot analysis with antibodies targeting specific myogenic transcription factors, such as MyoD, myogenin, and Myf5, as well as proteins associated with muscle protein degradation, including Murf1, Atrogin1, and myostatin. Results: Hyperglycemia downregulated the expression of myogenic transcription factors (MyoD, myogenin, and Myf5) and proteins that regulate muscle degradation (Murf1 and Atrogin1) in C2C12 cells treated with high glucose and db/db mice. The chrysin treatment counteracted these effects, promoting the expression of these factors. These results suggest that chrysin can regulate muscle protein turnover and prevent muscle atrophy in diabetic sarcopenia. Conclusion: Chrysin has potential as a bioactive ingredient in preventing diabetic sarcopenia.

• Korean Journal of Poultry Science
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• v.34 no.1
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• pp.43-52
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• 2007

Experiments were conducted to investigate the effects of dietary botanicals (herbs and plant extracts) on the performance, nutrient metabolizability, small intestinal microflora, IgG level and blood parameters in broiler chickens. In Exp. 1, 1,000 (500 each sex) broiler chicks($Ross^{(R)}$) were divided into 20 groups of 50 chickens each(25 birds each sex). Four groups were assigned to each of five dietary treatments:control and diets containing antibiotics($Avillamix^{(R)}$, avillamycin-premix), Herb M(Herb $mix^{(R)}$), Plant extract B(BIOSTRONG $510^{(R)}$) and Plant extract A($APEX^{(R)}$). In Exp. 2, 240(120 each sex) broiler chicks($Ross^{(R)}$) were devided into six treatment groups:control and diets containing antibiotics($Avillamix^{(R)}$, avillamycin-premix), Plant extract D($Digestarom^{(R)}$), Plant extract P($Phellozyme^{(R)}$), Plant extract G($Galicin^{(R)}$) and Plant extract C(CRINA $POULTRY^{(R)}$). Each treatment consisted of four replicates of 10 birds each. In both experiments, birds had free access to diets and water for 5 wk on floor pens(Exp. 1) and cages(Exp. 2). In Exp.1, production index of groups fed diets supplemented with herbs and plant extracts was slightly higher than the control and those fed Herb M was highest. In Exp. 2, groups fed diets supplemented with herbs and plant extracts consumed more feed than the control during the period between 4 and 5 wk(P<0.05). Feed conversion(feed/gain) was lower in antibiotics group than other groups. The values of RBC, Hb and HCT were higher(P<0.05) in chicken fed diets supplemented with the additives than in the control in Exp. 1. BA value was lower(P<0.05) in groups fed diets supplemented with the additives than in the control in Exp. 2. Serum IgG were higher(P<0.05) in groups fed diets supplemented with the additives than in the control in both experiments. The cfu of intestinal microflora and metabolizability of nutrients were not significantly different among treatments in both experiments. It was concluded that the botanical supplements can be used as an alternative to antibiotics in broiler diets.