The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Effective Heat Treatment Techniques for Control of Mung Bean Sprout Rot, Incorporable into Commercial Mass Production

  • Lee, Jung-Han (Department of Applied Biology and Environmental Sciences, Gyeongsang National University) ;
  • Han, Ki-Soo (Department of Applied Biology and Environmental Sciences, Gyeongsang National University) ;
  • Kim, Tae-Hyoung (Department of Applied Biology and Environmental Sciences, Gyeongsang National University) ;
  • Bae, Dong-Won (Central Laboratory, Gyeongsang National University) ;
  • Kim, Dong-Kil (Department of Applied Biology and Environmental Sciences, Gyeongsang National University) ;
  • Kang, Jin-Ho (Research Institute of Life Science, Gyeongsang National University) ;
  • Kim, Hee-Kyu (Department of Applied Biology and Environmental Sciences, Gyeongsang National University)
  • Published : 2007.09.30
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Abstract

Seedlot disinfection techniques to control mung bean sprout rot caused by Colletoricum acutatum and C. gloeosporioides were evaluated for commercial production scheme. Soaking seedlots in propolis (100 X) and ethanol (20% for 30 min) appeared promising with control values of 85.5 and 80.8 respectively, but still resulted in up to 20% rot incidence. None of the C. acutatum conidia survived through hot water immersion treatment (HWT) for 10 min at temperatures of 55, 60 and $65^{\circ}C$, whereas the effective range of the dry heat treatment (DHT) was $60-65^{\circ}C$. Tolerance of mung bean seedlot, as estimated by hypocotyl elongation and root growth, was lower for HWT than for DHT. Germination and growth of sprouts were excellent over the range of $55-65^{\circ}C\;at\;5^{\circ}C$ intervals, except for HWT at $65^{\circ}C$ for 5 min. At this marginal condition, heat damage appeared so that approximately 2% of seeds failed to sprout to normal germling and retarded sprouts were less than 5% with coarse wrinkled hypocotyls. These results suggested that DHT would be more feasible to disinfect mung bean seedlots for commercial sprout production. Heat treatment at above ranges was highly effective in eliminating the epiphytic bacterial strains associated with marketed sprout rot samples. HWT of seedlot at 55 and $60^{\circ}C$ for 5 min resulted in successful control of mung bean sprout rot incidence with marketable sprout quality. DHT at 60 and $65^{\circ}C$ for 30 min also gave good results through the small-scale sprouting system. Therefore, we optimized DHT scheme at 60 and $65^{\circ}C$ for 30 min, considering the practical value of seedlot disinfection with high precision and accuracy. This was further proved to be a feasible and reliable method against anthracnose incidence and those bacterial strains associated with marketed sprout rot samples as well, through factory scale mung bean sprout production system.

Keywords

References

  1. Basim, E., Basim, H. and Ozcan, M. 2006. Antibacterial activities of turkish pollen and propolis extracts against plant bacterial pathogens. J. Food Eng. 77:992-996 https://doi.org/10.1016/j.jfoodeng.2005.08.027
  2. Biggs, A R. 1999. Effects of calcium salts on apple bitter rot caused by two Colletotrichum spp. Plant Dis. 83:1001-1005 https://doi.org/10.1094/PDIS.1999.83.11.1001
  3. Choi, Y M., Noh, D.O., Cho, S. Y, Suh, H. J., Kim, K. M. and Kim, J. M. 2006. Antioxidant and antimicrobial activities of propolis from several regions of Korea. LWT-Food Sci. Technol. 39:756-761 https://doi.org/10.1016/j.lwt.2005.05.015
  4. Conway, W. S., Leverentz, B., Saftner, R. A, Janisiewicz, W. J., Sarns, C. E. and Leblanc, E. 2000. Survival and growth of Listeria monocytogenes on fresh-cut apple slices and its interaction with Glomerella cingulata and Penicillium expansum. Plant Dis. 84:177-181 https://doi.org/10.1094/PDIS.2000.84.2.177
  5. Fallik, E. 2004. Prestorage hot water treatments. Postharvest Biol. Technol. 32: 125-134 https://doi.org/10.1016/j.postharvbio.2003.10.005
  6. Fett, W. F. and Cook, P. H. 2003. Native biofilms on mung bean sprouts. Can. J. Microbiol. 49:45-50 https://doi.org/10.1139/w03-002
  7. Han, K. S. and Lee, D. H. 1995. Identification and etiological characteristics of anthracnose fungi isolated from soybean, small red bean and green bean. Korean J. Plant Pathol. 11:30-38. (in Korean with English abstract)
  8. Kang, J. H., Ryu, Y S., Yoon, S. Y, Jean, S. H. and Cho, S. H. 2004a. Effect of aeration period and temperature after imbibition on growth of mung bean sprouts. Korean J. Crop Sci. 49:472-476. (in Korean)
  9. Kang, J. H., Ryu, Y S., Yoon, S. Y, Jeon, S. H. and Jeon, B. S. 2004b. Growth of mung bean sprouts and commodity temperature as affected by water supplying methods. Korean J. Crop Sci. 49:487-490. (in Korean)
  10. Kang, J. H., Ryu, Y. S., Yoon, S. Y, Jeon, S. H. and Kim, H. K. 2004c. Effect of benzyladenopurine soaking period on growth of mung bean sprouts. Korean J. Crop Sci. 49:477-481. (in Korean)
  11. Kang, J. H., Ryu, Y S., Yoon, S. Y, Jeon, S. H. and Kim, S. R. 2004d. Effect of benzyladenopurine concertration in soaking solution on growth of mung bean sprouts. Korean J. Crop Sci. 49:482-486. (in Korean)
  12. Kim, D. K., Lee, S. C, Kang, J. H. and Kim, H. K. 2003. Colletotrichum disease of mugbean sprout by Colletotrichum acutatum. Plant Pathol. J. 19:203-204 https://doi.org/10.5423/PPJ.2003.19.4.203
  13. Lee, H. B., Kwon, O., Kim, H., Kim, M. and Kim, C. J. 2003. Bioactivities of Korean Ginkgo (Ginkgo bilobo L.) extract and its potential as a natural pesticide. Res. Plant Dis. 9:99-103. (in Korean) https://doi.org/10.5423/RPD.2003.9.2.099
  14. Park, E. H. and Choi, Y. S. 1995. Selection of useful chemicals reducing soybean sprout rot. Korean J. Crop Sci. 40:487-493. (in Korean)
  15. Park, J. C., Song, W. Y and Kim, H. M. 1997a. Occurrence of bacterial soft sot of soybean sprout caused by Erwinia Carotovora subsp. carotovora. Korean J. Plant Pathol. 13:13-17. (in Korean)
  16. Park, W. M., Pyun, C. W. and Kim, J. H. 1997b. Bacterial rot of soybean sprout caused by saprophytic Pseudomonas putida biovar. A and control by acidity of water Korean J. Plant Pathol. 13:304-310. (in Korean)
  17. Weiss, A. and Hammes W. P. 2003. Thermal seed treatment to improve the food safety status of sprouts. J. Appl. Bot. 77: 152155
  18. Weiss, A. and Hammes, W. P. 2005. Efficacy of heat treatment in the reduction of salmonellae and Escherichia coli O157:H- on alfalfa, mung bean and radish seeds used for sprout production. Eur. Food Res. Technol. 221:187-191 https://doi.org/10.1007/s00217-004-1125-9

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