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Evaluation of DNA Extraction Methods from Low Copy Number (LCN) DNA Samples for Forensic DNA Typing

  • Eom, Yong-Bin
    • Biomedical Science Letters
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    • v.15 no.3
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    • pp.229-232
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    • 2009
  • DNA isolation for PCR-based short tandem repeat (STR) analysis is essential to recover high yields of amplifiable DNA from low copy number (LCN) DNA samples. There are different methods developed for DNA extraction from the small bloodstain and gloves, commonly found at crime scenes. In order to obtain STR profiles from LCN DNA samples, DNA extraction protocols, namely the automated $iPrep^{TM}$ $ChargeSwitch^{(R)}$ method, the automated $QIAcube^{TM}$ method, the automated $Maxwell^{(R)}$ 16 DNA $IQ^{TM}$ Resin method, and the manual $QIAamp^{(R)}$ DNA Micro Kit method, were evaluated. Extracted DNA was quantified by the $Quantifiler^{TM}$ Human DNA Quantification Kit and DNA profiled by $AmpFISTR^{(R)}$ $Identifiler^{(R)}$ Kit. Results were compared based on the amount of DNA obtained and the completeness of the STR profiles produced. The automated $iPrep^{TM}$ $ChargeSwitch^{(R)}$ and $QIAcube^{TM}$ methoas produced reproducible DNA of sufficient quantity and quality trom the dried blood spot. This two automated methods showed a quantity and quality comparable to those of the forensic manual standard protocols normally used in our laboratory. In our hands, the automated DNA extraction method is another obvious choice when the forensic case sample available is bloodstain. The findings of this study indicate that the manual simple modified $QIAamp^{(R)}$ DNA Micro Kit method is best method to recover high yields of amplifiable DNA from the numerous potential sources of LCN DNA samples.

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A Study on the Relationship between R&D Culture and Job Involvement in Information Technology Industries (정보기술산업의 R&D 문화와 직무몰입에 관한 연구)

  • Lee, Sun-Kyu;Lee, Ung-Hee;Jeon, Ki-Sang;Han, Soo-Deok
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.29 no.3
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    • pp.103-110
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    • 2006
  • This paper aims to examine the main factors of the organizational climate and analyze their effects on job involvement. To accomplish these purposes, the nam factors of the organizational climate such as diversity, autonomy, redundancy, connectivity and flexibility were found from the previous studies. The questionaries of 350 were distributed to the employees working at the Gumi Industrial Complex. The questionaries of 298 were obtained and 267 were analyzed through frequencies, correlation and multiple regression. The results of this study are as followings; First, we conducted exploratory factor analysis for five constructs(diversity, autonomy, redundancy, connectivity and flexibility) using oblique rotation method that did not assume independence among the factors and main factors of knowledge management were grouped together by five factors. Second, it is shown that four main factors of organizational climate have a positive influence on the job involvement(p<0.05). This study, however, have limitations of number of sample and survey area. The future study should consider these limitations in improving model applicability in practice.

AUTOSAR Starter Kit for AUTOSAR Software Design (AUTOSAR 소프트웨어 설계를 위한 실습 환경)

  • Lee, Seonghun;Kim, Youngjae;Kum, Daehyun;Jin, Sungho
    • IEMEK Journal of Embedded Systems and Applications
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    • v.9 no.2
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    • pp.87-99
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    • 2014
  • An AUTomotive Open System ARchitecture (AUTOSAR) is a de-facto standardized software platform, which developed for an automotive Electronic Control Unit (ECU) in global automotive industry. AUTOSAR improves the reusability and the scalability, thus the software development can be easier, faster and more reliable. However, it requires a lot of time and efforts to develop an AUTOSAR software due to the difficulties of understanding of massive AUTOSAR documentations and complicated usage of AUTOSAR design tools. AUTOSAR training is offered by AUTOSAR design tool venders but it is limited to introduction of their simplified concept and usages based on PC. Therefore the training is not enough for industrial developers or graduate students. In this paper we present an AUTOSAR starter kit which allows industrial engineers and graduate students to practice the detailed process of AUTOSAR software development easily and more conveniently. The kit is composed of a practical environment similar to actual automotive system and a textbook that explains how to design AUTOSAR software. And we demonstrated the validity of our methodology based on a case study.

A Project-Based Embedded Software Design Course (실습에 기반한 임베디드 소프트웨어 설계 교육)

  • Moon, Jung-Ho;Park, Lae-Jeong
    • Journal of the Korean Institute of Intelligent Systems
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    • v.21 no.5
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    • pp.581-587
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    • 2011
  • This paper presents a senior-level embedded software design course using a customized training kit. Embedded software design courses commonly entail a lot of practice hours and a semester-long project and thus requires a hardware platform on which the embedded software runs. A training kit has been designed such that both hardware system and operating system are not too complicated or heavy for undergraduate students to fully understand and to develop embedded software on their own. The course using the customized training kit gives the students hands-on experience of embedded software design and programming ranging from device drivers to user interface, thereby enabling them to have in-depth understanding of embedded software and to improve their programming skills more easily and faster than when using commercial training kits.

Influence of Partial Discharge Properties due to Void in Cable Joint Parts (케이블 접속재 부분방전 특성에 미치는 보이드의 영향)

  • 신종열;홍진웅
    • Journal of the Korean Society of Safety
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    • v.18 no.3
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    • pp.69-74
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    • 2003
  • To investigate the partial discharge and electric field distribution in cable joint parts, we measured the partial discharge and electric field in specimen. The specimens which cross-linked polyethylene(XLPE) and ethylene propylene diene ethylene(EPDM) are used to insulating material for underground cable md cable jointing parts. The polymers are used to insulating material in switchgear which is a kind of transformer equipment and in ultra-high voltage cable. Its using is increasing gradually, the electrical insulation properties are not only excellent but also mechanical property is excellent. And because it is possible to be made void of several type in insulator while it is produced, which the electrical field distribution is changed by void, it has a critical influence to insulator performance. The underground cable is connecting by the jointing material, insulating breakdown and the electric ageing which are caused by several mixing impurity and the damage of cable insulator layer, which reduced the life of cable while intermediate joint kit is connected. Therefore, the computer simulation is used to estimating insulator performance, XLPE is used to the insulating material of ultra-high voltage cable and EPDM is used to insulator layer in joint material kit, and which are produced as specimen. And it is analyzed the electric field concentrating distribution and partial discharge by modeling of computer simulation in void and cable joint kit.

Investigation of IZO/Al multilayer anode grown on PEN substrate by a twin target sputtering system for flexible top emitting organic light emitting diodes (TTS를 이용하여 PEN 기판 상에 성막한 플렉시블 전면 발광 OLED용 IZO/Al multilayer 애노드의 특성)

  • Oh, Jin-Young;Moon, Jong-Min;Jeong, Jin-A;Kim, Han-Ki
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2007.11a
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    • pp.444-445
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    • 2007
  • IZO/Al multilayer anode films for flexible top emitting organic light emitting diodes (TOLEDs) were grown on PEN (polyethylen-enaphthelate) substrate using twin target sputter (TTS) system. To investigate electrical and optical properties of IZO/Al multilayer films, 4-point probe method and UV/Vis spectrometer were used, respectively. From a IZO/Al multilayer films with 100nm-thick Al, sheet resistance of $1.4{\Omega}/{\square}$ and reflectance of above 62% at a range of 500~550nm wavelength could be obtained, In addition, structural and surface properties of IZO/Al multilayer films were analyzed by XRD (X-ray diffraction) and FESEM (field emission scanning electron microscopy) and AES (auger electron spectroscope), respectively. Moreover, flexibility of IZO/Al multilayer anode films were examined by bending test method.

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Rapid thermal annealing effect of IZO transparent conducting oxide films grown by a box cathode sputtering (박스캐소드 스퍼터로 성장시킨 IZO 투명 전도막의 급속 열처리 효과)

  • Bae, Jung-Hyeok;Moon, Jong-Min;Jeong, Soon-Wook;Kim, Han-Ki;Yi, Min-Soo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.06a
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    • pp.473-474
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    • 2006
  • We report on the rapid thermal annealing effect on the electrical, optical, and structural properties of IZO transparent conducting oxide films grown by box cathode sputtering (BCS). To investigate structural properties of rapid thermal annealed IZO films in $N_2$ atmosphere as a function of annealing temperature, syncrotron x-ray scattering experiment was carried out. It was shown that the amorphous structure of the IZO films was maintained until $400^{\circ}C$ because ZnO and $In_2O_3$ are immiscible and must undergo phase separation to allow crystallization. In addition, the IZO films grown at different Ar/$O_2$ ratio of 30/1.5 and 30/0 showed different preferred (222) and (440) orientation, respectively, with increase of rapid thermal annealing temperature. The electrical properties of the OLED with rapid thermal annealed IZO anode was degraded as rapid thermal annealing temperature of IZO increased. This indicates the amorphous IZO anode is more beneficial to make high-quality OLEDs.

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Characteristics of amorphous IZO anode based flexible organic light emitting diodes (비정질 IZO 애노드 박막을 이용한 플렉서블 유기발광소자 특성)

  • Moon, Jong-Min;Bae, Jung-Hyeok;Jeong, Soon-Wook;Kim, Han-Ki;Kang, Jae-Wook
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.06a
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    • pp.491-492
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    • 2006
  • We report on the fabrication of organic-based flexible display using an amorphous IZO anode grown at room temperature. The IZO anode films were grown by a conventional DC reactive sputtering on polycarbonate (PC) substrate at room temperature using a synthesized IZO target in a Ar/$O_2$ ambient. X-ray diffraction examination results show that the IZO anode film grown at room temperature is complete amorphous structure due to low substrate temperature. It is shown that the $Ir(ppy)_3$ doped flexible organic light emitting diode (OLED) fabricated on the IZO anode exhibit comparable current-voltage-luminance characteristics to OLED fabricated on conventional ITO/glass substrate. These findings indicate that the IZO anode film grown on PC substrate is a promising anode materials for the fabrication of organic based flexible displays.

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Thin Film Passivation of Organic Light Emitting Diodes by Catalyzer Enhanced Chemical Vapor Deposition (CECVD) (촉매반응 화학기상증착법을 이용한 유기발광소자의 박막 봉지)

  • Kim, Han-Ki;Moon, J.M.;Bae, J.H.;Jeong, S.W.;Kim, M.S.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.06a
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    • pp.71-72
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    • 2006
  • We report on plasma damage free chemical vapor deposition technique for the thin film passivation of organic light emitting diodes (OLEDs), organic thin film transistor (OTFT) and flexible displays using catalyzer enhanced chemical vapor deposition (CECVD). Specially designed CECVD system has a ladder-shaped tungsten catalyzer and movable electrostatic chuck for low temperature deposition process. The top emitting OLED with thin film $SiN_x$ passivation layer shows electrical and optical characteristics comparable to those of the OLED with glass encapsulation. This indicates that the CECVD technique is a promising candidate to grow high-quality thin film passivation layer on OLED, OTFT, and flexible displays.

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Torsional stress prediction of turbine rotor train using stress model (스트레스 모델을 이용한 터빈 축계의 비틀림 응력 예측)

  • Lee, Hyuk-Soon;Yoo, Seong-Yeon
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2013.10a
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    • pp.862-867
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    • 2013
  • Torsional interaction between electrical network phenomena and turbine-generator shaft cause torsional stress on turbine-generator shaft and torsional fatigue fracture on vulnerable component, but the prediction of the torsional stress is difficult because the torsional stress is occurred instantly and randomly. Therefore continuous monitoring of the torsional stress on turbine-generator shaft is necessary to predict the torsional fatigue, but installing the sensors on the surface of the shaft directly to monitor the stress is impossible practically. In this study torsional vibration was measured using magnetic sensor at a point of turbine-generator rotor kit, the torsional stress of whole train of rotor kit was calculated using rotor kit's stress model and the calculated results were verified in comparison with the measured results using strain gauge at several point of turbine-generator rotor kit. It is expected that these experiment results will be used effectively to calculate the torsional stress of whole train of turbine-generator rotor in power plants.

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