• Title/Summary/Keyword: KGD1

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Effect of KGD1 Deletion on Cell Wall Biogenesis in Saccharomyces cerevisiae (Saccharomyces cerevisiae의 KGD1 유전자 결손이 세포벽 생합성에 미치는 영향)

  • Kim, Sung-Woo;Ahn, Ki-Woong;Park, Yun-Hee;Park, Hee-Moon
    • The Korean Journal of Mycology
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    • v.38 no.1
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    • pp.29-33
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    • 2010
  • KGD1 gene was cloned by functional complementation of defects in $\beta$-1,3-glucan synthase activity of the previously isolated Saccharomyces cerevisiae mutant LP0353, which displays a number of cell wall defects at restrictive temperature. We performed the gene disruption experiment to characterize the function of KGD1 gene, which encodes $\beta$-ketoglutarate dehydrogenase, in cell wall biosynthesis. The disruption of KGD1 showed the decreased growth rate, the increase of chitin synthases activity, alterations in cell wall composition, and increase of susceptibility to cell wall inhibitors such as Calcofluor white and Nikkomycin Z. These results suggested that KGD1 might be involved in cell wall biogenesis, especially the biosynthesis of $\beta$-1,6-glucan and chitin in S. cerevisaie.

Integrated GPS Network Adjustment for Determining KGD2002 Coordinate Sets (통합 GPS망조정에 의한 삼각점의 세계측지계 성과결정 연구)

  • Lee, Young-Jin;Jung, Kwang-Ho
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.31 no.1D
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    • pp.167-173
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    • 2011
  • This paper analyzes the procedure of calculating how the results of national control points (triangulation points), based on Bessel datum, have been transformed into those of KGD2002, based on the world geodetic system. GPS and EDM data observed from 1974 to 2008 were used for this purpose. A large-scale integrated GPS network was constructed to estimate the results of KGD2002 and new national control points about 12,000 were decided through multiple stages of data processing. The accuracy of these results is ${\pm}0.015m$ (95%) in the horizontal direction and ${\pm}0.030m$ (95%) in the vertical direction. The adjusted results verified by the construction of an integrated GPS/EDM network were compared with the results of KGD2002. In conclusion, the bulletin results are thought to be appropriate because the coordinate differences (RMSE) are ${\pm}0.0025m$ and ${\pm}0.008m$ in horizontal and vertical directions respectively.

Realization of New Korean Horizontal Geodetic Datum: GPS Observation and Network Adjustment

  • Lee, Young-Jin;Lee, Hung-Kyu;Jung, Gwang-Ho
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • v.1
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    • pp.529-534
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    • 2006
  • New geocentric geodetic datum has recently been realized in Korea, Korean Geodetic Datum 2002- KGD2002, to overcome problems due to the existing Tokyo datum, which had been used in this country since early 20th century. This transition will support modern surveying techniques, such as Global Navigation Satellite Systems (GNSS) and ensures that spatial data is compatible with other international systems. For this realization, very long baseline interferometry (VLBI) observations were initially carried out in 1995 to determine the coordinates of the origin of KGD2002 based on the International Terrestrial Reference Frame (ITRF). Continuous GPS observations were collected from 14 reference stations across Korea to compute the coordinates of 1st order horizontal geodetic control points. During the campaign, GPS observations were also collected at about 9,000 existing geodetic control points. In 2006, network adjustment with all data obtained using GPS and EDM since 1975 has been performed under the condition of fixing the coordinates of GPS continuous observation stations to compute coordinate measurements of the 2nd and 3rd geodetic control points. This paper describes the GPS campaigns which have been undertaken since 1996 and details of the network adjustment schemes. This is followed

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High Speep/High-Precision Chip Joining Using Self-Assembly Technology for Three-Dimensional Integrated Circuits (삼차원적층형 집적회로 구현을 위한 자기조직화정합기술을 이용한 고속.고정밀 접합기술)

  • Lee, Kang-Wook
    • Journal of Welding and Joining
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    • v.29 no.3
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    • pp.19-26
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    • 2011
  • 본 논문에서는 액체의 표면장력을 이용하여 복수의 KGD 들을 웨이퍼 상태에서 일괄접합함으로써, 높은 수율의 삼차원적층칩을 빠른 생산성으로 제작할 수 있는, 고속 고정밀 접합기술인 자기조직화정합 (Selfassembly) 기술에 대해 소개를 하였다. 본 연구실에서 개발한 self-assembly 기술을 적용하여 5mm 각(角) 크기의 칩 500개를 1초 이내에 평균 $0.5{\mu}m$ 정도의 높은 정밀도로 8인치 웨이퍼상에 일괄접합시키는데 성공하였다. Self-assembly 기술에 의한 삼차원 칩 적층방식은, 기존의 pick-and-place 적층방식에서 높은 정밀도의 접합특성을 확보하는데 필요한 공정시간을 혁신적으로 단축하는 것이 가능하고, 웨이퍼 레벨에서 복수의 KGD 들을 일괄접합하는 것이 가능하므로, 향후 TSV 기술의 양산화를 실현하는데 적합한 고속 고정밀 접합 기술로서 기대가 크다. 현재 본 연구실에서는 두께가 $50{\mu}m$ 이하의 얇은 LSI 칩 및 메탈범프가 형성된 LSI 칩 등을 이용하여, self-assembly 기술에 의한 삼차원 적층형 집적회로 구현을 위한 접합기술을 개발 중에 있다.

GPS Network Adjustment for Determining KGD2002 Coordinates of the $2^{nd}$ Order Geodetic Control Points (GPS망조정에 의한 2등측지기준점의 세계측지계 성과산정)

  • Lee, Young-Jin;Lee, Hyung-Kyu;Jeong, Gwang-Ho;Lee, Jun-Hyuk
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.25 no.5
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    • pp.451-463
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    • 2007
  • This paper describes issues of GPS network adjustment to determine coordinate sets of the $2^{nd}$ order national geodetic control points based on the Korean Geodetic Datum (KGD2002) which has been newly adopted in 2003, After outlining theoretical background of the GPS network processing, the adjustment procedure applied for this project is detailed. Throughout performing a series of minimally constrained adjustments, some outliers have been removed and magnitude of absolute and relative error for a stochastic modeling has been determined as 4mm+0.4ppm and 8mm+0.8ppm in the horizontal and vertical component, respectively. The over constrained adjustment by fixing the $1^{st}$ order control points was performed to derive final solution, indicating that the accuracy of the estimated coordinates was 2cm and 4cm in the horizontal and vertical component.

The 3rd order GPS Network Adjustment to Determine KGD2002 Coordinate Sets (GPS망조정에 의한 세계측지계의 3등기준점 성과산정)

  • Lee, Young-Jin;Lee, Hung-Kyu;Jeong, Kwang-Ho;Song, Jun-Ho
    • Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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    • 2007.04a
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    • pp.81-84
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    • 2007
  • This paper describes general procedure and results of the GPS 3rd odor network adjustment which has been carried out for determining coordinates sets with respect to new Korean Geodetic Datum, so-call Korean Geodetic Datum 2002 (KGD 2002). The adjustment begins with minimally constrained adjustments with respect to each of the 69 campaign networks. This was followed by constructing and adjusting sixteen block network. After detecting and removing outliers in the observation file, an attempt was made by applying the empirical stochastic modeling techniques used in the 2nd order network adjustment, so as to determine the magnitude of absolute and relative error for the estimated baseline vector from the GPS data processing. The over constrained adjustment were, in sequence, performed against each of the block network. In this adjustment, both of the 2nd order control points in the block network and the 3rd order control points overlapped with adjacent network whose coordinates were already determined from a preceding adjustment. The final adjustment results have shown that the accuracy of the 3rd order network adjustment was better than 1cm and 2cm in horizontal and vertical component, respectively.

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OK-KGD:Open-domain Korean Knowledge Grounded Dialogue Dataset (OK-KGD:오픈 도메인 한국어 지식 기반 대화 데이터셋 구축)

  • Seona Moon;San Kim;Jinyea Jang;Minyoung Jeung;Saim Shin
    • Annual Conference on Human and Language Technology
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    • 2023.10a
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    • pp.342-345
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    • 2023
  • 최근 자연어처리 연구 중 오픈 도메인 지식 기반 대화는 많은 관심을 받고 있다. 연구를 위해서는 오픈 도메인 환경을 갖추고 적절한 지식을 사용한 대화 데이터셋이 필요하다. 지금까지 오픈 도메인 환경을 갖춘 한국어 지식 기반 대화 데이터셋은 존재하지 않아 한국어가 아닌 데이터셋을 한국어로 기계번역하여 연구에 사용하였다. 이를 사용할 경우 두 가지 단점이 존재한다. 먼저 사용된 지식이 한국 문화에 익숙하지 않아 한국인이 쉽게 알 수 없는 대화 내용이 담겨있다. 그리고 번역체가 남아있어 대화가 자연스럽지 않다. 그래서 본 논문에서는 자연스러운 대화체와 대화 내용을 담기 위해 새로운 오픈 도메인 한국어 지식 기반 대화 데이터셋을 구축하였다. 오픈 도메인 환경 구축을 위해 위키백과와 나무위키의 지식을 사용하였고 사용자와 시스템의 발화로 이루어진 1,773개의 대화 세트를 구축하였다. 시스템 발화는 크게 지식을 사용한 발화, 사용자 질문에 대한 답을 주지 못한 발화, 그리고 지식이 포함되지 않은 발화 3가지로 구성된다. 이렇게 구축한 데이터셋을 통해 KE-T5와 Long-KE-T5를 사용하여 간단한 실험을 진행하였다.

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Molecular Identification of Korean Mountain Ginseng Using an Amplification Refractory Mutation System (ARMS)

  • In, Jun-Gyo;Kim, Min-Kyeoung;Lee, Ok-Ran;Kim, Yu-Jin;Lee, Beom-Soo;Kim, Se-Young;Kwon, Woo-Seang;Yang, Deok-Chun
    • Journal of Ginseng Research
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    • v.34 no.1
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    • pp.41-46
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    • 2010
  • Expensive herbs such as ginseng are always a possible target for fraudulent labeling. New mountain ginseng strains have occasionally been found deep within mountain areas and commercially traded at exorbitant prices. However, until now, no scientific basis has existed to distinguish such ginseng from commonly cultivated ginseng species other than by virtue of being found within deep mountain areas. Polymerase chain reaction (PCR) analysis of the internal transcribed spacer has been shown to be an appropriate method for the identification of the most popular species (Panax ginseng) in the Panax ginseng genus. A single nucleotide polymorphism (SNP) has been identified between three newly found mountain ginseng (KGD4, KGD5, and KW1) and already established Panax species. Specific PCR primers were designed from this SNP site within the sequence data and used to detect the mountain ginseng strains via multiplex PCR. The established multiplex-PCR method for the simultaneous detection of newly found mountain ginseng strains, Korean ginseng, and foreign ginseng in a single reaction was determined to be effective. This study is the first report of scientific discrimination of "mountain ginsengs" and describes an effective method of identification for fraud prevention and for uncovering the possible presence of other, cheaper ginseng species on the market.

The $3^{rd}$ Order GPS Network Adjustment for Determining of KGD2002 Coordinate Sets (GPS망조정에 의한 3등측지기준점의 세계측지계 성과산정)

  • Lee, Young-Jin;Jeong, Kwang-Ho;Lee, Hung-Kyu;Kwon, Chan-Oh;Song, Jun-Ho;Cho, Jun-Rae;Nam, Gi-Beom;Cha, Sang-Heon
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.25 no.5
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    • pp.437-449
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    • 2007
  • This paper deals with issues of GPS network adjustment for the purpose of determining the $3^{rd}$ order national geodetic control points based on the Korean Geodetic Datum which has newly adopted in the country since early 2003. After examining and arranging all the observations, GPS baseline analyses were performed to estimate baseline vectors between two control points. All the observations were divided into 17 block networks in order to accelerate the adjustment efficiency. After applying a minimally constrained adjustment technique to each of the block networks for the sake of detecting outliers and examining network precision, over constrained adjustments by fixing all of the $2^{nd}$ order control points within the block network were carried out to derive final coordinate sets. The final solutions indicated that the accuracy of the adjusted coordinates was better than 1cm and 2cm in the horizontal and vertical component, respectively.

Ultimate Heterogeneous Integration Technology for Super-Chip (슈퍼 칩 구현을 위한 헤테로집적화 기술)

  • Lee, Kang-Wook
    • Journal of the Microelectronics and Packaging Society
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    • v.17 no.4
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    • pp.1-9
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    • 2010
  • Three-dimensional (3-D) integration is an emerging technology, which vertically stacks and interconnects multiple materials, technologies, and functional components such as processor, memory, sensors, logic, analog, and power ICs into one stacked chip to form highly integrated micro-nano systems. Since CMOS device scaling has stalled, 3D integration technology allows extending Moore's law to ever high density, higher functionality, higher performance, and more diversed materials and devices to be integrated with lower cost. The potential benefits of 3D integration can vary depending on approach; increased multifunctionality, increased performance, increased data bandwidth, reduced power, small form factor, reduced packaging volume, increased yield and reliability, flexible heterogeneous integration, and reduced overall costs. It is expected that the semiconductor industry's paradiam will be shift to a new industry-fusing technology era that will offer tremendous global opportunities for expanded use of 3D based technologies in highly integrated systems. Anticipated applications start with memory, handheld devices, and high-performance computers and extend to high-density multifunctional heterogeneous integration of IT-NT-BT systems. This paper attempts to introduce new 3D integration technologies of the chip self-assembling stacking and 3D heterogeneous opto-electronics integration for realizng the super-chip.