• Title/Summary/Keyword: KD

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Action of Protein Kinase A and C Activators on Germinal Vesicle Breakdown and One-Cell Embryos in the Mouse (생쥐 GV난자와 1-세포기 배아의 핵막붕괴에 미치는 Protein Kinase A와 C의 작용)

  • 이대기;김경진;조완규
    • The Korean Journal of Zoology
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    • v.32 no.2
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    • pp.153-162
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    • 1989
  • Expedments were perfonned to examine the role of cAMP-dependent protein kinase (PK-A) and diacylglycerol-dependent protein kinase (PK-C) during the meiodc resumption and the first mitotic cell cycle of mouse embryogenesis. Mejoric GV oocytes and one-cell embryos derived from in vitro fertilization were cultured in vitro, and morphological changes in response to activators of PK-A and PK-C were examined. Treatments with a membrane-permeable cAMP analog, dbcAMP (0.1 mg/mi), phosphodiesterase inhibitor, IBMX (0.1 mM), biologically active phorbol ester, WA (10 nglmi), or a synthetic diacylglycerol, sn-diC8 inhibited resumption of melosis. Combination of PK-A and PK-C activator brought about furiher inhibition. On the contrary, dbcAMP (0.1 mg/mi), IBMX (0.2 mM), WA (10 nglml), and sn-diC8 (0.5 mM) did not inhibit pronucleus membrane breakdown (PNBD) when added S or G2 phase of cell cycle. However, activators of PK-C inhibited cleavage of one-cefl embryos. This result indicates that the action mechanism of PK-A and PK-C on dissolution of nuclear membrane in primary meiotic arrest oocytes may be different from that of mitotic one-cell embryos.

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Wheel Load Distribution of Continous Reinforced Concrete Slab Bridge (연속 철근콘크리트 슬래브 교량의 윤하중 분포폭에 관한 연구)

  • 신호상;오병환
    • Magazine of the Korea Concrete Institute
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    • v.10 no.4
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    • pp.135-143
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    • 1998
  • The wheel load distribution width for lane load is not specified in current Korea bridge design code(KD code), not like in current AASHTO and AASHTO LRFD specifications which specity it as twice of wheel load distribution width for wheel load. In this study, the wheel load distribution width in continuous reinforced concrete slab bridge is investigated. The major variables affecting the wheel load distribution of a reinforced concrete continuous slab bridge are the span length, bridge width, existence edge beam and boundary condition. From a series of comprehensive parametric study on each variable, the formula for wheel load distribution in continuous reinforced concrete slab bridge is proposed from the nonlinear regression analysis of finite element analysis results. The proposed formulas can be used efficiently in the accurate design of continuous reinforced concrete slab bridges.

Home Range Analysis of Great Tit (Parus major) before and after Fledging in an Urban Park (도시공원에 번식하는 박새의 이소 전후 어미 행동권 분석)

  • Song, Won-Kyong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.23 no.1
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    • pp.97-106
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    • 2020
  • Urban parks provide a variety of ecosystem services and are an important means of providing positive functions to urban ecosystems. Recently, various studies on wildlifes in urban parks have been conducted. However, there is a lack of research on habitat use in urban parks at important times such as before and after fledging in bird ecology. This study analyzed habitat use and home-range before and after fledging on Cheongsa park, a neighborhood park located in Cheonan city. An artificial nest was set up to check and capture great tit in fledging time. One female was captured and attached to the NTQB-2 (0.4g) radio transmitter, the location was tracked using SIKA Radio Tracking Receiver, hand-held three element Yagi antenna and GPS. Location information was recorded for 10 minutes for 3 hours each morning and afternoon for 12 days from May 17 to May 31, 2019. As a result, the home-range of the target species was 1.776 ha (MCP) and the core area was 499 ㎡ (KD 50%). The average daily home-range was 0.513 ha for the entire period, 0.688 ha before fledging, 0.339 ha after fledging based on MCP. The bird moved about 29.9 m on average and moved up to 131.7 m. For the most of the time, the great tit stayed inside the park, but the bird also used small green spaces such as street trees, tree flower beds, and green areas of unused lands. The results of this study could be applied to the study of habitat use and the greenery management policy of the urban park considering wild birds.

Biosphere Modeling for Dose Assessment of HLW Repository: Development of ACBIO (고준위 방사성패기물 처분장 생태계 모델링을 위한 ACBIO개발)

  • Lee, Youn-Myoung;Hwang, Yong-Soo
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.6 no.2
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    • pp.73-100
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    • 2008
  • For the purpose of evaluating dose rate to individual due to long-term release of nuclides from the HLW repository, a biosphere assessment model and the implemented code, ACBIO, based on BIOMASS methodology have been developed by utilizing AMBER, a general compartment modeling tool. To show its practicability and usability as well as to see the sensitivity of compartment scheme or parametric variation to concentration and activity in compartments as well as annual flux between compartments at their peak values, some calculations are made and investigated: For each case when changing the structure of compartments and GBIs as well as varying selected input Kd values, all of which seem very important among others, dose rate per nuclide release rate is separately calculated and analyzed. From the maximum dose rates (Bq/y), flux-to-dose conversion factors (Sv/Bq) for each nuclide were derived, which are to be used for converting the nuclide release rate appearing from the geosphere through various GBIs to dose rate (Sv/y) for individual in critical group. It has been also observed that compartment scheme, identification of possible exposure group and GBIs could be all highly sensitive to the final consequences in biosphere modeling.

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Generation characteristics of transparent BIPV module according to temperature change (건물일체형 투명 모듈의 온도 변화에 따른 발전 특성)

  • Park, Kyung-Eun;Kang, Gi-Hwan;Kim, Hyun-Il;Yu, Gwon-Jong;Jang, Dae-Ho;Lee, Moon-Hee;Kim, Jun-Tae
    • Proceedings of the KIEE Conference
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    • 2007.07a
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    • pp.210-211
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    • 2007
  • Amid booming PV(photovoltaic) industry, BIPV(Building Integrated PV) is one of the best fascinating PV application technologies. To apply PV in building, variable factors should be reflected such as installation position, shading, temperature effect and so on. Especially a temperature should be considered, for it affects both electrical efficiency of PV module and heating and cooling load in building. Transparent PV modules were designed as finished material for spandrels are presented in this paper. The temperature variation of the modules with and without air gap and insulation were compared and analyzed. The results showed that the module with air gap and insulation has a much larger temperature variation than another transparent module. The temperature of the module reached by 55degree C under vertical irradiance of lower 500$W/m^2$. And the temperature difference between these modules was about 15degree C. To analyze the output performance of module according to temperature variation, separate module was manufactured and measured by sun-simulator. The results showed that 1 degree temperature rise reduced about 0.45% of output power.

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Study of the effects of injector cleaning on the exhaust gases in a common rail diesel engine (커먼레일 디젤엔진의 인젝터 클리닝이 배기가스에 미치는 영향에 관한 연구)

  • Cho, Hong-Hyun;Kim, Tae-Jung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.10
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    • pp.5980-5987
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    • 2014
  • As a response to exhaust gas regulations, the electronic control system was applied to the diesel engine. The injected fuel mass and injection timing are accurately controlled using it, and the fuel efficiency and the engine output are significantly increased. In addition, the noise and the vibration of vehicles are decreased. To maintain the optimal performance of an electronic control diesel engine, it is important to control the fuel injection pressure accurately using the fuel pressure regulator. When the fuel pressure regulator is not worked normally, the failure phenomena (starting failure, staring delay, accelerated failure, engine mismatch et al.) occurred because the fuel pressure is not stabilized and controlled accurately. In this study, the effects on a fuel pressure, return fuel mass flow, and engine rotating speed according to the control rate of fuel pressure regulator were investigated to analyze the performance variation under the failure conditions of a fuel pressure regulator. As a result, when the control rate of a fuel pressure regulator decreased by 4%~6% compared to that of the standard condition, the variation of engine rotating speed and return fuel flow were increased greatly, and the abnormal condition occurred. In addition, it is possible to diagnose the failure of a fuel pressure regulator by monitoring these conditions.

Difference of Gene Expression between Hypertrophic Scar Keratinocytes and Normal Keratinocytes (비후성 반흔 각질세포와 정상 각질세포의 유전자 비교분석)

  • Choi, Sung-Won;Chung, Ho-Yun;Lim, Young-Kook;Kim, Hoon-Nam;Oh, Ji-Won;Kim, Moon-Kyu;Jeon, Sae-Hwa;Hong, Yong-Taek
    • Archives of Plastic Surgery
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    • v.37 no.4
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    • pp.317-322
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    • 2010
  • Purpose: There is no clear evidence of the original cause of hypertrophic scar, and the effective method of treatment is not yet established. Recently the steps of searching in gene and molecular level are proceeding. we are trying to recognize the difference between keratinocytes of hypertrophic scar and normal skin. Then we do support the comprehension of the scar formation mechanism and scar management. Methods: Total RNAs were extracted from cultured keratinocytes from 4 hypertrophic scars and normal skins. The cDNA chips were prepared. A total of 3063 cDNAs from human cDNA library were arrayed. And the scanning data were analyzed. Results: On microarray, heat shock protein, pyruvate kinase, tumor rejection antigen were more than 2 fold intensity genes. Among them, heat shock 70 kd protein showed the strongest intensity difference. Conclusion: In this study, it can be concluded that heat shock proteins play an important role in the process of wound healing and scar formation. This study provides basic biologic information for scar research. The new way of the prevention and treatment of scar formation would be introduced with further investigations.

Estimation of SOA Weighting Factors for Radar Rainfall Adjustment and Evaluation of Hydrologic Accuracy (레이더강우 보정을 위한 SOA방법의 가중치 산정 및 수문학적 정확성 평가)

  • Noh, Hui-Seong;Kang, Na-Rae;Shin, Hyun-Seok;Kim, Byung-Sik;Kim, Hung-Soo
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.155-155
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    • 2012
  • 최근 집중호우에 따른 자연재해가 크게 증가하여 수문 및 기상분야에서 강우를 관측 및 예측하기 위한 레이더 활용성이 증대되고 있으며, 이에 따라 정부 각 관련부처에서는 레이더의 도입 및 확충을 위한 방안을 계획 제시하고 있다. 특히, 레이더강우 자료는 수문해석분야에서의 GIS 등 디지털정보를 이용하여 유역매개변수를 추정함에 따라 더 정확하고 상세한 수문자료 확보가 가능하게 되어, 격자기반의 분포형 강우-유출모형 등을 이용하여 수문해석을 하는데 있어 그 활용성이 크게 증가하고 있다. 그러나 레이더강우 자료의 정확성은 아직까지 만족할 만한 수준에 이르지 못하고 있기 때문에, 그 불확실성으로 인하여 레이더강우 자료의 활용 및 적용에 한계를 가지고 있는 실정이다. 본 연구에서는 지상강우를 이용한 레이더강우 보정을 위하여 SOA(Statistical Objective Analysis)보정방법을 이용하였으며, 기존 SOA방법에서의 거리에 따른 가중치($_{kd}$)와 함께 지형(고도)에 따른 가중치($W_{ike}$), 바람의 영향에 따른 가중치($W_{ikw}$)를 추가로 산정하여 적용하였으며, 이를 통하여 보정된 강우장을 생성하였다. SOA방법을 통해 생성된 강우장이 어느 정도의 강우정확도를 가지고 강우분포를 재현하는지 검증하기 위하여 2011년 7~9월의 수문학적 분석에 활용 가능한 강우사상과 낙동강유역을 대상으로, 분포형모형인 $Vflo^{TM}$ 모형을 이용하여 산정된 유출량과 관측 유출량과의 비교를 실시하였다. 또한, 유출량에 대한 오차 및 ME(Model Efficiency)를 통해 레이더강우 자료의 유출모형에서의 효율성을 검토하고자 하였다. 본 연구에서 수행된 보정 강우장에 따른 유출량 비교를 통해 레이더 강우의 정확도에 대한 정량적 평가 방법 도출이 가능할 것으로 판단된다.

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Study on infection of Theileria sergenti in neonatal calves (신생 송아지에 있어서 Theileria sergenti 의 감염에 관한 연구)

  • Baek, Byeong-kirl;Rim, Byung-moo;Lee, Woo-jong;Kim, Jin-ho;Kim, Byeong-su;Son, Dong-soo;Lee, Kwang-won
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.665-671
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    • 1993
  • The rate of 67 neonatal calves's infection of Theileria sergenti was investigated in random samples on the farms located in Kyeongki, Chonbuk and Jeju districts of Korea. The criteria used in verifying infection with T sergenti included the detection of parasites by giemsa's stain and acridine orange stain in the blood smear slides. Further evidence of current or previous exposure to T sergenti was based on the demonstration of T sergenti-specific antibody and antigen by the western immunoblot and the indirect immunofluorescence antibody test in the peripheral blood of the calves. The prevalence rates were 35%, 50% and 100% in Kyeongki, Chonbuk and Jeju provinces respectively and the overall prevalence in all the farms was 43.2% by means of acridine orange stain. The parasites that were observed in the peripheral blood of calves was shown surely by the western immunoblot to the characteristic 34KD antigen among the proteins of T sergenti (Korean Isolate). And the antibody of the neonatal calves reacted at the very highest titer(1 : >2,520). These data highlight the significance of T sergenti in the neonatal calf disease in Korea.

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Adsorption and Activity Changes of Trifluralin by Clay Minerals (점토광물(粘土鑛物)에 의한 Trifluralin의 흡착(吸着) 및 활성변화(活性変化))

  • Lee, Jyung Jae;Choi, Jyung
    • Current Research on Agriculture and Life Sciences
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    • v.2
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    • pp.63-67
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    • 1984
  • This study was carried out to know the Trifluralin adsorption experiment was determined with slurry method and the bioassay was conducted with barley seedlings. The adsorption of Trifluralin on clay minerals nearly was reached to eqilibrium concentration by shaking for 18 hours. The more the amount of clay minerals, the more Trifluralin was adsorbed by clay minerals. However the amount of Trifluralin adsorption per gram of clay minerals was reduced. The amount of Trifluralin adsorbed was higher on Montmorillonite than Zeolite and Kaolinite. And Kd value of Trifluralin was the greatest on the Montmorillonite.

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